2016 Vol. 32, No. 1

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Exploration of Professor ZHOU Zhongying's Experience in Syndrome Differentiation and Treatment of Malignant Tumor in Digestive Tract
ZHAO Zhi-qiang
2016, 32(1): 1-3.
[Abstract](802) [PDF 1012KB](717)
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Common types of malignant tumors in digestive tract as esophageal cancer， gastric cancer， colon cancer and rectal cancer can be observed in professor ZHOU Zhongying's clinical practice， with each case presenting different manifestations. The main processes in pathogenesis include accumulation of cancer toxin， dysfunction of stomach in digesting food and transporting food downward， conduction disorder of intestine in receiving， resolving and separating the useful from the waste. And the formula prescribed are aimed to fight against cancer， purify toxin， remove phlegm， eliminating stasis， regulate spleen and stomach， replenish qi and nourish yin， with corresponding tumor characteristics being taken into consideration.
Study of Gout from the Perspective of Turbidity and Stasis Retention and the Disharmony of Spleen and Kidney
JIANGTian, GUDong-mei, JIANGHan-rong, ZHUWan-hua
2016, 32(1): 4-5.
[Abstract](692) [PDF 999KB](729)
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Professor ZHU Liangchun pointed out that gout is mainly caused by turbidity and stasis retention and the disharmony of spleen and kidney. In the framework of turbidity and stasis theory， this paper focused on the relation between gouty inflammation and turbidity and stasis retention， immune response and the disharmony of spleen and kidney.
The Study of Gene Network of Yin Deficiency and Yang Excess Syndrome of Essential Hypertension
YUANYe, SHENChun-ti, CHENBing-wei
2016, 32(1): 6-9.
[Abstract](744) [PDF 1435KB](642)
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OBJECTIVE To study the gene network of yin deficiency and yang excess syndrome of essential hypertension to support the syndrome differentiation. METHODS Genes related to yin deficiency and yang excess syndrome of essential hypertension were collected from the literatures and CYTOSCAPE was used for the network construction and MCODE for modularized analysis. After that， a functional enrichment analysis was performed to find the biological processes and pathways of the most important modules. RESULTS The modules containing most genes had 17 genes which were related to vascular remodeling， vascular smooth muscle cell proliferation regulating， apoptosis regulating， MAPK signal pathway， Toll-like receptors signal pathway and NOD-like receptors signal pathway. VEGFA， ACE and MAPK9 had the highest genetic link frequency. CONCLUSION VEGFA， ACE and MAPK9 have the strongest connection in the network. Vascular remodeling has the strongest connection with MAPK signal pathway.
Effect of Electro-acupuncture Deep Needling on Spontaneous Bowel Movements and Life Quality of Female Patients with Severe Functional Constipation
CHENLu, SUNJian-hua, WUXiao-liang, HANGui-hua
2016, 32(1): 10-13.
[Abstract](679) [PDF 1123KB](601)
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OBJECTIVE To observe the effect of electro-acupuncture on spontaneous bowel movements and life quality of female patients with severe functional constipation. METHODS A total of 53 female patients with severe functional constipation were randomly divided into two groups. One group was treated with deep needling and electro-acupuncture (EA)， while the other group with shallow needling and sham electro-acupuncture (sham-EA). The numbers of spontaneous bowel movements (SBMs) and patient assessment of constipation quality of life questionnaire (PAC-QOL) scores of the two groups were recorded after 4 weeks and 8 weeks of treatment and the differences of the two groups were compared. RESULTS The spontaneous bowel movements of the two groups were both improved. The improvement of the EA group was more statistically significant (P<0.01). After receiving treatment for 4 weeks and 8 weeks， the improvement of the PAC-QOL scores of the EA group was more significant(P<0.01). CONCLUSION Electro-acupuncture treatment could improve the spontaneous bowel movements and life quality of female patients with severe functional constipation and the deep needling electro-acupuncture is more effective.
Guipi Pills in Treating Anemia of Patients Receiving Hemodialysis and the Influence on the Inflammatory Factors
SUNJie, MAJun-jie, ZHANGYi-lai, WEIShan-zhai
2016, 32(1): 14-16.
[Abstract](735) [PDF 1031KB](624)
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OBJECTIVE To study on the influence of Guipi pills on anemia and the inflammatory factors of patients receiving hemodialysis. METHODS 60 patients diagnosed with CKD(stage 5)， CKD anemia and spleen deficiency and blood deficiency and have maintenance hemodialysis(MHD) were randomly divided into two groups with 30 cases in each group. The control group was treated with erythropoietin(EPO) and the treatment group， besides receiving EPO， took Guipi pills as well. The treatment lasted for 60 days. The hs-CRP， IL-6 and Hb were recorded before and after the treatment. RESULTS After the treatment， hs-CRP， IL-6 and Hb of both groups had significant decrease(P<0.05~0.01) and the treatment group was better than the control group(P<0.05~0.01). After the treatment， Hb of both groups increased(P<0.05~0.01) and still the treatment group was better than the control group(P<0.05). CONCLUSION Guipi pills are effective in treating anemia and inflammation of patients receiving hemodialysis.
