2016 Vol. 32, No. 5

  Previous Issue | Next Issue 
Display Method:
Research Status and Development Review of Translational Medicine of Traditional Chinese Medicine
CHENGHai-bo, SHENWei-xing, WUMian-hua, SUNDong-dong
2016, 32(5): 401-404.
[Abstract](996) [PDF 1050KB](612)
Abstract:
Being a brand new medical theory and practice pattern， translational medicine represents the development tendency and inevitable requirement of modern medical science， which emphasizes the two-way rapid transformation between basic research and clinical application. Based on the relationship between traditional Chinese medicine and translational medicine， ideas and strategies for basic research of Chinese herbal compound are put forward by elaborating functions of Chinese herbal compound in translational medicine of traditional Chinese medicine and exploring its proper ways to serve clinical practice， which is not only beneficial to the innovation of traditional Chinese medicine theory and development of new clinical effective medicine， but also conductive to the discovery of new mechanisms and approaches for disease prevention and treatment as well as the illumination of principles and rules for herbal compatibility.
Research Status and Development Review of Translational Medicine of Traditional Chinese Medicine
CHENGHai-bo, SHENWei-xing, WUMian-hua, SUNDong-dong
2016, 32(5): 401-404.
[Abstract](1041) [PDF 390KB](1007)
Abstract:
Being a brand new medical theory and practice pattern， translational medicine represents the development tendency and inevitable requirement of modern medical science， which emphasizes the two-way rapid transformation between basic research and clinical application. Based on the relationship between traditional Chinese medicine and translational medicine， ideas and strategies for basic research of Chinese herbal compound are put forward by elaborating functions of Chinese herbal compound in translational medicine of traditional Chinese medicine and exploring its proper ways to serve clinical practice， which is not only beneficial to the innovation of traditional Chinese medicine theory and development of new clinical effective medicine， but also conductive to the discovery of new mechanisms and approaches for disease prevention and treatment as well as the illumination of principles and rules for herbal compatibility.
Relationship Between Visceral Differentiation and Syndrome Factor Differentiation
YANG Tao, XU Zheng, WU Cheng-yu
2016, 32(5): 405-408.
[Abstract](1100) [PDF 1102KB](767)
Abstract:
Relationship between visceral differentiation and syndrome factor differentiation was explored and contrasted from the following five perspectives: theory knowledge， principles of syndrome differentiation， division of disease location， thinking pattern， specifications of syndrome names. It is proved that there existed similarities between them in theory knowledge and principles of syndrome differentiation and the establishment of a new unified system for syndrome differentiation was put forward， with disease location and disease character being the core of syndrome differentiation. While the differences were detected between the two in the division of disease location， thinking pattern and specifications of syndrome names， for the disease location of visceral differentiation was divided based on the five zang-organs system and the differentiation was made in accordance with disease character， with both common basic syndrome and compound syndrome being summarized. Hence， the syndrome was expounded in a more logical and systematic way. While syndrome factor differentiation was made by following the pattern of “ syndrome-syndrome factors-syndrome names” and the disease location was divided by time-space， with weighted-sum threshold logic operation and syndrome differentiation scale being applied， reaching the results of quantizing information and providing stronger operability. Visceral differentiation is the further development and innovation of syndrome factor differentiation， with a more systemic division of disease location， more logical thinking pattern and more specifications of syndrome names. Hence， combining different syndrome differentiation methods together will promote the development of modern theoretical system of traditional Chinese medicine syndrome differentiation.
Relationship Between Visceral Differentiation and Syndrome Factor Differentiation
YANG Tao, XU Zheng, WU Cheng-yu
2016, 32(5): 405-408.
[Abstract](877) [PDF 422KB](1017)
Abstract:
Relationship between visceral differentiation and syndrome factor differentiation was explored and contrasted from the following five perspectives: theory knowledge， principles of syndrome differentiation， division of disease location， thinking pattern， specifications of syndrome names. It is proved that there existed similarities between them in theory knowledge and principles of syndrome differentiation and the establishment of a new unified system for syndrome differentiation was put forward， with disease location and disease character being the core of syndrome differentiation. While the differences were detected between the two in the division of disease location， thinking pattern and specifications of syndrome names， for the disease location of visceral differentiation was divided based on the five zang-organs system and the differentiation was made in accordance with disease character， with both common basic syndrome and compound syndrome being summarized. Hence， the syndrome was expounded in a more logical and systematic way. While syndrome factor differentiation was made by following the pattern of “ syndrome-syndrome factors-syndrome names” and the disease location was divided by time-space， with weighted-sum threshold logic operation and syndrome differentiation scale being applied， reaching the results of quantizing information and providing stronger operability. Visceral differentiation is the further development and innovation of syndrome factor differentiation， with a more systemic division of disease location， more logical thinking pattern and more specifications of syndrome names. Hence， combining different syndrome differentiation methods together will promote the development of modern theoretical system of traditional Chinese medicine syndrome differentiation.
Collateral Disease Theory of YE Tianshi and Its Application on Miscellaneous Diseases Due to Internal Injury
GE Hui-nan
2016, 32(5): 409-412.
[Abstract](969) [PDF 1039KB](984)
Abstract:
Since Ye Tianshi is a representative of Wumen Medical School， this paper systemically summarizes Dr. Ye’s thoughts on collateral disease theory and its application in the clinical practice by analyzing his master work Case Records as a Guide to Clinical Practice， where he puts forward the famous ideas of long illnesses entering into collaterals and long pains into collaterals， with etiology and pathogenesis of collateral diseases being expounded and treatment principles of dredging collaterals with herbs of spicy flavor， ants and insects or by removing blood stasis as well as prescriptions being advocated on the basis of experience from predecessors. Hence， a deep exploration of his collateral disease theory plays a significant role in improving the clinical curative effects on miscellaneous diseases.
Collateral Disease Theory of YE Tianshi and Its Application on Miscellaneous Diseases Due to Internal Injury
GE Hui-nan
2016, 32(5): 409-412.
[Abstract](964) [PDF 415KB](1254)
Abstract:
Since Ye Tianshi is a representative of Wumen Medical School， this paper systemically summarizes Dr. Ye’s thoughts on collateral disease theory and its application in the clinical practice by analyzing his master work Case Records as a Guide to Clinical Practice， where he puts forward the famous ideas of long illnesses entering into collaterals and long pains into collaterals， with etiology and pathogenesis of collateral diseases being expounded and treatment principles of dredging collaterals with herbs of spicy flavor， ants and insects or by removing blood stasis as well as prescriptions being advocated on the basis of experience from predecessors. Hence， a deep exploration of his collateral disease theory plays a significant role in improving the clinical curative effects on miscellaneous diseases.
Exploration of Zeqi Decoction in Treating Cough and Asthma due to Phlegm and Retained Fluid
LEIKun-peng, ZHUYong-zhong, WUJie
2016, 32(5): 413-415.
[Abstract](1116) [PDF 1026KB](972)
Abstract:
Zeqi Decoction is originated from Synopsis of Golden Chamber effective in curing cough and asthma due to phlegm and retained fluid， which has not received enough attention for a long time because of its simple description. Both action mechanism of this formula and its clinical practical value are revealed by analyzing the mechanism of cough and asthma due to phlegm and retained fluid as well as its clinical manifestation， herbal compatibility and Function characteristics. It is proved that Zeqi Decoction shares the similar function with Shizao Decoction， Kongxian Pill in draining water and removing phlegm. However， apart from mainly efficient in dispelling pathogens like activating yang， relieving retained fluid， lowering the adverse qi to stop cough， Zeqi Decoction is also able to support the healthy qi， making itself suitable to to treat cough and asthma due to phlegm-fluid lying latent in the lung or fluid retained in chest and hypochondrium.
Exploration of Zeqi Decoction in Treating Cough and Asthma due to Phlegm and Retained Fluid
LEIKun-peng, ZHUYong-zhong, WUJie
2016, 32(5): 413-415.
[Abstract](1041) [PDF 367KB](1240)
Abstract:
Zeqi Decoction is originated from Synopsis of Golden Chamber effective in curing cough and asthma due to phlegm and retained fluid， which has not received enough attention for a long time because of its simple description. Both action mechanism of this formula and its clinical practical value are revealed by analyzing the mechanism of cough and asthma due to phlegm and retained fluid as well as its clinical manifestation， herbal compatibility and Function characteristics. It is proved that Zeqi Decoction shares the similar function with Shizao Decoction， Kongxian Pill in draining water and removing phlegm. However， apart from mainly efficient in dispelling pathogens like activating yang， relieving retained fluid， lowering the adverse qi to stop cough， Zeqi Decoction is also able to support the healthy qi， making itself suitable to to treat cough and asthma due to phlegm-fluid lying latent in the lung or fluid retained in chest and hypochondrium.
Exploration on Treatment of Children with Immune Thrombocytopenia from Xiaoer Weisheng Zongwei Lunfang
GUOYun-shan, YUHui-ping
2016, 32(5): 416-418.
[Abstract](1002) [PDF 1030KB](613)
Abstract:
As a common hemorrhagic disease in children， immune thrombocytopenia is always treated with the same method as blood trouble by modern doctors via referencing the relevant theory recorded in the ancient literatures. Taking Xiaoer Weisheng Zongwei Lunfang for instance， it has recorded pathogenesis， treatment methods and prescription of blood trouble in children， which provides clinical reference for treating children with immune thrombocytopenia. From the textbook， the pathogenesis of children blood trouble should be heat over-restraining blood and qi. The treatment should focus on clearing away heat， assisted with removing blood stasis and stopping bleeding with astringents. Herbs should be chosen based on their meridian tropisms together with bleeding locations and characteristics of diseased organs. Although partial formulations are seldomly used in the clinical practice， it still provides guidance for application of Chinese herb decoction.
