南京中医药大学学报

Discussion on Professor Xia Guicheng's Academic Thought of Treating Amenorrhea by Regulating Heart

WANG Jing

2015, 31(5): 401-406.

[Abstract](1817) [PDF 475KB](2721)

Abstract:
Professor Xia Guicheng put great emphasis on the treating of amenorrhea by regulating heart. He thinks that heat is closely related to amenorrhea. Heart-kidney-uterine plays an important role in menses and its periodic changes. If the balance of heart and spleen is disturbed and the heart qi is blocked， then the heart qi will be stagnated and the uterine vessels will be blocked， which together will cause amenorrhea. According to menstrual cycle and cycle regulating theory， thought amenorrhea may have lasted for a long time， the patients are still in the period of postmenstrual， and they have imbalance of Yin zhang gui shui， or there are other factors together contribute to the severe Yin zhang and it has problem transferring into Yang. Therefore patients have problem having ovulation to expel the eggs. So the treating should focus on postmenstrual period. Tranquilizing mind is of great significance to the treatment. On the basis of yin enriching and kidney nourishing， drugs which can tranquilize the mind should be used and during the same time， doctors should care about patients' emotions and try to help them to be optimistic.

Study on Concept of Lasting Movement in Essentials from the Golden Cabinet

FU Xin-wei

2015, 31(5): 407-408.

[Abstract](937) [PDF 296KB](1142)

Abstract:
The concept of lasting movement permeates into many aspects of TCM， has a profound impact on the construction of TCM theoretical system and becomes the driving force of pushing forward the development of the discipline to guide the clinical practice. The concept in Essentials from the Golden Cabinet mainly manifests in disease transformation， disease diagnosis， therapy and medication. The principle is good for the comprehensive and accurate diagnosis of diseases and treatment based on syndrome differentiation so as to improve clinical efficacy.

The Enlightenment of Zhang Xichun's Academic Thoughts of Treating Heart Failure Related Syndromes

ZHU Xian-hui

2015, 31(5): 409-411.

[Abstract](912) [PDF 348KB](1609)

Abstract:
Heart failure is a serious outcome of many cardiac diseases. Medical thoughts like "Chuan wu shan zheng" and "Da qi xia xian" in Zhang Xichun's Records of Chinese Medicine with Reference to Western Medicine are related to the diagnosing and treatment of heart failure. Replenishing qi and promoting urination， taking Han syndrome into consideration， and preventive treatment of diseases are of great significance to modern clinical practices in treating heart failure.

Differential Serum Proteomic Analysis of Ankylosing Spondylitis Patients with Damp-heat Arthralgia Spasm Syndrome

SUNZhi-ling, XUXiao, WANGLing, WANGFu-qiang, XUZhi-yang, JIWei

2015, 31(5): 412-415.

[Abstract](917) [PDF 471KB](1237)

Abstract:
OBJECTIVE Proteomic technique was applied to analyze ankylosing spondylitis (AS) patients with damp-heat arthralgia spasm syndrome. METHODS Serum proteins of AS patients with damp-heat arthralgia spasm syndrome group (n=30) was compared with that of normal control group (n=30) and AS patients with cold-dampness arthralgia spasm syndrome group (n=30) respectively by two-dimensional gel electrophoresis; the common differential protein spots of damp-heat arthralgia spasm syndrome were analyzed by the second order MS. The validation of the differential protein was performed using Western blot. RESULTS Seven kinds of common differential protein spots were selected and five kinds of proteins were identified by mass spectrometry. They were fibulin 1， dipeptidy l， peptidase 1， kininogen 1， mutant IL 17F， mannose-binding protein C and related to extracellular matrix protein， protease， coagulation factor， cytokine， transport protein， and so on. Results of Western blot were consistent with the proteomic results. CONCLUSION These proteins are implicated in the pathogenesis and pathological processes of AS with damp-heat arthralgia spasm syndrome， and provide a basis for further studies of the essence of the syndrome in traditional Chinese medicine.

Influence of Acupuncture on Plasma 5-HT， NPY and CGRP in Patients with Constipation-predominant Irritable Bowel Syndrome

PEILi-xia, ZHULi, CHENLu, WUXiao-liang, ZHOUJun-ling, GENGHao, SUNJian-hua

2015, 31(5): 416-419.

