2014 Vol. 30, No. 2

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On Tumor Differentiation and Treatment by TCM
ZHOU Zhong-ying, CHENG Hai-bo, ZHOU Xue-ping, WU Mian-hua, GUO Li-zhong, JIN Miao-wen, LI Liu
2014, 30(2): 101-104.
[Abstract](900) [PDF 1045KB](987)
Abstract:
Tumor differentiation and treatment by TCM is discussed in this paper and the academic thought of "cancerous toxin" has been raised based on the clinical practice of determining etiologic factors based on differentiation and treating tumor by ways of dispelling poison， detoxifying and counteracting toxic substances. Tumor is considered to be the result of the disorder of qi transformation of zang-fu organs， with phlegm and toxicity stasis as the core of the pathogenesis. Thus， syndrome and disease differentiation should be complementary and the development of evil qi and vital qi， the connection between tumor and the diseases should be fully considered. Detoxifying and counteracting toxic substances should be adapted to the patients' situation and the pathological features should be judged by the site of the tumor. Compound methods and complex prescription are proposed as the vital method to treat tumor and the key points of using medicines are mentioned in this paper. For example， relieving symptoms are of great importance and the recurrence and metastasis of tumor should be stopped and the most important thing is to nourish the vital qi.
Pathogenesis Theory of Cancerous Toxin in Tumor Microenvironment
CHENG Hai-bo, SHEN Wei-xing, WU Mian-hua, ZHOU Zhong-ying
2014, 30(2): 105-107.
[Abstract](1025) [PDF 358KB](1142)
Abstract:
The occurrence and development of tumor are closely related to the tumor microenvironment. The new strategy of treating malignant tumor is to interfere with the microenvironment to stop canceration. Professor Zhou， through years of clinical practices， have put forward the theory that cancerous toxin is the key to the occurring and developing of cancer. This research aims to discover the scientific connotation of cancerous toxin theory to provide scientific basis for using the theory to guide the treating of tumor in TCM by discussing the relation between pathogenesis theory of cancerous toxin and the tumor microenvironment.
Discussion on Zhang Zhongjing's Formulas Treating dysentery and diarrhea
GUAN Hua-quan, LI Fei
2014, 30(2): 108-110.
[Abstract](866) [PDF 1031KB](1258)
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Zhang zhongjing's different prescriptions on the treatment of cold， heat， deficiency and excess syndromes are summarized in the paper. For example， heat syndromes are treated by Pulsatilla decoction and Scutellaria decoction; excess heat is treated by Da Chengqi Decoction or Xiao Chengqi Decoction; cold syndromes are treated by Sili decotion; deficiency syndromes are treated by the Lizhong pill or Halloysite Limonitum decotion .Through the analysis of the above formulas together with clinical experiences， the compatibility rules are summarized for clinical reference.
Effect of Acupoint-massage on the Inflammatory Cytokines Levels of Serum in Obese Type 2 Diabetic Patients
WANGZhao-hui, QIWei, HANDong-yue, SONGBai-lin, WANGZhi-hong
2014, 30(2): 111-113.
[Abstract](870) [PDF 1035KB](1033)
Abstract:
OBJECTIVE To observe the effect of acupoint-massage on the inflammatory cytokines levels of serum in obese type 2 diabetic patients. METHODS 75 obese type 2 diabetic patients with damp-heat in the spleen were randomly divided into treatment group (control group with abdominal massage therapy) and control group (treated with metformin hydrochloride tablets). The inflammatory cytokine interleukin6， tumor necrosis factor alpha， and inflammatory cytokines C reactive protein were compared before and after the treatment. RESULTS Levels of serum inflammatory factors of IL-6 TNF-α， CRP were decreased. CONCLUSION The effect of acupoint-massage combining with metformin is better than using metformin alone in patients with type 2 diabetes obesity.
Observation of Zhuang Medicine Tazhong fumigation-washing form Combined with Urokinase Thrombolysis on Acute Deep Venous Thrombosis of Lower Limbs
DU Juan-jiao, ZHANG Li, HUANG Xin, LI Jie-hui
2014, 30(2): 114-116.
[Abstract](823) [PDF 1034KB](1016)
Abstract:
OBJECTIVE To observe the clinical effect of Zhuang Medicine Tazhong fumigation-washing form combined with urokinase thrombolysis for acute deep venous thrombosis (ADVT) of lower limbs. METHODS 100 cases of ADVT patients were randomly divided into A group (50 cases) treated with urokinase thrombolysis alone and B group (50 cases) treated with Zhuang Medicine Tazhong fumigation-washing form additionally. 2 weeks made up one course of treatment. RESULTS Tazhong fumigation-washing form combined with urokinase thrombolysis can alleviate the swelling of limbs， relieve pain and lower fibrinogen combined with A group. CONCLUSION Combination of traditional Chinese and Western medicine has a significant effect on ADVT patients.
