南京中医药大学学报

Study of Ding Zeming's Academic Idea of Syndrome Differentiation and Treatment of Hemorrhoids Bleeding

DING Shu-qing, DING Yi-jiang, HUANG-FU Shao-hua, LU Ting

2015, 31(2): 101-103.

[Abstract](1033) [PDF 379KB](1185)

Abstract:
To summarize the syndrome differentiation and treatment of hemorrhoids bleeding of doctor Ding Zeming by analyzing the composing principle of traditional Chinese medicines(Hemorrhoid Bleeding Mixture for oral administration， Yuhuai Zanglian pill， Buqi Shexue decoction and Fanhuang Xiaozhi liquid for external use and injection， etc)， which are developed by doctor Ding and are used to treat bleeding from hemorrhoids. The pathogenesis of bleeding form hemorrhoids are the oppression from wind， heat(fire) and dryness to the large intestine， the obstruction of the collaterals， the blood overflowing the veins and are mostly excess syndrome. The treating methods are cooling the blood， clearing away the heat， treating both symptoms and root causes， using charcoal drug， treating the internal and external symptoms， combining diseases and syndromes to find the common causes.

Discussion on Professor Zhou Zhongying's Treating of Complicated Diseases from the Pathogenesis of Kidney Deficiency and Liver Hyperactivity

ZHANG Shi-an, YE Fang

2015, 31(2): 104-107.

[Abstract](1034) [PDF 415KB](1361)

Abstract:
Based on the relation between liver and kidney， this paper discussed Professor Zhou Zhongying's theory of kidney deficiency and liver hyperactivity and its bases of disease and the clinical manifestations. Cases were shown to prove that the new terminology regarding to the pathogenesis as kidney deficiency and liver hyperactivity summarized the clinical manifestations of the relation about the diseases between liver and kidney and was of great significance to the clinical practices.

The Features and Clinical Application of Wang Xugao's Treating Methods for Dysphagia and Regurgitation

ZHANG Cheng-peng, SUN Yi-na, DAI Tian-mu

2015, 31(2): 108-109.

[Abstract](887) [PDF 314KB](1058)

Abstract:
This paper analyzed the cases regarding to dysphagia and regurgitation and discussed the treating methods in Wang Xugao's Cases. Doctor Wang combined Zhang Zhongjing's method of resolving the phlegm to tranquilize the reverse and Ye Tianshi's method of nourishing Yin and the stomach and meanwhile took the treating of three energizers and the treating of the mind and body into consideration. Dysphagia， regurgitation and gastroesophageal reflux disease have lots in common. While the syndrome differentiation and treatment of dysphagia and regurgitation were reviewed， it's of great significance for the differentiation and treatment of gastroesphageal reflux disease.

Shuzheng Granules in Treating Patients with Diabetic Foot after Interventional Therapy

CAOMing-man, LYUXiong, LUORen, JIANGDan, TANGHui, MOWei

2015, 31(2): 110-113.

[Abstract](1029) [PDF 385KB](953)

Abstract:
OBJECTIVE To investigate the effect of Shuzheng granules on diabetic foot pain， ulcer healing and lower limb blood supply of the patients with diabetic foot after taking interventional treatment. METHODS Patients with diabetic foot who had taken interventional therapy successfully were randomly divided into Shuzheng granules treatment group (n=22) and basic treatment group (n=21). The two groups were given the same regular basic treatment while Shuzheng granules was added to the first group. Then the results including pain symptoms integral， ulcer healing extent， ankle-brachial index (ABI)， arterial flow velocity and the artery diameter before and after the treatment of the two groups were compared.RESULTS The improvement in pain symptoms integral depth of ulcer， ulcer area， ankle-brachial index， arterial blood flow velocity， arterial diameter and hemarheology were better in the treatment group (P<0.05).CONCLUSION ShuZheng granules combined with basic therapy has advantages in ulcer healing， pain relieving and lower limb blood flow increasing for patients with diabetic foot after intervention.

Clinical Observation on the Treatment of Chronic Secretory Otitis Media by Nasal Endoscope Assisted Salpingocatheterism Combined with Chinese Medicine Liquid Irrigation

ZHONG Lun-kun, SUN Yong-dong, CHEN Long-hui, ZHOU Xing-wei, FAN Jia, HE Teng

2015, 31(2): 114-117.