[Abstract](991) [PDF 418KB](1217)

Abstract:
OBJECTIVE To explore the acupuncture treatment mechanism of constipation-predominant irritable bowel syndrome(IBS-C) from the perspective of brain-gut axis， by observing the plasma 5HT， CGRP and NPY level before and after the treatment. METHODS 60 patients with IBS-C were randomly divided into two groups. 30 cases in the acupuncture group(AG) were treated by acupuncture five times a week， with such acupoints as Tianshu， Zusanli， Shangjuxu， Taichong， Sanyinjiao， Yintang， Baihui being selected. The other 30 cases in the medicine group(MG) were treated with lactulose oral solution， 15 ml per time for three times a day. After 4 weeks treatment， enzyme-linked immunosorbent assay (ELISA) was used to detect the plasma 5HT， NPY and CGRP level of 30 healthy volunteers and the two treatment groups and observe the improvement of clinical symptoms. RESULTS ①Both groups could significantly improve the clinical symptoms in patients with IBS-C (P<0.01)，while the curative effect in the AG was superior to that in the MG (P<0.01). ②The levels of 5-HT， NPY and CGRP in patients with IBS-C of two treatment groups were significantly higher than those in healthy volunteers， and the difference was statistically significant (P<0.01). The plasma 5-HT， NPY and CGRP levels all went down in both groups after treatment， and the differences of 5-HT， NPY and CGRP levels were significant when compared to the levels before treatment in AG(P<0.01). The differences of 5-HT，NPY levels were also significant when compared to the levels before treatment in MG(P<0.01)， while there was no significant difference on CGRP(P<0.01). There were no significant differences of 5-HT and NPY levels between these two groups after treatment (P>0.05)， but the difference of CGRP was highly statistically significant(P<0.01). The downward trend of 5-HT，NPY and CGRP level was better in the AG. CONCLUSION ①Acupuncture treatment can significantly improve the clinical symptoms in patients with IBS-C， and the theraputic effect is superior to that in the MG. ②The increase of 5-HT， NPY and CGRP level in patients with IBS-C after treatment suggests a close relationship between the abnormal level of brain-gut peptide and IBS syndrome. ③Acupuncture treatment can decrease the plasma levels of 5-HT， NPY and CGRP， alleviate the degree of abdominal pain and discomfort， which indicates that benign regulation of brain-gut peptide level in patients with IBS-C by acupuncture may be one of the effective mechanisms in treatmenting this disease.

Clinical Observation on Preventive Effect of Oxiliplatin Induced Peripheral Neuropathy by Fumigation of Siteng Yixian Decoction

JIANGJing-wen, CHENXue-wu, CAIHong-bing, WANGLin, FUYing-jin, LUORong-cheng

2015, 31(5): 420-423.

[Abstract](1118) [PDF 408KB](1081)

Abstract:
OBJECTIVE To observe the preventive effect of oxaliplatin induced peripheral neuropathy treated by fumigation of Siteng Yixian decoction. METHODS Totally 150 colorectal cancer patients from oncology department Of Hainan provincial hospital of TCM between January 2010 and December 2014 were enrolled in this study. And they were randomized into two groups， with 75 cases in each. The patients in the control group only received oxaliplatin based chemotherapy. While the patients in the treatment group were given the chemotherapy combined with fumigation of Siteng Yixian decoction. The fumigation lasted for forty minutes a time， twice a day. After the treatment of two cycles chemotherapy， the incidence rate and severity classification of peripheral neuropathy was analyzed one month later to evaluate the preventive effect. RESULTS The difference of incidence rate of peripheral neuropathy was significant， with the rate being 18.7% in the treatment group and 56.0% in the control group(P<0.01). The severity classification was evidently higher in the control group(90.37) than those in the treatment group(60.63)， and there exised a statistical significance(P<0.05). Among the patients who had second degree peripheral neuropathy or above， the oxaliplatin accumulative dose was (1 054.76±124.6) in the treatment group and (823.47±190.67) in the control group， and the difference was statistically significant(P<0.05). Logistic regression indicated that fumigation of Siteng Yixian decoction was the protective factor for the oxaliplatin-induced peripheral neuropathy. CONCLUSION Fumigation of Siteng Yixian decoction can reduce the incidence rate， decline the severity classification of oxaliplatin-induced peripheral neuropathy and increase the oxaliplatin tolerance dose. It is the independent preventive factor for oxaliplatin-induced peripheral neuropathy.