Effects of NBUVB Combined with Tuibai Granule on CD4+/CD8+ Tlymphocyte Ratio and CD4+CD25+ Treg in the Peripheral Blood of Vitiligo Patients
ZHAO Jing, SHENG Guo-rong, YAN Xia, XIE Yong
2016, 32(1): 17-20.
[Abstract](770) [PDF 1052KB](720)
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OBJECTIVE To detect the expression levels of CD4+， CD8+ Tlymphocytes and CD4+ CD25+ Treg in the peripheral blood of patients with vitiligo after the treatment of NBUVB combined with Tuibai Granule， and then explore the relationship between vitiligo pathogenesis and the levels of CD4+， CD8+ Tlymphocytes as well as CD4+ CD25+ Treg. METHODS The expression levels of CD4+， CD8+ Tlymphocytes and CD4+ CD25+ Treg were detected by FlowCytometry among 120 cases of vitiligo patients and 30 cases of healthy control group. Then the 120 cases were further divided into three groups， with 40 cases in each group. Patients in the treatment group were treated with NBUVB combined with Tuibai Granule; Patients in the control groupⅠwere only given NBUVB， while those in the control groupⅡonly took Tuibai Granule. After 3 months treatment， the changes of the CD4+/CD8+ ratio and the expression levels of CD4+ CD25+ Treg were once again tested and the comparison was made among the four groups. RESULTS The CD4+ Tlymphocytes level in the peripheral blood decreased significantly， while the CD8+ Tlymphocytes level increased evidently. There were significant statistical differences when compared with the healthy control group(P<0.01); The CD4+/CD8+ ratio of the progressive stage was much lower than that of the stable stage(P<0.01); the CD4+/CD8+ ratio of the stable stage had no statistical difference when compared to that of the healthy control group(P>0.05). The frequency of CD4+， CD25+ Treg of vitiligo patients was significantly lower than that of healthy control group(P<0.01)， and the frequency of CD4+， CD25+ Treg of the progressive stage was also lower than that of the stable stage(P<0.01). After treatment with NBUVB combined with Tuibai Granule， the CD4+/CD8+ ratio and the frequency of CD4+， CD25+ Treg were significantly up-regulated compared with either the control groupⅠor the control groupⅡ(P<0.01，P<0.05). CONCLUSION These findings suggest that the abnormal level of CD4+/CD8+ ratio and the frequency of CD4+， CD25+ Treg might be involved in the vitiligo pathogenesis. NBUVB combined with Tuibai Granule can prevent vitiligo by regulating the CD4+/CD8+ ratio and the frequency of CD4+， CD25+ Treg.
Clinical Research on Micro-invasive Acupoint Thread-embedding Therapy in Treating Refractory Prosopoplegia
DING Min, FENG Hua, JIN Chang-xu, XU Lei, ZHANG Jie, LIN Tian-yun
2016, 32(1): 21-24.
[Abstract](715) [PDF 1049KB](696)
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OBJECTIVE To compare the clinical effects of acupuncture therapy， catgut therapy and PDS therapy in treating refractory prosopoplegia. To observe the effects and side effects of the two kinds of threads. METHODS 228 patients were randomly divided into three groups: acupuncture group， PDS group and the catgut group， with 76 patients in each group. Zanzu(B2)， Yuyao(Extra)， temple， Dicang(ST4)， Jiache(S6)， Qianzheng， Xiaguan， Fengchi(G20)， Hegu(L14) on both sides， Zusanli(ST36) were chosen in the three groups. Sunnybrook facial nerve evaluation system and ENoG were used before and after the treatment to asses the effect. And the side effects of the two groups with different threads were observed right after the treatment， and after ten and thirty days. RESULTS After the treatment， scores of Sunnybrook facial nerve evaluation system of three group all improved(P<0.05). The lateral frontal muscle， orbicular muscle of mouth showed improvement in ENoG after the treatment(P<0.05).The difference between the two group in side effects like tenderness， induration and red and swollen had no statistical significance(P>0.05). Ten days after the treatment， more patients in the catgut group had tenderness， induration and red swollen than the PDS group and the difference had statistical significance(P<0.01). Thirty days after the treatment， there were more patients in the catgut group had tenderness than the PDS group and the difference had statistical significance(P<0.05). Because there were less induration and red and swollen， the results showed that there were more induration and red and swollen in the catgut group than the PDS group and the difference was of stastical significance(P<0.01). CONCLUSION Acupuncture， catgut and PDS thread-embedding therapy are all effective in treating refractory prosopoplegia. And the two threat-embedding therapies can effectively treat the disease and shorten the treating period. PDS thread is a good embedding material which can lower the risks of side effects.