Exploration on Treatment of Children with Immune Thrombocytopenia from Xiaoer Weisheng Zongwei Lunfang
GUOYun-shan, YUHui-ping
2016, 32(5): 416-418.
[Abstract](966) [PDF 380KB](1132)
Abstract:
As a common hemorrhagic disease in children， immune thrombocytopenia is always treated with the same method as blood trouble by modern doctors via referencing the relevant theory recorded in the ancient literatures. Taking Xiaoer Weisheng Zongwei Lunfang for instance， it has recorded pathogenesis， treatment methods and prescription of blood trouble in children， which provides clinical reference for treating children with immune thrombocytopenia. From the textbook， the pathogenesis of children blood trouble should be heat over-restraining blood and qi. The treatment should focus on clearing away heat， assisted with removing blood stasis and stopping bleeding with astringents. Herbs should be chosen based on their meridian tropisms together with bleeding locations and characteristics of diseased organs. Although partial formulations are seldomly used in the clinical practice， it still provides guidance for application of Chinese herb decoction.
Effects of Taohong Siwu Decoction on Inflammatory Factors and Hemorheology in Patients with Femoral Shaft Fracture After Surgery
MAOJin-hua, TUGang-cheng, QIUJing, SUNPing
2016, 32(5): 419-421.
[Abstract](963) [PDF 1027KB](634)
Abstract:
OBJECTIVE To explore the effects of Taohong Siwu Decoction on inflammatory factors and hemorheology in patients with femoral shaft fracture after surgery. METHODS 79 patients with femoral shaft fracture after surgery in the control group were treated with conventional therapy while the equal number of patients in the study group were given Taohong Siwu Decoction. The clinical curative effect， TCM syndrome， interleukin-1(IL-1)， interleukin-6(IL-6)， tumor necrosis factor-α (TNF-α)， whole blood high shear viscosity (ηhigh)， whole blood midst shear viscosity(ηmidst)， whole blood low shear viscosity (ηlow)， plasma viscosity(ηplasm)， hematocrit value(HCT)， white blood cell(WBC) count and sedimentation equation-K value of both groups were compared. RESULTS The total curative effects of study group and control group were 97.02% and 79.75% respectively(P<0.01). Both groups experienced a decrease in pain， swelling， ecchymosis， IL1， IL6， TNFα， ηhigh， ηmidst， ηlow， ηplasm， HCT， WBC and sedimentation equation-K value and the treatment group was more superior to the control group(P<0.05~0.01). CONCULSION Taohong Siwu Decoction can significantly inhibit the inflammatory response and improve hemorheology indexes and clinical symptom for patients with femoral shaft fracture after surgery.
Effects of Taohong Siwu Decoction on Inflammatory Factors and Hemorheology in Patients with Femoral Shaft Fracture After Surgery
MAOJin-hua, TUGang-cheng, QIUJing, SUNPing
2016, 32(5): 419-421.
[Abstract](804) [PDF 325KB](952)
Abstract:
OBJECTIVE To explore the effects of Taohong Siwu Decoction on inflammatory factors and hemorheology in patients with femoral shaft fracture after surgery. METHODS 79 patients with femoral shaft fracture after surgery in the control group were treated with conventional therapy while the equal number of patients in the study group were given Taohong Siwu Decoction. The clinical curative effect， TCM syndrome， interleukin-1(IL-1)， interleukin-6(IL-6)， tumor necrosis factor-α (TNF-α)， whole blood high shear viscosity (ηhigh)， whole blood midst shear viscosity(ηmidst)， whole blood low shear viscosity (ηlow)， plasma viscosity(ηplasm)， hematocrit value(HCT)， white blood cell(WBC) count and sedimentation equation-K value of both groups were compared. RESULTS The total curative effects of study group and control group were 97.02% and 79.75% respectively(P<0.01). Both groups experienced a decrease in pain， swelling， ecchymosis， IL1， IL6， TNFα， ηhigh， ηmidst， ηlow， ηplasm， HCT， WBC and sedimentation equation-K value and the treatment group was more superior to the control group(P<0.05~0.01). CONCULSION Taohong Siwu Decoction can significantly inhibit the inflammatory response and improve hemorheology indexes and clinical symptom for patients with femoral shaft fracture after surgery.
Clinical Study of Anoectochilus Roxburghii Liquid in Treating Oral Ulcer Due to Chemotherapy for Childhood Leukemia
LIQin, HUANGWei, ZHOUWen, LIJian, CHENZai-sheng
2016, 32(5): 422-424.
[Abstract](977) [PDF 1025KB](626)
Abstract:
OBJECTIVE To observe the clinical effects of Anoectochilus Roxburghii liquid in treating oral ulcer due to chemotherapy for childhood leukemia. METHODS 76 children with oral ulcer due to chemotherapy for childhood leukemia were randomly divided into control group(40 cases) treated with Kaijiankou Sprayer and treatment group(36 cases) treated with Anoectochilus Roxburghii liquid. Mouth ulceration and its pain degree of both groups were compared before and after treatment together with the total clinical effects. RESULTS The treatment group possessed evident advantages over the control group in improving oral ulcerative areas(P<0.05). The treatment group was superior in relieving the pain to the control group(P<0.05). And the ulcer classification of the treatment group was better than that of the control group(P<0.05). No evident side effects were detected in the two groups. CONCULSION Anoectochilus Roxburghii liquid has better clinical effects than Kaijiankou sprayer in treating oral ulcer due to chemotherapy for childhood leukemia， which deserves wide clinical application and promotion.
Clinical Study of Anoectochilus Roxburghii Liquid in Treating Oral Ulcer Due to Chemotherapy for Childhood Leukemia
LIQin, HUANGWei, ZHOUWen, LIJian, CHENZai-sheng
2016, 32(5): 422-424.
[Abstract](923) [PDF 338KB](986)
Abstract:
OBJECTIVE To observe the clinical effects of Anoectochilus Roxburghii liquid in treating oral ulcer due to chemotherapy for childhood leukemia. METHODS 76 children with oral ulcer due to chemotherapy for childhood leukemia were randomly divided into control group(40 cases) treated with Kaijiankou Sprayer and treatment group(36 cases) treated with Anoectochilus Roxburghii liquid. Mouth ulceration and its pain degree of both groups were compared before and after treatment together with the total clinical effects. RESULTS The treatment group possessed evident advantages over the control group in improving oral ulcerative areas(P<0.05). The treatment group was superior in relieving the pain to the control group(P<0.05). And the ulcer classification of the treatment group was better than that of the control group(P<0.05). No evident side effects were detected in the two groups. CONCULSION Anoectochilus Roxburghii liquid has better clinical effects than Kaijiankou sprayer in treating oral ulcer due to chemotherapy for childhood leukemia， which deserves wide clinical application and promotion.
Influence of Panax Notoginseng Saponins Combined with Aspirin on Senile Coronary Heart Disease by Thromboela-stogram
YANG Qing-feng, CUI De-zhi, YAN Xiao-yan
2016, 32(5): 425-427.
[Abstract](1062) [PDF 1070KB](728)
Abstract:
OBJECTIVE To observe antithrombotic effects of panax notoginseng saponins plus aspirin via applying thromboela-stogram(TEG). METHODS 51 patients with senile coronary heart disease were randomly divided into treatment group and control group， with patients in the control group being given aspirin while whose in the treatment group being treated with Sanqi Tongshu Capsule and aspirin. TEG of both groups were observed together with platelet inhibition rate(AA%) induced by arachidonic acid. There existed a statistical significance between the two groups in improving R value， K value and alpha Angle(P<0.05~0.01). RESULTS Reducing scores on SDRS and HAMA of the treatment group before and after treatment were evidently higher than those of the control group(P<0.01). Both groups were effective in improving PSG parameters including sleeping time， sleep latency and sleep efficiency after treatment(P<0.05)， but the treatment group was superior to the control group(P<0.05). The total curative rate of treatment group and control group were 84.85% and 67.74% respectively with a statistical significance between the two groups(P<0.05). CONCULSION Panax notoginseng saponins plus aspirin is superior to aspirin in anti-thrombosis， without increasing the side effects.
Influence of Panax Notoginseng Saponins Combined with Aspirin on Senile Coronary Heart Disease by Thromboela-stogram
YANG Qing-feng, CUI De-zhi, YAN Xiao-yan
2016, 32(5): 425-427.
[Abstract](1057) [PDF 384KB](1094)
Abstract:
OBJECTIVE To observe antithrombotic effects of panax notoginseng saponins plus aspirin via applying thromboela-stogram(TEG). METHODS 51 patients with senile coronary heart disease were randomly divided into treatment group and control group， with patients in the control group being given aspirin while whose in the treatment group being treated with Sanqi Tongshu Capsule and aspirin. TEG of both groups were observed together with platelet inhibition rate(AA%) induced by arachidonic acid. There existed a statistical significance between the two groups in improving R value， K value and alpha Angle(P<0.05~0.01). RESULTS Reducing scores on SDRS and HAMA of the treatment group before and after treatment were evidently higher than those of the control group(P<0.01). Both groups were effective in improving PSG parameters including sleeping time， sleep latency and sleep efficiency after treatment(P<0.05)， but the treatment group was superior to the control group(P<0.05). The total curative rate of treatment group and control group were 84.85% and 67.74% respectively with a statistical significance between the two groups(P<0.05). CONCULSION Panax notoginseng saponins plus aspirin is superior to aspirin in anti-thrombosis， without increasing the side effects.