The Effect of Huobahuagen Tablets in Intervening Diabetic Nephropathy in Clinical Stage and the Influence on the Level of Serum HGF

WANG Li-juan, YU Jiang-yi, LUO Mei, FAN Li-ping

2015, 31(5): 424-427.

[Abstract](1104) [PDF 378KB](940)

Abstract:
OBJECTIVE To observe the treating effect of Huobahuagen tablets in intervening diabetic nephropathy in clinical stage and the influence on the level of serum HGF. METHODS DN patients in clinical stage were divided into two groups: the treatment group(Huobahuagen tablets together with Irbesartan capsules) and the control group(Irbesartan capsules)， with 30 cases in each group. The control group was given Irbesartan capsule (150 mg) every day in the morning for once and the patients took it with an empty stomach. The treatment group， besides taking the same medicine as prescribed for the control group， was also given Huobahuagen tablets， 3 tablets a day for three times 0.5h after each meal. FPG， 2 hPG， HbA1c， TG， TC， LDL-C， HDL-C， blood SCr， urine ACR， UTP， HGF level were tested. RESULTS ACR， UTP， SBP， DBP， blood fat， and SCr in both groups decreased after the treatment (P<0.05~0.01) and the decrease in the treatment group was sharper than that in the control group (P<0.05~0.01). The total effective rate in the treatment group was better in the treatment group (83.3% vs 40%，P<0.01). HGF levels in both group increased after the treatment (P<0.01). HGF level in the treatment group showed more increase compared with that of the control group (P<0.01). CONCLUSION Huobahuagen tablets can effectively lower the level of blood creatinine and the rate of urinary albumin excretion， increase the level of serum HGF， and possibly slow down DN progression.

Research on Measuring Scale for Pathogen Unit of Hemorrhagic Stroke Due to Heat-Stasis

LIGuo-chun, ZHOUXue-ping, WANGJun-qin, GUOWei-feng, YEFang, WUMian-hua, JINMiao-wen

2015, 31(5): 428-432.

[Abstract](969) [PDF 393KB](1533)

Abstract:
OBJECTIVE To establish measuring scale for the pathogen unit of heat-stasis on hemorrhagic stroke and explore the objective， standard and quantitative method for measuring heat-stasis pathogenesis. METHODS Measuring questionnaire was designed according to the production process of standard scale， and heat-stasis information representation was selected through three rounds of expert consultation， so as to establish the entry pool of scale. Related metrical data of hemorrhagic stroke due to heat-stasis was collected on spots with the clinic epidemiology/DME principles for 4 times， and the credibility， validity and applicability of scale were assessed. RESULTS By applying the method of subjective evaluation and step screening based on importance evaluation， the original entry pool of scale concerning hemorrhagic stroke due to heat-stasis was established， which included 32 information representations， belonging to the dimension of blood heat， blood stasis and consciousness respectively. And the scale was further optimized after testing the clinical samples， with 25 items of higher credibility and validity were chosen. CONCLUSION We have preliminarily established a set of measuring tool for the pathogen unit of heat-stasis on hemorrhagic stroke， and the scale shows that consciousness change is an important representation in distinguishing hemorrhagic stroke due to heat-stasis from other diseases.

Clinical Application Research on Glycyrrhizae Prescriptions with Incompatible herbs and Association Analysis of Their Characteristics

ZUOTing, TIANShuo, LIYan, YANGFan, FANXin-sheng

2015, 31(5): 433-436.

[Abstract](914) [PDF 337KB](1121)

