南京中医药大学学报

Discussion on the Application of Cancerous Toxin Pathogenesis Theory in the Treatment of Gastric Cancer

LUWei, SHENZheng-jie, CHENGHai-bo

2016, 32(2): 101-103.

[Abstract](716) [PDF 994KB](641)

Abstract:
China has a very high incidence of gastric cancer. Chinese medicine has advantages in the treating of gastric cancer. Professor Zhou Zhong-yin， awarded National Chinese Medical Science Master， has put forward the theory of cancerous toxin pathogenesis theory based on his long-term clinical experience. Better effect of the treatment of gastric cancer has been confirmed by the application of cancerous toxin pathogenesis theory on gastric cancer patients. Cancerous toxin remaining in the stomach is the key pathogenesis of gastric cancer. Cancerous toxin always intermingled with phlegm turbidity， blood stasis， damp turbidity and toxic heat is very common in the patients with gastric cancer. So the principle and treatment of gastric cancer is the combination of anti-cancer while removing toxic material and other therapeutic principle， such as regulating qi and harmonizing stomach， clearing heat and promoting diuresis， promoting blood circulation to remove blood stasis， reducing phlegm and resolving masses and reinforcing deficiency.

Study on Disease Location of Nausea and Vomiting

SUN Peng-cheng, WU Cheng-yu

2016, 32(2): 104-106.

[Abstract](669) [PDF 1003KB](775)

Abstract:
Being common symptoms in clinic， both nausea and vomiting are the characteristic reflection of failure of descending of stomach-qi from Diagnostics of Chinese Medicine. This paper probes into the view of "vomiting location being spleen rather than stomach"， based on the record in Miraculous Pivot that such symptoms as nausea and vomiting only belong to diseases of spleen meridian of foot-taiyin. Furthermore， a theory that "vomiting due to dysfunction of five viscera will lead to discomfort of stomach" is put forward， according to the relevant discussion in Huangdi's Canon of Internal Medicine and Treatise on Febrile Diseases. By analyzing the pathogenesis， treatment and prescription of vomiting due to dysfunction of five viscera， treatment rules that "stomach regulation can't be neglected though vomiting location isn't in stomach" is summarized， revealing that Chinese medicine emphasizes more of syndrome differentiation than symptom differentiation.

Analysis of the Mechanism of Treating Cough Variant Asthma with Wind-expelling Drugs

WANG Yue-jiao, MA Jian

2016, 32(2): 107-110.

[Abstract](697) [PDF 1025KB](760)

Abstract:
Cough Variant Asthma (CVA) is one of the common causes of chronic cough. Recurrent irritating tussiculation is CVA's main clinical manifestation. Modern medicine uses drugs which treat bronchial asthma to control the disease or to relieve symptoms. It is stated that pathogenic-wind-invading-lung is the main factor accounting for the forming of CVA in traditional Chinese medicine. Treating CVA with Chinese herbal medicine such as wind-expelling drugs has achieved certain effects. Based on comprehensive analysis of characteristics of wind-expelling drugs， it is reported that wind-expelling drugs can dispel wind pathogen， rise Spleen Qi introduce drugs into meridians， relieve cough and relieve spasm. The study can provide theoretical basis and feasible methods in clinical practice to treat CVA.

Clinical Research on the Inhibition of Recurrence and Metastasis of Triple Negative Breast Cancer with the Method of Detoxification and Blood Stasis Dissipating

LIU Min, WANG Ming-wu

2016, 32(2): 111-113.

[Abstract](731) [PDF 1010KB](712)

Abstract:
OBJECTIVE To observe the clinical efficacy of method of detoxification and blood stasis dissipating on the inhibition of recurrence and metastasis of triple negative breast cancer (TNBC). METHODS 40 cases of TNBC patients after operation were randomly divided into the control group and the treatment group， with 20 cases in each group. The control group was treated according to syndrome differentiation. The treatment group was treated with the method of detoxification and blood stasis dissipating beside the syndrome differentiation. Observe the disease free survival (DFS) of patients after 3-year treatment. RESULTS The DFS in the treatment group after 3-year treatment was 100%， while the control group 75% (P<0.05). CONCLUSION The method of detoxification and blood stasis dissipating can effectively inhibit the recurrence and metastasis of triple negative breast cancer.

Clinical Study of Modified Shaofu Zhuyu Decoction in Treating Adenomyosis Dysmenorrheal

FENG Hui, ZANG Li, ZHANG De-zhong

2016, 32(2): 114-117.

