2014 Vol. 30, No. 5

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On Cancerous Toxin and Treating Methods
ZHANGYong-hong, ZHANGXun, YELi-hong, PENGHai-yan
2014, 30(5): 401-403.
[Abstract](1042) [PDF 1045KB](1274)
Abstract:
The authors consider cancerous toxin can be sumarized for cancer-causing toxic factors that cause canceration of zang-fu organs and cancer cells. Cancerous toxin may be caused by several reasons: the external pathogens and internal pathogen; and cancer cells in the body organ can also be the source of cancerous toxin. Cancerous toxin has carcinogenicity， latency， transitivity， and it can cause malignant growths， high consumption and is hard to be treated. The treating method for the cancer can be applied to patients with solid tumor， metastasis or recurrence or transfer tendency. Treating methods include combating poison with poison， detoxification， removing blood stasis to treat toxin， resolving phlegm to treat toxin， and strengthening healthy energy to remove toxin.
On Stomach Wind
YE Bo, CHEN Jing
2014, 30(5): 404-405.
[Abstract](5432) [PDF 1027KB](3393)
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Discussions on stomach wind by doctors in different periods have been summarized in this paper. The authors think that the clinical manifestations of stomach wind are shown in gastrointestinal tract and nervous system， which is similar to the theory of brain-gut axis in modern medicine. Since the treating principals， methods and formulas are quite useful for the clinical symptoms， it is worthwhile to study on them.
Treatment of Diabetic Vascular Complications in Luobing Theory
SONG Ying-ying, YANG Jin
2014, 30(5): 406-409.
[Abstract](892) [PDF 1068KB](1618)
Abstract:
Diabetic vascular complications are specific damages caused by diabetes mellitus belonging to Vessel and Collateral Damage of Xiaoke Disease. Large vessel diseases and small vessel diseases related to diabetic complications can be categorized to Luobing. As collateral damage is considered as the pathogenesis of diabetic vascular complications according to Luobing theory， expelling blood stasis and dredging collateral should be applied to inhibit the early middle and late stages of diabetes mellitus. According to the extent of collateral obstruction， there are three stages: collateral qi stagnation， collateral blood stasis and collateral blockage and expelling blood stasis and dredging collateral medicines with different effects should be applied in different stages in order to improve clinic effects.
On Crohn's Disease from TCM Perspective
ZHAO Yan-hua, ZHAO Zhi-qiang
2014, 30(5): 410-412.
[Abstract](2229) [PDF 1042KB](3146)
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Crohn's disease manifests complicated symptoms and it has different names in TCM. The pathogenesis is related to diet， congenital conditions， exogenous evils， smoke， drug and other diseases. As for the etiology， they are evils hurting the body， stagnation resulting in heat and evils in the body， phlegm and blood stasis， spleen and stomach damage and the deficiency of vital qi， etc. Main treating methods include clearing away heat and cool the blood， resolving blood stasis and detoxification， drying the dampness and resolving the phlegm， softening hard lumps and dispelling nodes and strengthening the spleen and vital qi.
Clinical Decisions for Acupuncture Treatment in Sudden Hearing Loss Based on Clinical Evidences and Experts Opinions
LI Bo, DU Yuan-hao
2014, 30(5): 413-416.
[Abstract](941) [PDF 1071KB](1169)
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The Principles of Acupoint Selection in Treatment of Coronary Artery Disease Based on Data Mining
CHENXia, LUSheng-feng, ZHUBing-mei, NIGuang-xia
2014, 30(5): 417-421.
[Abstract](4973) [PDF 1136KB](2956)
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OBJECTIVE To excavate the principles of acupoint selection for coronary artery disease (CAD) by using statistics and data mining methods. METHODS The literatures meeting the inclusive criteria on CAD were collected from three major Chinese database and PubMed database from 1991 to 2013. With these literatures， we collected data of acupoints for CAD treatment and then did descriptive analysis， association analysis and clustering analysis to the data. RESULTS There was no significant difference in the number of papers published in 1991-2013. Neiguan (PC6)， Xinshu (BL15)， and Danzhong (RN17) were listed as the top three acupionts with the highest frequency of application. The correlation analysis showed that Neiguan (PC6) and Xinshu (BL15) had the highest correlativity. The clustering analysis figured out the key acupoints group and optional acupoints group. CONCLUSION The study discussed the principles of acupoint selection in the treatment of CAD， at least partly suggesting the principles for clinical application of acupuncture and moxibustion treatment on coronary artery diseases.
Clinical Characteristics of HCV Genotype of Refractory Hepatitis C and its Effect on Antiviral Function of Qizhu Granule
ZHAO Lin, CHEN Jian-jie, Nie Hong-ming, LING Qi-hua, CHEN Yi-yun, YE Qing-yan, SHEN Hong
2014, 30(5): 422-424.