[Abstract](1201) [PDF 413KB](1163)

Abstract:
OBJECTIVE To study the clinical effect of Salpingocatheterism combined with Chinese medicine liquid irrigation under endoscope for chronic secretory otitis media (CSOM). METHODS From September 2012 to March 2014， 80 patients with CSOM(120 ears) in the clinic were randomly divided into two groups. The experimental group：40 cases(61ears) were treated with Salpingocatheterism combined with Chinese medicine liquid irrigation and the liquid named Qingqiao tang(empirical recipe) was injected for 4 to 7days with 2ml each time. The control group：40 cases(59ears) were treated by myingotomy with grommet insertion. The ventilation tube was generally pulled out after 6 to 8 weeks or dropped out by itself .All patients were followed up for 6 months and the effects were to be evaluated. RESULTS In the experimental group，among 61ears， 50 ears were cured，6 showed effectual results， 2 were positive to the treatment and 3 showed ineffective results. The cure rate was 81.97% and the total effective rate was 95.08% .In the control group，among 59 ears， 38 ears were recovered， 6 showed effectual results， 5 were positive to the treatment，10 showed ineffective results. The cure rate was 64.61% and the total effective rate was 83.05% . The therapeutic effect of experimental group was better than that of the control group. The complication rate of the experimental group was 9.84%，while the control group was 28.81%. The complication rate of the experimental group was significantly lower than that of the control group. The recurrence rate of the experimental group was 6.56% while the control group was 20.34%， the recurrence rate in the control group was significantly higher than that of the experimental group. The differences both had statistical significance (P<0.05). CONCLUSION The treatment of CSOM with salingocatheterism combined with Qingqiao tang irrigation under endoscope is effective and causes little injury. With a low recurring rate and less adverse reaction， this treating method is worth of being applied widely.

Clinical Study on Needling Method of Purging Liver and Tonifying Lungs in Treating Taiyin Cases with Depression

SHIYu, WANGZhi-xiang, ZHOUZhe-yi, LUChang-jun

2015, 31(2): 118-121.

[Abstract](1074) [PDF 398KB](1408)

Abstract:
OBJECTIVE To observe the clinical effects of needling method of purging liver and tonifying lungs in treating people of Taiyin constitution with depression and explore its endocrine and immune mechanism. METHODS Ninety cases with depression were randomly divided into 3 groups (treatment group， control group and medicine group)， with 30 cases in each group. The treatment group were treated by needling method of purging liver and nourishing lungs which originated from Korean medicine， and control group were treated by acupuncture of traditional Chinese medicine around Baihui (GV 20) and Neiguan(PC 6); the medicine control group were given Proza. And the treatment last 8 weeks. Then Hamilton Depression Scale (HAMD)， levels of CORT， ACTH， IL-6 and CRP in peripheral blood were compared in three groups both before and after the treatment. RESULTS After treatment， level of HAMD decreased in three groups (P<0.01)， with treatment group being the best (P<0.05~0.01); and levels of CORT and ACTH decreased dramatically in all groups (P<0.01)， with the treatment group being the most obvious (P<0.05~0.01); levels of IL-6 and CRP in peripheral blood reduced in all groups (P<0.01)， but there was no difference between them. CONCLUSION Compared to acupuncture of traditional Chinese medicine and Proza， needling method of purging liver and tonifying lungs can greatly improve depression and is effective in reducing levels of CORT， ACTH， IL-6 and CRP in peripheral blood of Tayin people with depression.

Clinical Observation on Neuromuscular Electrical Stimulation Combined with Acupuncture and Rehabilitation Exercise for Relieving Dysphagia in Patients with Craniocerebral Injury

WANG Hai-ming, CAI Chen, ZHANG Zhi-fang

2015, 31(2): 122-125.

[Abstract](874) [PDF 377KB](1002)

Abstract:
OBJECTIVE To explore the clinical effects of neuromuscular electrical stimulation combined with swallowing training for relieving dysphagia in patients with craniocerebral injury， providing reference basis for further study. METHODS 90 patients with craniocerebral injury suffered from dysphagia were selected and divided into A， B， C groups. Patients in group A received conventional treatment and rehabilitation exercise. Patients in group B were also given neuromuscular electrical stimulation and combined all the treatment in group A. Neuromuscular electrical stimulation and moxibustion therapy were added for patients in group C. The swallowing function， dysphagia degree and the quality of daily life were compared before and after the treatment. RESULTS The total effective rate in the three groups was 93.3%， 73.3% and 53.3% respectively with statistically significant differences (P<0.05). The VFG scores after treatment were higher than those before treatment in all the groups， showing statistically significant differences (P<0.05). The WST scores after treatment were lower than those before treatment in the three groups with statistically significant differences (P<0.05). The VFG score of group C were higher than that of group A and group B after treatment， which showed statistically significant differences (P<0.05). The WST score of group C were lower than that of group A and group B after treatment， indicating statistically significant differences (P<0.05). The SWAL-QOL scores after treatment were lower than those before treatment in all the groups with statistically significant differences (P<0.05). The SWAL-QOL score in group C were lower than that of both group A and B after treatment， which showed statistically significant differences (P<0.05). CONCLUSION Neuromuscular electrical stimulation combined with acupuncture and rehabilitation exercise can significantly improve patients' swallowing function and quality of life.