[Abstract](636) [PDF 1033KB](691)

Abstract:
OBJECTIVE To study the curative effects and action mechanism of using Shaofu Zhuyu decoction to treat adenomyosis dysmenorrhea. METHODS From June 2014 to June 2015 in our hospital 80 cases of patients with uterine adenomyosis dysmenorrhea were chosen and randomly divided into the observation group and the control group (40 cases in each group). The observation group had Shaofu Zhuyu decoction 5~7 d before dysmenorrhea， while the control group had Yueyue shu decoction， 1 bag/time. The two groups all had treatment of three menstrual cycles. Dysmenorrhea symptoms before and after treatment and the changes of TCM symptoms as well as the serum of CA125， TNF-α and IL-8 levels were recorded and compared， and ultrasound measurement was used to calculate the size of the uterus before and after the treatment. RESULTS In the two groups after treatment， dysmenorrhea scores and TCM symptoms all declined significantly (P<0.01)， but that of the observation group decreased more obviously (P<0.05). And after 3 courses of treatment in the observation group， the total effective rate was 95%， significantly higher than 67.5% in the control group; And TCM symptom in the observation group was 95% higher than 70% in the control group (P<0.05). Compared with the condition before treatment， uterine volume had no significantly changes differences after treatment (P>0.05). After treatment， uterine volume of the observation group compared with the control group had no significant differences (P>0.05). In the two groups after treatment CA125， TNF-α， IL-8 all declined significantly (P<0.05~0.01)， but that of the observation group decreased more obviously (P<0.05~0.01). During the treatment， heart， liver and kidney were all within the normal range， without obvious adverse reactions. CONCLUSION Using Shaofu Zhuyu Decoction to treat adenomyosis dysmenorrhea could significantly alleviate symptoms of patients with dysmenorrhea， relieving TCM symptoms. And its mechanism may be related to the lowering of the serum CA125， TNF-α， IL-8 levels.

Clinical Research of Gushibao Pill on Severe Kidney Circulation Deficiency and Blood Stasis Type Knee Osteoarthritis

REN Wei-liang, ZHU Yan-feng, HAN Kun, YU Jian, HU Zhi-yong, LIU Shi-cheng, WANG Jin-bang, TIAN Ya-feng, QI Li-qing

2016, 32(2): 118-121.

[Abstract](660) [PDF 1026KB](651)

Abstract:
OBJECTIVE To observe the clinical effect and safety of treatment of Gushibao pill on severe kidney circulation deficiency and blood stasis type knee osteoarthritis. METHODS 70 patients with severe knee osteoarthritis were selected for this study， and were divided into the treatment group (38 cases) and the control group (32 cases). Patients of two groups were given health education and basic knee stick therapy treatment. On the basis of basic treatment， patients in the control group were given glucosamine hydrochloride capsules orally and treatment group were give Gubaoshi pill. WOMAC Osteoarthritis Index Scale and ISOA knee osteoarthritis severity index scale were used to evaluate the outcomes of two groups. Chinese medicine symptom score were used to evaluate the clinical symptoms. ESR， calcitonin (CT) and bone alkaline phosphatase (BALP) were detected before and after treatment. RESULTS Erythrocyte sedimentation rate in the control group significantly decreased after a course of treatment (P<0.05)， and bone alkaline phosphatase and calcitonin showed no significant change(P>0.05). Erythrocyte sedimentation rate decreased significantly in treatment group after treatment (P<0.05)， and bone alkaline phosphatase and calcitonin showed no significant change (P>0.05). Scored of symptoms and signs significantly decreased in two groups， and the decreased extent of treatment group was significantly greater than that of the control group (P<0.05). Pain score， WOMAC score and ISOA scores were significantly lower in two groups of patients compared with the condition before treatment (P<0.05). After treatment， pain score， WOMAC score and ISOA scores of treatment group were lower than those of the control group (P<0.05). The total effective rate of treatment group was 97.37%， and was significantly higher than the control group (80.00%). The difference was statistically significant (P<0.05). Two patients had no obvious adverse reactions during treatment. CONCLUSION Gushibao pill can decrease the level of erythrocyte sedimentation rate in patients with severe kidney circulation deficiency and blood stasis knee osteoarthritis. It is effective and safe.

Clinical Observation of Influence of Yuquanwan Decoction on Inflammatory Cytokines in Type 2 Diabetic Kidney Disease

PENG Cong, GAO Ming-song, KONG Cai-xia, GUO Wei, KE Shu-hong, ZHOU Yang

2016, 32(2): 122-124.

[Abstract](663) [PDF 1006KB](647)

Abstract:
OBJECTIVE To investigate the influence of Yuquanwan decoction on inflammatory cytokines in type 2 diabetic kidney disease(DKD). METHODS 80 cases with Type 2 diabetic kidney disease were randomly divided into two groups: the control group(40 cases) and the treatment group(40 cases). The control group was given benazepril hydrochloride tablets with 10mg per day. And health education， diet control and physical exercises were also performed. Besides the above ways of treatment， the treatment group was given Yuquanwan decoction. Changes of mA1b， 24 hUmTP， HbA1c， TNF-α and IL-1 were observed before and after the treatment. RESULTS mA1b， 24 hUmTP， HbA1c， TNF-α and IL-1 in the treatment group showed significant decrease(P<0.01) and the difference between the two groups had significant statistical significance(P<0.05~0.01). CONCLUSION Yuquanwan decoction can decrease inflammatory cytokines， thus protecting kidney function in DKD.