[Abstract](933) [PDF 1056KB](1018)
Abstract:
OBJECTIVE To investigate clinical characteristics of HCV genotypes of refractory hepatitis C and its effect on antiviral function of Qizhu Granule. METHODS After virus genotypes were analyzed by SANGER sequencing before the treatment， 204 patients with refractory hepatitis C were randomly and equally divided into the treatment group (Qizhu Granules) and the control group (placebo group). Two groups received 48-week treatments. The patients' HCV RNA level was monitored before treatment and during the 4th， 12th， 24th， 48th treatment， as well as 4 weeks after the treatment. RESULTS 182 patients finally received 48-week treatment and completed the study.173 cases were detected with their genotypes， including 129 case of genotype 1b for 74.6%， and 44 cases of non-1b type for 26.4%. No significant difference was found between genotype 1b and non-genotype 1b patients in gender， BMI index and serum ALT and AST level (P>0.05). Patients infected by genotype 1b virus had longer disease courses and were older than non-genotype 1b patients on average. RNA level of HCV of genotype 1b patients was higher than that of non-1b type with significant differences. SVR in the treatment group of non-genotype 1b patients was 42.86%， while that of genotype 1b was only 9.09% with statistically significance. CONCLUSION Non-1b genotype patients have better antiviral effects and HCV genotype is an important factor affecting the antiviral effect of Qizhu granules.
Nasal Endoscope Postoperative Clinical Research on Chronic Rhinosinusitis Treated by Nasal Irrigation with QingDouLing Mixture
LILiang-bo, GONGCheng, LIAOYong, YUANWei, CHENSheng-jing, WANGZhen-rui
2014, 30(5): 425-428.
[Abstract](973) [PDF 1080KB](1257)
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OBJECTIVE To observe the clinical efficacy on the chronic rhinosinusitis patients treated by nasal irrigation with QingDouLing Mixture. METHODS 60 cases of chronic rhinosinusitis and nasal endoscopic surgery were randomly divided into treatment group and control group with 30 cases in each group. The control group had nasal irrigation by using 0.9% sodium chloride injection， treatment group had nasal irrigation by using 0.9% sodium chloride injection with QingDouLing Mixture added. Nasal mucociliary transport rates (MTR) in both groups were measured before and 1 month， 3 months and 6 months after operation respectively. Follow-up visiting was kept for over 6 months to observe the clinical curative effect of the two groups. RESULTS MTR in treatment group of postoperation for 1 month and 3 months was faster than that of the control group. There was significant difference between two groups (P<0.05). There was no significant difference on MTR between the two groups when 6 months (P>0.05). Compared 3 months with 6 months， after the operation the overall control rate and total effective rate in treatment group were significantly higher than that of the control group， with significant difference (P<0.05). CONCLUSION QingDouLing Mixture for nasal irrigation has good curative effect on many aspects， such as controlling the inflammation of the nasal cavity， reducing secretion， improving clinical symptoms， reducing the operation cavity edema to prevent granulation and vesicle formation， promoting the operation cavity epithelization， and promoting early recovery of nasal mucociliary function， etc.
Clinical Observation on Shenque Point Drug Administration for Shenque Acupoint in Treating Diarrhea-Predominant Irritable Bowel Syndrome
ZHANGJi-wei, SUNJian-hua, ZHANDao-wei, LUWei-min, WUXiao-liang, ZHANGWei, JIAODai-yan
2014, 30(5): 429-431.
[Abstract](936) [PDF 1051KB](977)
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OBJECTIVE To evaluate the differences between point drug delivery's clinical effect on diarrhea-predominant irritable bowel syndrome due to yang insufficiency of both spleen and kidney and the western medicine effect. METHODS 58 patients were randomly divided into two groups by using simple random number table method. 29 cases in treatment group were undertaken drug delivery on Shenque point， of which there were 3 cases defluxion. 29 cases in control group took Decetel and Jinshuangqi of which there were 2 cases defluxion. Clinical curative effect comparison was done after treated for 4 weeks for these two groups and the effect on IBS-D patients belonging to yang insufficiency of both spleen and kidney was evaluated. RESULTS The total effective rate of treatment group was 88.46%， superior to control group of 62.96% (P<0.05). There was statistic significance between two groups (P<0.05). CONCLUSION Point drug delivery with the advantage of improving clinical symptoms is effective on IBS-D patients due to yang insufficiency of both spleen and kidney syndrome.
Clinical Observation on Danggui Sini Decoction in Preventing and Treating Oxaliplatin Neurotoxicity
DINGRong, HUOJie-ge, WANGYue
2014, 30(5): 432-433.