Effect of QiangXinKang on BNP and RDW Level of patients with Heart Failure with Reduced LVEF

CAI Shao-hang, WU Yi-ping, CHEN Hui, LIU Xue-na

2015, 31(2): 126-128.

[Abstract](993) [PDF 359KB](1297)

Abstract:
OBJECTIVE This paper is to discuss possible targets in the treatment of heart failure with reduced left ventricular ejection fraction (HFREF) with QiangXinKang by observing the effect of QiangXinKang on the brain natriuretic peptide (BNP) and red blood cell volume distribution width (RDW) of patients with HFREF. METHODS 198 patients meeting the selecting criteria of HFREF were randomly divided into the experimental group with 102 cases and the control group with 96 cases. Both groups received routine treatments conforming to Guideline for the Diagnosis and Treatment for Heart Failure (2007 Edition) and the experimental group took QiangXinKang additionally. After 4-week treatment， changes of BNP， RDW level and LVEF were compared between the two groups before and after treatment. RESULTS As LVEF was improved after treatment， BNP concentration and RDW level of patients in both groups were significantly reduced， while those of the experimental group decreased more significantly， which showed a significant difference between the two groups (P<0.05). CONCLUSION QiangXinKang may significantly reduce the BNP and RDW level and improve the heart function of patients with HFREF.

The Clinical Efficacy of Danlou Tablet in Non-revascularization Patients With Acute Coronary Syndrome and Phlegm and Blood Stasis Syndrome Type

XIAN Yu-qiong, LI Yan-song, XIA Jing-wen, JIANG Bing, JIANG Nian-xin, WEI Yong, YANG Wei

2015, 31(2): 129-133.

[Abstract](1116) [PDF 426KB](1132)

Abstract:
OBJECTIVE To investigate the clinical efficacy of Danlou tablet in Non-revascularization Patients with acute coronary syndrome (ACS)and phlegm and blood stasis syndrome type(PBSST). METHODS 70 ACS patients with PBSST for various reasons failed revascularization therapy were randomly assigned to the control group and the treatment group. The control group was given a standardized western medicine treatment and the treatment group was given Danlou tablets (every time 1.5g， 3 times a day) based on standardized western medicine treatment for a month. Angina episodes (intensity， frequency， duration)， nitroglycerin use (consumption， angina relief time after taking nitroglycerin ) and the score of angina， traditional Chinese Medicine（TCM）clinical syndrome (including: chest pain or tightness and its frequency and duration， palpitations， shortness of breath， fatigue and total syndrome score)， lipids including total cholesterol (TC)， low-density lipoprotein cholesterol (LDL-C)， high density lipoprotein cholesterol (HDL-C)， triglycerides (TG)] and hypersensitive C-reactive protein (hs-CRP) were observed in the two groups of patients before and after treatment. The clinical efficacy of angina，the score of TCM clinical syndrome and blood biochemical changes were recorded and compared between the two groups. RESULT The total effective rate of angina (85.29%) was significantly better than that of the control group (48.48%)(P<0.01). Compared with the same group before treatment， the frequency and duration of angina， the angina relief time after taking nitroglycerin， the amount of nitroglycerin， the score of angina and TCM clinical syndromes and the total score of syndrome，all these were significantly lower (P<0.01) in the two groups of patients after treatment. Compared with the control group， the frequency and duration of angina， the angina relief time after taking nitroglycerin， the amount of nitroglycerin， the total score of TCM clinical syndrome (P<0.05) and the score of angina (P<0.01) were significantly lower in the treatment group after treatment. The improvement of TCM clinical syndromes in the treatment group (the total efficiency of syndrome score was 79.41%) was significantly better than the control group (the total efficiency of syndrome score was 45.45%) (P<0.01). TC， LDL-C(P<0.01) and hs-CRP (P<0.05) levels after treatment were significantly lower than before. After treatment， hs-CRP in the treatment group was significantly lower compared with the control group(P<0.05). CONCLUSION Danlou tablet can promote a significant improvement to angina and TCM clinical syndromes in Non-revascularization Patients with ACS and PBSST， inhibit the inflammation， and show plaque stabilizing effects.