The Influence of Shenmai Injection Combined with Chemotherapy on T cell Subgroups and Cytokines of Advanced Non-small Cell Lung Cancer Patients

WANG Rong, LI Da-peng, FENG Jun

2016, 32(2): 125-128.

[Abstract](614) [PDF 1021KB](630)

Abstract:
OBJECTIVE To observe the impact of Shenmai injection combined with chemotherapy on advanced non-small cell lung cancer patients' immunity and cytokines. METHODS 48 cases were randomly divided into two groups: the combining treatment group and the chemotherapy group. Patients with squamous carcinoma were given Docetaxel and cisplatin chemotherapy; patients with adenocarcinomas and large cell carcinoma were given pemetrexed disodium together with cisplatin chemotherapy. Shenmai injection was added in the combining treatment group. Immune indexes like peripheral T lymphocyte subsets and the viability of NK cells together with peripheral blood cell factor level and short term effects of chemotherapy and the side effects shown were recorded before the chemotherapy and two cycles of treatment. RESULTS After treatment，the level of CD4⁺ and CD4⁺/CD8⁺ in combined treatment group increased significantly(P<0.05~0.01). Compared to the chemotherapy group， the levels of CD3⁺， CD4⁺ and CD4⁺/CD8⁺ of treatment group have significant difference(P<0.01). The expression of IFN-γ， TNF-α， IL-2 in combination group increased significantly(P<0.01). The levels of IFN-γ and IL-2 of treatment group had significant difference(P<0.01) ，compared with the control group. The response rate of these two groups is similar.The incidence of hematologic toxicities in the treatment group was lower than that in control group(P<0.05~0.01). CONCLUSION The combination of Shenmai injection with chemotherapy can improve advanced stage non-small lung cancer patients' immunity and reverse the Th1/Th2 ratio， and reduce the toxicity of chemotherapy.

Effect of Fuzheng Xiaozhi Diet on the Comprehensive Functions of Stroke Patients with Hemiplegia

WANGAi-hong, XUGui-hua, LIUWei, WUQiu-di, LIUKou-ying

2016, 32(2): 129-133.

[Abstract](642) [PDF 1054KB](661)

Abstract:
OBJECTIVE To explore the effect of Fuzheng Xiaozhi Diet (FXD) on comprehensives function of stroke patients with hemiplegia. METHODS 120 patients were randomly divided into two groups: the treatment group(60 cases) and the control group(60 cases). At the end of the study， there were 58 patients in the treatment group and 50 in the control group. Conventional treatment and care were given to both groups while Fuzheng Xiaozhi diet was added in the treatment group. Functional Comprehensive Assessment were used to assess the comprehensive function(Motor function and cognitive function) and the blood lipid level at T0， the end of three month's treatment(T1)， the end of six month's treatment(T2)， and the end of twelve month's treatment(T3). RESULTS There were significant differences in the main effects of intervention， the main effects of time on scores of motor，comprehensive functions，HDL and TC (P<0.05~0.01）， and there were significant differences in the main effects of time on scores of cognitive function and Blood lipid various indicators (P<0.05~0.01) between two groups， respectively. The scores of motor and comprehensive functions and in experiment group were higher than that in control group 3， 6 and 12 months after intervention (P<0.05~0.01). The scores of cognitive function in experiment group were higher than that in control group 6 and 12 months after intervention (P<0.05). CONCLUSION FXD can significantly improve the motor function， the cognitive function，the comprehensive functions and Lipid levels in stroke patients with hemiplegia.

Clinical Effect Observation of Superficial Needling Therapy on the Breast Tenderness Caused by Mammary Glands Hyperplasia

CHENDong, XIAYou-bing, LINGLi-jun, FUZhong-hua, XIAOAn-ju, LYUKai-lu

2016, 32(2): 134-137.