Abstract:
OBJECTIVE To explore the clinical application and features of Glycyrrhizae prescriptions with incompatible herbs by doing the survey and contrastive analysis in different third-grade class-A hospitals from the central and eastern parts of China. METHODS Clinical prescriptions containing Glycyrrhizae with incompatible herbs were collected. Association rules were utilized to analyze the compatible features of these herbs. RESULTS The drugs with the highest support in the two places are: liquorice root， seaweed， Indian bread， kelp， cassia twig， white peony root， tree peony bark， peach seed， common self-heal fruit-spike， starwort root etc. liquorice root and seaweed ranked the first in both places. The pairs of drugs highly recommended in the two places are: seaweed and liquorice root， liquorice root and Indian bread， seaweed and Indian bread， seaweed and kelp， liquorice root and kelp， liquorice root and cassia twig etc. The drug pair with the highest support in the east is liquorice root and seaweed， and then is the seaweed and kelp and liquorice root and kelp. Besides liquorice root and seaweed， the drug pair with the highest support in the middle part is liquorice root and Indian bread， and then are the Indian bread and seaweed， white peony root and liquorice root. The key pairs with high support in both places are: seaweed- liquorice root- Indian bread， seaweed-liquorice root-kelp， seaweed-liquorice root-cassia twig， liquorice root-Indian bread-cassia twig， seaweed-liquorice root- white peony root. The drug pairs with high support in the east are: liquorice root-seaweed-kelp， liquorice root-seaweed-Indian bread， liquorice root-chicken gizzard linning-seaweed， chicken gizzard linning-seaweed-Indian bread， liquorice root-chicken gizzard linning-Indian bread etc. The drug pairs with high support in the middle part are: Indian bread- liquorice root- seaweed， liquorice root- cassia twig- seaweed， white peony root- liquorice root-seaweed， Indian bread-liquorice root-cassia twig， Indian bread-tree peony bark-liquorice root etc. CONCLUSION The clinical application of Glycyrrhizae with incompatible herbs can be found， with the herbal pair of Thallus Sargassi and Glycyrrhizae being the most common one. But there exits the differences in the combined application between eastern China and central China.

Study on Detection and Quantification of Four Properties and Five Tastes of Drugs for Relieving Exterior Disorder by Electronic Nose and Electronic Tongue

SHENGLiang, CHENJian-wei, WANGGuang-yao, TANGDe-cai, WANGXing-hua, ZHANGYa, XULu-zhou

2015, 31(5): 437-444.

[Abstract](967) [PDF 1034KB](1087)

Abstract:
OBJECTIVE To detect and quantize four properties and five tastes of diaphoretic medicine with electronic nose and electronic tongue. METHODS 17 traditional Chinese medicine and 8 western medicine， a total of 25， were detected by electronic nose. The similarities and differences of PCA and radar map were analyzed and the bitterness value of some drugs by electronic tongue， which provided a quantitative basis for determination. RESULTS The PCA， radar map and bitterness value of diaphoretic medicine displayed similarities and differences with western medicine， which accorded with the smell. CONCLUSION Electronic nose and electronic tongue can be used to detect and quantize four properties and five tastes of Chinese and western medicine.

Preparation and Evaluation of Oleanolic Acidsolid Dispersion

WANGJing, YANHong-mei, DONGYu

2015, 31(5): 445-448.

[Abstract](913) [PDF 574KB](1023)

Abstract:
OBJECTIVE To prepare oleanolic acid-nano calcium carbonates solid dispersion and to enhance its in vitro release. METHODS Oleanolic acid-nano calcium carbonate solid dispersion was prepared by using solvent evaporation method. By using scanning electron microscopy (SEM)， differential scanning calorimetry (DSC) and X-ray powder diffraction (X-RD)， the Microscopic structure and physicochemical properties of solid dispersion were analyzed. And its in vitro release also was investigated. RESULTS It was suggested that oleanolic acid may be present in solid dispersion as amorphous substance. The in vitro release determination results of oleanolic acid -nano calcium carbonate (1∶2) solid dispersion showed accumulated dissolution rate of oleanolic acid was up to 83.30% at 60 min which was much higher than oleanolic acid bulk drug. CONCLUSION The results indicated that in vitro dissolution of oleanolic acid was improved greatly by the solid dispersion with nano calcium carbonate as a carrier.

Rapid Determination of Bornyl Acetate in Amomum Villosum by Near-Infrared Spectroscopy with Partial Least Square

FANMing-yue, BAIYan, LEIJing-wei, XIECai-xia, HAOMin

2015, 31(5): 449-452.

[Abstract](1036) [PDF 501KB](1352)

Abstract:
OBJECTIVE To rapidly determine the content of bornyl acetate in amomum villosum by near-infrared spectroscopy (NIRS). METHODS The content of bornyl acetate in 101 amomum villosum was determined by GC. The quantitative analysis model of bornyl acetate in amomum villosum was established by collecting near-infrared spectra combined with partial least squares (PLS). RESULTS The correlation coefficients (R²) ， the root-mean-square error of calibration (RMSEC) and the root-mean-square error of cross-validation (RMSECV) of the quantitative calibration model of bornyl acetate were 0.992 59， 0.014 5 and 0.071 4; the root-mean-square error of prediction (RMSEP) was 0.016 7. CONCLUSION NIRS is simple and accurate， which can be used for rapid determination of bornyl acetate in amomum villosum.