[Abstract](924) [PDF 1033KB](1470)
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OBJECTIVE To observe the efficacy of Danggui Sini Decoction in preventing and treating oxaliplatin neurotoxicity. METHODS 48 patients in accordance with criteria were randomly divided into treatment group and control group with 24 cases in each. All the patients had been treated with chemotherapy which contained oxaliplatin. Furthermore， treatment group were simultaneously given Danggui Sini Decoction. Each group was treated for four courses with 21 days in each course. The neurotoxicity classification and clinical therapeutic effect of Chinese Medicine were observed after treatment. RESULTS The total effective rate of TCM syndromes after therapy was 70.8% and 37.5% respectively in treatment group and the control group (P<0.05). The total incidence of neurotoxicity after therapy of the treatment group and the control group(P<0.05). CONCLUSION Danggui Sini Decoction is effective on preventing and treating oxaliplatin neurotoxicity.
The Recent Observation of Therapeutic Effect on Comprehensive Intervention with Traditional Chinese Medicine for Degenerative Lumbar Instability Syndrome in the Community
HUAZhen, WANGJian-wei, ZHANGYa-feng, YANGJun-feng, LUZhen-fei, WANGShan-fu, PANYa-lan
2014, 30(5): 434-437.
[Abstract](1417) [PDF 1075KB](1445)
Abstract:
OBJECTIVE To explore the effect on comprehensive intervention with traditional Chinese medicine in the community for degenerative lumbar instability syndrome so as to provide supports and aids for establishing a comprehensive intervention protocol suitable for China and on the basis of community. METHODS All the patients of degenerative lumbar instability syndrome meeting the criteria were divided into the intervention group with 28 cases in it and the control group with 36 cases in it. Intervention group were given symptomatic treatment， accompanied with comprehensive intervention including health lecture， psychological counseling， functional exercises and various conservative treatment such as fumigation with herbs， acupuncture and moxibustion， acupotomy， maneuver， while control group were only provided symptomatic treatment. Both of the two groups were followed up for 6 months to evaluate following aspects: awareness of the disease， changes of clinical symptoms， the surgery rate and the medical imaging. RESULTS The intervention group's score of awareness of the disease was higher than that of control group with statistical significance (P<0.01). There was no statistical significance of JOA score and Oswestry Disability Index (ODI) in both groups before treatment (P>0.05). Improvement of JOA score and ODI can be observed in two groups after treatment. There was a statistical significant (P<0.01). Compared with control group， post-treatment JOA score of intervention group was higher (P<0.05)， as well as good rate of JOA (P<0.01). However， ODI and ratio of surgery of intervention group were lower than that of control group (P<0.05). There was no statistical significance of the changes of medical imaging during the 6 months (P>0.05). CONCLUSION Comprehensive intervention with traditional Chinese medicine has an obvious effect on treating degenerative lumbar instability syndrome which can alleviate the clinical symptoms， enhance awareness of the disease， decrease the surgery rate and improve the life quality of patients.
Study on the correlation between the salt-roast processing and the anti-osteoporosis efficacy of Qing'e Pill
YANCui-ping, WENGZe-bin, WUYu, CHENZhi-peng, CAIBao-chang, LIWei-dong
2014, 30(5): 438-442.
[Abstract](1010) [PDF 1829KB](1040)
Abstract:
OBJECTIVE To study the salt-roast processing on Qing'e pill warming kidney and strong bones efficacy. The traditional Chinese medicine theory revealed that salt-roast medicines can lead to drug to kidney meridian， enhance the effect of warming kidney and activate yang. This research studied the different methods processed Qing’e Pill on ovariectomy (OVX) induced osteoporosis in rats， and to explore the mechanism of the antiosteoporotic effects of Qing’e Pill on osteoporosis． METHODS After OVX rats oral administrating of the Qing’e Pill with the combination of crude and salt-roast medicines at the dose of 0.9g/day for 12 weeks， the BMD of rat left femur metaphysis was determined by using dual-energy X-ray absorptiometry and the bone turnover makers in serum were measured to evaluate anti-osteoporosis effect of different processed Qing'e pill. In addtion， the trabecular microstructure of femoral metaphyseal was analyzed by Micro-CT scanning and HE staining. RESULTS Treating with Qing'e pill suppressed weight growth of ovariectomized induced rats fat gain， increased uterine weight， and no significant difference was observed between crude and salt-roast Qing'e pill treatment groups. It also increased the rat femur BMD， and improved the density of trabecular bone and protected the trabecular bone from damage caused by ovariectomy. And all results showed that the treatment of salt-roast processing Qing'e pill is better than crude Qing'e pill. Meanwhile， the crude and salt-roast Qing'e pill treatment did not cause lesions in the rat uterus. CONCLUSION Qing'e pill may have a good therapeutic effect for the treatment of osteoporosis rats induced by ovariectomy and after salt-roast processing the anti-osteoporosis efficacy of Qing'e pill was significantly enhanced. Both crude and salt-roast Qing'e pill treatment will not cause uterine lesions within suitable therapeutic dose range.