[Abstract](752) [PDF 1027KB](897)

Abstract:
OBJECTIVE To observe the differences of curative effect among superficial needle therapy， Xiakucao oral solution and Vitamin E soft capsules on the breast tenderness caused by mammary glands hyperplasia and to explore the optimum therapeutic method. METHODS 105 patients with mammary glands hyperplasia were randomly divided into the superficial needling group， the Xiakucao group and the Vitamin E group， with 35 cases in every group. Patients in the superficial needling group were treated with the superficial needling retaining for 5 hours. Patients in the Xiakucao group and the Vitamin E group took medicine with oral administration. Treatment in the three groups all began from 7±3 days before menstruation and stopped during menstruation. The superficial needling group was treated once every other day with total 3 times. Patients in the Xiakucao group took medicine twice a day in the morning and evening. The Vitamin E group was treated once a day. Compare the scores of short form McGill pain Questionnaire (SF-MPQ) before and after the treatment and after one month follow-up. Record the time needed for pain relieving in the first treatment of the superficial needling group and the adverse effects in the three groups. RESULTS After the treatment， the breast tenderness was improved obviously in the three groups (P<0.01) and the superficial needling group was superior to the other groups (P<0.01). During the one-month follow up， the superficial needling group showed good effects (P<0.01). It also showed fast effect on relieving the pain in the first treatment. The Xiakucao group was superior to the Vitamin E group in the improvement of the breast tenderness (P<0.05). CONCLUSION The superficial needling therapy shows fast and lasting effects on relieving the pain of mammary glands hyperplasia， which is superior to Xiakucao oral solution and Vitamin E soft capsules.

Clinical Observation of the Activating Qi Acupunture Therapy on 33 cases of Sciatica Caused by Lumbar Intervertebral Disc Herniation in Middle-Aged and Elderly People

LI Sheng-wu, DAI Guo-gang, QIN Xue-fei, WU Jia

2016, 32(2): 138-141.

[Abstract](694) [PDF 1021KB](700)

Abstract:
OBJECTIVE To observe the clinical efficacy of the activating qi acupuncture therapy on middle-aged and elderly people with sciatica caused by lumbar intervertebral disc herniation. METHODS 65 cases were randomly divided into the control group (the electric acupuncture group) and the treatment group (the activating qi acupuncture group)， with 32 cases and 33 cases respectively in the two groups. Dong's Linggu and Dabai extra points were applied in the treatment with the method of activating qi every 5 minutes and retaining for 30 minutes. In the control group， Ashi points， Jiaji points， Dachangshu and Huantiao were applied as the main points and matching points were applied according to viscera syndrome differentiation and channels and collaterals. Connect the electro-acupuncture apparatus after getting the sensation of qi and select dilatational wave at 50/100Hz retaining for 30 minutes. The two groups were both treated once a day with 2 courses (5 times a course). RESULTS The total effective rate in the treatment group was 90.90%， while the control group 84.38%. The treatment group was superior to the control group in the improvement of visual analogue scale (VAS) of leg pain and lumbago， scores of Roland-Morris Disability Questionnaire (RMQ) (P<0.05) as well as the total efficacy (P<0.05). CONCLUSION The activating qi acupuncture therapy shows obvious effects on the sciatica caused by lumbar intervertebral disc herniation in middle-aged and elderly people， which is recommended in clinical application.

Research of Compatible Ratio of Kai-Xin-San on Regulation of Neurotrophic Factor System in Cortex and Hippocampus on Chronic Unpredictable Mild Stress Induced Depressive Mice

DUAN Xiu-zhu, DUAN Jin-ao, ZHU Yue, CHENG Xiao-xuan, CHEN Xiao-nan, XIAO Jun-yuan, LIU Pei, SU Shu-lan, ZHANG Liu

2016, 32(2): 142-147.

[Abstract](791) [PDF 1223KB](740)

Abstract:
OBJECTIVE To explore the anti-depressive effect of Kai-Xin-San (KXS) with different compatible ratio on chronic unpredictable mild stress (CUMS) treated mouse via neurotrophic factor regulation. METHODS Depressive animal model was created by applying a series of stress procedures on mice and the depressive mice were treated with KXS with different compatible ratio. Several parameters， including sucrose consumption， forced swimming time， were applied for evaluation of the depressive effect of KXS. Afterwards， the mice were sacrificed. The cerebral cortex and hippocampus were took out for the analysis of quantity of neurotrophic factors and the expressions of neurotrophic factor receptors via ELISA， qPCR and western blot analysis. RESULTS KXS with different compatible ratio exerted significant anti-depressive effect on CUMS treated depressive mouse model in presence of increased sucrose consumption， swimming time and open field scores. The amount of neurotrophic factors， NGF and BDNF， and the expression of the corresponding receptors， were both up-regulated after the treatment of KXS. One ratio named D-652 exerted the best effect. CONCLUSION Neurotrophic factor regulation might be one of important active targets for anti-depression effect of KXS.

Regulatory Effect of Bushen Antai Recipe on the Secretion Function and HLA-G Expression of Human Extravillous Cytotrophoblasts HTR-8

QIDan, TANYong

2016, 32(2): 148-152.