Evaluation of Salt Processing on Pharmacokinetics of Geniposidic Acid in Eucommia ulmoides Oliver

GAOQian-qian, WENGZe-bin, ZHAOGen-hua, YANCui-ping, CHENZhi-peng, CAIBao-chang, XUHan, LIWei-dong

2015, 31(5): 453-456.

[Abstract](987) [PDF 430KB](1096)

Abstract:
OBJECTIVE To establish UHPLC-MS/MS method to determine geniposidic acid in rat plasma and to investigate the effect of salt processing on pharmacokinetics of geniposidic acid in Eucommia ulmoides Oliver. METHODS Chromatographic column: BEH-C₁₈（100 mm×2.1 mm， 1.7 μm); the mobile phase: acetonitrile-0.1% formic acid aqueous solution. The gradient elution program was performed at a flow rate of 0.3 mL/min. RESULTS The main pharmacokinetic parameters of geniposidic acid in raw and salt products were that：AUC_(0-t) were （1 47.18±272.28），（2 120.694±664.532）ng·h/mL；AUC_(0-∞) were （1 564.42±273.97），（2 145.61±659.983）ng·h/mL；C_max were （517.59±51.24）， (733.292±261.34)ng/mL；MRT were （2.68±0.11），（2.551±0.08）h；T_1/2 were （1.37±0.08），（1.43±0.17） h；T_max were （1.52±0.1），（1.51±0.1）h. CONCLUSION Salt processing can obviously enhance the absorption of geniposidic acid in Eucommia ulmoides Oliver， and increase the bioavailability.

Growth suitability of Magnolia officinalis based on habitat suitability

YANGHong-bing, MINGMeng-die, ZHANGCheng-cheng, GUOLan-ping, HUANGLu-qi, ZHUShou-dong, SHILei

2015, 31(5): 457-460.

[Abstract](972) [PDF 452KB](1000)

Abstract:
OBJECTIVE To study the growth suitability of Magnolia officinalis on the basis of investigation on the correlation between its growth and ecological factors， and to provide scientific basis for determining the suitable planting region of Magnolia officinalis in China. METHODS Based on the investigation of 43 sampling points of Magnolia officinalis， the ecological factors affecting its growth was calculated by Maxent. The geographic information system(GIS) was applied to assess the growth suitability rank dividing of Magnolia officinalis in China. RESULTS Precipitation of October， altitude， September precipitation， precipitation in July is the main ecological factors that influencing the growth of Magnolia officinalis. The habitat suitability values of Magnolia officinalis in Southwest of Hubei province and Eastern Chongqing is highest. CONCLUSION Hubei and Chongqing are the suitable planting areas of Magnoliae officinalis.

Antioxidant Effects of Buxin Ruanmai Granules Serum on AngⅡ-induced Human Umbilical Vein Endothelial Cells

YANDong, QIANYu-liang, YANShi-hai, WANGNing, ZHOUChao-wei, TANGShu-hua

2015, 31(5): 461-464.

[Abstract](1070) [PDF 438KB](968)

Abstract:
OBJECTIVE To observe the effects of Buxin Ruanmai granules (BXRM) serum on AngiotensinⅡ（AngⅡ）-induced oxidative damage related indicators， such as SOD，NADPH ，MDA，p40^phox，p47^phox and p67^phox， in HUVEC. METHODS Rats were randomly divided into normal group，Valsartan group and BXRM group，each group of 10， and lavaged for 6 weeks. Drug serum were prepared from arterial blood. Then 10^-6 mol/L Ang Ⅱ induced HUVEC damage and apoptosis model of human umbilical vein endothelial cells was established， and drug serum was used to intervene. SOD were determined by xanthinoxidase. NADPH was determined by ELISA. MDA was determined by thibabituric acid method. The expression of p40^phox， p47^phox and p67^phox in cell membrane and cytoplasm of HUVEC were determined by Western blot． RESULTS After HUVECs were damaged by 10^-6 mol/L Ang Ⅱ，the content of SOD decreased ， NADPH and MDA increased ， and p40^phox， p47^phox and p67^phox transferred to the membrane of HUVECs. However， the content of SOD increased， NADPH and MDA decreased， and p40^phox， p47^phox and p67^phox were inhibited to transfer to the membrane， after the medication of BXRM serum. CONCLUSION BXRM serum can inhibited the oxidation of AngⅡ induced HUVEC damage and prevent arteriosclerosis.