Effect of Electroacupuncture on Interleukin-6 Receptor mRNA Expression in Hippocampus of Cerebral Ischemia-reperfusion Rats
MUYan-yun, CHENGJie, XIAYou-bing, SHENMei-hong, SHENJie, CHENXia, LIQian, SUNYong, GONGMei-rong
2014, 30(5): 443-446.
[Abstract](927) [PDF 1151KB](994)
Abstract:
OBJECTIVE To observe the changes of IL-6 receptor in hippocampus after cerebral ischemia-reperfusion and the effect on IL-6 receptor expression by electroacupuncture treatment. METHODS Sprague Dawley rats were randomly divided into sham-operation (sham)， model and EA groups (five rats in each group). There are two observing time in each group: 6 hours and 24 hours after reperfusion. CI/R model was established by right middle cerebral artery occlusion for 2 h and reperfusion for 24 h. EA (3Hz， 1-3mA) was applied to "Baihui" (DU20)， "Dazhui" (DU14) for 30 min. the expression of interleukin-6 receptor mRNA and gp130 mRNA in hippocampus by q-PCR method was observed. RESULTS ①The expression of interleukin-6Rα mRNA decreased as time changed: At the sixth hour， the expression of interleukin-6 Rα mRNA in the model group was the least compared with the sham group and EA group. There was significantly difference (P<0.01) compared with the sham group and (P<0.05) compared with the EA group; At the twenty-forth hour， the expression of interleukin-6 Rα mRNA of every group decreased， the expression of interleukin-6 Rα mRNA in the model group was still the least compared with the sham group (P<0.01)， there was no significant difference between model group and EA group. ②The expression of gp130 mRNA increased as time changed; at the sixth hour， there was no difference in the three groups. At the twenty-forth hour， and the expression of gp130 mRNA in the sham group had no significant changes， the expression of gp130 mRNA of the model group and the EA group rose， the expression in the model group was less than that of the EA group significantly (P<0.05); while compared with the sham group， the expression of gp130 mRNA rose significantly (P<0.01) in EA group. CONCLUSION EA can up-regulate the expression of interleukin-6Ra mRNA and gp130 mRNA in rats' hippocampus of cerebral ischemia-reperfusion models. This may be one of the mechanisms of EA in protecting brain injury.
Effects of Acupuncture Combined with Chinese Medicine on Tear Secretion and Acetylcholine Content in Dry Eye Rabbit Model
LIU Xue, XU Wen, GAO Wei-ping
2014, 30(5): 447-449.
[Abstract](920) [PDF 1117KB](1123)
Abstract:
OBJECTIVE To explore the curative mechanism of acupuncture and Chinese medicine for dry eye syndrome by observing the changes of tear secretion and tear ducts acetylcholine content in the rabbit model. METHODS New Zealand white rabbits were randomly divided into the blank group， model group， acupuncture group， Chinese medicine and acupuncture and Chinese medicine group (10 rats in each group). Patients in the blank group did not receive any treatment. Model group was treated with eyes drops of 1% atropine sulfate eye drops (four times a day until the end of the trial). The remaining group was treated with acupuncture， Chinese medicine and combination of acupuncture and Chinese medicine respectively. The lacrimal tear secretion and acetylcholine in each group at different stages were observed. RESULTS Acupuncture， Chinese medicine and acupuncture combined with Chinese medicine can improve tear secretion in dry eye rabbit model. In the 10th and 17th day of the experiment， the lachrymal acetylcholine levels in acupuncture combined with Chinese traditional medicine group were significantly higher than those of the model group (P<0.05)， and there was significant difference. CONCLUSION Acupuncture combined with Chinese medicine runmuling can obviously promote the Ach secretion of lacrimal gland and increase tear secretion.
Effects of Gua Sha therapy on IgG in Serum and Pathological Changes of Disc Tissue of Rats with Lumbar Disc Herniation
CHENHua, XUGui-hua, JIANGRong-rong, LIXiao-chun, GuoYang
2014, 30(5): 450-453.