Effects of Gancao Xiexin Decoction on Serum Levels of IL-17 and IL-23 in Patients with Ulcerative Colitis
WANG Guo-qing, WEI Wen-hong, YANG Jie
2016, 32(1): 25-28.
[Abstract](915) [PDF 1044KB](668)
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OBJECTIVE To explore the treatment mechanism of Gancao Xiexin Decoction on patients with ulcerative colitis(UC) by observing the serum levels of interleukin-17(IL17)， interleukin-23(IL23) before and after the treatment. METHODS 60 patients with UC were randomly divided into control group and treatment group， with 30 patients in each group. Patients in the control group were given oral sulfasalazine， while those in the treatment group were given Gancao Xiexin Decoction combined with sulfasalazine. After 8 weeks treatment， the clinical efficacy evaluation was done. To be specific， the disease activity score was calculated according to Mayo Score， and the quality of life was measured by IBD Questionnaire(IBDQ) before and after the treatment. Besides， peripheral venous blood samples of patients in both groups were collected and the serum levels of IL17， IL23 were measured with enzyme-linked immunosorbent assay(ELISA). RESULTS The total effective rate of the treatment group was 93.3%， which was evidently superior to that of 80% in the control group(P<0.05); The Mayo score of both groups went down after the treatment(P<0.01)， and the score of the treatment group was much lower than that in the control group(P<0.01). The symptom scores such as intestinal tract symptom， constitutional symptom， emotional capability， social competence as well as the total scores of the IBDQ in the treatment group were higher than those in the control group(P<0.05~0.01). The serum levels of IL17、IL23 in both group declined after the treatment， while the decrease in the treatment group was more obvious compared with the control group(P<0.01). CONCLUSION Gancao Xiexin Decoction combined with sulfasalazine shows significant efficacy in improving the clinical symptoms and life quality of patients with UC. One of the action mechanisms of Gancao Xiexin Decoction in treating UC might be its effective role in decreasing the serum levels of IL17， IL23 and participating in immune regulation.
Clinical Research on Qingdouling Mixture in Treating Chronic Rhinitis-sinusitis in Children
LILiang-bo, GONGCheng, LIAOYong, YUANWei, WANGZhen-duan, JIANGJin-lin
2016, 32(1): 29-31.
[Abstract](829) [PDF 1028KB](615)
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OBJECTIVE To observe the clinical efficacy of Qingdouling Mixture in treating chronic rhinitis-nasosinusitis in children. METHODS One hundred children cases with chronic rhinitis-sinusitis were randomly divided into treatment group and control group， with 50 in each. And the control group were treated with routine western treatment (antibotics for oral administration， nasal nitric oxide and mucolytics)， and control group were treated with both the above medications and Qingdouling Mixture. We made 6-month follow-ups constantly and observed clinical efficacy of 2 groups. RESULTS Compared to control group， the treatment group got more significant improvement in symptoms， body signs and state of illness. There is statistical significance between them(P<0.05). CONCLUSION Qingdouling Mixture is effective in treating chronic rhinitis-nasosinusitis in children with little recurrences and side effects.
Clinical Research on Acupuncture Combined with Medicine on Luteinized Unruptured Folicle Syndrome
WANG Li-hong, XU Wen-ting
2016, 32(1): 32-34.
[Abstract](667) [PDF 1020KB](640)
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OBJECTIVE To observe the clinical efficacy of treating luteinized unruptured folicle syndrome(LUFS) with Nourishing Kidney to Promote Ovulation Decoction， human chorionic gonadotropin(HCG) as well as acupuncture. METHODS 87 patients with luteinized unruptured folicle syndrome were randomly divided into two groups. 45 cases in the treatment group were given Nourishing Kidney to Promote Ovulation Decoction， HCG combined with acupuncture， while 42 cases in the control group were only given Nourishing Kidney to Promote Ovulation Decoction and HCG. Then the pregnancy rate， ovulation rate and the levels of luteinizing hormone(LH)， Estradiol(E2)， progesterone(P) were observed so as to make a comparasion of the clinical effects between these two groups. RESULTS The treatment group was more superior to the control group in improving the levels of LH， E2 and P， pregnancy rate and ovulation rate(P<0.05). CONCLUSION Nourishing Kidney to Promote Ovulation Decoction， HCG combined with acupuncture can treat patients with LUFS effectively. The mechanism of the therapy may be related to its role of improving the endocrine situation.