Influence of Acupuncture Combined with Rehabilitation Training on Neurological Function and Athletic Ability in Patients with Apoplexy Sequela
FAN Xue-ke, GUO Lin
2016, 32(5): 428-430.
[Abstract](880) [PDF 1031KB](982)
Abstract:
OBJECTIVE To explore the influence of acupuncture combined with rehabilitation training on neurological function and athletic ability in patients with apoplexy sequela. METHODS 134 cases of apoplexy sequela were randomly divided into the study group and control group， with 67 cases in each group. Patients in the study group were given acupuncture combined with rehabilitation training， while those in the control group were given pure acupuncture， with the treatment course lasting for 8 weeks. Then changes of the neurological function， activity of daily living(ADL) and athletic ability of the affected extremity were compared between the two groups to evaluate the curative effects. RESULTS NIHSS scores of both groups went down notably， whereas Barthel index and Fugl-Meyer scores went up evidently， with a statistical significance when compared to the situation before treatment(P<0.05~0.01). The changes of the study group was more evident， with a statistical significance when compared to the control group(P<0.05). The total curative rate of the study group was 92.54%(62/67)， which was superior to 71.64% (48/67) of the control group with a statistical significance(P<0.01). CONCULSION Acupuncture combined with rehabilitation training is able to improve both neurological function and athletic ability in patients with apoplexy sequela.
Influence of Acupuncture Combined with Rehabilitation Training on Neurological Function and Athletic Ability in Patients with Apoplexy Sequela
FAN Xue-ke, GUO Lin
2016, 32(5): 428-430.
[Abstract](878) [PDF 345KB](927)
Abstract:
OBJECTIVE To explore the influence of acupuncture combined with rehabilitation training on neurological function and athletic ability in patients with apoplexy sequela. METHODS 134 cases of apoplexy sequela were randomly divided into the study group and control group， with 67 cases in each group. Patients in the study group were given acupuncture combined with rehabilitation training， while those in the control group were given pure acupuncture， with the treatment course lasting for 8 weeks. Then changes of the neurological function， activity of daily living(ADL) and athletic ability of the affected extremity were compared between the two groups to evaluate the curative effects. RESULTS NIHSS scores of both groups went down notably， whereas Barthel index and Fugl-Meyer scores went up evidently， with a statistical significance when compared to the situation before treatment(P<0.05~0.01). The changes of the study group was more evident， with a statistical significance when compared to the control group(P<0.05). The total curative rate of the study group was 92.54%(62/67)， which was superior to 71.64% (48/67) of the control group with a statistical significance(P<0.01). CONCULSION Acupuncture combined with rehabilitation training is able to improve both neurological function and athletic ability in patients with apoplexy sequela.
Clinical Research of Shensong Yangxin Capsule Combined with Rhodiola Rosea on Radioactive Heart Damage
SHEN Wei-sheng, XIA De-hong, GAO Chun-heng, XI Lei, CAO Xiang-ming, DENG Li-chun, SHENG Hua-ming, ZENG Jie
2016, 32(5): 431-434.
[Abstract](899) [PDF 1048KB](1170)
Abstract:
OBJECTIVE To explore the cardiac protective effects of Shensong Yangxin Capsule on patients with chest tumor radiotherapy. METHODS 120 patients with thoracic tumors(including lung cancer， breast cancer， mediastinal tumor and esophageal cancer) requiring radiotherapy were randomly divided into radiotherapy combined with Shensong Yangxin Capsule group (group A)， radiotherapy with Rhodiola rosea group(group B)， radiotherapy plus Shensong Yangxin Capsule and Rhodiola rosea group(group C) and radiotherapy alone group (group D)， with 30 patients in each group. Levels of serum transforming growth factor(TGF-β1)， tumor necrosis factor(TNF-α)， endothelin(ET) contents， occurrence rate of radioactive heart damage of each group were compared within three months after radiotherapy and changes of myocardial integrated back scatter(IBS) in the third month were observed. RESULTS After radiotherapy the contents of TGF-β1， TNF-α and ET contents of group A B and C were lower than those of the group D (P<0.01). The incidence rates of acute cardiac injury of the first three groups were also lower than those of the group D within one to three months after radiotherapy(P<0.05). IBS levels of anterior wall and anterior inter-ventricular septum in the third month after radiotherapy were increased significantly in the group D while no changes were detected in the other three groups.CONCULSION Shensong Yangxin Capsule combined with Rhodiola rosea is able to reduce the production of inflammatory cytokines in tissues and inhibit the increase of IBS during radiotherapy so as to protect heart from radiation injury.
Clinical Research of Shensong Yangxin Capsule Combined with Rhodiola Rosea on Radioactive Heart Damage
SHEN Wei-sheng, XIA De-hong, GAO Chun-heng, XI Lei, CAO Xiang-ming, DENG Li-chun, SHENG Hua-ming, ZENG Jie
2016, 32(5): 431-434.
[Abstract](864) [PDF 400KB](964)
Abstract:
OBJECTIVE To explore the cardiac protective effects of Shensong Yangxin Capsule on patients with chest tumor radiotherapy. METHODS 120 patients with thoracic tumors(including lung cancer， breast cancer， mediastinal tumor and esophageal cancer) requiring radiotherapy were randomly divided into radiotherapy combined with Shensong Yangxin Capsule group (group A)， radiotherapy with Rhodiola rosea group(group B)， radiotherapy plus Shensong Yangxin Capsule and Rhodiola rosea group(group C) and radiotherapy alone group (group D)， with 30 patients in each group. Levels of serum transforming growth factor(TGF-β1)， tumor necrosis factor(TNF-α)， endothelin(ET) contents， occurrence rate of radioactive heart damage of each group were compared within three months after radiotherapy and changes of myocardial integrated back scatter(IBS) in the third month were observed. RESULTS After radiotherapy the contents of TGF-β1， TNF-α and ET contents of group A B and C were lower than those of the group D (P<0.01). The incidence rates of acute cardiac injury of the first three groups were also lower than those of the group D within one to three months after radiotherapy(P<0.05). IBS levels of anterior wall and anterior inter-ventricular septum in the third month after radiotherapy were increased significantly in the group D while no changes were detected in the other three groups.CONCULSION Shensong Yangxin Capsule combined with Rhodiola rosea is able to reduce the production of inflammatory cytokines in tissues and inhibit the increase of IBS during radiotherapy so as to protect heart from radiation injury.
Clinical Observation of Therapeutic Effects of Strong Acupuncture Stimulation to the Second Cervical Vertebra Transverse Process on Cervical Spondylosis of Vertebral Artery Type
GEWei, HOULi-hao, ZHANGHai-li, OUYANGGang, ZHAOZheng-rui
2016, 32(5): 435-438.
[Abstract](981) [PDF 1047KB](1031)
Abstract:
OBJECTIVE To observe the therapeutic effect of cervical spondylosis of vertebral artery type(CSA) with strong acupuncture stimulation to the second cervical vertebra transverse process. METHODS 100 patients with CSA were randomly divided into 3 groups. 35 cases in the treatment group were treated with strong acupuncture stimulation to the second cervical vertebra transverse process for 10 minutes per day. 30 cases in the control group were treated with routine acupuncture treatment for 30 minutes per day. 35 cases in the hybrid group were first treated with strong acupuncture stimulation to the second cervical vertebra transverse process， which was followed by routine acupuncture treatment. RESULTS After 4 weeks of treatment， the total effective rate of the hybrid group was 94.1%， with the treatment group being 85.7% and the control group being 73.3%. And notable statistical differences were detected among the three groups， with the hybrid group being superior to the treatment group and the treatment group being superior to the control group(P<0.05). Both hybrid group and treatment group were better than control group in onset time(P<0.05)， with no evident difference being noticed between the hybrid group and treatment group(P>0.05). The treatment time of the hybrid group was shorten than that of the treatment group(P<0.05)， together with the treatment group being shorten than the control group(P<0.05). Both hybrid group and treatment group were superior to control group in blood flow volume and average blood flow velocity(P<0.05)， with no evident difference being noticed between the hybrid group and treatment group(P>0.05). CONCULSION The strong acupuncture stimulation to the second cervical vertebra transverse process has immediate effects in relieving symptoms of CSA and is able to be shorten the treatment course by improving blood supply of vertebral artery.
Clinical Observation of Therapeutic Effects of Strong Acupuncture Stimulation to the Second Cervical Vertebra Transverse Process on Cervical Spondylosis of Vertebral Artery Type
GEWei, HOULi-hao, ZHANGHai-li, OUYANGGang, ZHAOZheng-rui
2016, 32(5): 435-438.