[Abstract](954) [PDF 1192KB](1015)
Abstract:
OBJECTIVE To explore the mechanism of Gua Sha therapy on the model rats with lumbar disc herniation (LDH). METHODS Fifty male SD rats were divided into 5 groups randomly: blank group， sham operation group， model group， Gua Sha group and drug group (n=10 each group). The rats of blank group did not receive any treatment， of sham operation group with sham operation， of model group， Gua Sha group and drug group were modeled:transplant nucleus pulposus of rat's coccygeal vertebra disc to the left muscle layer near L4~L5 by operation， among which model group were given no intervention; Gua Sha group were given Gua Sha therapy on the 5th day after operation; Drug group were fed with azathioprine through intragastric gavage (3 mg/kg)， followed by drawing blood on the day before operation， the 4th day after operation (the day before treatment)， the 6th day after interventions， the 12th day after interventions and the 18th day after interventions (the end of treatment) and detecting the IgG in serum by ELISA method. After the last blood drawing， all rats were killed. The corresponding coccygeal vertebra disc of the rats in blank group and sham operation group and the transplanted coccygeal vertebra discs in model group， Gua Sha group， and drug group were taken out to observe pathological changes of coccygeal vertebra disc under optical microscope. RESULTS Compared with the model group， serum level of IgG of Gua Sha group and drug group were obviously decreased， with significant difference (P<0.01)， but without statistical significance between the two groups (P>0.05)， suggesting that both Gua Sha therapy and drug could inhibit the autoimmunity reaction caused by modeling， with similar effects on lowering serum level of IgG in model rats. The pathological slice of intervertebral tissue showed that there was no obvious inflammation reaction in sham operation group， and the topical immune-inflammatory reaction of intervertebral peripheral tissue in Gua Sha group and drug group was obviously alleviated. CONCLUSION Gua Sha therapy could inhibit the autoimmunity and immuno-inflammatory reaction caused by transplantation of nucleus pulposus.
Study on Experimental Anti-adhesion Effect of Huoxue Tongfu Fang and the Mechanism
SONGYu, ZHANGHui, WANGShuang, ZHANGChong, CUITai-zhen, NIUBing-xuan, HAIGuang-fan, ZHANGXiao-yi, ZHANGTing, ZENGLi
2014, 30(5): 454-457.
[Abstract](925) [PDF 1102KB](1173)
Abstract:
OBJECTIVE 60 male SD rats were randomly divided into six groups: normal control group， model group， low， medium and high dose of Huoxue Tongfu Fang groups and dexamethasone sodium phosphate positive control group. All groups were prepared for adhesion models except normal control group; control group and model group were injected with saline; dexamethasone group received intraperitoneal injection of dexamethasone sodium phosphate (10 mg/kg); low， medium and high doses of Huoxue Tongfu Fang groups orally administered Huoxue Tongfu Fang (0.65， 1.3， 2.6 g/100 g) a week for all. On the 8th day， blood in each group was taken out and IL-6， IL-1β and TNF-α in serum were determined in ELISA method; adhesion tissue was taken and TGF-β1 and CTGF levels were detected with ELISA kit after homogenate; adhesion degree was recorded. The expression of FN mRNA in adhesion tissue was tested in RT-PCR; p-Smad 3 protein level was detected in Western blot. RESULTS IL-1β and TNF-α in model group increased; so did the expression of FN mRNA， TGF-β1 and CTGF levels. Compared with model group， the degree of adhesion in all doses of Huoxue Tongfu Fang significantly reduced; IL-1β and TNF-α in serum and TGF-β1 and CTGF levels significantly declined (P<0.05~0.01); the expression of FN mRNA and p-Smad 3 decreased (P<0.05~0.01). CONCLUSION Huoxue Tongfu Fang can reduce the degree of adhesion， making a preventive and therapeutic effect on adhesion.
Preparation and Determination of Rabbit Knee Osteoarthritis Model
TANGXiao-chen, YUFeng, JIANGHong, SUNShu-long, GONGZheng-feng, XUJian-an
2014, 30(5): 458-460.
[Abstract](949) [PDF 1299KB](1631)
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OBJECTIVE To establish rabbit knee osteoarthritis models and to observe the degeneration of cartilage tissue and cartilage cells. METHODS 12 healthy male rabbits， weighing 2.0~3.0 kg， were randomly divided into sham operation group and model group， 6 in each group. Anterior cruciate ligament and medial collateral ligament were cut off in the right knee medial longitudinal incision and medial meniscus was resected， which was sutured by serial layers. 2×105 U of penicillin were intramuscularly injected daily for 7 days to prevent inflammation. The rabbits were forced to exercise for 60 minutes a day; after 12 weeks， they were killed and the complete knees were taken out as experimental samples. They were processed with morphological analysis and the two groups were conducted in cell culture to observe the morphological characteristics of rabbit chondrocytes. RESULTS The histological analysis was that safranine fast green staining in sham operation group showed articular cartilage surface in normal group was smooth， flat， evenly distributed， aligned and clear of all layers; model group showed articular cartilage surface cracked， some part impaired， cartilage cells significantly reduced， cartilage level dyed uneven， and even the local unstained. Chondrocytes cultured in sham operation group were mostly fusiform or short spindle with a small part polygonal and nuclei circular or oval; expression of type Ⅱcollagen significantly reduced. CONCLUSION Rabbit knee osteoarthritis models are successfully established and analyzed first with modern techniques. The model operates simply and has a good repeatability， which lays a foundation for further study of TCM preventing and treating knee osteoarthritis.