Effects of Electro-acupuncture on Serum Leptin Content and Expression of Kctd15 and Sh2b1 mRNA in Hypothalamus in Stat5-knockout Induced Obese Mice
HONG Hao, LU Sheng-feng, FU Shu-ping, OU Chen, ZHU Bing-mei
2016, 32(1): 35-37.
[Abstract](665) [PDF 1090KB](655)
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OBJECTIVE To observe the effect of electro-acupuncture on central conditional knockout Stat5 Nestin-Cre obese mice (Stat5NKO) and detect how the obesity related genes， Kctd15 and Sh2b1， are involved. METHODS 20 obese Stat5NKO mice were randomly divided into two groups: obese model group (n=10) and electro-acupuncture （EA） group (n=10); 10 Stat5fl/fl mice were randomly selected and fed with ordinary forage as normal control group. EA group was treated with electro-acupuncture at Housanli and Neiting points for 30 min， with bilateral alternates， by Han' electro-acupuncture apparatus at 2/15 Hz. 6 d was a period of treatment， with a total of 4 courses of treatment. Serum Leptin content was measured by ELISA， and the levels of Sh2b1 and Kctd15 mRNA in hypothalamus were detected by qPCR. RESULTS Compared with Stat5fl/fl mice， Stat5NKO mice gained body weight significantly (P<0.01)， accompanied with increased serum Leptin level (P<0.05)， decreased hypothalamic Leptin level (P<0.05)， as well as Kctd15 and Sh2b1 mRNA expressions (P<0.05~0.01); Compared with Stat5NKO mice， EA group decreased body weight (P<0.01) and serum Leptin mRNA level (P<0.01). The hypothalamic Leptin level was elevated (P<0.01)， and the expressions of Kctd15 and Sh2b1 mRNA were returned to normal level (P<0.01). CONCLUSION The weight-loss effect of electro-acupuncture on Stat5NKO obese mice may be related to the regulation of the expression of Leptin， Kctd15 and Sh2b1.
Effects of Bushen Cupailuan Decotion on Granular Cell Aromatase in Polycystic Ovary Syndrome Model Rats
GUOYin-hua, TANYong, ZOUYi-jie
2016, 32(1): 38-40.
[Abstract](696) [PDF 1171KB](742)
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OBJECTIVE To explore the effect and working mechanism of Bushen Cupailuan Decotion on ovarian granulosa cells aromatase of polycystic ovary syndrome (PCOS) model rats. METHODS Bushen Cupailuan Decotion was taken by PCOS model rats which were induced by dehydroepiandrosterone (DHEA). RT-PCR and immunohistochemical methods were used to detect the mRNA and protein expression of granulosa cell aromatase in PCOS model rats. RESULTS Bushen Cupailuan Decotion significantly improved the mRNA and protein expression of granulosa cell aromatase in PCOS model rats (P<0.05). CONCLUSION Bushen Cupailuan Decotion can improve the expression of ovarian granulosa cell aromatase in PCOS model rats， which could be one of the mechanism of Bushen Cupailuan Decotion on promoting follicular development and releasing.
Effects of Yanghe Pingchuan Granules on the Expression of AQP1 and AQP5 in Rats with Bronchial Asthma
DING Wei-wei, YANG Cheng, TONG Jia-bing, XU Zeng-mei, LU Mei, TONG Xiang-li, LI Ze-geng
2016, 32(1): 41-44.
[Abstract](707) [PDF 1856KB](998)
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OBJECTIVE To investigate the effects of Yanghe Pingchuan Granules on the expression of AQP1 and AQP5 and explore the mechanism of Yanghe Pingchuan Granules in treating asthma from regulating water metabolism and airway mucus secretion. METHODS 90 SD rats were randomly divided into six groups which were the normal group (N)， asthma model group(M)， low， medium and high-dose of Yanghe Pingchuan groups(YHPC-L， YHPC-M and YHPC-H) and Dexamethasone group(DXM). The M， YHPC-L， YHPC-M， YHPC-H and DXM groups were sensitized with OVA by intraperitoneal injection and inhalation， then Hydrocortisone was used to replicate kidney-yang deficiency model， while the N group was treated with saline as control. HE staining was used to observe the pathological changes in lung tissues. Real-time PCR and Western blot was applied to detect the mRNA and protein expression of AQP1 and AQP5 in lung tissue， respectively. Immunohistochemical was applied to examine the AQP1 and AQP5 distribution and expression in lung tissue. RESULTS The mRNA and protein expression of AQP1 and AQP5 in M group was significantly lower than in N group (P<0.01). Compared with M group， the expression of AQP1 and AQP5 increased highly after treated with low， medium and high-dose of Yanghe Pingchuan Granules (P<0.05~0.01 ) with dose-dependent effect. Meanwhile， the effect of low-dose Yanghe Pingchuan Granules was lower than DXM (P<0.05)， but the medium and high-dose of Yanghe Pingchuan Granules had a considerable effect (P>0.05). CONCLUSION Yanghe Pingchuan Granules could maintain water homeostasis and regulate airway mucus secretion by up-regulating the expression of AQP1 and AQP5.