Clinical Observation of Tongtai Decoction Combined with Chemotherapy in Treating Advanced Colorectal Cancer
XUYou-qi, QIANJuan, WANGQian
2014, 30(2): 117-119.
[Abstract](902) [PDF 1035KB](1130)
Abstract:
OBJECTIVE To observe the clinical effects of Tongtai decoction combined with chemotherapy in treating advanced colorectal cancer. METHODS 40 cases were randomly divided into two groups: control group and treatment group with 20 cases in each. Treatment group was given Tongtai decoction combined with chemotherapy at the same time while control group was given chemotherapy only. We observed the clinical efficiency， change of tumor marker CEA， integration changes of symptoms， clinical symptoms before and after treatment， karnofsky score， adverse reactions and tolerability of chemotherapy. RESULTS The patients of two groups have shown no significant difference in objective curative effect and in reducing the value of serum CEA (P>0.05) . But the treatment group was superior to the control group in reducing the toxicity of chemotherapy，improving the clinical symptoms， improving quality of life and the general condition (P<0.05). CONCLUSION The combined treatment of Tongtai decoction with chemotherapy can effectively resolve clinical symptoms of patients with advanced colorectal cancer， lower toxicity during chemotherapy， and improve their quality of life.
Study on the Curative Effect of Thermal Moxibustion Treating Lumbar Disc Herniation
FUYong, ZHANGHai-feng, XIONGJun, ZHANGWei, ZHOUXiao-ping, XUHan-bin
2014, 30(2): 120-123.
[Abstract](935) [PDF 1058KB](1140)
Abstract:
OBJECTIVE To compare the differences of curative effect among thermal moxibustion， traditional moxibustion and conventional Western medicine with acupuncture in the treatment of Lumbar Disc Herniation. METHODS 180 cases were randomly divided into 3 groups. A group: Applying thermal moxibustion therapy to the inside region of Dachangshu-Yaoshu (Du 2)-Dachangshu on the contralateral for treatment. B group: Using traditional moxibustion therapy to cure by selecting the acupuncture points in the above regions. C group: Employing the method of the conventional Western medicine with acupuncture. All groups were treated for two weeks， in total of 18 times. Observing the therapeutic effect by using the modified lumbago rating scale from the Japanese Orthopaedic Association in both periods: before treatment， the end of treatment and last for another six months. RESULTS After treatment， there is a significant difference between the 3 groups. In the aspects of improving lumbago and dysfunction， the group of thermal moxibustion are obviously better than the group using traditional moxibustion and control group applied the method of conventional Western medicine with acupuncture (P<0.05). After 6 months' observation， the advantages of curative effect was still obviously in group with thermal moxibustion. CONCLUSION The curative effect of thermal moxibustion is significant compared with traditional moxibustion (P<0.05) and conventional Western medicine with acupuncture (P<0.05).
Effects of Integrative Chinese and Western Medicine on Blood Glucose and Renal Function and Quality of Life in Patients with Type 2 Diabetic Nephropathy
ZHOULi-li, LOUXiao-jia
2014, 30(2): 124-126.
[Abstract](899) [PDF 1036KB](1063)
Abstract:
OBJECTIVE To investigate the effects of integrative Chinese and western medicine on blood glucose， renal function and quality of life (QOL) in patients with Diabetic nephropathy DN in order to supply strategy for the treatment of early DN. METHODS 124 cases with early DN were randomly divided into western medicine group (n=62) and integrative group (n=62). The former were treated with western medicine and the latter were given Chinese medicine additionally for 3months. The levels of FPG， 2hPG， SUN， SCr and 24 h urine albumin quantitative and adverse reaction were evaluated in both groups and the QOL were measured by SF-36Scale. RESULTS The levels of FPG， 2hPG， SUN， SCr， and 24 h urine albumin quantitative were all significantly decreased after 3-month treatment than those in baseline (P<0.05) of which only SUN， SCr， and 24 h urine albumin were showed significant differences between the two groups(P<0.05). The incidence of adverse reaction was 20 97% in western medicine group， higher than 11.29% in integrative group (P<0.05) The scores in 8 fields of SF-36 Scale were all increased than baseline (P<0.05)， but the integrative group changed more (P<0.05). CONCLUSION Integrative Chinese and western medicine can obviously improve the patient's kidney function， reduce urinary protein， improve the patient's quality of life and reduce the adverse reaction in the treatment of early DN.