The Anti-hyperglycemic Activity of Crude Polysaccharides from Mactra Veneriformis in Alloxan Induced Diabetes in Mice

DONG Wen-nan, CHAI Yao, LIU Rui, CHENG Jian-ming, WU Hao

2015, 31(2): 134-137.

[Abstract](1022) [PDF 391KB](1326)

Abstract:
OBJECTIVE To study the anti-hyperglycemic effective sites of crude polysaccharides from Mactra veneriformis (MVPS). METHODS Hyperglycemia models were established by intravenous injection of alloxan via tail vein of ICR mice under a dose of 80 mg/kg. After 96 h， mice with fasting blood glucose levels≥11.1 mmol/L were chosen and divided into groups. MVPS 1 treated at the doses of 200， 400， 800 mg/kg and 1 200 mg/kg once daily， respectively. At 7th and 14th days， respectively， the levels of blood glucose， GSP， TCH and TG were measured. Other diabetic mice were divided into MVPS 1 (800 mg/kg)， MVPS 2 (180， 360 mg/kg) and MVPS 3 (2 040 mg/kg) groups. After 7 d treatment， both body weights and fasting blood glucose levels were measured. RESULTS MVPS 1 of 800 mg/kg displayed the optimal anti-hyperglycemic effect on diabetic mice and reduced GSP， TCH and TG levels of diabetic mice; MVPS 2 showed significantly anti-hyperglycemic activities， compared to the model group. MVPS 3 exhibited no anti-hyperglycemic activities. CONCLUSION MVPS 1 displays the optimal hypoglycemic effect at the dose of 800 mg/kg， and its hypoglycemic active site focuses on MVPS 2.

Investigation of TCM Syndromes on Maternal Separation in C57/BL6 Mice

BIANYao-yao, YANGLi-li, WANGZong-li, MEIZhen, WANGBei-lei, XUGui-hua

2015, 31(2): 138-142.

[Abstract](1001) [PDF 557KB](1142)

Abstract:
OBJECTIVE To investigate TCM syndromes on maternal separation in C57/BL6 mice. METHODS Neonatal mice were intervened through maternal separation. The effects of maternal separation on spontaneous activities of mice were evaluated by field test and the effects on immobility times of mice were evaluated by forced swimming test and tail suspension test. Four diagnostic quantitative indicators measurement and quantitative dialectical method were employed to explore the state of Qi， Blood， Yin and Yang in mice. Hypothalamus-pituitary-adrenal (HPA) cortical axis function was observed through adrenocorticotropic hormone (ACTH) and corticosterone (CORT) determination. The level of energy metabolism was investigated through the measurement of cyclic adenosine monophosphate/cyclic guanosine monophosphate (cAMP/cGMP)， ATPase and superoxide dismutase(SOD). RESULTS No statistical significance (P>0.05) was observed in the total path of animal activities between each group in field test. Compared with the control group and MS15 group， in the forced swimming test and tail suspension test， the immobility time of mice of MS18 group was significantly increased (P<0.01). The levels of Yang heat， cAMP/cGMP， Na⁺-K⁺-ATPase and SOD significantly decreased (P<0.01)， while ACTH and CORT significantly increased (P<0.01) of MS18 group， compared to the control group and MS15 group. CONCLUSION Maternal separation shows significant effects on behavior in mice. The mice exhibit depression-like behavior， upset HPA axis balance and reduce body energy metabolism， which characterized as Yang deficiency symptoms in TCM.

Study the Effects of Qianyang Yuyin Granules on Renal Blood Flow and Blood Pressures in Anesthetized Normal Dogs

SONGHeng-wen, SHILe, FANGZhu-yuan, ZHUXuan-xuan, ZHUFang-fang, SHENYang, ZENGTai-lin, XULi

2015, 31(2): 143-146.

[Abstract](1145) [PDF 349KB](1135)

Abstract:
OBJECTIVE To study the effects of Qianyang Yuyin Granules (QYG) on blood pressure and renal blood flow in anesthetized normal dogs and the mechanism of hypertensive renal injury treatment. METHODS The renal blood flow， cardiac output (CO) and blood pressures were determined by PowerLab system at 15， 30， 60， 90， 120， 180 min after QYG (3.08， 6.15， 12.30 g/kg) administration， respectively. RESULTS Compared with the control group， QYG (12.30 g/kg) reduced systolic blood pressure (SBP) significantly (P<0.05) at 120 min and 180 min， and increased the renal blood flow (P<0.05~0.01) at 90， 180 min; QYG (6.15 g/kg) reduced SBP (P<0.05) at 120 min， and increased the renal blood flow (P<0.05) at 90， 120 min. The effects of QYG (3.08 g/kg) were not obvious; The three QYG groups hardly affected CO (P>0.05). CONCLUSION QYG can increase the renal blood flow， improve the renal function and then reduce blood pressure.