Effects of Acupuncture Oninsulin Resistance and Morphologic Changes of Islet β-cell in Rat with Type 2 Diabetes Mellitus
WANGXu, ZHANGZhi-long, YANGYuan-qing, CHENHong, LUXuan, ZHANGJian-bin, SUHong-yan
2016, 32(1): 45-49.
[Abstract](644) [PDF 1320KB](612)
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OBJECTIVE To study the effects of Regulating spleen-stomach on acupuncture on insulin resistance (IR) and morphologic changes of islet β-cell in rat with type 2 diabetes mellitus (T2DM). METHODS 10 rats were randomly selected into normal group from thirty-six male Wistar clean-grade rats. The rest rats were made The T2DM rat model，which established with streptozotocin(25 mg/kg) and high fat diet. Twenty rats were made T2DM rat model successfully，which divided into acupuncture group (10 rats)and model group(10 rats)，according to Stratified randomization. Normal group were not given any treatment. Acupuncture group were treated with acupuncture that were dressed in clothes which reduce restless.and the treatment was given for continuous 4 weeks.But model group were not given any treatment. Several outcome indicators were measured such as body mass， fasting blood-glucose(FBG)， 2 hour postprandial blood glucose(P2BG)， fasting insulin(FINS)， insulin sensitivity index(ISI).After the last intervention，the rats were killed and pancreas were made HE staining. RESULTS One weeks after the intervention， body mass of acupuncture group increased significantly compared with normal group（P<0.05），which had no significantly difference with model group（P>0.05）. Body mass of model group were significantly reduced compared with normal group and acupuncture group at the four weeks after the intervention（P<0.05）. The FBG and P2BG of acupuncture group were lower than model group at the every weeks after the intervention（P<0.01）.FINS of model group and acupuncture group were higher than normal group front the intervention（P<0.01），which increased significantly compared with other groups in the ISI（P<0.01）. FINS of model group were higher than acupuncture group and normal group after the intervention（P<0.01），what is more，ISI of acupuncture group increased significantly compared with model group（P<0.05）. Acupuncture group has protective effects of morphology of islet β-cell. CONCLUSION Regulating spleen-stomach on acupuncture could reduces the level of FBG， P2BG and FINS and improves ISI and relieve IR， which maintains rats of body mass promoting restoration of islet and β-cell.
Effects of Acupuncture Combined with Rehabilitation Training on Neurological Deficits and Endogeneous Nerve Stem Cells in Adult Rats with Brain Ischemic Injury
HU Ke, REN Ying-guo, XU Ming-chao, ZHANG Bao-chao, JIA Dong-pei
2016, 32(1): 50-53.
[Abstract](669) [PDF 1043KB](598)
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OBJECTIVE To study of the effect of acupuncture combined rehabilitation training on neurological deficits and endogeneous nerve stem cells in adult rats with brain ischemic injury. METHODS Rats model of middle cerebral on occlusion were established and were divided into model group，rehabilitation training group and acupuncture combined rehabilitation training group. Neurological scores， behavinnd function and the proliferation of rats nerve stem cells were detected after 24 h， 7 d， 14 d， 21 d. RESULTS There were no significant differences in three groups in Neurological scores， behavinnd function after 24 h. Significant differences were found in Neurological scores， behavinnd function after 7 d， 14 d， 21 d (P<0.05). Brdu positive cells proliferation were at the highest peak in 7 d，and there were significant increase in rehabilitation training group and acupuncture combined rehabilitation training group than model group (P<0.05). CONCLUSION The treatment of acupuncture combined rehabilitation training can improve Neurological scores and behavinnd function and can induce proliferation of nerve stem cells.
Experimental Study on the Anti-gastric Ulcer Action of the Extract of Geranium wilfordii Maxim
LIYi, LIULi-ming, LIChao, WANGYan, ZHANGXu, LIYang
2016, 32(1): 54-57.