Effect of Active Components from Lycii Cortex on Proliferation of High Glucose-induced Glomerular Mesangial Cells and Extracellular Matrix

ZHOU Ying, PI Wen-xia, CAI Bao-chang, ZHAO Wen-wang, HUANG Pu-jun

2015, 31(5): 465-468.

[Abstract](1051) [PDF 420KB](1470)

Abstract:
OBJECTIVE To observe the effect of active components from Lycii cortex on the proliferation of high glucose-induced glomerular mesangial cells (GMCs) and the secretion of extracellular matrix (ECM). To investigate the protective mechanism of diabetic nephropathy. METHODS GMCs were cultured and divided into high glucose group (final glucose concentration of 30 mmol/L)， blank group (final glucose concentration of 5.5 mmol/L)， betaine and kaempferol groups of 5 different concentrations of 0.01， 0.1， 1， 10， 100 μmol/L. The proliferation of high glucose-induced GMCs were observed by MTT method and secretion of ECM was detected by ELISA assay. RESULTS Different doses of betaine and kaempferol inhibited the proliferation of GMCs， and decreased the content of ECM， compared with high glucose group （P<0.05~0.01）. CONCLUSION Betaine and kaempferol can protect the high glucose-induced GMCs through inhibiting the proliferation of GMCs and decreasing the content of ECM.

San Huang Decoction Promotes Apoptosis of Breast Cancer Cells Through Regulating Aurora Kinase A

XUYan-lei, CHENXi-yan, CHENXu, BIANWei-he, YAOChang, ZHUXiao-shu, CHENJia-jing

2015, 31(5): 469-474.

[Abstract](945) [PDF 9109KB](1457)

Abstract:
OBJECTIVE To explore the effect of San Huang decoction on the apoptosis of breast cancer cells and the effect on the mRNA and protein expression and function of Aurora kinase A and discuss the underlying mechanism of San Huang induced apoptosis. METHODS The inhibition of breast cancer cells proliferation was determined by CCK-8 assay. The apoptosis of breast cancer cells was detected by AnnexinV-FITC/PI Staining. The expression of mRNA of Aurora A was examined by q-PCR analysis. The expression of apoptosis-related proteins and Aurora A were determined by Western Blot analysis. RESULTS San Huang decoction inhibited the proliferation of breast cancer cells in a does-dependent manner(P<0.05). The effect of inhibition caused by San Huang decoction 48 hours after delivering to breast cancer cells was better than 24 hours(P<0.05) ， although similar as 72 hours(P>0.05). San Huang decoction was also found to induce apoptosis in both MCF-7 and MDA-MB-231 cell lines in a dose-dependent manner. Consistent with cellular results， San Huang decoction treatment significantly increased the apoptosis-related protein level of cleaved-PARP(c-PARP)， cleaved-Caspase 3(c-Caspase 3) and Bax， down-regulated Bcl-2 in a does-dependent manner. Meanwhile， San Huang decoction decreased the mRNA and protein level of Aurora A and increased those of p53 in a does-dependent manner. CONCLUSION San Huang decoction at the first time was able to promote the apoptosis of breast cancer cells via inducing the suppression of Aurora A.

Effects of Electroacupuncture at Back-shu and Front-mu Points on Expression of GDNF in Colon Tissue of Functional Constipation Mice

ZHANG Wei, LI Ying, LIU Li-sha, LUO Fang-li, ZHENG Qian-hua

2015, 31(5): 475-479.