[Abstract](1055) [PDF 384KB](930)
Abstract:
OBJECTIVE To observe the therapeutic effect of cervical spondylosis of vertebral artery type(CSA) with strong acupuncture stimulation to the second cervical vertebra transverse process. METHODS 100 patients with CSA were randomly divided into 3 groups. 35 cases in the treatment group were treated with strong acupuncture stimulation to the second cervical vertebra transverse process for 10 minutes per day. 30 cases in the control group were treated with routine acupuncture treatment for 30 minutes per day. 35 cases in the hybrid group were first treated with strong acupuncture stimulation to the second cervical vertebra transverse process， which was followed by routine acupuncture treatment. RESULTS After 4 weeks of treatment， the total effective rate of the hybrid group was 94.1%， with the treatment group being 85.7% and the control group being 73.3%. And notable statistical differences were detected among the three groups， with the hybrid group being superior to the treatment group and the treatment group being superior to the control group(P<0.05). Both hybrid group and treatment group were better than control group in onset time(P<0.05)， with no evident difference being noticed between the hybrid group and treatment group(P>0.05). The treatment time of the hybrid group was shorten than that of the treatment group(P<0.05)， together with the treatment group being shorten than the control group(P<0.05). Both hybrid group and treatment group were superior to control group in blood flow volume and average blood flow velocity(P<0.05)， with no evident difference being noticed between the hybrid group and treatment group(P>0.05). CONCULSION The strong acupuncture stimulation to the second cervical vertebra transverse process has immediate effects in relieving symptoms of CSA and is able to be shorten the treatment course by improving blood supply of vertebral artery.
Effect of Paeoniflorin on Hippocamp Tissue Pathomorphology and BDNF Level of Forced Swimming Rats Model
XUE Mei, MU Dao-zhou, HUANG Xi
2016, 32(5): 439-441.
[Abstract](926) [PDF 1273KB](1255)
Abstract:
OBJECTIVE To observe the antidepressant effect of Paeoniflorin and the possible mechanism in acute depressed rat model of forced swimming. METHODS SD rats were randomly divided into control group， model group， peony glycoside group and fluoxetine group（10/group）. The model group， Paeoniflorin group and Fluoxetine group were forced to swim for 15min to establish the depressed model. Treatment groups were administered paeoniflorin(10mg/kg) and fluoxetine (20mg/kg)， respectively， 3 times in 24h. Control group were treated with vehicle(saline). Observation indexes: the opening experiment of behavior， levels of BDNF in serum and hippocampus and the pathological observation of hippocampus. RESULTS Paeoniflorin significantly increased the motility distance of rats(P<0.01) and BDNF levels in rat serum and hippocampus(P<0.01)， which displayed protective effects on hippocampus pathomorphology. CONCLUSION Paeoniflorin shows antidepressant effect in forced swimming animal model， which probably relates to the BDNF/TrkB receptor signal transduction pathway.
Effect of Paeoniflorin on Hippocamp Tissue Pathomorphology and BDNF Level of Forced Swimming Rats Model
XUE Mei, MU Dao-zhou, HUANG Xi
2016, 32(5): 439-441.
[Abstract](868) [PDF 602KB](957)
Abstract:
OBJECTIVE To observe the antidepressant effect of Paeoniflorin and the possible mechanism in acute depressed rat model of forced swimming. METHODS SD rats were randomly divided into control group， model group， peony glycoside group and fluoxetine group（10/group）. The model group， Paeoniflorin group and Fluoxetine group were forced to swim for 15 min to establish the depressed model. Treatment groups were administered paeoniflorin(10 mg/kg) and fluoxetine (20 mg/kg)， respectively， 3 times in 24 h. Control group were treated with vehicle(saline). Observation indexes: the opening experiment of behavior， levels of BDNF in serum and hippocampus and the pathological observation of hippocampus. RESULTS Paeoniflorin significantly increased the motility distance of rats(P<0.01) and BDNF levels in rat serum and hippocampus(P<0.01)， which displayed protective effects on hippocampus pathomorphology. CONCLUSION Paeoniflorin shows antidepressant effect in forced swimming animal model， which probably relates to the BDNF/TrkB receptor signal transduction pathway.
Effects of Ilex Pubescens Total Flavonoids on Improving Cerebral Ischemic Tolerence
FANG Xiao-yan, ZUO Ting, QIAO Jing-yi, FENG Su-xiang, MIAO Ming-san
2016, 32(5): 442-446.
[Abstract](950) [PDF 1481KB](1114)
Abstract:
OBJECTIVE To observe the neuroprotective effects of ilex pubescens total flavonoids(IPTF) on the rats with cerebral ischemic preconditioning(CIP) and the expressions of BDNF， GDNF and VEGF in the cortex and hippocampus CA1 area， to explore the mechanisms of IPTF on improving cerebral ischemic tolerance. METHODS CIP was performed by bilateral common carotid artery occlusion for 10min in rats. 72h later， cerebral ischemia reperfusion(I/R) was established by middle cerebral artery occlusion(MCAO) for 2h. Wistar rats were randomly devided into five groups， Sham group， I/R group， CIP+MCAO group， IPTF high dose group（200mg/kg） and IPTF low dose group（100mg/kg）。The behavioral injury was detected by neurologic deficit scores(NDS)， and the infarct areas were detected by TTC staining. The expressions of BDNF， GDNF and VEGF in cortex and hippocampus CA1 area were measured by immunohistochemical staining. RESULTS IPTF decreased the neurologic deficit scores and the infarct areas. It also increased the positive area and integral optical density of BDNF， VEGF in cortex and CA1 area of rats with CIP. CONCLUSION The mechanisms of IPTF on improving cerebral ischemic tolerance may be related with the up-regulation of the endogenous protein BDNF and VEGF.
Effects of Ilex Pubescens Total Flavonoids on Improving Cerebral Ischemic Tolerence
FANG Xiao-yan, ZUO Ting, QIAO Jing-yi, FENG Su-xiang, MIAO Ming-san
2016, 32(5): 442-446.
[Abstract](861) [PDF 812KB](1162)
Abstract:
OBJECTIVE To observe the neuroprotective effects of ilex pubescens total flavonoids(IPTF) on the rats with cerebral ischemic preconditioning(CIP) and the expressions of BDNF， GDNF and VEGF in the cortex and hippocampus CA1 area， to explore the mechanisms of IPTF on improving cerebral ischemic tolerance. METHODS CIP was performed by bilateral common carotid artery occlusion for 10 min in rats. 72 h later， cerebral ischemia reperfusion(I/R) was established by middle cerebral artery occlusion(MCAO) for 2 h. Wistar rats were randomly devided into five groups， Sham group， I/R group， CIP+MCAO group， IPTF high dose group（200 mg/kg） and IPTF low dose group（100 mg/kg）。The behavioral injury was detected by neurologic deficit scores(NDS)， and the infarct areas were detected by TTC staining. The expressions of BDNF， GDNF and VEGF in cortex and hippocampus CA1 area were measured by immunohistochemical staining. RESULTS IPTF decreased the neurologic deficit scores and the infarct areas. It also increased the positive area and integral optical density of BDNF， VEGF in cortex and CA1 area of rats with CIP. CONCLUSION The mechanisms of IPTF on improving cerebral ischemic tolerance may be related with the up-regulation of the endogenous protein BDNF and VEGF.
Effect of Baicalin on Expression of NF-κB in Ulcerative Colitis Rats
ZHULei, SHENHong, GUPei-qing, JIANGYin, LIUYa-jun, ZHANGLu, CHENGJia-fei
2016, 32(5): 447-450.
[Abstract](1001) [PDF 1485KB](697)
Abstract:
OBJECTIVE To explore the effect of Baicalin in vivo of the expression of NF-κB and cytokines in ulcerative colitis rats. METHODS The colitis model of mice were established with TNBS methord.Mice were randomly divided into control group，model group，Baicalin group，and SASP group.After continuous administration for 10 days，observed the colon gross morphological changes，the colon tissue level of TNF-α，IL-8 and IL-1 were detected using ELISA， and the protein expression of NF-κB in the colon tissues of mice were detected using Western Blot. RESULTS Compared with the model group，Baicalin(100mg/kg) group could significantly improve the colon gross morphological form，the Baicalin(25，50，100mg/kg) groups could inhibit the secretion of inflammatory factors dose-dependently， the high dose group could reduce IL-1，IL-8 and TNF-αexpression (P<0.05)， no significant difference occured between the SASP group. Compared with the model group，.Baicalin high dose group could reduce NF-kB levels， promote IkBα level (P<0.05) . CONCLUSION Baicalin can inhibit NF-κB activation， and thus play its anti-inflammatory effect in ulcerative colitis mice.
Effect of Baicalin on Expression of NF-κB in Ulcerative Colitis Rats
ZHULei, SHENHong, GUPei-qing, JIANGYin, LIUYa-jun, ZHANGLu, CHENGJia-fei
2016, 32(5): 447-450.
[Abstract](843) [PDF 833KB](1304)
Abstract:
OBJECTIVE To explore the effect of Baicalin in vivo of the expression of NF-κB and cytokines in ulcerative colitis rats. METHODS The colitis model of mice were established with TNBS methord.Mice were randomly divided into control group，model group，Baicalin group，and SASP group.After continuous administration for 10 days，observed the colon gross morphological changes，the colon tissue level of TNF-α，IL-8 and IL-1 were detected using ELISA， and the protein expression of NF-κB in the colon tissues of mice were detected using Western Blot. RESULTS Compared with the model group，Baicalin(100 mg/kg) group could significantly improve the colon gross morphological form，the Baicalin(25，50，100 mg/kg) groups could inhibit the secretion of inflammatory factors dose-dependently， the high dose group could reduce IL-1，IL-8 and TNF-αexpression (P<0.05)， no significant difference occured between the SASP group. Compared with the model group，.Baicalin high dose group could reduce NF-kB levels， promote IkBα level (P<0.05) . CONCLUSION Baicalin can inhibit NF-κB activation， and thus play its anti-inflammatory effect in ulcerative colitis mice.
Study on Alisols Hypolipidemic Effect and Molecular Mechanism
XUFei, YUHui, LUCai, WUQi-nan, GUWei, CHENJun
2016, 32(5): 451-455.