[Abstract](737) [PDF 1034KB](713)
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OBJECTIVE To study the action of the extract of Geranium wilfordii Maxim on the experimental gastric ulcer and to investigate the potential mechanism. METHODS Pyloric ligation， acetic acid， indomethacin and ethanol induced gastric ulcer model were applied to observe the anti-gastric ulcer action of the extract of Geranium wilfordii. RESULTS The extract of Geranium wilfordii significantly reduced the gastric ulcer index in pylorus-ligated， acetic acid induced， ethanol induced and indomethacin induced gastric ulcers. In pylorus-ligated rats， the extract also reduced gastric juice volume， gastric juice acidity and pepsin content. CONCLUSION The extract of Geranium wilfordii increases healing of gastric ulcer and also prevents development of gastric ulcer in rats.
Pharmacodynamic Effects of Tongbian Decoction on Slow Transit Constipation Model
XUTian-shu, QIANHai-hua, ZHOUQing, MENGJun
2016, 32(1): 58-61.
[Abstract](732) [PDF 1175KB](914)
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OBJECTIVE To observe the effect and mechanism of Tongbian Decoction on slow transit constipation Model. METHODS SD rats were divided into normal control group and model group. Normal control group were given distilled water， while the model group was treated by laxative colon method and divided into natural recovery group， mosapride groups and Tongbian Decoction soup. After the drug intervention of 2， 4 and 6 weeks， dejection amounts in colon， the rate of carbon powder proposed， colonic electrical test and the morphological observation of colon interstitial cells of Cajal (ICC) were measured. RESULTS Compared to natural recovery group，Tongbian Decoction group significantly alleviated the constipation symptoms of functional constipated rats， enhanced the contraction of colon tissue， promoted intestinal peristalsis and improved the damaged intestinal tissue cells (P<0.01). CONCLUSION Tongbian Decoction can promote the colonic transit activities of slow transit constipation model rats.
Preparation of BaohuosideⅠ-loaded Mixed Micelles and Its Anti-tumor Activity
WEIYu-jian, LOUBin, WANGXin-dong
2016, 32(1): 62-66.
[Abstract](685) [PDF 1167KB](615)
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OBJECTIVE Preparation of baohuosideⅠ-loaded mixed micelles and its anti-tumor activity. METHODS BaohuosideⅠ-loaded Solutol HS 15/Poloxamer 188 mixed micelles (BSPM) was prepared by thin-film hydration method and the preparation process of BSPM was optimized by orthogonal design. The formulation of BSPM was evaluated and the in vitro release behavior of BSPM was determined. Cell toxicity and uptake assays were employed to evaluate the anti-tumor activities. The targeting effect of BSPM was studied by fluorescence labeling. RESULTS The BSPM prepared showed spherical and regular shape， with an average particle size of 33.59 nm and its entrapment efficiency more than 80%. The 24 h-accumulated release ratio in vitro of BSPM was up to 82.56%. Cytotoxicity of BSPM on human breast cancer cell line MDA-MB-231 was significantly stronger than that of free baohuosideⅠ and the uptake rate was improved. CONCLUSION The BSPM which shows small particle size and high entrapment efficiency can enhance cell toxicity and uptake. It is an effective and promising formulation for anti-tumor drug.
Study on Preparation Method and Endogastric Bioadhesion Properties of Berberine-carbomer Salt
ZHAO Yan, LIU Tao-shi, CHENG Jian-ming, BI Xiao-lin, CAI Bao-chang
2016, 32(1): 67-71.
[Abstract](747) [PDF 1216KB](728)
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OBJECTIVE To study preparation method and drug releasing in vitro and endogastric bioadhesion properties and killing Hp activity in vitro of berberine-carbomer salt. METHODS Influencing factor such as variety of carbomer， the concentration of carbomer and berberine are studied by single factor experiment using the yield and the drug loading as evaluating indicator. The release rate of berberine from berberine-carbomer salt in vitro are measured by using Chinese Pharmacopeia (CP) 2010 oar method. The endogastric bioadhesion of berberine-carbomer salt are evaluated by determining the adhesive force of berberine-carbomer salt with the rat gastric mucosa in vitro， and the remaining percentage on the rat’s gastric mucosa in vitro or in rat’s stomach in vivo. The killing Hp activity in vitro of berberine-carbomer salt are evaluated by cylinder plate method. RESULTS The optimizing variety is carbomer 974P， and the concentration of carbomer 974P is 0.25%， berberine is 0.3%， with the ratio between carbomer solution and berberine solution being 2∶1. The releasing of berberine-carbomer salt indicates the delayed releasing properties in the dissolution mediator with pH 1.2. The adhesive force of berberine-carbomer salt with the rat gastric mucosa and the remaining percentage on the rat’s gastric mucosa in vitro are larger than berberine. The endogastric stagnation time in rat of berberine-carbomer salt is longer than berberine. CONCLUSION Berberine-carbomer salt possess superordinary properties of delayed releasing and endogastric bioadhesion.
