南京中医药大学学报

Study on Principle and Method of Integrated Traditional and Western Medicine

LIU Shen-lin

2014, 30(1): 1-3.

[Abstract](673) [PDF 1001KB](799)

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Re-understanding of Eight Liao Points

WANG Ling-ling, JIN Xun

2014, 30(1): 4-7.

[Abstract](1569) [PDF 1023KB](3836)

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According to clinical advantages showed in deep-needling eight liao points to treat pelvic floor diseases in recent years， difficult issues of eight liao points in locating and deep-needling are analyzed. It points out re-understanding eight Liao points， including their clinical effects and key techniques in manipulation. It introduces location method of eight liao points and points out that eight liao points having different needling angles is the key to make head of needle enter posterior sacral foramina smoothly when deep needling eight liao points.

The Primary Exploration of Dissolving rotten to Regeneration Method and the Vascularization of Jin Treating Diabetic Foot

XU Qiang, ZHANG Zhao-hui, MA Jing, ZHU Chao-jun, LIU Xian-zhou

2014, 30(1): 8-10.

[Abstract](668) [PDF 1011KB](747)

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The recovery of the gangrene on diabetic foot is closely related to tendon. The treatment of the degenerated and necrotic tendon is directly related to the preservation of the affected extremity. In clinical research， the new vessels on the basal part of the wound grow into tendon and adhere to it so that the granulation tissue wraps up tendon there by reversing the extended infection on the diabetic foot along tendon and promoting the wound healing， which is called the vascularization of the chronic wounded tendon. As tendon belongs to the category of Jin in traditional Chinese medicine， here we call the whole process as the vascularization of Jin. Under the guidance of vascularization of Jin， dissolving rotten to regeneration Method was developed as the therapy of traditional Chinese medicine.

Discussion on Characteristics of Ma Pei-zhi's Applying Surgical Knife-needle Clinically

LU Ye, WU Ya-xu

2014, 30(1): 11-12.

[Abstract](672) [PDF 983KB](931)

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Study the View of Balance in Treatise on Blood Syndromes

LV Jun

2014, 30(1): 13-14.

[Abstract](677) [PDF 986KB](745)

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Meta-analysis on the Randomized Controlled Trial of TCM Drugs Treating on Children's Upper Respiratory Tract Infection with Fever

QIUJian-li, XUHua, NIUMin-min, CHENDa, LIYue

2014, 30(1): 15-18.

[Abstract](803) [PDF 1149KB](680)

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OBJECTIVE To make a system assessment on TCM drugs treating on children's upper respiratory tract infection with fever， and to analyze the current situation of the clinical studies. METHODS The randomized controlled trial (RCT) of TCM drugs treating children's upper respiratory tract infection with fever published home and broad were searched from 2002 to 2012. According to the data from data base， the literatures' quality were assessed by Jadad standard. Two independent evaluators extract data and assess the quality methodologically. And then the include studies were system assessed by RevMan 4.3 software. RESULTS 112 related literature were retrieved， and 14 of them were qualified including 1989 cases. The Meta analyses shows that the curative effect of TCM drugs treating upper respiratory tract infection with fever are better than western medicines， and the difference was statistically significant [OR=3.66， 95%CI is 2.78~4.82， Z=9.22， P<0.01]. CONCLUSION TCM drugs's curative effect of treating children's upper respiratory tract infection with fever is better than western medicines， however， because the limited quantity and quality of the included literature， the conclusion is not certain yet， and more high qualitied evidence are needed in the future.

Meta-analysis on Efficacy and Life Quality of Advanced Colorectal Cancer with Chinese Medicine Combined with Chemotherapy

LIU Li-rong, ZHU Yan-juan

2014, 30(1): 19-21.

[Abstract](671) [PDF 1135KB](720)

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Eutopic Differential Proteomics Research on Endometriosis Patients with Blood Stasis

HUANG Jin-yan, WEN Yi, WEI Shao-Bin

2014, 30(1): 22-26.

[Abstract](669) [PDF 1452KB](687)

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120 Cases Asthma Children's Chinese Medicine Constitution Classification Study

ZHAOXia, LUHai-yan, SUNYi-qiu

2014, 30(1): 27-29.