[Abstract](1016) [PDF 1131KB](764)
Abstract:
OBJECTIVE To study the different effects and molecular mechanism of Alisol acetates(alisol B 23-acetate and alisol A 24-acetate)on the decrease of the hyperlipidemia of fat mice. METHODS Established a hyperlipidemic mice model，measured the level of TC， TG and HDL-C after the administration of Alisol acetates，detected the activity of LCAT， which is a key enzyme in lipid metabolism，obtained the molecular structure of LCAT by homology modeling and studied the interaction between LCAT and Alisol acetates using molecular modeling. RESULTS Alisol acetates played a hypolipidemic effect by reducing TG， TC level and increasing HDL-C level. lisol acetates decreased the LCAT activity. Alisol acetates binding regions are in the range of Leu201~Phe305. CONCLUSION Alisol acetates elevated HDL-C mechanism may not be by modulating LCAT activity. The region of Leu201~Phe305 may be the suppression positions. Ile227， Arg217， Gly229 may be the critical amino acid residues for the interaction.
Study on Alisols Hypolipidemic Effect and Molecular Mechanism
XUFei, YUHui, LUCai, WUQi-nan, GUWei, CHENJun
2016, 32(5): 451-455.
[Abstract](941) [PDF 457KB](1132)
Abstract:
OBJECTIVE To study the different effects and molecular mechanism of Alisol acetates(alisol B 23-acetate and alisol A 24-acetate)on the decrease of the hyperlipidemia of fat mice. METHODS Established a hyperlipidemic mice model，measured the level of TC， TG and HDL-C after the administration of Alisol acetates，detected the activity of LCAT， which is a key enzyme in lipid metabolism，obtained the molecular structure of LCAT by homology modeling and studied the interaction between LCAT and Alisol acetates using molecular modeling. RESULTS Alisol acetates played a hypolipidemic effect by reducing TG， TC level and increasing HDL-C level. lisol acetates decreased the LCAT activity. Alisol acetates binding regions are in the range of Leu201~Phe305. CONCLUSION Alisol acetates elevated HDL-C mechanism may not be by modulating LCAT activity. The region of Leu201~Phe305 may be the suppression positions. Ile227， Arg217， Gly229 may be the critical amino acid residues for the interaction.
Study on Protective Effects of Salidrosides on Pancreatic β-Cell Survival
JULin-jie, WENXiao-hua, SHULuan
2016, 32(5): 456-460.
[Abstract](1023) [PDF 1285KB](646)
Abstract:
OBJECTIVE To investigate the hypoglycemic action and β-cell protective effect of salidroside in streptozotocin（STZ） induced diabetic mice and cultured mouse islets. METHODS C57BL/6J mice were injected with a single dose of 150mg/kg freshly prepared STZ with citrate buffer as control. The salidroside intervention with a dosage of 100mg/kg/d was initiated on the 8th day after STZ injection and conducted for 30d. Fasting blood glucose levels were measured every five days. After 30d treatment， the oral glucose tolerance test(OGTT) was performed， and blood samples were collected to detect plasma insulin concentrations. The isolated mouse islets were cultured with salidroside(50μmol/L) or DMSO for 3d. Ki67 staining and TUNEL assay were performed to investigate the effects of salidroside on β-cell proliferation and apoptosis. Meanwhile， the mRNA levels of insulin，Pdx-1，GLP-1R and IL-1β in islets were detected by RT-PCR. RESULTS Compared with the STZ group， salidroside displayed significantly hypoglycemic effects， together with increased plasma insulin contents as well as improved OGTT. The Ki67 staining in cultured islets showed the proliferation of β-cell was remarkably increased by salidroside， while the β-cell apoptosis induced by high glucose was strongly inhibited by salidroside. Moreover， the mRNA levels of insulin， Pdx-1 and GLP-1R were up-regulated by salidroside significantly. However， the mRNA level of IL-1β which is a cytokine involved in β-cell apoptosis was down-regulated by salidroside. CONCLUSION The present study demonstrates that salidroside can ameliorate the hyperglycemia in STZ diabetic mice by protecting β-cell survival with increased β-cell proliferation and decreased β-cell apoptosis.
Study on Protective Effects of Salidrosides on Pancreatic β-Cell Survival
JULin-jie, WENXiao-hua, SHULuan
2016, 32(5): 456-460.
[Abstract](971) [PDF 642KB](1007)
Abstract:
OBJECTIVE To investigate the hypoglycemic action and β-cell protective effect of salidroside in streptozotocin（STZ） induced diabetic mice and cultured mouse islets. METHODS C57BL/6J mice were injected with a single dose of 150 mg/kg freshly prepared STZ with citrate buffer as control. The salidroside intervention with a dosage of 100 mg/kg/d was initiated on the 8th day after STZ injection and conducted for 30 d. Fasting blood glucose levels were measured every five days. After 30 d treatment， the oral glucose tolerance test(OGTT) was performed， and blood samples were collected to detect plasma insulin concentrations. The isolated mouse islets were cultured with salidroside(50 μmol/L) or DMSO for 3 d. Ki67 staining and TUNEL assay were performed to investigate the effects of salidroside on β-cell proliferation and apoptosis. Meanwhile， the mRNA levels of insulin，Pdx-1，GLP-1R and IL-1β in islets were detected by RT-PCR. RESULTS Compared with the STZ group， salidroside displayed significantly hypoglycemic effects， together with increased plasma insulin contents as well as improved OGTT. The Ki67 staining in cultured islets showed the proliferation of β-cell was remarkably increased by salidroside， while the β-cell apoptosis induced by high glucose was strongly inhibited by salidroside. Moreover， the mRNA levels of insulin， Pdx-1 and GLP-1R were up-regulated by salidroside significantly. However， the mRNA level of IL-1β which is a cytokine involved in β-cell apoptosis was down-regulated by salidroside. CONCLUSION The present study demonstrates that salidroside can ameliorate the hyperglycemia in STZ diabetic mice by protecting β-cell survival with increased β-cell proliferation and decreased β-cell apoptosis.
Effects of Grain-Moxibustion on Signaling Molecule of Hydrogen Sulfide in Adriamycin-Induced Cardiomyopathy Rats
XIAOYan, DINGLiang, CHENHao, GUYi-huang
2016, 32(5): 461-464.
[Abstract](947) [PDF 1151KB](668)
Abstract:
OBJECTIVE To observe the inhibitory effect of grain-moxibustion on ADR-induced cardiotoxicity， and explore the possible mechanism from the perspective of endogenous hydrogen sulfide (H2S). METHODS 30 SPF-grade SD rats were randomly divided into blank control group， model group and grain-moxibustion group， 10 cases in each one. Rats in the model group and the grain-moxibustion group were treated with tail vein of ADR to establish the ADR-induced cardiotoxicity models. Rats in the blank group were treated with an equal amount of saline. Rats in the grain-moxibustion group were treated with grain-moxibustion at a same time of dosing building every day at Guanyuan(CV4) and Juque(CV14)， 5 cones at each acupoint， once daily for 4 weeks，2d of rest per week. At the end of the last intervention， we detected HR， LVSP)， LVDP， +dp/dtmax， -dp/dtmax and t-dp/dtmax. Serum were collected to test the activities of LDH and CK and the content of cTnI， and myocardium tissue were collected to measure the content of H2S， to make a comprehensive assessment of the cardiac function in rats. Myocardial tissue biopsies were prepared to observe the morphological changes of myocardial pathology organization. RESULTS Compared with the blank group and grain-moxibustion group， the HR， LVSP， +dp/dtmax and -dp/dtmax of the model group were reduced， while LVDP and t-dp/dtmax were increased（P<0.05）. Compared with the blank group， the activity of LDH， CK and the content of cTnI of the model group and the grain-moxibustion group were increased(P<0.05)， the content of H2S in myocardium was reduced (P<0.05). Compared with the model group， the activity of LDH， CK and the content of cTnI of the grain-moxibustion group were reduced(P<0.05)， the content of H2S in myocardium was increased (P<0.05). In the blank group， structure of myocardial cells is complete， intercellular space was normal without myocardial cells damage. While swelling， hyperemia and disordered arrangement of myocardial cells were observed by optical microscopy in the model group. But the situation of swelling and hyperemia in the grain-moxibustion group were milder than that in the model group. CONCLUSION Grain-moxibustion can improve the abnormal cardiac function and alleviate myocardial cells swelling and hyperemia which caused by ADR. The mechanism may be related with the regulation of the generation of endogenous H2S.
Effects of Grain-Moxibustion on Signaling Molecule of Hydrogen Sulfide in Adriamycin-Induced Cardiomyopathy Rats
XIAOYan, DINGLiang, CHENHao, GUYi-huang
2016, 32(5): 461-464.