[Abstract](727) [PDF 1275KB](661)

Abstract:
OBJECTIVE To study the effects of Bushen Antai Recipe (BAR) on the secretion function and HLA-G expression of human extravillous cytotrophoblasts HTR-8. METHODS SD female rats were treated with BAR or normal saline to prepare for BAR-containing serum or control serum. After centrifugation of the serum， HTR-8 were randomly divided into 4 groups： control group， low-dose BAR (BAR-L) group， medium dose BAR (BAR-M) group and high dose BAR (BAR-H) group. The BAR-L， BAR-M and BAR-H group were treated with 5%， 10% or 20% BAR-containing serum， respectively. The supematants were harvested at 24 h of the culture， and then the levels of hormone， including E₂ (estradiol)， progesterone (P) and human chorionic gonadotropin (HCG)， and regulatory factors of Helper T cells (Th)， including interleukin -4 (IL-4) and interferon gamma (IFN-γ)， were measured by ELISA in culture supernatant. The mRNA of HLA-G was analyzed using quantitative RT-PCR. RESULTS The BAR-L group could promote the secretion of E₂ and P with no statistically significant difference， the BAR-M group and BAR-H group could effectively improve the secretion of E₂ and P， and the BAR-H group is higher than the BAR-M group（P<0.05）.The three groups could improve the HCG secretion effectively（P<0.05），while there were no significant difference among them（P>0.05）. The BAR-L group could promote the secretion of IL-4 with no statistically significant difference（P>0.05）.The BAR-M group and BAR-H group could effectively improve the IL-4 secretion， while there were no significant difference between them（P>0.05）. And the three groups could down-regulate the IFN-γ secretion effectively（P<0.05）. The BAR-M group and high dose group could improve the HLA-G expression effectively（P<0.05)， and the BAR-H group is higher than the BAR-M group（P<0.05）. CONCLUSION The BAR could elevate the secretion in human extravillous cytotrophoblasts and result in the bias response for Th2 type cytokine at the maternal-fetal interface， which might be partially achieved through up-regulating HLA-G expression.

Effects of Component Formula of Salviae Miltiorrhizae Radix Et Rhizome and Ginseng Panax Et Rhizome on Cell Migration， Invasionand and Phosphorylation of ERK1/2 in Lung Cancer A549 Cells

BI Lei, YAN Xiao-jing, YANG Ye, QIU Shuang, CHEN Wei-ping

2016, 32(2): 153-156.

[Abstract](688) [PDF 1265KB](644)

Abstract:
OBJECTIVE To investigate the effects of component formula of Salviae miltiorrhizae radix et rhizome and Ginseng panax et rhizome on cell migration， invasion and phosphorylation of ERK1/2 in lung cancer A549 cells. METHODS The scratch wound healing assay and high content screening were used to analyze the effects of component formula of Salviae Miltiorrhizae Radix Et Rhizoma and Ginseng Radix Et Rhizome on A549 cell migration. The Real-time cell analysis was adapted to detect the effects of component formula on A549 cell invasion. The phosphorylation of ERK1/2 in lung cancer A549 cells was measured by nanoscale ultramicro protein analysis system. RESULTS Component formula treatment significantly increased the distance of scratch blank area in time-dependent manner(P<0.01)， compared with control group; decreased A549 cells migration area(P<0.01) in a dose-dependent pattern; significantly inhibited A549 cells invasion(P<0.01) in time-dependent manner; and decreased the phosphorylation of ERK1/2(P<0.01) in A549 cells. CONCLUSION The component formula of Salviae Miltiorrhizae Radix Et Rhizome and Ginseng Panax Et Rhizome can inhibit A549 cells migration and invasion， which may be related to the decreased phosphorylation level of ERK1/2.

Neuro-Protective Effect and the Mechanism of Semen Euryales Active Fraction Against H₂O₂-Induced PC12 Cells

FANXiu-he, WUQi-nan, DAIShi-lin, GONGYue-song, YUEWei, LIXu-ran, XUHui-qin, YUJin-gao

2016, 32(2): 157-160.

[Abstract](683) [PDF 1075KB](734)

Abstract:
OBJECTIVE To investigate the effect of n-butanol extract from Euryale ferox Salisb. against H₂O₂-induced oxidative injury of PC12 cells and explore the protective mechanism of this active components against Alzheimer's disease cell model. METHODS The n-butanol extracts of semen euryales were separated by the column chromatography of polyamide. PC12 cells were cultivated in vitro and treated with 200 μmol/L H₂O₂for 8 h to establish the oxidative damage model. Cell viabilities were determined by MTT assay， and the activities of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were analyzed in cell-culture medium by the corresponding kits. RT-qPCR was used to detect the mRNA expression of APP， BACE-1 and GSK-3β . RESULTS Compared with the model group， the 50%， 70%， 95% concentration of alcohol in n-butanol components of semen euryales improved the cell viability of PC12(P<0.05). The 70% alcohol components significantly increased SOD activity and decreased the content of MDA in culture supernatants (P<0.05) and significantly down-regulated the expression of GSK-3β (P<0.01). CONCLUSION The 70% and 95% alcohol components of n-butanol extracts of semen euryales shows significant protective effects on oxidative stress-induced cell injury. The protection mechanism may be related to the increased SOD activity， the reduced the emission of MDA， and the inhibition the key genes associated with the accumulation of Aβ.