Effects of Flos Puerariae-Semen Hoveniae Compatibility on Liver Function and Pathologic Morphology in Rats with Alcoholic Liver Disease

LIU Ming, CHEN Shao-hong, ZHONG Gan-sheng, LIU Hai-yan, ZHAO Tong

2015, 31(2): 147-151.

[Abstract](1190) [PDF 616KB](1154)

Abstract:
OBJECTIVE To explore the de-alcoholic and hepatoprotective function of Flos puerariae-Semen hoveniae (2∶1) compatibility and determine the optimal dosage regimen. METHODS Chronic alcoholic liver injury animal models were established by gavages of 56° Red Star Erguotou in rats. Experimental animals were given different doses (3， 6， 12 g/kg) of Flos puerariae-Semen hoveniae (2∶1) extracts within different medication courses (4， 8， 12 weeks). The liver index， ALT， AST， TP， ALB and ALP in serum were detected. The liver pathological morphologies were also observed. RESULTS Chronic alcoholic liver injury models were successfully replicated. Flos puerariae-Semen hoveniae (2∶1) compatibility group of each dose displayed no therapeutic effect within four weeks. The level of ALT in Flos puerariae-Semen hoveniae (2∶1) compatibility group of each dose significantly decreased (P<0.05~0.01) after eight weeks administration. After 12 weeks， Flos puerariae-Semen hoveniae (2∶1) compatibility groups showed significantly decreased AST and increased ALB (P<0.05). Low and high dose groups displayed reduced ALT (P<0.05) while the low dose group showed increased TP (P<0.01). The changes in liver pathological morphology were fairly consistent with relevant indicators of liver function. CONCLUSION Flos puerariae-Semen hoveniae (2∶1) compatibility group of each dose shows promising de-alcoholic and hepatoprotective effects after eight weeks of administration. The relevant indicators of liver function improve to varying degrees as the therapy time prolongs. Among them， low dose group performs better than medium and high dose groups.

Antipyretic Effects and Immune Regulation Function of Tongfu Xiere Therapy in Fever Rats with Meningitis

LIU Ya-juan, ZHANG Si-chao, WANG Xiao-jun, QIN Xiao-yun

2015, 31(2): 152-155.

[Abstract](977) [PDF 428KB](1114)

Abstract:
OBJECTIVE Based on the brain-gut axis theory， to investigate the antipyretic effect and immune regulation function of Tongfu Xiere therapy in fever rats with meningitis. METHODS The rat fever model induced bacterial Meninggitis was established with injecting the Escherichia coli into cerebellomedullary cistern. The experimental animals were divided into six groups: the normal group， the model group， Dachengqi decoction group， Xingnaojing injection group， Dachengqi decoction+Xingnaojing injection group and paracetamol group. Antipyretic effect of each drug was observed by measuring body temperature after treatment. The levels of IFN-γ， IL-4 and SP in plasma were detected by enzyme-linked immunosorbent assay. RESULTS Compared to the model group， Dachengqi decoction group， Xingnaojing injection group and Dachengqi decoction+Xingnaojing injection group significantly reduced the temperature of the fever rats（P<0.05）， the levels of IFN-γ（P<0.01）， IL-4（P<0.05）and SP（P<0.05）， and the ratio of IFN-γ/IL-4（P<0.05）. CONCLUSION Tongfu Xiere therapy shows antipyretic effect on fever rats with Meningitis， which is related to immune regulation biologically mediated by brain-gut axis components.

The Neuroprotective Effects of Yueju Pills on Parkinson's Disease Model in vitro

CHENChang, TANGJuan-juan, XIABao-mei, CHENQing, WANGQi-sheng, TAOWei-wei, ZHAOYang, CHENGang

2015, 31(2): 156-159.

[Abstract](936) [PDF 570KB](1078)

Abstract:
OBJECTIVE To investigate the neuroprotective effect and the mechanism of Yueju alcohol extract (YJ-E) on chromaffin cells (PC12) injury in rats induced by 1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine ion (MPP⁺). METHODS PC12 cells were given 1 mmol MPP⁺ to establish Parkinson's disease (PD) model in vitro. The protective effects of YJ-E on PC12 cells were measured by MTT assay and Hoechst staining. The expression of pituitary adenylate cyclase activating polypeptide (PACAP) and the phosphorylation of extracellular regulated kinase (ERK) and cAMP response element binding protein (CREB) were determined by Western blotting. RESULTS YJ-E (1.0， 2.0， 4.0 mg/mL) improved the survival of MPP⁺-injured PC12 cells significantly (P<0.01)， and displayed no significant effect on normal PC12 cells (P>0.05). Meanwhile， YJ-E up-regulated the expression of PACAP and the phosphorylation of ERK and CREB significantly (P<0.01) of MPP⁺-injured PC12 cells. CONCLUSION YJ-E shows promising neuroprotective effects on PC12 cells in MPP⁺-induced Parkinson's disease model， and the mechanism may be associated with the up-regulation of the expression of PACAP and the phosphorylation of downstream ERK and CREB.