[Abstract](1001) [PDF 1306KB](1257)

Abstract:
OBJECTIVE To explore the mechanism of electroacupuncture at Back-shu and Front-mu points for functional constipation treatment. METHODS 32 mice were randomly divided into control group， model group， electroacupuncture (EA) group and EGC group. Compound diphenoxylate were gavaged for modeling， and electroacupuncture Tianshu and Dachangshu points， 30 min each time for a total of 5 times treatment. By light microscopy， electron microscopy， immunohistochemistry and in situ hybridization， the morphology of colonic epithelial structure were observed， and the level of GDNF protein and mRNA expression in colon were detected. RESULTS The expression of GDNF protein and mRNA in model group was significantly decreased (P<0.01). Electroacupuncture at back-Shu and front-Mu points improved the expression of GDNF protein and mRNA (P<0.05)， and light microscope and electron microscope showed that acupuncture repaired damaged depithelial cells， and then improved the intestinal transmission function. The expression of GDNF protein in EGC group showed no significant difference with the model group (P>0.05)， but significantly decreased， compared with EA group (P<0.05). GDNF mRNA was significantly higher than model group (P<0.05)， but lower than EA group with no significant difference (P>0.05). The morphology change of colon tissue is more serious. CONCLUSION Electroacupuncture at back-Shu and front-Mu points can improve the expression of GDNF protein and mRNA in EGC cells， repair the colonic epithelial cells， and then improve the intestinal transmission function.

Treating of Ulcerative Colitis by Professor Zhang Xiaoping from the Perspective of Qihua of Spleen and Stomach

HELing, LILong-hua

2015, 31(5): 480-482.

[Abstract](995) [PDF 356KB](1239)

Abstract:
This paper is about the clinical experience of Professor Zhang Xiaoping in treating ulcerative colitis from the perspective of Qihua of spleen and stomach. Professor Zhang thinks that the pathogenesis of the disease is root deficiency and branch excess. Root deficiency refers to the spleen deficiency and branch excess refers to damp-heat， epidemic toxin， and cold and damp invading intestines and fu organs. The occur and development of the disease are related to the disorder of digesting， imbalance of dryness and dampness， and abnormal movement of qi. In clinical practices， methods like regulating the rising and descending of qi， clearing away the internal evils， regulating the inputting and outputting， defending from the external evils， caring about the digesting， and restoring the function of spleen and stomach， regulating the balance of dryness and dampness， and strengthen the function of intestines and fu organs.

Clinical Experience of Director Gao Jiansheng Treating Macular Edema

XU Huang-jie, YANG Wei, SONG Jian-tao, WU Zheng-zheng

2015, 31(5): 483-484.

[Abstract](1011) [PDF 301KB](1327)

Abstract:
The clinical experience of director Gao Jiansheng treating macular edema caused by diabetic retinopathy and retinal vein occlusion is introduced. This paper takes the view that the etiology and pathogenesis of the edema induced by both is deficiency of both qi and yin with the syndrome of blood stasis and qi stagnation of ocular collateral and puts forward the modified therapy with Mimeng Flower decoction.

Z-poke Needle in the Treatment of Periarthritis of Shoulder: A Study of 64 Cases

SUNQiang, FENGDa-gang, FANXiao-hong

2015, 31(5): 485-487.

[Abstract](950) [PDF 351KB](1095)

Abstract:
OBJECTIVE To evaluate the clinical effect of Z-poke needle in the treatment of periarthritis of shoulder. METHODS A total of 128 patients with periarthritis of shoulder were randomly divided into 2 groups， with 64 cases in each group. Z-poke needle therapy was performed in the treatment group. Electroacupuncture therapy was conducted in the control group. After the treatment， the patients of two groups were guided to do the functional exercise of shoulder joint such as lift， extension， adduction， abduction， etc， in order to evaluate the clinical curative effect before and after the treatment. RESULTS After 2 courses of treatment， the functional level of shoulder joint significantly improved after the treatment(P<0.01)， and the joint mobility of the treatment group improved more obviously than that of the control group(P<0.01). As for VAS score， the pain score of the treatment group decreased more significantly compared with that of the control group(P<0.01)， and the UCLA score of the treatment group rose higher than that of the control group（P<0.05）， and the difference was statistically significant. The total effective rate of the treatment group (93.75%) was significantly higher than that of the control group (84.38%)，P<0.05.CONCLUSION The method of Z-poke needle has definite curative effects on periarthritis of shoulder with simple manipulation and should be widely applied to clinical practice.

Bailemian Capsule in Treating Insomnia: A Study of 85 Cases

ZHANGDong, YUFeng-chun, LUOBin, CAOWei

2015, 31(5): 488-490.