[Abstract](916) [PDF 517KB](1059)
Abstract:
OBJECTIVE To observe the inhibitory effect of grain-moxibustion on ADR-induced cardiotoxicity， and explore the possible mechanism from the perspective of endogenous hydrogen sulfide (H2S). METHODS 30 SPF-grade SD rats were randomly divided into blank control group， model group and grain-moxibustion group， 10 cases in each one. Rats in the model group and the grain-moxibustion group were treated with tail vein of ADR to establish the ADR-induced cardiotoxicity models. Rats in the blank group were treated with an equal amount of saline. Rats in the grain-moxibustion group were treated with grain-moxibustion at a same time of dosing building every day at Guanyuan(CV4) and Juque(CV14)， 5 cones at each acupoint， once daily for 4 weeks，2 d of rest per week. At the end of the last intervention， we detected HR， LVSP)， LVDP， +dp/dtmax， -dp/dtmax and t-dp/dtmax. Serum were collected to test the activities of LDH and CK and the content of cTnI， and myocardium tissue were collected to measure the content of H2S， to make a comprehensive assessment of the cardiac function in rats. Myocardial tissue biopsies were prepared to observe the morphological changes of myocardial pathology organization. RESULTS Compared with the blank group and grain-moxibustion group， the HR， LVSP， +dp/dtmax and -dp/dtmax of the model group were reduced， while LVDP and t-dp/dtmax were increased（P<0.05）. Compared with the blank group， the activity of LDH， CK and the content of cTnI of the model group and the grain-moxibustion group were increased(P<0.05)， the content of H2S in myocardium was reduced (P<0.05). Compared with the model group， the activity of LDH， CK and the content of cTnI of the grain-moxibustion group were reduced(P<0.05)， the content of H2S in myocardium was increased (P<0.05). In the blank group， structure of myocardial cells is complete， intercellular space was normal without myocardial cells damage. While swelling， hyperemia and disordered arrangement of myocardial cells were observed by optical microscopy in the model group. But the situation of swelling and hyperemia in the grain-moxibustion group were milder than that in the model group. CONCLUSION Grain-moxibustion can improve the abnormal cardiac function and alleviate myocardial cells swelling and hyperemia which caused by ADR. The mechanism may be related with the regulation of the generation of endogenous H2S.
Screening on Toxicity of 26 Kinds of Common Orthopedic Herbal Medicine Using Zebrafish Model
CHENYing, WANGJing, CHENShu-qin, JINGLi-jun, WEIYing-jie
2016, 32(5): 465-469.
[Abstract](925) [PDF 1375KB](661)
Abstract:
OBJECTIVE Toxicity model of zebrafish was used to evaluate safety of 26 kinds of common orthopedic herbal medicine. METHODS Zebrafish embryo at 1 day post fertilization (dpf) were exposed with various concentration of water decoction; the death number of the embryos or larvals was counted from 1dpf to 6 dpf; embryonic micro-morphology of zebrafish (3dpf) was observed and pictures were taken that compared with 0.4% DMSO; LC50 value at 6dpf was calculated by SPSS16.0. RESULTS The results indicated that 13 kinds of water decoction of herbal medicines (Dipsaci Radix， Anemarrhena Rhizoma， Olibanum and Myrrha， Psoraleae Fructus， Angelicae Pubescentis Radix， Achyranthis Bidentatae Radix， Polygoni multiflori Radix， Cnidii Fructus， Aucklandiae Radix， Eupolyphaga Steleophaga， Epimedii Folium and Acanthopanacis Cortex) can cause obvious organ toxicity to juvenile zebrafish， such as yolk sac swelling， deformation， black， pericardial edema and bleeding， and their LC50 values were low (16.9~689.7μg/mL)， while the other 13 herbal medicines are relatively safe (Pyrolae Herba， Homalomena Rhizoma， Sappan Lignum， Morindae Officinalis Radix， Testudinis Carapax Et Plastrum， Drynariae Rhizoma， Salviae miltiorrhizae Radix Et Rhizoma， Cibotii Rhizoma， Taxilli Herba， spatholobi CauLis， Rehmanniae Radix Praeparata， Ligustri Lucidi Fructus and Eucommiae Cortex)， no fish organ toxicity was observed， and their concentration caused zebrafish death was usually at high levels (above 2000μg/mL). CONCLUSION Zebrafish model was successfully used for screening toxicity of 26 common orthopedic herbal medicine， which may provide useful information for reasonable application of them. Zebrafish model has advantages of simplicity， high efficiency and real-time observation， which made large scale toxicity screening of herbal medicine in vivo style possible.
Screening on Toxicity of 26 Kinds of Common Orthopedic Herbal Medicine Using Zebrafish Model
CHENYing, WANGJing, CHENShu-qin, JINGLi-jun, WEIYing-jie
2016, 32(5): 465-469.
[Abstract](1064) [PDF 732KB](1113)
Abstract:
OBJECTIVE Toxicity model of zebrafish was used to evaluate safety of 26 kinds of common orthopedic herbal medicine. METHODS Zebrafish embryo at 1 day post fertilization (dpf) were exposed with various concentration of water decoction; the death number of the embryos or larvals was counted from 1dpf to 6 dpf; embryonic micro-morphology of zebrafish (3dpf) was observed and pictures were taken that compared with 0.4% DMSO; LC50 value at 6dpf was calculated by SPSS16.0. RESULTS The results indicated that 13 kinds of water decoction of herbal medicines (Dipsaci Radix， Anemarrhena Rhizoma， Olibanum and Myrrha， Psoraleae Fructus， Angelicae Pubescentis Radix， Achyranthis Bidentatae Radix， Polygoni multiflori Radix， Cnidii Fructus， Aucklandiae Radix， Eupolyphaga Steleophaga， Epimedii Folium and Acanthopanacis Cortex) can cause obvious organ toxicity to juvenile zebrafish， such as yolk sac swelling， deformation， black， pericardial edema and bleeding， and their LC50 values were low (16.9~689.7 μg/mL)， while the other 13 herbal medicines are relatively safe (Pyrolae Herba， Homalomena Rhizoma， Sappan Lignum， Morindae Officinalis Radix， Testudinis Carapax Et Plastrum， Drynariae Rhizoma， Salviae miltiorrhizae Radix Et Rhizoma， Cibotii Rhizoma， Taxilli Herba， spatholobi CauLis， Rehmanniae Radix Praeparata， Ligustri Lucidi Fructus and Eucommiae Cortex)， no fish organ toxicity was observed， and their concentration caused zebrafish death was usually at high levels (above 2 000 μg/mL). CONCLUSION Zebrafish model was successfully used for screening toxicity of 26 common orthopedic herbal medicine， which may provide useful information for reasonable application of them. Zebrafish model has advantages of simplicity， high efficiency and real-time observation， which made large scale toxicity screening of herbal medicine in vivo style possible.
Multi-Layer Analysis of Chloriti Lapis on Rats Model with COPD
YANGWen-guo, WANGRui, LIUSheng-jin, WUDe-kang, YUBin, FANGFang, YANGJiang
2016, 32(5): 470-474.
[Abstract](976) [PDF 1402KB](602)
Abstract:
OBJECTIVE To study the curative effects of Chloriti Lapis on rats with chronic obstructive pulmonary diseases(COPD). METHODS Rats models of COPD were established and were given the powder， decoction and residues of Chloriti Lapis of both high dosage and low dosage respectively based on its clinical application methods and dosage. Inflammatory factor content in rats lung tissue and blood serum was tested and relative indexes were evaluated through canonical correlation analysis， principal component analysis and multiple linear statistical models. RESULTS The high dosage group of decoction reached the best result， with the high dosage group of powder being the second and the high dosage group of residues being the third. CONCULSION Chloriti Lapis is effective in reducing inflammatory factors of both blood serum and lung tissue of rats models with COPD. The curative effect differences due to processing methods can be detected by canonical correlation analysis， principal component analysis and multiple linear statistical models.
Multi-Layer Analysis of Chloriti Lapis on Rats Model with COPD
YANGWen-guo, WANGRui, LIUSheng-jin, WUDe-kang, YUBin, FANGFang, YANGJiang
2016, 32(5): 470-474.
[Abstract](868) [PDF 751KB](954)
Abstract:
OBJECTIVE To study the curative effects of Chloriti Lapis on rats with chronic obstructive pulmonary diseases(COPD). METHODS Rats models of COPD were established and were given the powder， decoction and residues of Chloriti Lapis of both high dosage and low dosage respectively based on its clinical application methods and dosage. Inflammatory factor content in rats lung tissue and blood serum was tested and relative indexes were evaluated through canonical correlation analysis， principal component analysis and multiple linear statistical models. RESULTS The high dosage group of decoction reached the best result， with the high dosage group of powder being the second and the high dosage group of residues being the third. CONCULSION Chloriti Lapis is effective in reducing inflammatory factors of both blood serum and lung tissue of rats models with COPD. The curative effect differences due to processing methods can be detected by canonical correlation analysis， principal component analysis and multiple linear statistical models.
Study on the Excretion of Liguzinediol in Rats by UPLCPDA Method
DONG Bang, YOU Xiaoqin, WEN Hongmei, DAI Zhenli, SHAN Chenxiao, WANG Xinzhi, LI Wei
2016, 32(5): 475-478.
[Abstract](941) [PDF 1240KB](639)
Abstract:
OBJECTIVE To study the excretion of Liguzinediol and its metabolites in rats by UPLCPDA method. METHODS Six SD rats were administered intravenously at a dose of 10mg/kg. Its urine， bile and feces were collected according to certain time points. The samples were treated by methanol and the supernatant was dried by N2， then dissolved by mobile phase. Liguzinediol and its metabolites in urine， bile and feces were determinated by UPLCPDA. Conversion factor was confirmed through the determination of UV absorption coefficient and molecular weight of prototype drug together with its metabolites. Then the cumulative excretion of Liguzinediol in rats(Dose%) was calculated. RESULTS There were slight differences in the excretion process between female and male rats. The Dose% of Liguzinediol and its main metabolites in female rats urine， bile and feces respectively were 47.94%， 16.67% and 0.648%， and the total Dose% was 65.26%. The Dose% of Liguzinediol and its main metabolites in male rats urine， bile and feces respectively were 35.00%， 20.37% and 1.156%， and the total Dose% was 56.53%. CONCLUSION The UPLCPDA method with the addition of conversion factor can be used to explore the material balance of Liguzinediol in rats， and provide experimental evidence for clinical research.