Effects of Active Components of Epimedium， Astragalus and Radix Puerariae on the Expression of FPN1 in Cerebral Cortex in Alzheimer's Disease Model Mice

DONGXianhui, GAOWeijuan, ZHAOJiaqing, HEXiaoping, CHAIXiqing

2016, 32(2): 161-165.

[Abstract](823) [PDF 1249KB](695)

Abstract:
OBJECTIVE To investigate the effects of the active components of epimedium， astragalus and radix puerariae on FPN1 expression in the cerebral cortex of APPswe/PS1dE9 double transgenic Alzheimer's disease (AD) model mice. METHODS A total of 60 specific pathogen-free male APPswe/PS1dE9 double transgenic mice aged 6 months were equally and randomly divided into the model group， epimedium group， astragalus group， radix puerariae group， compound group and deferoxamine(DFO) group. Additional 10 of 6 months old C57BL/6J mice were chosen as normal control group. After the medication， the mouse brain tissue of every group were collected， and immunohistochemistry， Real time PCR and Western blot were used to detect the effects of active components of epimedium， astragalus and radix puerariae on FPN1 expression in the cerebral cortex of APPswe/PS1dE9 double transgenic mouse model of AD. RESULTS No FPN1 positional cells was observed in negative control group.The mRNA and protein expression of FPN1 decreased in the cerebral cortex of the model group（P<0.05）， compared with the control group. The mRNA and protein expression of FPN1 increased in the compound group and DFO group， compared to the model group. Compared to DFO group， no significant difference was observed in compounde group in the mRNA and protein expression of FPN1 in cerebral cortex; FPN1 mRNA expression decreased in the epimedium group， astragalus group and radix puerariae group. CONCLUSION Active components of epimedium， astragalus and radix puerariae can upregulate FPN1 expression and inhibit the ironoverload in the cerebral cortex of mice with Alzheimer's disease， which will mitigate the iron overload-induced impairment of the central nervous system.

Effect of Shengjiang San on NF-κB Activity in LPS-activated Rat Alveolar Marcrophage Cell Line

QI Ming-ming, MA Jian, ZHAO Feng-ming

2016, 32(2): 166-169.

[Abstract](668) [PDF 1362KB](643)

Abstract:
OBJECTIVE To investigate the effect of Shengjiang San on expression of TLR4 and NF-κB in LPS-induced rat pulmonary alveolar macrophage cell. METHODS NF-κB signal pathway in NR8383 cells was stimulated with LPS. The expression level of TLR4 was measured by QRT-PCR and Western Blotting separately. NF-κB activity was detected by Dual Luciferase Reporter Gene Assay kit. The protein expression level of p-p65 was detected by Western Blotting. The mRNA level of TNF-α，A20，IL-6， IL-1β was measured by QRT-PCR， and the production of TNF-α， IL-6， IL-1β in the supernatant of NR8383 was determined with ELISA. Shengjiang San treated NR8383 cells， which were induced NF-κB signal pathway by LPS. NF-κB activity was detected by Dual Luciferase Reporter Gene Assay kit， qRT-PCR， Western Blotting and ELISA. RESULTS LPS induced TLR overpression in both mRNA and protein levels. Moreover， NF-κB signaling pathway and downstream target cytokines were increased with dose-dependent. 10 μg/mL Shengjiang San treatment abrogated the induction of LPS to TLR4 and NF-κB signaling pathway. CONCLUSION Shengjiang San significantly reduced LPS-activated NF-κB signaling pathway in NR8383.

Effect of Peiminine on MEK1/2， ERK1/2 and the Phosphorylatioin in Lung Tissue of Pulmonary Fibrosis in Rats

GUOHai, JIFuzhi, ZHAOXiaofeng, GONGJiening, QUJinglian

2016, 32(2): 170-175.

[Abstract](719) [PDF 1406KB](714)