[Abstract](5076) [PDF 328KB](3815)

Abstract:
OBJECTIVE To observe the treating effect of Bailemian capsule to insomnia patients with emotional disorder. METHODS Patients were divided into three groups: Bailemian capsule group(85 cases)， Estazolam group(68 cases) and bond group(Bailemian capsule together with Estazolam)(107 cases). AIS and ESS were used to assess the severity of insomnia and daytime sleepiness of patients before and after the treatment. SAS and SDS self-rating anxiety scales were used to assess the emotion of patients before and after the treatment. RESULTS After the treatment， AIS and ESS scores of three groups decreased significantly(P<0.01). SAS and SDS scores also decreased(P<0.01). CONCLUSION Bailemian capsule can relieve the symptoms of insomnia and daytime sleepiness and relieve anxiety and depression. The treating effect of Bailemian capsule and that of the bond group were similar， but better than that of the single using of Estazolam.

Erdi Biejia Decoction together with Tadalafil in Treating Diabetic Erectile Dysfunction: A Study of 35 Cases

ZHOU Hua, SUN Zhi-xing, XIANG Zi-liang

2015, 31(5): 491-493.

[Abstract](922) [PDF 350KB](1365)

Abstract:
OBJECTIVE To discuss the clinical effect of Erdi Biejia decoction together with low dose of Tadalafil in treating diabetic erectile dysfunction. METHODS 70 patients with DED were randomly divided into two groups: the control group(35 cases) and the treatment group(35 cases). The treatment group was given Erdi Biejia decoction one dosage every day， orally taken in the morning and evening. Tadalafil pills(5mg) were orally taken once every night for eight weeks. The control group took Tadalafil pills(5mg) once every night for eight weeks. Before and after the treatment， IIEF-5 scores and nocturnal penile tumescence were recorded. RESULTS After the treatment， IIEF-5 scores and nocturnal penile tumescence of both groups significantly improved(P<0.01). After the treatment， the treatment group performed better than the control group(P<0.05). So serious side effects showed in the two groups. CONCLUSION Erdi Biejia decoction together with low dose of Tadalafil were effective in treating DED and were worth of being widely applied in clinical practices.

Effects of Andrographolide on Expression of SREBPs and Lipid Metabolism in HepG2 Cells

CHEN Yu, LUO Hao

2015, 31(5): 494-497.

[Abstract](903) [PDF 520KB](1416)

Abstract:
OBJECTIVE To explore the effects of Andrographolide on expression of SREBPs and lipid metabolism in HepG2 cells， and to study the possible mechanism. METHODS Cell viability was evaluated by Cell Counting Kit-8; Dual luciferase reporter gene assay was used to test the transcription activity of SREBP; The SREBPs target genes expressions were measured by Quantitative Real-Time PCR; The intracellular triglyceride (TG) and total cholestero l(TC) contents were measured by using commercially available test kits. RESULTS Andrographolide in 0~80 μmol/L concentration range has no effect on HepG2 cell activity; Andrographolide could markly inhibited human SRE promoter activity in a dose-dependent manner， and the mRNA level of SREBPs target genes were significantly downregulated by Andrographolide at the dose of 35 μmol/L. In addition， contents of intracellular cholesterol and triglyceride were significantly suppressed by Andrographolide treatment. CONCLUSION These results suggested that Andrographolide might improve lipid metabolism through downregulating the mRNA levels of SREBPs target genes.

The Preparation of Inclusion Compounds of Resveratrol with β-cyclodextrin

CHEN Hong-xuan, LI Kun, WANG Ze-xia, YANG Xiao-yun, ZHANG Cui-li

2015, 31(5): 498-500.

[Abstract](900) [PDF 403KB](1174)

Abstract:
OBJECTIVE To study the optimum preparation condition of inclusion complexes of resveratrol by ultrasonic method. METHODS The inclusion complexes were prepared by ultrasonic method. The optimum preparation condition was studied by orthogonal test with encapsulation rate as the index of evaluation. RESULTS The optimum preparation condition：the ratio of resveratrol to β-CD is 1∶2，inclusion temperature is 50℃，ultrasonic time is 4 hours，ultrasonic power was 300W. The inclusion of inclusion rate is 44.58%. CONCLUSION The inclusion complexes prepared by ultrasonic method have such advantages:simplicity of operator， condition easily controled，slightly affected by external influence.

2015 Vol. 31, No. 5

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