Clinical Study on Ear Treatment Combined with Self-management Intervening 99 Patients with Hypertension
CHEN Jing-wei, LIN Xiao-li, ZHAO Jing-ying, ZOU Tao
2014, 30(2): 127-129.
[Abstract](885) [PDF 1035KB](1049)
Abstract:
OBJECTIVE To evaluate the curative effect of ear treatment combined with self-management on blood pressure and the TCM clinical symptoms of the hypertensive patients. METHODS 199 hypertensive patients were randomly divided into the control group given conventional treatment and the treatment group given ear treatment combined with self-management additionally. 20 weeks made up one course of treatment. Evaluating the curative effect by the dynamic changes of blood pressure and TCM clinical symptoms score. RESULTS The SBP and DBP of patients in treatment group were significantly lower compared with control group (P<0.05). In terms of the TCM clinical symptoms， there was a remarkable difference (P<0.05) too. CONCLUSION Ear treatment combined with self-management can lower blood pressure， improve the TCM clinical symptoms as well as the life quality of hypertensive patients.
Regulation of Th17/Treg by Qingchang Huashi Fang on Trinitrobenzene Sulfonic Acid induced Colitis in Mice
LUYue-lin, SHENHong, YAOHong-feng, YANGXu
2014, 30(2): 130-133.
[Abstract](988) [PDF 1712KB](993)
Abstract:
OBJECTIVE To observe possible mechanism of the Qingchang Huashi Fang's therapeutic effect on Ulcerative Colitis (UC) through regulating Th17/Treg on trinitrobenzene sulfonic acid induced colitis in mice. METHODS Mice successfully modeled with TNBS were divided into blank model， Qingchang Huashi Fang (12.8 crude drug kg/d) and SASP (0.5 g/kg) groups. All Mice， single administration for 7 days， were executed to collect colon tissues and cut out for related inspections. The expression of IL7 and Foxp3 in colonic epithelium was checked up with immunohistochemical method， and measure RORγt and Foxp3 protein in colonic epitheliumand by western blotting method. RESULTS The expression of IL7 in colonic tissues of Qingchang Huashi Fang group were lower than those in blank model group (P<0.05)， and the expression of RORγt protein was significantly lower than those in blank model group (P<0.01). Foxp3 protein was higher than those in model group (P<0.05). CONCLUSION Qingchang Huashi Fang can inhibit Th17 cell differentiation and IL7 generation through lower down egulation RORγt. It can promote Treg cell expression through regulation Foxp3. Qingchang Huashi Fang can effectively inhibit inflammation in TNBS induced colitis in mice through regulation Th17 Treg balance.
The Effect of Layers Adjusting External Application Therapy on Matrix Metalloproteinases in Synovial Membrane in the Rabbit Knee Osteoarthritis
MAOJun, ZHANGLi, ZHAOLin-rui, DINGLiang, WANGPei-min
2014, 30(2): 134-137.
[Abstract](797) [PDF 1209KB](976)
Abstract:
OBJECTIVE To investigate the effect of Layers Adjusting External Application Therapy on the expression of synovial inflammatory factors and matrix metalloproteinase (MMPs) in osteoarthritis (OA). METHODS 30 New Zealand rabbits were randomly divided into normal group， control group and treatment group. OA model was induced in rabbit by unilateral posterior anterior cruciate ligament transection (ACLT). 6 weeks post-operation， the Layers Adjusting External Application Therapy were used externally; 8 weeks post-operation， the rabbits were sacrificed. The synovial membrane specimens were taken to detect the expression and content of IL-1β， MMP-1 and MMP-13 by Western Blot and Fluorogenic Quantitative PCR. RESULTS Western Blot: The content of MMP-1 and MMP-13 in the control group were significantly higher than the treatment group. The content of MMP-1 and MMP-1 in the normal group were the lowest Fluorogenic Quantitative PCR. The expression of MMP-1 and MMP-13 in the control group were the highest the expression of MMP-1 and MMP-13 in the normal group were the lowest the expression of IL-1β in the normal group was significantly lowest. There were no significantly differences in the expression of IL-1β between control group and treatment group. CONCLUSION Layers Adjusting External Application Therapy can effectively inhibit the expression of MMP-1 and MMP-13 in the Synovial Membrane， thus reduces the degradation of cartilage matrix alleviate the progression of OA. Nevertheless， further research are needed to investigate the specific target so that the mechanism of the therapy can be revealed.