Abstract:
OBJECTIVE To investigate the effect of peiminine on MEK1/2， ERK1/2 and the phosphorylatioin of bleomycin-induced pulmonary fibrosis in rats. METHODS Bleomycin of 5 mg/kg was instilled into the rats with a microliter injector to induce the acute lung injury. Shamoperated group and control group were given distilled water of 1 mL/100 g. Dexamethasone group was given equal volume of dexamethasone distilled water solution. Peiminine A and B group were given equal volume of peiminine distilled water solution of 5 mg/kg and 2.5 mg/kg， respectively. After consecutive daily gavage for 28 d， the rats were anesthetized and killed by carotid exsanguination. Lungs were fixed and embedded， and 4 μm sections were prepared. MEK1/2 and ERK1/2 in the lung tissues were detected by immunohistochemistry. The p-MEK1/2 and p-ERK1/2 were detected by Western blot. RESULTS Peiminine significantly reduced the levels of ERK1/2 and MEK1/2 in lung tissues of rats with bleomycin-induced pulmonary fibrosis(P<0.01)， and the effects was similar to dexamethasone (P>0.05). No significant difference was observed between peimine A group and B group(P>0.05). Compared to the model group and dexamethasone group， peiminine significantly reduced the level of p-MEK1/2 and p-ERK1/2 in lung tissue of bleomycin-induced pulmonary fibrosis rats(P<0.01). Compared to peimine A group， the B group displayed more significant efficacy(P<0.01). CONCLUSION Peiminine attenuates the level of MEK1， ERK1 / 2 and the phosphorylation in lung tissues of bleomycin-induced pulmonary fibrosis rats， which may be one of the mechanism of antifibrosis.

Protective Effects of Lemai Granules on Myocardial Ischemia and Its Mechanisms

CAIXiao-jun, SHAONan-qi, FUJin-fang, GAOQing, MAJi-ping

2016, 32(2): 176-180.

[Abstract](667) [PDF 1046KB](632)

Abstract:
OBJECTIVE To observe the effect of Lemai granules on model rats with myocardial ischemia and explore its mechanism. METHODS 48 male SD rats were randomly divided into six groups: normal group， model group， positive control group （Wenxin granules group）and high， middle and low dose group (0.84， 0.42， 0.21 g/kg) of Lemai granules. Myocardial ischemia rats model was established by intraperitoneal injection of isoproterenol (ISO)， and then different doses of Lemai granules were administered intragastrically to the myocardial ischemia rats for 7 d. The changes of the ST-segment of Ⅱlead electrocardiogram (ECG) was observed. The activities of CK， LDH and SOD， the content of MDA， NO， ET， IL-1β，TNF-α and CRP in serum were measured， and changes of blood rheology were tested. RESULTS ST-segment and the activities of CK and LDH of Lemai granules groups(0.84 g/kg， 0.42 g/kg， 0.21 g/kg) were lower obviously than that of model group (P<0.01). Compared with the model group， the activities of SOD and NO in Lemai granule groups of the high and middle doses (0.84 g/kg and 0.42 g/kg) increased in various degrees， while the levels of MDA， ET， IL-1β， TNF-α and CRP were decreased (P<0.05~0.01). The blood rheology were improved significantly. CONCLUSION Lemai granules shows promising protective effects on myocardial ischemia and its mechanism may be related to antioxidant action， regulation of endothelial cells， anti-inflammatory effects and blood rheology improvement.

Identification of Alisma orientale (Sam.) Juzep. and its adulterants using ITS2 sequence

GENGChao, GUWei, WUQi-nan, CHAOJian-guo, SUNHong-mei, JIANGLing, HANYun

2016, 32(2): 181-185.

[Abstract](643) [PDF 1568KB](817)

Abstract:
OBJECTIVE The aim of this study was to identify Alisma orientale (Sam.) Juzep. and its adulterants using the ITS2 barcode. METHODS The total DNAs were extracted from twenty-eight samples of A. orientale as well as adulterants. The ITS2 sequences of these samples were amplified and sequenced. The acquired sequences were submitted to the GenBank and the other ITS2 sequences of 17 samples from ten species were downloaded from the GenBank. Total 45 ITS2 sequences were aligned and the genetic distances among them were analyzed with MEGA 5.1. The sequences analyses were performed using the BLAST1 and the nearest distance methods， and were presented intuitively by constructing Neighbor-joining (NJ) tree. RESULTS The lengths of all ITS2 sequences of A. orientale and A. plantago-aquatica were 311 bp. Only one mutation existed among them. There was significant divergence between the interspecific and intraspecific genetic distances of the ITS2 sequences. The NJ tree showed that A. orientale is obviously different from its adulterants， which showed high monophyly. CONCLUSION As a DNA barcode， ITS2 sequences can stably and accurately distinguish A. orientale from its adulterants and also provide a new clue to identify the medical germplasm resources and ensure the clinical safety in utilization of Chinese materia medica.

Study on Decoction Process and Antifatigue Effect of Polygonatum Lilium Meretrix Decoction

CHENGYing, WANGXin-zhi, LIURui, WUHao, CHENGJian-ming, QIUYun-ying, YANGXiao-lin, CHENLi-ye, FENGZi-fang

2016, 32(2): 186-190.