Influence of Chuanxiong Polysaccharide on Proliferation and Apoptosis of Human Hepatoma Cell HepG2
WANG Jing-chun, LIU Wei, YANG Rui-ling, SUN Xiao-bo
2014, 30(5): 461-464.
[Abstract](1764) [PDF 1210KB](1671)
Abstract:
OBJECTIVE To investigate the mechanism of Chuanxiong polysaccharide inhibiting proliferation and inducing apoptosis of human hepatoma cell HepG2. METHODS Human hepatoma cell line HepG2 were cultured in vitro， added with different concentrations of Chuanxiong polysaccharide. HepG2 cell activity was detected in MTT method; cell morphology was observed and HepG2 cell cycle was detected in flow cytometry. RESULTS HepG2 cell viability reduced with increasing concentration and the inhibitory effect showed a significant dose-dependent (P<0.05); compared with blank group， after 48h， percentage of cells in drug-administered group in G1 phase significantly increased (P<0.01) while the percentage of cells in S phase significantly reduced(P<0.01). CONCLUSION Chuanxiong polysaccharide can significantly inhibit human hepatoma HepG2 cells in vitro and inducing apoptosis by arresting tumor cells in G1 phase.
Study on Synthesis and Hepatoprotective Activities of Sauchinone Derivatives
XU Chun-lei, ZHANG Fang, CHEN Hong-jiang, Li Xiang
2014, 30(5): 465-467.
[Abstract](5024) [PDF 1109KB](2210)
Abstract:
OBJECTIVE To modify the structure of Sauchinone， an hepatoprotective ingredient， to obtain the derivatives with better hepatoprotective activity. METHODS Sauchinone was taken as the primary material and derivative A was obtained through C-2' hydroxyl reduction to be carbonyl with NaBH4 in tetrahydrofuran-methanol alkaline system; acetic anhydride taken as acetylating agent， DMAP as catalyst and pyridine as solvent， the hydroxyl was acylated to get derivative B. Hepatoprotective enzyme activity of derivatives was investigated with carbon tetrachloride-induced acute liver injury mice. RESULTS Saururaceae derivatives A and B were obtained unwritten in any literature， whose structures were confirmed through 1H NMR， 13C NMR and Q-TOF-MS. Pharmacological experiments showed that the lowering transaminase effect of A was better than that of Sauchinone while B has the same effect with Sauchinone. CONCLUSION The lowering transaminase effect can be enhanced through hydroxyl reduction to be carbonyl， which shows the same effect with Sauchinone after acylating.
The Protective Effects of Buxin Ruanmai Granules on Rats with Acute Myocardial Ischemia
ZHAOLing-gang, QIANYu-liang, XULi, YANDong
2014, 30(5): 468-470.
[Abstract](909) [PDF 1185KB](1248)
Abstract:
OBJECTIVE To explore the protective effects of Buxin Ruanmai Granules (BXRMG) on rats with myocardial ischemia caused by isoprenaline (ISO). METHODS The SD rat model of acute myocardial ischemia was developed by injecting isoproterenol hydrochloride (ISO) into subcutaneous tissue on the back and limbs. The effects of BXRMG on the displacements of ST region were observed at 1， 2， 3， 5， 10， 15， 20， and 30 mins after injection. Orbital blood was collected 30 minutes after modeling. Then， serum was separated and LDH and CK activities were determined and myocardial pathological morphology was observed. RESULTS The effects of BXRMG on ST region in myocardial ischemia induced by ISO indicated that LDH and CK level were improved significantly and histopathology was also improved. CONCLUSION Buxin Ruanmai Granules may protect the heart through strengthening the body's tolerance to ischemia， stabilizing the membrane and reducing the attacked areas from myocardial necrosis.
Pharmacokinetics of Curcuminoids-loaded Polyelectrolyte Nanoparticles in Rats
YANGLiu, LIUGui-hua, SONGJue, WANGXuan, PINGQi-neng, XIAOYan-yu, ZHANGCan
2014, 30(5): 471-474.