The Effects of Pricking Blood Therapy on Articular Cartilage Ultrastructure of Acute Gouty Arthritis Model
LIANGSha, XIAYou-bing, CHENGJie, MUYan-yun, GUOJing, XIAOAn-ju, LYUKai-lu, ChouShan-bo
2014, 30(2): 138-141.
[Abstract](899) [PDF 2242KB](961)
Abstract:
OBJECTIVE To observe the effects of pricking blood therapy on local symptoms of the acute gouty arthritis rat model， morphological changes and ultra-structure， and to explore the effective mechanism of pricking blood therapy. METHODS Forty SD rats were randomly divided into control group， model group， drug group and pricking blood group. The acute gouty arthritis model was set by injecting uric acid into the right ankle joint cavity of the rats. The drug group received 120 mg/kg gastric perfusion of ibuprofen; the pricking blood group pricked the right Kunlun points. The diameter of the injected right ankle joint of the forty rats were measured and recorded before modeling， and 6 h， 24 h， and 48 h after the rats were modeled. The morphological changes were observed under light microscope and ultra-structure was under electron microscope. RESULTS For modeling after 24 hours， there were significant statistical differences between pricking blood group and drug group， and between pricking blood group and model group (P<0.05)， while there was no significant statistical difference for modeling after 48 h (P>0.05). From light microscope， sodium urate crystal deposition had been found in model group with dense inflammation infiltration; there were also some sodium urate crystal and inflammation infiltration in drug group; small amount of pink crumby deposition and inflammation infiltration were found in pricking blood group. As shown in electron microscope， for model group， a few of articular cartilage cell membrane was damaged. Inflammation infiltration occurred and cell process endopasmic reticulum expansion turned up with the same occasion for drug group and arrangement of collagen fibers was disordered and dense. For drug group， mitochondria decreased and arrangement of collagen fibers was disordered. For pricking blood group， there was slight disorder in collagen fibers and cell structure was normal. CONCLUSION Pricking blood therapy may reduce the deposition of uric acid sodium in articular cavity， lessen infiltration of the inflammatory cells， and improve the ultra-structure of arthrodial cartilage rapidly and effectively. Compared with conventional therapy， pricking blood therapy has a better effect， and works quickly with less side-effect.
Effect of Electroacupuncture on Content of hs-CRP and IL-6 in ApoE-/- Mouse Plasma and Expression of TNF-α in Arotic Sinus Plaques
ZHANDong-mei, TAOWen-jian, AIBing-wei
2014, 30(2): 142-145.
[Abstract](913) [PDF 1549KB](920)
Abstract:
OBJECTIVE To observe the effect of electroacupuncture on the content of high sensitive C-reactive protein (hs-CRP) and interleukin-6 (IL-6) and the expression of tumor necrosis factor-a (TNF-α) in aortic sinus plaques in ApoE-/- mice plasma. METHODS 30 successfully-modeled ApoE-/- mice were randomly divided into three groups as model group， drug-control (DC) group and electro-acupuncture (EA) group， with 10 mice in each group. EA group was treated with acupuncture in Shenmen， Neiguan， Housanli and Sanyinjiao， connecting with the Hans electroacupuncture device. Meanwhile， the gavage administration of 40mg/kg.d simvastatin was adopted in DC group. The content of hs-CRP and IL-6 in mouse plasma was detected by ELISA and the expression of TNF-α was by immunohistochemistry (IHC). RESULTS Compared with model-group， the content of hs-CRP and IL-6 in EA group obviously decreased (P<0.01); IHC showed that there was plenty of TNF-α of model group distributed on fibrous cap and a large scale of necrotic core and cholesterol crystals was found in aortic sinus plaques; while in EA group， there was little TNF-α on fibrous cap without significant necrotic core and cholesterol crystals. CONCLUSION Electroacupuncture treatment can protect vessel endothelium and inhibit the development of plaques by improving the mice body's inflammatory response state， indicating that anti-inflammatory effect may be one of the mechanisms of AS treatment with electroacupuncture.
The Protective Effect of Tongsaimai Decoction on PC 12 Cells Induced by H2O2
MaxwellKimKitLee, DILiu-Qing, LIJun-song, BIXiao-lin
2014, 30(2): 146-149.