Study on the Excretion of Liguzinediol in Rats by UPLCPDA Method
DONG Bang, YOU Xiaoqin, WEN Hongmei, DAI Zhenli, SHAN Chenxiao, WANG Xinzhi, LI Wei
2016, 32(5): 475-478.
[Abstract](930) [PDF 552KB](1072)
Abstract:
OBJECTIVE To study the excretion of Liguzinediol and its metabolites in rats by UPLCPDA method. METHODS Six SD rats were administered intravenously at a dose of 10 mg/kg. Its urine， bile and feces were collected according to certain time points. The samples were treated by methanol and the supernatant was dried by N2， then dissolved by mobile phase. Liguzinediol and its metabolites in urine， bile and feces were determinated by UPLCPDA. Conversion factor was confirmed through the determination of UV absorption coefficient and molecular weight of prototype drug together with its metabolites. Then the cumulative excretion of Liguzinediol in rats(Dose%) was calculated. RESULTS There were slight differences in the excretion process between female and male rats. The Dose% of Liguzinediol and its main metabolites in female rats urine， bile and feces respectively were 47.94%， 16.67% and 0.648%， and the total Dose% was 65.26%. The Dose% of Liguzinediol and its main metabolites in male rats urine， bile and feces respectively were 35.00%， 20.37% and 1.156%， and the total Dose% was 56.53%. CONCLUSION The UPLCPDA method with the addition of conversion factor can be used to explore the material balance of Liguzinediol in rats， and provide experimental evidence for clinical research.
Pharmacokinetic Study of Scutebarbatine A and Scutebarbatine B in Rats After Orally Administration of Scutellaria Barbata Extract Using a LC-MS/MS Method
GUOYue-long, GUOJin-rui, ZHANGLei
2016, 32(5): 479-482.
[Abstract](1020) [PDF 1195KB](635)
Abstract:
OBJECTIVE The aim of this study was to develop and validate a LC-MS/MS method to simultaneously determination of Scutebarbatine A and Scutebarbatine B in rat plasma and applicated this method to pharmacokinetic study of Scutebarbatine A and Scutebarbatine B after oral administration of Scutellaria barbata extract. METHODS Plasma samples were prepared with ethyl acetate using liquid-liquid extraction. Then samples were separated by using BDS Hypersil C18(50mm×2.1mm， 2.4μm) with gradient elution program and oven temperature was set at 40℃. And the mobile phase was consisted with acetonitrile/water (0.05% formic acid) at a flow rate of 0.25mL/min. Determination was carried out on a tandem mass spectrometer in positive ion mode using a multiple reaction monitoring (MRM) via a electrospray ionization (ESI) interface. After oral administration of 500mg/kg Scutellaria barbata extract， plasma samples were collected and analyzed by using a DAS software to analyze to the pharmacokinetic parameters. RESULTS Scutebarbatine A and Scutebarbatine B were liner at the range of 0.512~258.0ng/mL， 0.482~241.0ng/mL， respectively with a r2 larger than 0.994. Accuracy， precious， extraction efficiency， matrix effects and stability study were all meet the requirement of the bioanalytical method. The AUC(0-t) for Scutebarbatine A and Scutebarbatine B were (88.69±12.4)μg/L·h， (57.09±9.84)μg/L·h， respectively. And the Cmax for Scutebarbatine A and Scutebarbatine B were (10.22±1.31)ng/mL and (6.27±0.80)ng/mL. CONCLUSION The method was simple， accurate， and sensitive， which could be used for the pharmacokinetic study of Scutebarbatine A and Scutebarbatine B after oral administration of Scutellaria barbata extract.
Pharmacokinetic Study of Scutebarbatine A and Scutebarbatine B in Rats After Orally Administration of Scutellaria Barbata Extract Using a LC-MS/MS Method
GUOYue-long, GUOJin-rui, ZHANGLei
2016, 32(5): 479-482.
[Abstract](1179) [PDF 513KB](1132)
Abstract:
OBJECTIVE The aim of this study was to develop and validate a LC-MS/MS method to simultaneously determination of Scutebarbatine A and Scutebarbatine B in rat plasma and applicated this method to pharmacokinetic study of Scutebarbatine A and Scutebarbatine B after oral administration of Scutellaria barbata extract. METHODS Plasma samples were prepared with ethyl acetate using liquid-liquid extraction. Then samples were separated by using BDS Hypersil C18(50 mm×2.1 mm， 2.4 μm) with gradient elution program and oven temperature was set at 40 ℃. And the mobile phase was consisted with acetonitrile/water (0.05% formic acid) at a flow rate of 0.25 mL/min. Determination was carried out on a tandem mass spectrometer in positive ion mode using a multiple reaction monitoring (MRM) via a electrospray ionization (ESI) interface. After oral administration of 500 mg/kg Scutellaria barbata extract， plasma samples were collected and analyzed by using a DAS software to analyze to the pharmacokinetic parameters. RESULTS Scutebarbatine A and Scutebarbatine B were liner at the range of 0.512~258.0 ng/mL， 0.482~241.0 ng/mL， respectively with a r2 larger than 0.994. Accuracy， precious， extraction efficiency， matrix effects and stability study were all meet the requirement of the bioanalytical method. The AUC(0-t) for Scutebarbatine A and Scutebarbatine B were (88.69±12.4)μg/L·h， (57.09±9.84)μg/L·h， respectively. And the Cmax for Scutebarbatine A and Scutebarbatine B were (10.22±1.31)ng/mL and (6.27±0.80)ng/mL. CONCLUSION The method was simple， accurate， and sensitive， which could be used for the pharmacokinetic study of Scutebarbatine A and Scutebarbatine B after oral administration of Scutellaria barbata extract.
Metabonomics Research on the Urine of Model of Dampness Stagnancy due to Spleen Deficiency Based on Liquid Mass Spectrometry
YANG Bin-bin, JI Xu-ming, WANG Shi-jun
2016, 32(5): 483-486.
[Abstract](905) [PDF 1215KB](871)
Abstract:
OBJECTIVE In metabonomics method based on liquid mass combination of dampness stagnancy due to spleen deficiency model and normal control group rats urine， To explore the differences metabolites in the urine of the model rats. METHODS The model of spleen deficiency and dampness stagnancy in rats was induced with high-fat and low-protein diet plus exhaustive swimming methods. Using high performance liquid chromatography(HPLC)-quadrupole-time of flight tandem mass spectrometer technique to detect the small molecule metabolites in urine samples in the 2 groups， Metabolic fingerprint as principal component analysis and partial least squares discriminant analysis， find out the differences of characteristics of metabolites. RESULTS Compared with the control group， the urine of rats in model group identified 6 different metabolites， respectively: alpha keto glutarate， citrulline， lactic acid， glutamine， citric acid， serotonin. CONCLUSION Dampness stagnancy due to spleen deficiency model rats body sugar and protein metabolism in disorder， energy supply， gastrointestinal function drops， antioxidant ability， its mechanism may be related to the change of the small molecule metabolites.
Metabonomics Research on the Urine of Model of Dampness Stagnancy due to Spleen Deficiency Based on Liquid Mass Spectrometry
YANG Bin-bin, JI Xu-ming, WANG Shi-jun
2016, 32(5): 483-486.
[Abstract](952) [PDF 565KB](992)
Abstract:
OBJECTIVE In metabonomics method based on liquid mass combination of dampness stagnancy due to spleen deficiency model and normal control group rats urine， To explore the differences metabolites in the urine of the model rats. METHODS The model of spleen deficiency and dampness stagnancy in rats was induced with high-fat and low-protein diet plus exhaustive swimming methods. Using high performance liquid chromatography(HPLC)-quadrupole-time of flight tandem mass spectrometer technique to detect the small molecule metabolites in urine samples in the 2 groups， Metabolic fingerprint as principal component analysis and partial least squares discriminant analysis， find out the differences of characteristics of metabolites. RESULTS Compared with the control group， the urine of rats in model group identified 6 different metabolites， respectively: alpha keto glutarate， citrulline， lactic acid， glutamine， citric acid， serotonin. CONCLUSION Dampness stagnancy due to spleen deficiency model rats body sugar and protein metabolism in disorder， energy supply， gastrointestinal function drops， antioxidant ability， its mechanism may be related to the change of the small molecule metabolites.
Formulationandprocess Optimization of Puerarin-loaded PEG-PLGA Nanoparticles Modified by Hyaluronic Acid and Its in Vitro Evaluation
XUE Rui, SHEN Xiao-hua, YANG Jie, LI Qian
2016, 32(5): 487-490.
[Abstract](977) [PDF 1181KB](738)
Abstract:
OBJECTIVE With PEG-PLGA as the carrier， and the preparation of hyaluronic acid modified kudzu root element PEG-PLGA nanoparticles by nanoprecipitation method， to optimize the preparation prescription， and preliminary evaluation of its properties in vitro. METHODS With PEG PLGA as the carrier， Hyaluronic acid as surface modification agent， preparation of hyaluronic acid modified kudzu root element PEG-PLGA HA/Pue-NPs by nanoprecipitation method: design and optimize the prescription by Orthogonal experimental， characterize its in vitro properties， examine the in vitro drug release behavior of HA/Pue-NPs. RESULTS Prepared drug-loading nanoparticles has the spherical appearance. The average particle size and Zeta potential are (88.9±2.2 )nm、（-21.9± 0.54）mV respectively， drug loadings and the coating rate are 6.75%， 78.52%. In vitro drug release test shows that the drug-loading nanoparticles release slowly， the accumulative release rate is 65.8% within 24h. CONCLUSION The particle size of HA/Pue-NPs is consistent， good in vitro properties and certain slow-release characterized.