[Abstract](841) [PDF 1055KB](950)

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OBJECTIVE To classify the constitutional types and provide the prevention and treatment programs in Chinese medicine for children with asthma. METHODS According to the physical features， daily performance and susceptibility through clinical investigation of 120 cases children with asthma， their constitution types were classified. RESULTS The larger percentage of asthma children present the lung deficiency tending to qi insufficiency (account for13.33%)， yin insufficiency (24.17%)， the spleen deficiency tending to qi insufficiency (20.00%)， the lung-spleen deficiency tending to qi insufficiency (24.17%)， and 22 accompany with phlegm-damp and 17 accompany with phlegm-heat. CONCLUSION The constitutions of asthma in children have individual differences obviously， but they still can be classified by clinic investigation. Most of them are the insufficiency constitution types.

Clinical Research on the Preventive effects of traditional Chinese medicine formula and Interferon α-1b on Hand-foot-and-mouth Disease

YANGHai-ying, WANGMing-yue, ZHANGQuan-fa, XUMan-man, MUChun-sun

2014, 30(1): 30-32.

[Abstract](551) [PDF 1007KB](667)

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OBJECTIVE To investigate the preventive effect of traditional Chinese medicine TCM and interferon (IFN) on children with hand-foot-and-mouth disease (HFMD)， and to provide scientific TCM recipe and integrated traditional prevention guideline for HFMD in Shandong province. MRTHODS During April 2010 and October 2012's hand-foot-and-mouth disease epidemic seasons， 1106 cases children from the disease focus areas were chosen and randomly divided into four groups: prevention group 1 (TCM group); prevention group 2 (TCM +IFN group); prevention group 3 (IFN group) and control group (Blank control group). The course of treatment is 7 days， every case has been followed-up for 4 weeks. The normal and severe cases were counted， adverse reactions were recorded， and each groups' incidence of HFMD and severe cases were compared. RESULTS The incidences of HFMD in the four groups are separately 8.02%， 11.98%， 9.92% and 36.46%. There is significant difference between every preventive group and control group (all P<0.01). There is no statistical difference between the former three preventive groups (P>0 05). The effective index of preventive groups are separately 4.55， 3.06， 3.68; the protective efficacy of the three groups are separately 78.00%， 67.14%， 72.79%. The average incidence of prevention groups is 9.78%， and severe cases accounted for 6.25% (5/80). The incidence of control group is 36.46%， and severe cases accounted for 17.14% (18/105). CONCLUSION The traditional Chinese medicine and interferon can significantly reduce the incidence of hand-foot-and-mouth disease and the incidence of severe cases.

Clinical Research on Guanchang Fang's Inhibit Function on the Growth of Advanced intestinal Cancer and the Expression of Serum VEGF and miRNA31

YUChen, LIUShen-lin, ZOUXi, WUJian

2014, 30(1): 33-36.

[Abstract](647) [PDF 1028KB](666)

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OBJECTIVE To observe intestinal cancer patients' peripheral blood miRNA31 and VEGF expression changes when treated with the Guanchang Fang combined chemotherapy and chemotherapy alone. METHODS A prospective controlled clinical research methods were used and the intestinal cancer patients were randomly divided into 20 cases of experimental group and 20 cases of the control group. The experimental group using Guanchang Fang (made by Herba Agrimoniae， Herba Patriniae， and Herba Solani Nigri) combined with chemotherapy， and the control group treated with chemotherapy (FOLTOX scheme) alone. Guanchang Fang combined chemotherapy's effect on advanced intestinal cancer patients， the efficacy of traditional Chinese medicine syndrome curative effect， clinical curative effect， standards of life quality score， toxicity reflection were observed. Quantitative PCR assay was used to test the post Guanchang Fang intervention serum miRNA31 level changes; analysis of clinical efficacy and miRNA31 correlation was detected by ELISA in human vascular endothelial growth factor (VEGF) expression level changes， the mechanism of Guanchang Fang was explored. RESULTS Guanchang Fang combination chemotherapy's effect on treating advanced intestinal cancer is better than the chemotherapy alone (P<0.05); Comparison of the two groups shows that(P>0.05); The KPS shows combined group's patients life quality are better (P<0.05); the VEGF and miRNA31 of combined group are lower than the chemotherapy group (P<0.01). CONCLUSION Guanchang Fang combined with chemotherapy can control and reduce advanced intestinal cancer serum VEGF levels， and its mechanism may be associated with miRNA31 level down regulation.

Research on Visual Function Protection for Nonarteritic Anterior Ischemia Optic Neuropathy in Nourishing qi， Activating Blood Circulation and Collaterals Method

CHENZi-man, ZHANGMei-fang, LIZhi-ying, JIJian-ping

2014, 30(1): 37-40.