Immune Cell Proliferation Function and in vitro Antioxidant Activity of n-butanol Extract of Radix Isatidis

YANG Jing-yan, HE Li-wei, DONG Wei

2015, 31(2): 160-164.

[Abstract](1103) [PDF 428KB](1058)

Abstract:
OBJECTIVE To investigate proliferative activities for peritoneal macrophages and splenic lymphocytes in mice and anti-oxidative potency in vitro of n-butanol extract of Radix isatidis. METHODS 12 components were extracted and separated from Radix isatidis by n-butanol. Each component of total n-butanol extracts was detected and analyzed by HPLC. The proliferative activities of each component for peritoneal macrophages and splenic lymphocytes were detected by MTT assay. Anti-oxidative activities were evaluated by DPPH and ABTS radical scavenging and FRAP method. RESULTS No. 2， 6， 7 and 11 components showed the most potent proliferation on immune cells， with the highest proliferation index>3. No. 2 and 6 components displayed the strongest antioxidant activities， with IC₅₀ values on DPPH of 0.391， 0.164 mg/mL， ABTS of 0.164， 0.338 mg/mL and Fe³⁺ reducibility values of 1.441， 0.812 mmol/g equivalent weight of FeSO₄， respectively. CONCLUSION The n-butanol extract of Radix isatidis exhibit promoting proliferation for peritoneal macrophages and splenic lymphocytes and pontent antioxidant activities. Further investigation of the chemical structure will help to clarify the material basis for Radix isatidis antiviral effects.

Protective Effects of Total Flavonoids from Bamboo Leaves on UVB-irradiated Oxidative Damage in HaCaT Cells

GAOYang, YIRuo-Kun, SONGJia-Le

2015, 31(2): 165-169.

[Abstract](966) [PDF 470KB](1420)

Abstract:
OBJECTIVE To investigate the protective effects of total flavonoids from bamboo (Phyllostachys pubescens) leaves (BLE) on UVB- irradiated oxidative damage in HaCaT keratinocytes. METHODS The HaCaT cells were pretreated with BLE for 24 h and then exposed to UVB (20 mJ/cm²) for 2 h. Cell viability was determined by MTT assay. The levels of reactive oxygen species (ROS)， lipid peroxidation and antioxidant enzymes including catalase (CAT)， superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. mRNA transcription levels of these antioxidant enzymes were determined by RT-PCR assay. In addition， the production of TNF-α and IL-6 were detected by ELISA assay. RESULTS BLE significantly reduced the UVB-induced HaCaT cell damage and improved the cell survival rates (P<0.05). Compared to the control group， BLE decreased the levels of ROS and lipid peroxidation， and increased the activities and mRNA transcription levels of endogenous antioxidant enzymes (SOD， CAT and GSH-Px) in UVB-irradiated HaCaT cells. In addition， BLE also inhibited the secretion of TNF-α and IL-6 in UVB-irradiated HaCaT cells. CONCLUSION BLE can exhibit cytoprotective activities against UVB-induced oxidative damage in HaCaT cells through the reduction of ROS， inhibition of lipid peroxidation and regulation of antioxidant enzymes activities. In addition， BLE can also decrease the levels of TNF-α and IL-6 in UVB-irradiated HaCaT cells.

Effects of Radix Saposhnikoviae on Metabolism of Calycosin-7-O-β-D-glucoside in Radix Astragali by Intestinal Flora Experiment in vitro

LIULing, ZHAOXiao-li, DILiu-qing, LIJun-song

2015, 31(2): 170-173.