[Abstract](862) [PDF 1063KB](1003)
Abstract:
OBJECTIVE To observe the protective effects of Tongsaimai decoction preparation on the PC12 cells induced by H2O2. METHODS The in-vitro cultured PC12 cells were randomly divided into eight groups such as normal group， hydrogen peroxide group， Tongsaimai decoction with high dose group(5 mg/mL)， Tongsaimai decoction with middle dose group(0.5 mg/mL)，Tongsaimai decoction with low dose group(0.05 mg/mL). After the 200 μmol/L H2O2 was added to stimulate the PC12 cells and incubated for 4 h， then the final concentration of drugs (5 mg/mL， 0.5 mg/mL， 0.05 mg/mL) were added and continuously incubated and cultured for 24 h. The MTT assay was facilitated to measure and calculate the cell viability the protection rate of the drugs on PC 12 cells induced by hydrogen peroxide. Finally， the corresponding biochemical indicators like SOD， MDA，NO， LDH and Ca2+ were measured. RESULTS The Tongsaimai decoction(5 mg/mL， 0.5 mg/mL， 0.05 mg/mL) possess protective effects on H2O2-cellular injury model group induced to PC12 cells injury. The hydrogen peroxide model group revealed that could reduce the SOD activity and SOD inhibition rate(P<0.05~0.01)， conversely could increase the MDA， NO content， LDH activity and Ca2+ levels(P<0.05~0.01).Each dose of drugs groups could increase the SOD activity and SOD inhibition rate， conversely could reduce the LDH activity， NO content， MDA and Ca2+ levels when compared with the hydrogen peroxide model group showing the significant differences(P<0.05~0.01). CONCLUSION Tongsaimai decoction has revealed the significant protective effects towards the PC12 cells injury， its mechanisms are closely-related to the calcium overload， the oxidative stress and others.
Therapeutic Effects of Danggui Buxue Decoction and its Decomposition on Rats with Immunological Liver Injury
SUNLi-xia, FANGNan-yuan, ZHOULing-Ling, LIUZhang-pu
2014, 30(2): 150-152.
[Abstract](883) [PDF 1049KB](1056)
Abstract:
OBJECTIVE To observe effects of Danggui Buxue Decoction and the composition on liver function and liver fibrosis in rats with immunological liver injury. METHODS The immunological liver injury models were established with intraperitoneal administration of pig serum， whose alanine aminotransferase (ALT)， aspartate aminotransferase(AST)， superoxide dismutase(SOD)， lipid peroxide malondialdehyde (MDA)， Hyaluronic acid (HA)， IV collagen and III procollagen were detected. RESULTS The group with ratio of Astragalus root to Chinese angelica 5:1 significantly decreased AST and ALT levels; Astragalus root group， Chinese angelica group and groups with different ratios of Astragalus root to Chinese angelica increased SOD levels and decreased MDA content; Astragalus root group obviously reduced HA and type-IV collagen; Danggui Buxue Decoction group with the ratio 5:1 significantly reduced type-III procollagen levels， superior to that in group with ratio10:1. CONCLUSION Danggui Buxue Decoction makes a certain effect on rats with immunological liver injury， whose mechanism may be related to reduction and inhibition of lipid peroxidation and anti-free radical damage.
Experimental Study of Shuilu Erxian Pill in the Treatment of Rats with Deficiency Syndrome
HUYing, WUQi-nan, YUEWei, GUWei, WUCheng-yin
2014, 30(2): 153-155.
[Abstract](1017) [PDF 1036KB](1117)
Abstract:
OBJECTIVE To study the effect of the Shuilu Erxian Pill on the spleen indexes of rats with deficiency syndrome by pharmacological experiments as well as the influence on GAS， SS， MTL， MS， LDH and SDH in serum， in order to explore the mechanism of Shuilu Erxian Pill for treating deficiency syndrome. METHODS Water， 95% ethanol extraction and processed samples from process of waste solution were prepared for studying the effect of the samples on rats with deficiency syndrome due to dietary disorder. RESULTS the model rats with deficiency syndrome were lower than that of the control group. GAS， SS， MTL， AMS and LDH levels in serum were significantly lower， and SDH content was also higher compared with control group. GAS， SS and MTL and AMS， LDH content in the serum of rats of the Shuilu Erxian Pill water extraction high-dose group， middle-dose group and small-dose group were higher than that of model group， while SDH was lower than that of the control group. There were significant differences in water extraction high-dose group and middle-dose group compared with the model group. Though GAS， SS and MTL， AMS，LDH content in rats serum of high-dose group and middle-dose group of the Shuilu Erxian Pill extraction were slightly higher than the model group and SDH content was slightly lower， there was no significant difference among three groups， compared with the model group. CONCLUSION The effective components of Shuilu Erxian Pill for deficiency syndrome were water-soluble. The mechanism of Shuilu Erxian Pill for deficiency syndrome was related to the increasing of spleen index， GAS， SS， MTL， AMS， and LDH， and the reduction of SDH. The effective components had a better effect compared with alcohol extraction.