Comparing Metabolites of Liguzinediol in Liver Microsomes among Different Species
DAI Zhen-li, WEN Hong-mei, SHAN Chen-xiao, YOU Xiao-qin, DONG Bang, SUN Xue, LI Wei
2016, 32(1): 72-75.
[Abstract](783) [PDF 1506KB](625)
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OBJECTIVE To compare metabolites of liguzinediol in liver microsomes among different species， such as rats， dogs， monkeys and human. METHODS LC-MS/MS method was established to determine liguzinediol and its metabolites. The liver microsome incubation systems among different species were built to research the metabolites of liguzinediol. RESULTS Phase Ⅰ and phase Ⅱ metabolism of liguzinediol can be occurred in liver. The oxidation and glucuronidase acid binding products were all found in four kinds of liver microsomes. CONCLUSION The metabolites of liguzinediol in liver microsome from rats， dogs and monkeys were consistent with that from human. The results can be used to select laboratory animal for safety evaluation of liguzinediol in preclinical study.
Identification of Main Components in Water Decoction of Xiefei Pingchuan Ling with HPLC
WANGYue-jia, CHAOJian-guo, HANXin-min, GUWei, LIUJu-yan, SUNYa-xin, LIMeng-yang
2016, 32(1): 76-78.
[Abstract](700) [PDF 1071KB](677)
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OBJECTIVE To establish the chromatographic method of Xiefei Pingchuan Ling water decoction， and analyze its main components. METHODS The retention time of sample and standards were compared by gradient elution chromatography method. Acetonitrile-0.2% formic acid aqueous solution (gradient elution) was used as mobile phase at a flow rate of 0.8 mL/min. The detection wave length was 254 nm and column temperature was 30 ℃. RESULTS 18 components were separated from Xiefei Pingchuan Ling extract， and ephedrine， pseudoephedrine hydrochloride， salvianolic acid B， rhein， aloeemodin and chrysophanol were identified. CONCLUSION The established HPLC method shows high stability and good reproducibility， and provides a basis for further study on serum pharmaceutical chemistry and structure-activity relationship.
Analysis of Fructus Corni by Infrared Spectroscopy and Maillard Reaction during Processing
LUYan, ZHANGYu-fei, YANGGuang-ming, PANYang, CAIBao-chang
2016, 32(1): 79-82.
[Abstract](706) [PDF 1077KB](662)
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OBJECTIVE To study the FT-IR characterization of components of Fructus Corni and the relationship between information in IR and Maillard reaction during processing. METHODS Fructus Corni was processed by steaming with wine for 48 h. During processing， samples processed at different time points were collected to carry out infrared spectrum analysis. RESULTS It could be deduced from the spectra that there were alcohols， organic acids， esters and so on in Fructus Corni. When being processed， the amounts of components changed slightly and stabilized gradually with main peaks at 3309 cm-1， 1713 cm-1 and 1028 cm-1. During processing， the content of amine-group compounds showed an upward trend in 0~24 h， while showed an opposite trend in 24~48 h， therefore 24 h could be regarded as the optimal processing time in consideration of IR information and results of our previous study of Maillard reaction of Fructus Corni. CONCLUSION This infrared spectroscopy method is simple and convenient to analyze Fructus Corni samples during processing， and can preliminary recognize the main ingredients in samples. Combined with the study of Maillard reaction of Fructus Corni， it could provide useful references for the optimization of processing of Fructus Corni.
Effects of Different Decocting Time on the Content of Inorganic Element in Jieyu Shunxin Decoction
WANGBin, CAIHao, QINKun-ming, ZHANGMiao, WANGYu, JINJun-jie, CAIBao-chang
2016, 32(1): 83-86.
[Abstract](649) [PDF 1041KB](632)
Abstract:
OBJECTIVE To determine mineral elements content in Jieyu Shunxin Decoction (JYSXD) by ICP-AES，and study the effect of different decocting time on the solution of mineral elements in JYSXD. METHODS Mineral elements (K， Ca， Na， Mg， Al， Zn， Fe， Sn， B， Li， Sr， Cd and As) in JYSXD which was boiled for 30， 60， 90 min， respectively， were detected by ICP-AES after HNO3/H2O2 digestion. RESULTS After JYSXD was boiled for 30 min， each element was not able to completely precipitated. However， after the decoction was boiled for 60 min， all the elements were completely precipitated. K， Ca， Na， Mg， Al， Zn， Fe， Sn， B， Li and Sr were detected， while Cd and As were not found. Among them， K was the highest in the content of major elements and Zn was the highest in trace elements. Each mineral elements in JYSXD showed good linear relationship (r>0.999 0)， RSD of precision， stability and repeatability test all satisfied the requirement of quantitative analysis. The recovery was 95.07%~100.83% (RSD<3%). CONCLUSION In order to reduce the decocting time and maintain the solution of mineral elements in JYSXD as much as possible， the decocting time should be controlled at 60 min. The experimental results provide basis for material basis and pharmacodynamics study of Jieyu Shunxin Decoction.