[Abstract](903) [PDF 1147KB](1254)
Abstract:
OBJECTIVE To investigate the pharmacokinetical process of curcuminoids-loaded polyelectrolyte nanoparticles (CUR-PENPs) by i.v. administration in rats. METHODS CUR-PENPs were prepared and characterized. The rats were administrated through tail vein with curcuminoids (CUR) solution and CUR-PENPs. RESULTS CUR-PENPs were quasi-spherical shapes observed by TEM. The mean particle size， Zeta potential and drug loading were (243.0 ± 1.1)nm， (-28.13 ± 1.41)mV and 5.5%， respectively. After i.v. administration the area under concentration-time curve (AUC) of CUR-PENPs were 3.30-fold higher than those of CUR solution， the clearance (CL) of CUR-PENPs were 27.8% that of CUR solution. CONCLUSION The results indicate that CUR-PENPs may extend the retention time of CUR and improve the AUC when compared with CUR solution.
Study on the Chemical Components of Fructus Ligustri Lucidi
NIEYing, YAOWei-feng
2014, 30(5): 475-477.
[Abstract](1138) [PDF 1129KB](1162)
Abstract:
OBJECTIVE To investigate the chemical components of Fructus Ligustri Lucidi. METHODS Isolated and purified the chemical components from the extracts of the petroleum ether and ethyl acetate part of Fructus Ligustrum lucidum by various chromatographic method. these compounds were identified by 1H-NMR， 13C-NMR、EI-MS(m/z). RESULTS The chemicals were: palmitic acid (Ⅰ)， physcion (Ⅱ)， lupeol (Ⅲ)， acetyl oleanolic acid (Ⅳ)， β-sitosterol (Ⅴ)， betulin (Ⅵ)， 19α-hydroxy-3-acetyl ursolic acid (Ⅶ)， oleanolic acid(Ⅷ)， ursolic acid (Ⅸ). CONCLUSION Compound 2 was first isolated from Fructus Ligustri Lucidi.
The HPLC Fingerprint of Green Forsythiae Fructus
CUIYu-wen, XUMiao-miao, YANGLi-bin, YANGJing, SONGXiao-mei, CUIJiu-cheng
2014, 30(5): 478-480.
[Abstract](899) [PDF 1178KB](1465)
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OBJECTIVE To establish HPLC fingerprint map of Green Fructus forsythiae. METHODS Chromatographic Column: Thermo C18 (250 mm×4.6 mm， 5 μm column); Column temperature: 30 ℃; Moving phase: acetonitrile-water， solution in gradient elution; Flow rate: 1.0 mL/min; The UV-detection wavelength: 203 nm. RESULTS The HPLC fingerprint map of Green Fructus forsythia has been established， with 21 common peaks marked， 3 of which is identified. CONCLUSION The established method is simple， accurate and rapid， which has provided reliable method for comprehensive evaluation of the quality of Green Fructus forsythia.
Study on Preparation Technology of Yangxue Ruanjian Tablet
SONGHan-min, LIYan-chao, LIYi-xian, YUANXiu-rong
2014, 30(5): 481-483.
[Abstract](859) [PDF 1043KB](1064)
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OBJECTIVE To study the formation and preparation technology of Yangxue Ruanjian Tablet. METHODS The single-factor method was taken to optimize the pharmaceutical formulations and the content was selected by granulating difficulty， angle of repose， degree of surface smoothness， hardness and disintegration. RESULTS Fluidized-bed granulating， tabletting and coating the mixture of raw powder， microcrystalline cellulose(MCC)， Sodium carboxymethyl starch and dextrin were made according to the proportion of 300: 50: 6: 3: 1. CONCLUSION Yangxue Ruanjian Tablet is reasonable and the process is simple and feasible， which conforms to the requirements for production.
Wang Zai-fang's Clinical Experience in Treating Contractile Failure with Yiqi Qiangxin Decoction
LI He
2014, 30(5): 484-485.
[Abstract](4874) [PDF 1028KB](2699)
Abstract:
This paper is to introduce Prof. Wang Zai-fang's clinical experience in treating contractile failure with Yiqi Qiangxin Decoction. Prof. Wang holds that the pathogenesis of this disease is due to the qi deficiency as its root cause and phlegm (dampness) stagnation as the excessive pathogen. It will attack the heart and pertain to the lung， spleen and kidney. Excess occurs during the attacking stage and deficiency becomes worse during the chronic stage. The basic principles for the treatment are reinforcing qi and warming yang as well as activating blood circulation and inducing diuresis. Yiqi Qiangxin Decoction is commonly applied in the clinic with certain modification.
Han Shuren's Clinical Experience of Treating Pulmonary Interstitial Fibrosis with Buyang Huanwu Decoction
WEI Yu
2014, 30(5): 486-488.