[Abstract](664) [PDF 1035KB](613)

Abstract:
OBJECTIVE To determine the best water-coction extract technology of Polygonatum Lilium Meretrix Decoction (PLMD) and investigate the anti-fatigue effect of PLMD in mice. METHODS The preperation condition of PLMD was optimized by orthogonal design with extractive yield， amount of polysaccharides and taurine as index. At the same time， mice were randomly divided into six groups: normal group， model group， positive group and administrated groups. In administrated groups， mice were given different doses of PLMD. Swimming model was established after orally administrated daily for 15 days， loaded-swimming time and serum indicators were conducted to explore PLMD's antifatigue effect. RESULTS The best decoction technique was adding 10-fold of water， decocting three times 60minutes each time，in which the polysaccharides and taurine content was (79.48±7.82)， (1.95±0.02) mg/g crude drug， respectively， extractive yield was (26.82±0.53)%. The medium and high doses group of PLMD could obviously prolong the loaded-swimming time， significantly decreased LA， MDA， LDH， CK levels， and the enzyme activity of SOD was ehhanced in dose groups than in model group. CONCLUSION The PLMD’s best decoction process was identified and its antifatigue effect was also confirmed.This result provided the basis for the further development of PLMD.

Comparison of Three Kinds of Flavonoids and Total Flavonoids Contents in Different Organs of Scutellaria Baicalensis Geogi and Its Callus

ZHOU Ping, XIE Chen-qiong, CHEN Jian-wei, LI Xiang

2016, 32(2): 191-194.

[Abstract](744) [PDF 1066KB](712)

Abstract:
OBJECTIVE To compare the contents of three kinds of flavonoids (scutellarin， baicalin and wogonoside) and total flavonoids in different organs of Scutellaria baicalensis Georgi (root， stem， leave anf flower)， medicinal herbs of Scutellaria(tender Scutellaria， old Scutellaria and green Scutellaria) and its callus. METHODS SYMMETRY C₁₈（4.6 mm×250 mm，5 μm） column was used with the solvent system of acetonitrile (A)-0.22% acetic acid water (B) and the UV detection wavelength of 335 nm， at the temperature of 35 ℃， to detect the contents of three kinds of flavonoids. The contents of total flavonoids were determined with the control sample of rutin， the indicators of aluminum nitrate and the UV detection wavelength of 504 nm. RESULTS The linear ranges of scutellarin， baicalin and wogonoside were 6.0~192.0， 30.0~960.0， 12.0~384.0 μg/mL（R²≤0.998 9）， respectively. The average recoveries were 97.8%~103.1%， RSD≤4.0%. The linear ranges of total flavonoids was 40.0~240.0 mg/mL（R²≥0.999 8）， and the average recoveries was 98.8%(RSD=1.0%) . CONCLUSION The content of scutellarin in aboveground Scutellaria baicalensis Georgi (flower， stems and leaves) was higher than underground part (root)， and the content in flowers is highest， reaching the point of 0.53%. Baicalin and wogonoside content in underground part of are higher than aboveground， and the content in callus baicalin reaches 0.60%. In callus， the content of total flavonoid is highest (30.42%). Those results may provide a basis for scutellarin extraction from Scutellaria flower and the production of total flavonoids from Scutellaria callus.

Professor Zhou Zhongying's Clinical Experience and Theory in Treating Intracranial Tumors from the Perspective of Treatment based on Pathogenesis

LIANGBing, SUNJingyun, YEFang

2016, 32(2): 195-197.

[Abstract](633) [PDF 993KB](722)

Abstract:
This paper introduces Professor Zhou Zhongying's clinical experience and theory in treating intracranial tumors. The pathogenesis of intracranial tumors is deficiency of liver and kidney， wind-phlegm obstruction and stasis， obstruction of clear yang. When dealing with the disease， Professor Zhou cares about both the disease and sign， the differentiation of pathogenesis and treats the disease on the basis of pathogenesis differentiation. As for the treatment， Professor Zhou emphasize the priority of solving asthenia in superficiality rather than the asthenia in origin， expelling the evil rather than strengthening vital qi.

Clinical Experience of Professor Huang Huang in Treating Lung Cancer by Using ShuyuWan

MIAO Ting-ting

2016, 32(2): 198-200.

[Abstract](739) [PDF 996KB](1263)

Abstract:
This paper introduces Professor HuangHuang's clinical experience in treating lung cancer by using ShuyuWan. Professor HuangHuang is the famous TCM physician of Jiangsu. It is considered that the pathogenesis of lung cancer is deficiency of Qi and blood with the wind pathogen. In this disease， the lung， spleen and kidney are usually affected. As for the treatment of lung cancer， its basic therapeutic methods are regulating Qi and blood， strengthening vital Qi and eliminating pathogens. Professor HuangHuang often uses modified ShuyuWan to treat lung cancer in clinical practice. Drug dosage often varies based on the patients' diseases and the condition of the specific circumstances.

2016 Vol. 32, No. 2

ArchiveMore+

Author field More+

Edit fieldMore+