Formulationandprocess Optimization of Puerarin-loaded PEG-PLGA Nanoparticles Modified by Hyaluronic Acid and Its in Vitro Evaluation
XUE Rui, SHEN Xiao-hua, YANG Jie, LI Qian
2016, 32(5): 487-490.
[Abstract](1053) [PDF 501KB](972)
Abstract:
OBJECTIVE With PEG-PLGA as the carrier， and the preparation of hyaluronic acid modified kudzu root element PEG-PLGA nanoparticles by nanoprecipitation method， to optimize the preparation prescription， and preliminary evaluation of its properties in vitro. METHODS With PEG PLGA as the carrier， Hyaluronic acid as surface modification agent， preparation of hyaluronic acid modified kudzu root element PEG-PLGA HA/Pue-NPs by nanoprecipitation method: design and optimize the prescription by Orthogonal experimental， characterize its in vitro properties， examine the in vitro drug release behavior of HA/Pue-NPs. RESULTS Prepared drug-loading nanoparticles has the spherical appearance. The average particle size and Zeta potential are (88.9±2.2 )nm、（-21.9± 0.54）mV respectively， drug loadings and the coating rate are 6.75%， 78.52%. In vitro drug release test shows that the drug-loading nanoparticles release slowly， the accumulative release rate is 65.8% within 24h. CONCLUSION The particle size of HA/Pue-NPs is consistent， good in vitro properties and certain slow-release characterized.
Preparation Optimization of Baicalein Nano-micelles by Box-Behnken Design-Response Method
FUDong-ning, LIXiang-yong, CHANGZhi-hui, XULian-ming
2016, 32(5): 491-494.
[Abstract](1109) [PDF 1412KB](721)
Abstract:
OBJECTIVE To prepare the Nanomicelles of Baicalein-polyethylene glycol-12 hydroxy stearic acid ester-phospholipids for improving its solubility， and to optimize its technology. METHODS Baicalein-solutol HS15- phospholipid (BA-Sol-Pls) were prepared by film-dispersion method. Box-behnken design was employed， with the amount of ethanol (X1)， concentration of solutol (X2) and phospholipids (X3) as the investigation object. Particle size analyzer was used to investigate the particle size and Zeta potential of Nanomicelles. The encapsulation efficiency and drug loading of micelles were determined by Ultracentrifugation. RESULTS The BA-Sol-Pls prepared by optimal formulation were well-distributed. The average particle diameter was (410±5.98) nm， Zeta potential was -(21±0.92) mV. The encapsulation efficiency was 90.38% and the drug-loading rate was 5.35%. CONCLUSION The box-behnken design was effective and suitable for optimization the preparation technology of baicalein nano micelles.
Preparation Optimization of Baicalein Nano-micelles by Box-Behnken Design-Response Method
FUDong-ning, LIXiang-yong, CHANGZhi-hui, XULian-ming
2016, 32(5): 491-494.
[Abstract](903) [PDF 716KB](926)
Abstract:
OBJECTIVE To prepare the Nanomicelles of Baicalein-polyethylene glycol-12 hydroxy stearic acid ester-phospholipids for improving its solubility， and to optimize its technology. METHODS Baicalein-solutol HS15- phospholipid (BA-Sol-Pls) were prepared by film-dispersion method. Box-behnken design was employed， with the amount of ethanol (X1)， concentration of solutol (X2) and phospholipids (X3) as the investigation object. Particle size analyzer was used to investigate the particle size and Zeta potential of Nanomicelles. The encapsulation efficiency and drug loading of micelles were determined by Ultracentrifugation. RESULTS The BA-Sol-Pls prepared by optimal formulation were well-distributed. The average particle diameter was (410±5.98) nm， Zeta potential was -(21±0.92) mV. The encapsulation efficiency was 90.38% and the drug-loading rate was 5.35%. CONCLUSION The box-behnken design was effective and suitable for optimization the preparation technology of baicalein nano micelles.
Clinical Effects of Bailemian Capsule Combined with Deanxit in Treating 40 Cases of Insomnia Due to Anxiety
WANG Qi, ZHANG Xiao-lin, ZHU Ying, QIN Da-qiang, CHEN Hui-juan, YU Ming
2016, 32(5): 495-497.
[Abstract](1092) [PDF 1033KB](840)
Abstract:
OBJECTIVE To observe the clinical effects of Bailemian Capsule combined with deanxit in treating patients with insomnia due to anxiety. METHODS 80 patients with insomnia due to anxiety were included and randomly divided into treatment group and control group， with 40 cases in each group. Patients in the treatment group were given Bailemian Capsule combined with deanxit， while whose in the control group were treated with deanxit only， with the treatment course lasting for 4 weeks. No other antianxiety agents， antipsychotics or sedative hypnotics were applied during the study. Patients who dropped out were eliminated from the analysis. Hamilton anxiety scale(HAMA)， sleep dysfunction rating scale(SDRS) and PSG parameters were applied to evaluate the curative effects. RESULTS Reducing scores on SDRS and HAMA of the treatment group before and after treatment were evidently higher than those of the control group(P<0.05). Both groups were effective in improving PSG parameters including sleeping time， sleep latency and sleep efficiency after treatment(P<0.05)， but the treatment group was superior to the control group(P<0.05). The total curative rate of treatment group and control group were 84.85% and 61.29% respectively， with a statistical significance between the two groups(P<0.05). CONCULSION Bailemian Capsule combined with deanxit is able to notably improve insomnia due to anxiety without increasing the side effects， which is batter than deanxit intake only， deserving further clinical application and promotion.
Clinical Effects of Bailemian Capsule Combined with Deanxit in Treating 40 Cases of Insomnia Due to Anxiety
WANG Qi, ZHANG Xiao-lin, ZHU Ying, QIN Da-qiang, CHEN Hui-juan, YU Ming
2016, 32(5): 495-497.
[Abstract](922) [PDF 369KB](1134)
Abstract:
OBJECTIVE To observe the clinical effects of Bailemian Capsule combined with deanxit in treating patients with insomnia due to anxiety. METHODS 80 patients with insomnia due to anxiety were included and randomly divided into treatment group and control group， with 40 cases in each group. Patients in the treatment group were given Bailemian Capsule combined with deanxit， while whose in the control group were treated with deanxit only， with the treatment course lasting for 4 weeks. No other antianxiety agents， antipsychotics or sedative hypnotics were applied during the study. Patients who dropped out were eliminated from the analysis. Hamilton anxiety scale(HAMA)， sleep dysfunction rating scale(SDRS) and PSG parameters were applied to evaluate the curative effects. RESULTS Reducing scores on SDRS and HAMA of the treatment group before and after treatment were evidently higher than those of the control group(P<0.05). Both groups were effective in improving PSG parameters including sleeping time， sleep latency and sleep efficiency after treatment(P<0.05)， but the treatment group was superior to the control group(P<0.05). The total curative rate of treatment group and control group were 84.85% and 61.29% respectively， with a statistical significance between the two groups(P<0.05). CONCULSION Bailemian Capsule combined with deanxit is able to notably improve insomnia due to anxiety without increasing the side effects， which is batter than deanxit intake only， deserving further clinical application and promotion.
Application of Quantitative Analysis Multi-Components by Single Marker in Xiaozheng capsule Quality Control
LIJin, ZHAOXiao-li
2016, 32(5): 498-500.
[Abstract](983) [PDF 1045KB](617)
Abstract:
OBJECTIVE To establish a method of quantitative analysis on multi-components by single marker (QAMS) to determine 5 kinds of anthraquinones in Xiaozheng capsule. METHODS Using emodin as internal referring substance，to establish the relative correction factor (fk/s) of Aloe Emodin， rhein， chrysophanol， physcion to emodin and determine these contents. At the same time， an external standard method was established to determine the amounts of 5 kinds of anthraquinones， then compared with the QAMS method. RESULTS The two methods did not show significant difference in assay results according to the data in a certain linear range. CONCLUSION The QAMS method is feasible and credible， and can be used to determine and control the 5 kinds of anthraquinones in Xiaozheng capsule.
Application of Quantitative Analysis Multi-Components by Single Marker in Xiaozheng capsule Quality Control
LIJin, ZHAOXiao-li
2016, 32(5): 498-500.
[Abstract](852) [PDF 334KB](1121)
Abstract:
OBJECTIVE To establish a method of quantitative analysis on multi-components by single marker (QAMS) to determine 5 kinds of anthraquinones in Xiaozheng capsule. METHODS Using emodin as internal referring substance，to establish the relative correction factor (fk/s) of Aloe Emodin， rhein， chrysophanol， physcion to emodin and determine these contents. At the same time， an external standard method was established to determine the amounts of 5 kinds of anthraquinones， then compared with the QAMS method. RESULTS The two methods did not show significant difference in assay results according to the data in a certain linear range. CONCLUSION The QAMS method is feasible and credible， and can be used to determine and control the 5 kinds of anthraquinones in Xiaozheng capsule.

Copyright © Journal of Nanjing University of traditional Chinese Medicine

Sponsored by：Nanjing University of traditional Chinese MedicineAddress：No.138 xianlin Avenue, Qixia District, Nanjing cityChina Pos：210023

Tel：025-85811935Email：xb@njucm.edu.cn

Supported by: Beijing Renhe Information Technology Co., Ltd.