[Abstract](5267) [PDF 1047KB](3272)
Abstract:
This paper is to introduce Han Shuren's clinical experience of treating pulmonary interstitial fibrosis with Buyang Huanwu Decoction. Han believes the pathogenesis of this disease is the qi and blood deficiency， especially in lung and kidney and he adopts nourishing qi and tonifying kidney as well as activating blood circulation to remove blood stasis as his main treatment principle. He also modifies Buyang Huanwu Decoction according to different situations of the patients with different ages and disease courses in clinic.
Clinical Observation on Instant Antihypertensive Effect of Acupuncturing Taiyuan (LU9) in Treating 58 Cases with Primary Hypertension
DING Yu-mei, MA Xiao-yong
2014, 30(5): 489-491.
[Abstract](947) [PDF 1046KB](1008)
Abstract:
OBJECTIVE To observe the instant antihypertension effect of acupuncturing Taiyuan (LU9) in treating primary hypertension. METHODS Blood pressure of treatment group with 58 cases needed to be measured at 30 min， 40 min， 50 min， 60 min， 6 h and 24 h respectively， so as to observe the changes of blood pressure and side effect in different periods before and after acupuncture. The control group with 52 cases in it were treated with 10 mg sublingual nifedipine tablets and blood pressure needed to be measured at 30 min， 40 min， 50 min and 60 min respectively， so as to observe the antihypertension effect. RESULTS The comparison of two groups' diastolic pressure and systolic pressure before and after treatment was statistically significant(P<0.01)， as well as the comparison of antihypertension at 6 h and 24 h of two groups. There was no statistical significance of comparison of instant antihypertension ratio of two groups. CONCLUSION Compared with western medicine， acupuncturing Taiyuan (LU9) has two-ways regulation to antihypertension with sustained effect and fewer side effects. Decline legree of the blood pressure is positively correlated with blood pressure before acupuncture.
Clinical Study of Fufang Huangbai Liquid in Treating Acute and Sub-acute Aczema
LI Hui, GONG Juan-qin
2014, 30(5): 492-494.
[Abstract](941) [PDF 1051KB](1050)
Abstract:
OBJECTIVE To evaluate the efficacy and safety of Fufang Huangbai Liquid in treating acute and sub-acute aczema. METHODS 70 patients were randomly divided into experimental group and control group. Both groups were treated with compound triamcinolone acetonide cream， while experimental group were given Fufang Huangbai Liquid simultaneously for external use. Both drugs were given twice a day for two courses with two weeks in each. Clinical therapeutic effects and side effects in first and second week were observed. RESULTS Clinical efficiency was 88.9%（32/36）in experimental group， which was better than 67.6%（23/34）in the control group. There was an obvious difference between two groups（P<0.05）. Compared with control group， mean value of sign index of experimental group was distinctly lower， as well as the main symptoms. There was an obvious difference between two groups（P<0.05）. Lower frequency of drugs mainly manifested as transient stimulus response which can disappear in a short time. CONCLUSION Combination of steroid and glucocorticoid and Fufang Huangbai Liquid is safe and effective on treating acute and sub-acute aczema.
Treatment of Tongsaimai Tablet in 50 Cases Knee Osteoarthritis of Qi-Yin Deficiency
ZHANG De-xiong
2014, 30(5): 495-497.
[Abstract](924) [PDF 1050KB](1001)
Abstract:
OBJECTIVE To observe the therapeutic effect and safety of Tongsaimai Tablet in treating knee osteoarthritis (OA) of qi-yin deficiency type. METHODS 100 patients with knee osteoarthritis of qi-yin deficiency type were divided into treatment group equally assigned，according to randomizing number table，to the treatment group (50 cases) treated with Tongsaimai Tablet and the control group (50 cases) treated with glucosamine hydrochloride by random number table method. The indexes， which reflecting the degree of joint pain and walk capacity，pain and difficulty in up and down stairs， buckling angle and degree of stiffness， contracture， and swell， the improvement of patients symptoms and the integral estimation in patients， were measured and recorded before and after treatment. Moreover， blood， urinary and feces routine tests，liver and kidney function examination，and the adverse reaction that occurred in the treatment period were observed. RESULTS Indexes mentioned above in both groups before and after treatment showed significant statistical differences (P<0.05). Indexes in treatment group was more significant than that of the control group. No abnormal result was found， urinary and feces routine tests，liver and kidney function examination. CONCLUSION Tongsaimai Tablet is a safe and effective drug for the treatment of knee osteoarthritis，which could alleviate the joint pain，stiffness，dysfunction and swell.
Effects of Soy Isoflavones and High-Fat Diet on Genesis and Progress of 7， 12-dimethylbenz (α) Anthrancence-induced Breast Cancer in Young Female SD rats
DUPei, SHENHong-yi, LIZhong-ping, WUKe-jin, XIEYan, WANGLu, LIUXia
2014, 30(5): 498-500.
[Abstract](1090) [PDF 1223KB](1191)
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