Effects of 5-Fu on Mice with Spleen Deficiency Syndrome
GUOJin-rui, GEFei, KANGAn, WANGXiao-long
2014, 30(2): 156-159.
[Abstract](980) [PDF 1199KB](1000)
Abstract:
OBJECTIVE To study the effects of 5-Fu on mice with spleen deficiency syndrome and the content of short chain fatty acid in intestine. METHODS After the injection of 5-Fu in abdomen， data such as food intake， body weight changes， diarrhea and other information were collected to observe symptoms due to spleen deficiency. Endo-intestinal toxicity of 5-Fu were evaluated by HE staining and detecting beta-D-galactose glucoside enzyme activity in intestinal mucosa at the same time. The content of short chain fatty acids in intestinal contents were also determined to investigate the influences of 5-Fu on the intestinal flora. RESULTS Compared with control group， the spleen deficiency syndrome may induced by 5-Fu， the mice appeared the symptoms， such as poor appetite， weight loss and diarrhea， and it was shown in the physiological section that the intestine villis significantly shortened， the pathological changes further developed， the contents of TNF-α and IL-6 in plasma increased and the beta-D-galactose glucoside enzyme activity of intestinal mucosa decreased. Contents of acetic acid， butyric acid and pentanoic acid in intestine significantly reduced at the same time. CONCLUSION Fu may induce spleen deficiency syndrome in mice and significantly damage the intestinal mucosa and intestinal flora， which indicate that when 5-Fu is applied， some spleen-strengthening medicine may be used to reduce induced intestinal side effects.
Study on Absorption Characteristics of Calycosin in Single-pass Intestinal Perfusion Models in Vivo
ZHOULe, ZHAOXiao-li, DILiu-qing, CHENYao
2014, 30(2): 160-163.
[Abstract](954) [PDF 1091KB](1243)
Abstract:
OBJECTIVE To study the dynamic characteristics of intestinal absorption of calycosin using signal-pass perfusion model in rats. METHODS After one-way intestinal perfusion model of rat in situ was set up，by taking phenolsulfonphthalein a maker，the absorption characteristics of different intestinal segments. RESULTS Calycosin have a good stability separately in different intestinal segments and in the colon to absorb the best. Different concentrations of drug in the colon absorption in inhibition of its concentration. CONCLUSION Calycosin is well absorbed in the small intestine and calycosin absorption may active transport.
Study on Phospholipid Complex Improving Oral Danshensu Bioavailability
LIUSha-sha, LIWen, LIJun-song, DILiu-qing
2014, 30(2): 164-167.
[Abstract](918) [PDF 1246KB](1008)
Abstract:
OBJECTIVE To explore pharmacokinetic features of Danshensu phospholipid complex（DPLC） in rats and compare with Danshensu APIs. METHODS Danshensu and DPLC (containing the same amount of Danshensu) were taken in intragastric administration.The concentration of Danshensu in plasma was detected in LC-MS/MS method while pharmacokinetic parameters were calculated with DAS 2.1.1 software. RESULTS In Danshensu group， C max was（1.28±0.13）×103 ng/mL，T max was (51±8.22) min，AUC 0-t was （2.05±0.24）×105  ng/(mL·min)；in DPLC group， C max was （1.88±0.07）×103 ng/mL，T maxwas (90±18.37) min，AUC 0-t was （3.07±0.50）×105 ng/(mL·min). CONCLUSION Phospholipid complex can increase oral Danshensu bioavailability.
Study on Preparation of 9-Nitrocamptothecin Stealth Liposomes Composed of DPPC and SPC
GUWei, CHENJun, QIAOGuang-jun, ZHANGYa-ping, ZHANGQian, YANXu, HURong-rong, YANGXi-xiong
2014, 30(2): 168-172.
[Abstract](921) [PDF 1586KB](1048)
Abstract:
OBJECTIVE To investigate the preparing methods and process of 9-nitrocamptothecin (9-NC) stealth liposomes composed of HSPC and SPC and to compare the pharmaceutical properties between 9-NC liposomes and 9-NC stealth liposomes. METHODS Encapsulation efficiency determination method for 9-NC stealth liposomes was established by ultrafiltration membrane method. The preparation for 9-NC stealth stealth liposomes was carefully studied and the process parameters were optimized. Furthermore， compared with 9-NC liposomes composed of HSPC and SPC without stealth-modified， encapsulation efficiency， particle size， zeta potential and polydispersity lactone stability were investigated. RESULTS The encapsulation efficiency of 9-NC stealth liposomes preparation by film-ultrasonic method was significantly improved， compared to those by ethanol injection method， and the optimized process parameters were HSPC:SPC=1:9. The formation time was 100min and formation pressure was 0.095 Mpa. Though there was on influence on the exception of encapsulation efficiency （80%） and zeta potential， particle size and lactone stability were significantly optimized by modified stealth materials. CONCLUSION Samples prepared by optimized method and process obtained high encapsulation efficiency and met the needs of drug effects in vivo studies; modified stealth materials may have effect in enhancing Lactone stability of 9-NC stealth liposomes composed of HSPC and SPC.