[Abstract](666) [PDF 1021KB](625)

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The effect of acupuncture on pathogenic changes in aortasinus plaque of ApoE-/- mice

TAOWen-jian, ZHANDong-mei, AIBing-wei

2014, 30(1): 41-45.

[Abstract](669) [PDF 2067KB](633)

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OBJECTIVE To observe the change of aorta sinus plaque morphology， the expression of PPARγin atheromatous plaque， the differences of oxidized low density lipoprotein and apolipoprotein B of mice serum among groups after acupuncture intervention in ApoE-/- mice， and to study the mechanism of acupuncture on aorta sinus plaque of ApoE-/- mice. METHODS 34 ApoE-/- male mice at 6 weeks of age were fed with high fat. After 14 weeks， 4 mice were selected randomly and condemned to death to observe morphology change of aorta sinus plaque and to judge whether the atherosclerosis model of construction is succeed. Then the 30 mice were divided randomly into model control group (n=10)， pharmacy control group (n=10) and electro acupuncture group (n=10). Moreover， 4 C57BL/6J male mice were fed ordinary feed as blank control group. Pharmacy group were administered with simvastatin 40mg/kg per day by oral gavage. Acupuncture group were treated by low frequency Electro acupuncture at neiguan， shenmen， housanli and sanyinjiao point. After 11 weeks treatment， blood samples were applied to detect the contents of oxidized low density lipoprotein and apolipoprotein B， besides their arterial aortas were separated and atherosclerosis lesions tissues were observed with HE， the immunohistochemical staining were used to compare the differences of oxidized low density lipoprotein， apolipoprotein B of serum， morphology change of aorta sinus plaque and expression of PPARγin atheromatous plaque among three groups. RESULTS Acupuncture can protect the vascular intima significantly inhibit the development of plaque. The curative effect of acupuncture was better than simvastatin. CONCLUSION Acupuncture can prevent the development of plaque by regulating lipid and energy metabolism preventing oxidation， etc. Acupuncture can play therapeutic effect by reinforcing spleen replenishing qi reducing phlegm and removing blood stasis from TCM perspective.

Tuina's Effect on NT-3/TrkC of Sciatic Nerve Injury Rats

GENG Nan, XIAN Si-tong, WU Jian-cong, LU Meng-qian, YAO Bin-bin, GAO Yu-feng, LI Xiao-qin, YU Yue, YU Tian-yuan

2014, 30(1): 46-49.

[Abstract](617) [PDF 1809KB](621)

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OBJECTIVE To study the Tuina's effect on the sensory function of sciatic nerve injury rats and the NT-3/ TrkC expression. METHODS Using the chucking method to establish the sciatic nerve injury model and the rats were randomly divided into normal group， sham-operated group， model group， model control group and the Tuina group. Imulator was used as intervention to observe the rats be havior change. Immunohistochemical staining was used to observe the expression of each rat dorsal root section in the NT-3 TrkC. RESULTS Compared with the normal group， the model group， model control group rats' PWL value were significantly extended. Compared with model group， the Tuina group's PWL value reduced and no significant difference compared with normal group. Compared with the normal group， model group， model control group and the Tuina group's NT-3 immunohistochemical expression were increased significantly. Compared with model group， control group has significant difference (P<0.05). CONCLUSION TCM Tuina can improve the behavior performance of nerve injury in rats. By promoting the expression of the NT-3 and the receptor TrkC， cell apoptosis were inhibited; the growth of nerve cell body and processes were influenced， which have a positive impact on the damage of nerve repair and the improvement of motor function of sciatic nerve injury in rats.

Effect of Autonomic Discharge of EA Different Acupoints on the Small Intestine Dyskinesia's Rat

KE Hui, CHENG Qian

2014, 30(1): 50-52.

[Abstract](637) [PDF 1087KB](697)

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OBJECTIVE Observe the effect of autonomic discharge when EA different acupoints on the rats with small intestine motility disturbance. METHODS Male Wistar rats were randomly divided into blank control group， model group， electro-acupuncture group. After modeling， electro-acpuncture at Zusanli， Liangqiu， Neiguan and Daling， recording each rat's vagus nerve and splanchnic nerve discharge. RESULTS Autonomic discharge frequency of control group had no significant changes during the observation period. After modeling， model control group vagus nerve discharge frequency was significantly reduced(P<0.01)， splanchnic nerve discharge frequency was significantly higher(P<0.01). The vagues nerve discharge frequency of groups of Zusanli， Neiguan， and Liangqiu， after electro-acupuncture，was significantly higher(P<0.05). The splanchnic nerve discharge frequency of all groups after electro-acupuncture was significantly reduced(P<0.05). CONCLUSION The influence on the small intestine of the autonomic nervous system is one of the reasons why acupuncture is effective.