Professor WANG Shouchuan's Clinical Experience in Treating Pediatric Tonsillitis
HE Li-li
2016, 32(1): 87-89.
[Abstract](686) [PDF 1018KB](863)
Abstract:
According to professor Wang Shouchuan's clinical experience， pediatric tonsillitis can be divided into acute tonsillitis and chronic tonsillitis based on syndrome differentiation， and the treatment principle is to resolve toxin and disperse swelling. The acute tonsillitis can be further divided into the syndrome of toxin congesting lung and throat and syndrome of muscle fester based on the clinical feature， with the former being treated by dispelling wind， clearing away heat， removing toxin to sooth throat while the latter being treated with detoxication， carbuncles elimination， drainage of pus to sooth throat. As for chronic tonsillitis， it can also be further divided into the syndrome of toxin retained in throat and syndrome of qi deficiency and blood stasis. The method of soothing throat， dispersing swelling， removing toxin to eliminate stagnation should be applied to the former type， while the method of tonifying qi， nourishing yin， activating blood circulation to disperse swelling should be used to the latter type.
Professor LUO Songping's Experience in Nourishing the Origin in Advance and Strengthening Chong to Prevent Miscarriage
GAOFei-xia, ZHULing, GAOJie
2016, 32(1): 90-92.
[Abstract](642) [PDF 1018KB](1159)
Abstract:
This paper introduced Professor LUO Songping's experience in treating elderly parturient women with abnormal pregnancy history by following the principal of nourishing the origin in advance. Elderly parturient women with abnormal pregnancy history usually suffer from kidney qi failure， oligomenorrhea， and insufficiency of Chong-Ren. It's difficult for them to be pregnant. If pregnancy occurs， they are likely to have miscarriages. So in clinical practices， methods like tonifying the kidney to strengthen Chong-Ren， nourishing blood to regulate Chong-Ren， dispersing liver and promoting blood circulation to regulate Chong-Ren are often used before the pregnancy. Methods like strengthening the kidney to nourish Yuan and nourishing blood to prevent miscarriage are applied during pregnancy.
The Research Progress in TCM Treatment of Major Depression
WANGXu-dong, QIAOMing-qi, ZHANGZhang-jin, CHENGang, CHENJia-xu, TUYa
2016, 32(1): 93-96.
[Abstract](1050) [PDF 1074KB](863)
Abstract:
Disease burden caused by major depression ranks on the top. However， current mainstream western medicine with monoamine-based antidepressants has remarkable limitations， in contrast to the unique efficacy of traditional Chinese medicine (TCM). Here we reviewed the recent progress in TCM treatment of the disorder， including clinical outcomes， rapid onset of antidepressant response， treatment based on pattern identification of major depression， novel neuropharmacological mechanisms， and antidepressant effects of acupuncture. The clinical and basic research all supported the antidepressant efficacy of TCM， and some with rapid onset. It is suggested that the future research should address the issues of the standardization of treatment based on pattern identification， disease prevention and treatment outcomes using large population， and emphasize the basic research employing systems biology and multidisciplinary collaborative approaches.
Study on the Theory of "heterogeneous restriction" from the Material Base of Detoxicity Effect of the Compound Prescription of Chinese Herbal Medicine
LU Yan, XIE Tong, LI Huan, ZHOU Xue-ping
2016, 32(1): 97-100.
[Abstract](635) [PDF 1057KB](712)
Abstract:
The "Heterogeneous Restriction” theory comes from the clinical， which is the innovation of the traditional Chinese medicine theory， plays an important role in guiding the clinical application of toxic Chinese medicine. Under the guidance of "Heterogeneous Restriction” theory， in order to clarify the inherent mechanism of the compatibility of Chinese herbal medicine compound prescription， we must clarify the material base of detoxicity effect. Study on the mechanism of detoxicity effect on the basis of the compound prescription of Chinese herbal medicine， we should focus on three approaches: the toxic chemical constituents， the metabolism of toxic components， the correlation between toxic substances and effects. To explore in this field， we must .fully use the latest technology and research methods of modern science， and strengthen a multidisciplinary collaboration.

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