Wang Canhui's Experiences on Treating Difficult Diseases from Heat Stagnation and Blood Stasis Due to Qi Deficiency
ZHANGChun-rong, LIUTao
2014, 30(2): 173-175.
[Abstract](869) [PDF 1037KB](999)
Abstract:
Combined with cases of difficult diseases， professor Wang Canhui's experiences on treating difficult diseases with replenishing qi， clearing heat and activating blood are introduced. For pathogenesis of difficult diseases， professor Wang considers that qi deficiency is the main pathological basis， and heat stagnation and blood stasis are the important pathogenic factors. The three factors are reciprocal causation and their pathogenic changes influencing each other lead to prolonged of disease course.
Professor You Songxin Differentially Treating 4 Cases of Liver Cirrhosis Blood Syndrome of Hepatitis B
SHI Li-wen
2014, 30(2): 176-179.
[Abstract](878) [PDF 1050KB](1077)
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You Songxin's clinical experiences on treating liver cirrhosis blood syndrome of chronic viral hepatitis B( hepatitis B ) are introduced. Professor You considers that patients of liver cirrhosis with hemorrhage are usually treated from Ying and blood syndrome of epidemic febrile diseases， and the major prescription is Xijiao Dihuang Dection combined with SI Wuzeigu and Yi Luru Pill. But for women patients during child-bearing period with normal liver function but large quantity of menstruation， treatment of nourishing blood to regulate menstruation is applied and Yimu Sheng Jin Fang as major prescription is modified flexibly. When hemorrhage of patients is gradually stopped and the syndrome is transferred from root excess as main factor to deficiency and excess syndrome of damp stagnation due to yin damage， treatment of nourishing yin to clear heat is used for prognosis and the major prescription is Lankou Fengzhe decoction.
Professor Deng Yunming's Experience on Treating Gout in the View of Spleen-Stomach Differentiation
LIHua-nan, LIUFeng, TUHong, WANGLi
2014, 30(2): 180-182.
[Abstract](900) [PDF 1031KB](1177)
Abstract:
In this paper， the author introduced Deng Yunming's experience on treating gout， like the pathogenesis， treatment principle， stages treatment， prevention and recuperation. In Deng's view， the main pathogenesis of gout is disharmony of spleen and stomach， also caused by the stagnation of damp heat. The treatment focused on the relationship among liver， spleen and kidney. He also emphasized on the importance of prevention.
Comprehensive Study on the Influence of Compendium of Materia Medica on Japan and Korea's Medical Field
SHEN Chen, CHEN Wei-ping
2014, 30(2): 183-185.
[Abstract](932) [PDF 1029KB](1447)
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This paper introduced the transmission and influence of Compendium of Materia Medica on East Asia's medical field. Obviously Compendium of Materia Medica impact the classification of herbalism in Japan and Korea. In Japan， the researchers lay particular stress on the verify and identify of drugs， and in Korea， they emphasis the editing and simplification of the book based on the principle of simply and practical.
Clinical Study on Huangqi Guizhi Wuwu Decoction Treating the Neuro-sensory Toxicity Caused by Oxaliplatin
LI Dao-ming, WANG Rong, XIE Jing
2014, 30(2): 186-188.
[Abstract](1046) [PDF 1043KB](1334)
Abstract:
OBJECTIVE To observe the curative effect of Huangqi Guizhi Wuwu Decoction in the treatment of neuro-sensory toxicity caused by oxaliplatin. METHODS 48 cases of colorectal cancer were randomly divided into the control group (24 cases) treated with chemotherapy alone and the treatment group (24 cases) treated with Huangqi Guizhi Wuwu Decoction additionally. The neuro-sensory toxicity caused by oxaliplatin was judged by Levi standard. RESULTS Among 24 cases in treatment group， 8 cases presentedⅠclass， 1 patientⅡclass， and 0 patients Ⅲ / Ⅳclass， the total occurrence being 79.17%. In the control group， 13 cases presentedⅠclass， 3 patientⅡclass， 3 patients Ⅲ class and 0 case Ⅳclass. Compared with control group， there were less patients got Ⅲ/Ⅳ class toxic reaction in treatment group， which suggested a significant difference (P<0.01). CONCLUSION Huangqi Guizhi Wuwu Decoction has a significant effect on the neuro-sensory toxicity caused by oxaliplatin.