[Abstract](1020) [PDF 549KB](1111)

Abstract:
OBJECTIVE To study the effects of Radix Saposhnikoviae on the metabolism of calycosin-7-O-β-D-glucoside as the main active component in Radix Astragali in intestinal flora of rats， exploring the scientific connotation of compatible application of Radix Astragali-Radix Saposhnikovia from the perspective of metabolism. METHODS The concentrations of calycosin-7-O-β-D-glucoside and calycosin between Radix Astragali group and Radix Astragali-Radix Saposhnikoviae group， as well as calycosin-7-O-β-D-glucoside monomer group and calycosin-7-O-β-D-glucoside-Radix Saposhnikoviae group at different time points were compared， respectively， by intestinal flora experiments. RESULTS Compared to Radix Astragali group， the concentration of calycosin-7-O-β-D-glucoside significantly increased in Radix Astragali-Radix Saposhnikoviae group (P<0.01)， while the concentration of calycosin significantly decreased (P<0.01) at different time points. Compared to monomer group， the concentration of calycosin-7-O-β-D-glucoside was significantly higher than that in calycosin-7-O-β-D-glucoside-Radix Saposhnikoviae group (P<0.01) and the concentration of calycosin was significantly lower (P<0.01). CONCLUSION Radix Saposhnikoviae can inhibit the transformation from calycosin-7-O-β-D-glucoside to calycosin and slow its metabolism.

The Choice of Preferred Materials of Ginkgolide Solid Dispersions

MAYing, LYUZhi-yang, DILiu-qing, LIJun-song, KANGAn

2015, 31(2): 174-178.

[Abstract](1068) [PDF 1107KB](1263)

Abstract:
OBJECTIVE Choosing the optimizing assistance materials and the best proportional to make the solid dispersion of Ginkgolides， which so that can tremendously improve the dissolution rate of Ginkgolides. METHODS We determine the dissolution rate of the solid dispersion respectively， which are made by Eudragit EPO and PVP K30 in different drug-material ratio. In addition， quantitative phase determination of them are measured by DSC， X-RD and SEM. RESULTS The dissolution rate of the solid dispersion with the Ginkgolides -EPO ratio in 1∶2， 1∶4， 1∶6， 1∶8 were 65%， 68%， 70% and 71% respectively in five minutes. In the corresponding proportion， The dissolution rate of the solid dispersion made by PVP K30 were 40%， 50%， 66%， 70% respectively. In the same time， the dissolution rate of the drug substance were 10%. Phase identification results showed that Ginkgolides can evenly dispers in amorphous state in the solid dispersion. CONCLUSION The solid dispersion with the Ginkgolides -EPO ratio of 1∶2 is the best choice to preparation， which has the tremendous drug-loading and dissolution efficiency， thereby having a excellent prospect of application.

Study on Enriching Total Alkaloids and Total Phenols from Mulberry by Cation Exchange Resin and Macroporous Absorption Resin

CHAIYao, CHENGJian-ming, PENGJiu-man, LUjie-lin, DONGWen-nan, LIURui, WUHao

2015, 31(2): 179-182.

[Abstract](1115) [PDF 569KB](1095)

Abstract:
OBJECTIVE To enrich the total alkaloids and total phenols from mulberry. METHODS Combined appling cation exchange resin and macroporous absorption resin to enrich and purify the total alkaloids and total phenols， then verify and optimize the process. RESULTS The process contributed to enrich and purify the total alkaloids and total phenols from mulberry. The content of DNJ， chlorogenic acid and rutin can achieve 0.17%， 3.05% and 0.92%. CONCLUSION Using cation exchange resin and macroporous absorption resin to concentration of the total alkaloids and total phenols may be applied for the real bulk production.

Efficacy Evaluation of Moxibustion Therapy for Angina Patients with Coronary Artery Disease

ZHANGZe, CHENMin, WUWen-sheng, ZHANGLi, HUAZhen, YANGGuan-lin

2015, 31(2): 183-186.

[Abstract](1100) [PDF 732KB](1126)

Abstract:
OBJECTIVE To evaluate the benefits and side effects of moxibustion therapy for coronary artery disease. METHODS Relevant studies of moxibustion therapy for angina were collected from databases including Pubmed， Embase，Cochrane library， CNKI， Wanfang Database and CBMDisk. The inclusion and exclusion criteria was applied when the literature was screened， data was extracted and the quality of the included studies were assessed independently. Then， meta-analysis was performed by using RevMan 5.2 software. RESULTS 1 318 patients and 13 researches about moxibustion therapy for coronary artery disease were collected. The Meta analysis showed that compared with the control group， the western medicine group[RR=1.22，95%CI(1.11，1.33)]and the acupuncture group[RR=1.5，95%CI(1.23，1.83)]， the moxibustion group showed great improvement in relieving angina pectorisis and good effects were shown in the ECG[RR=1.23，95%CI(1.14，1.33)]. In terms of blood liquids， the moxibustion group showed good effects in regulating triglyceride[SMD=-0.81，95%CI(-1.57，-0.06)]， low density lipoprotein cholesterin[SMD =-0.55，95%CI(-1.04，-0.06)]， and high density lipoprotein cholesterol[SMD=0.43，95%CI(0.2，0.65)] and the changes were of statistical significance. Compared with the Chinese medicine group， the improvement in angina pectorisis of the moxibustion group showed no statistical significance[RR=1.15，95%CI(0.99，1.35)]. The difference between the changes of total cholesterol in the treatment group and the control group had no statistical significance[SMD=-0.74，95%CI(-1.75，0.27)]. CONCLUSION Moxibustion is a unique TCM therapy in treating angina pectorisis and can be applied in clinical practices. Meanwhile， the outcome of this system was not perfect. And more high quality of randomized controlled trials are needed to verify the effect of the therapy.

Comprehensive Interventional Therapy with the Combination of Chinese and Western Medicine for Community Senior Patients with Diabetes: A Study of 44 Cases

GUQin, TENGShi-chao, CHENGYan, WANGPei, GAOXiang-li, WANGLing-cong, MAXiao-ying

2015, 31(2): 187-189.

[Abstract](945) [PDF 354KB](1123)

Abstract:
OBJECTIVE To observe the clinical effects of integration of traditional Chinese medicine and western medicine in treating senile diabetes and explore proper community management for diabetes in our country and improve the therapeutic effect of diabetes treatment. METHODS All cases were randomly divided into 2 groups (control group and experiment group) after intervention. The control group (46 cases) were treated by comprehensive western treatment， while experiment group (44 cases) were given both comprehensive western treatment and Chinese patent. Changes of blood pressure， blood glucose， glycocylated hemoglobin and life quality score were observed in two groups. RESULTS After intervention， levels of fasting blood glucose in two groups both decreased compared to that of pre-intervention (P<0.05). Decreased levels of fasting blood glucose showed statistical difference between them (P<0.01). There was no difference in levels of postprandial blood glucose before and after treatment (P>0.05); both levels of glycocylated hemoglobin in two groups decreased (P<0.05)， and the experiment group were much more obvious than that of control group (P<0.05). There was no statistical significance between two groups in differences of systolic and diastolic pressures before and after treatment (P>0.05). When it comes to life quality， vitality (VT)， mental health (MH) scores， the experiment group got improvement (P<0.05) and the psychological evaluation score was higher after treatment (P<0.05). In control group， general health (GH)， vitality (VT)， mental health (MH)， and psychological evaluation got improvement after treatment， which is significant statistically (P<0.05). While the difference between two groups didn't show any statistical significance (P>0.05). CONCLUSION Comprehensive intervention is successful in dealing with chronic diseases like diabetes and integration of traditional Chinese medicine and western medicine is effective in treating senile diabetes.

Clinical study on Qici Method in Treating Simple Obesity together with Heat and Dampness in the Stomach

WEIWei, CHENQuan-li, WANGYuan-jun, LIJuan

2015, 31(2): 190-193.

[Abstract](1023) [PDF 379KB](1053)

Abstract:
OBJECTIVE To compare the clinical effects of needling in the abdominal acupoints and diet only in treating simple obese patients with heat and dampness in the stomach. METHODS random number table method was adopted to divide 86 obese patients with heat and dampness in the stomach into two groups. There were 44 patients in the treatment group with 3 being uncounted later and the treating method was needling in the abdominal acupoints and certain acupoints in the body. There were 42 patients in the control group with 2 being uncounted later and they were treated with diet only. The certain indexes related to obesity， lipid levels， and nuclear magnetic resonance scanning results were recorded and compared before and after the treatment. CONCLUSIONS The certain indexes related to obesity， lipid levels， and nuclear magnetic resonance scanning results of both groups were lower compared with before(P＜0.05~0.01).And the results of the treatment group were lower than the control group(P＜0.05). The total effective rate of the treatment group after two periods of treating was higher than that of the control group(P＜0.05). CONCLUSIONS The clinical effect of needling in the abdominal acupoints is better than diet only in loosing weight and is worth of being widely used.

Research Advances in the Correlation between Precancerous Lesion of Chronic Atrophic Gastritis Genes and TCM Syndrome Type

SHIJia-ning, HAOWei-wei

2015, 31(2): 194-196.

[Abstract](948) [PDF 358KB](1056)

Abstract:

Animal Model of Bone Cancer Pain and Its Application in the Study of Traditional Chinese Medicine

WANG Chang-ming, YU Guang, MENG Yi-qian, TANG Zong-xiang

2015, 31(2): 197-200.

[Abstract](1013) [PDF 387KB](1244)

Abstract:

2015 Vol. 31, No. 2

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