Heat-sensitive Moxibustion and Head Needle Acupuncture in Treating Insomnia
XU Hai-yan
2014, 30(2): 189-191.
[Abstract](820) [PDF 1038KB](1006)
Abstract:
OBJECTIVE To observe the clinical effect of heat-sensitive moxibustion and head needle acupuncture in the treatment of insomnia. METHODS 142 patients with insomnia were randomly divided into three groups: 30 cases in positive drug group， 54 cases in head needle acupuncture group， 58 cases in heat-sensitive moxibustion and head needle acupuncture group. Patients from positive drug group took estazolam 2.0 mg orally before sleep every night. Patients from head needle acupuncture group had acupuncture on top and forehead area once a day. In the heat-sensitive moxibustion and head needle acupuncture group， we carried out moxibusion at Guanyuan， Yongquan， Xinshu， Dazhui once a day， six days a week for four weeks. Insomnia index scores and the rate of sleep were recorded before and after treatment for statistical analysis. RESULTS After treatment， three groups of insomnia index scores decreased significantly with sleep rate increased (P<0.05~0.01). The effect of heat-sensitive moxibustion and head needle acupuncture group is the same as that of the positive drug group and is better than that of the head needle acupuncture group (P<0.05). CONCLUSION The heat-sensitive moxibustion and head needle acupuncture can significantly improved insomnia sleep for patients.
Animal Experimental Study on the Antidepressant Effects of Six Tranquilizing Medicines
DAI Jian-guo, WANG Zhong-li, CHEN Lin, ZHAO Yu-nan, HUANG Yu-fang
2014, 30(2): 192-194.
[Abstract](898) [PDF 1364KB](1213)
Abstract:
OBJECTIVE To explore the antidepressant effect six tranquilizing medicines， namely Acorus tatarinowii， Ziziphus jujuba， Zinnober， Thinleaf Milkwort Root-bark， Poria cum Radix， and Silktree Albizziae Flower. METHODS The effects of six medicines on spontaneous activity of mice were evaluated by field test and the effects on immobility time of mice were evaluated by forced swimming test and tail suspension test. The expression of glial fibrillary acidic protein was detected by western blot. RESULTS Compared with control group， no significant difference was found in total animal activities path in field test for each group (P>0.05). However， in the forced swimming test and tail suspension test， the immobility time of mice of Acorus tatarinowii， Zinnober， Thinleaf Milkwort Root-bark， Poria cum Radix， and Silktree Albizziae Flower group was both significantly reduced (P<0.05~0.01). There was no significant difference in expression of GFAP protein for each group (P>0.05). CONCLUSION Acorus tatarinowii， Zinnober， Thinleaf Milkwort Root-bark and Poria cum Radix have antidepressant effect.
Determination of Astragaloside Ⅳ and Calycosin-7-O-β-D-glucopyranoside in Qishen Oral Liquid by HPLC
HU Kun
2014, 30(2): 195-196.
[Abstract](896) [PDF 1076KB](1050)
Abstract:
OBJECTIVE To develop a HPLC method for the determination of astragaloside Ⅳ and calycosin-7-O-β-D-glucopyranoside in Qishen oral liquid. METHODS The astragaloside Ⅳ was determinated by ELSD with C18 column. Acetonitrile-water (ratio-32∶68) was set as the mobile phase， the temperature of drift tube was 102 ℃ and the gas flow rate was 2.7 L/min. DAD was used to detect calycosin-7-O-β-D-glucopyranoside， and the mobile phase was composed of acetonitrile and 0.2% formic acid aqueous for gradient elution with the detecting wave length of 260 nm. RESULTS The linear ranges of astragaloside Ⅳ and calycosin-7-O-β-D-glucopyranoside were 1.1～16.5 μg and 0.038～0.382 μg respectively. The average recoveries of two ingredients were 96.73% (RSD=2.08%) and 100.70% (RSD=3.12%). CONCLUSION The analytic results determined by HPLC were accurate， which can be used as reference for quality control in astragalus preparation.
Research progress on the Metabolomics in the Traditional Chinese medicine (TCM) and its compound
MAOYan-cao, SHANJin-jun, WUHao, XIETong
2014, 30(2): 197-200.
[Abstract](933) [PDF 1073KB](1125)
Abstract:

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