Effect of Compatibility of Astragalus Plus Zedoary on the Expression of MMP2 and VEGF in Orthotopic Transplantation Tumor Tissue of HO-8910 Ovarian Carcinoma in Situ

YINGang, TANGDe-cai, DAIJian-guo, ZANGWen-hua, WUXiao-yu

2014, 30(1): 53-56.

[Abstract](658) [PDF 1414KB](639)

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OBJECTIVE To observe the effect of Radix Astragali， Rhizoma Zedoariae and antitumor effect and the effect of MMP2 and VEGF expression in tumor tissues of human ovarian cancer HO-8910 orthotopic transplantation tumor. METHODS elect the HO-8910 ovarian carcinoma in situ animal models have been established fluorescent protein transfection(5 grope)，Including model group， positive control group (Shun Bo)， Astragalus group， Zedoary group， Astragalus，zedoary turmeric compatibility group (on the basis of 2∶1). Dynamic measurement of each tumor volume， tumor metastasis， tumor inhibitory effect. The protein expression of MMP2 and VEGF were deteced by immunohistochemical method. RESULTS Astragalus root， zedoary turmeric compatibility group and cisplatin group， tumor volume and tumor weight were lower than model group(P<0.05)， Astragalus， zedoary turmeric compatibility group tumor volume and tumor weight less than cisplatin group，Curcuma group and Astragalus group， Zedoary group of tumor volume and tumor weight compared with the model group（P>0.05）， Astragalus root， zedoary turmeric compatibility group tumor volume and tumor weight less than cisplatin group(P<0.05)， Compared with the model group the level of MMP2 and VEGF protein expression of average weakened Astragalus， zedoary turmeric compatibility group Astragalus group， Zedoary group and cisplatin group in transplanted tumor tissue(P<0.05). CONCLUSION In the experimental period，Astragalus root， zedoary turmeric compatibility which has obvious anti-tumor effect on human ovarian cancer HO-8910(P<0.05)，The action mechanism of the expression may be associated with the downregulation of MMP 2 and VEGF protein.

Comparative Study of “Sini San” and “Erxian Decoction”on Rats with Chronic Psychological Stress and Mammary Hyperplasia Disease (MHD)

SONGTing, TONGZhong, LIZhong-ping, SHENHong-yi

2014, 30(1): 57-60.

[Abstract](699) [PDF 1382KB](669)

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OBJECTIVE To explore effect of Chinese medicine on animal models with mammary hyperplasia disease induced by liver qi stagnation and Chong and Ren pulses disorder in the theory and method of psychosomatic medicine in modern medicine， according to the characteristic of setup of female breast hyperplasia closely related to the theory of pathogenic emotion leading to diseases in TCM. METHODS Combination of chronic stress and mammary hyperplasia was used to make models， during which the behavior changes， animal tissue papilla height after modeling were observed. And serum was detected in radioimmunoassay. RESULTS The modeling method made a big difference on animal behavior. Animal strengh and behavior were improved in each TCM group after treatment and breast tissue hyperplasia was relieved. After treatment with Erxian decoction， Sini powder and the combination， serum indexed were recovered and there were no significant difference among the groups. The ratio in group with the combination was lower E₂/P than that in group with seperated medicine. The hyperplasia was relieved in some degree. CONCLUSION Sini powder and Erxian decoction can improve breast hyperplasia in rats. Chronic stress and breast hyperplasia combined with animal models have induced the effect of breast hyperplasia. Sini powder， Erxian decoction and the combination make no significant differences in serum indicators; in terms of adjusting balance between estrogen and progesterone， the combination is better than the seperated.

Research of Xijiao Dihuang Decoction on expression of autophagy related protein Atg-5 and Beclin-1 in rats with cerebral ischemia

LIUHong-quan, WANGYu, GUOLi-zhong, HUANGHou-cai, ZHONGRong-ling, XIAZhi

2014, 30(1): 61-64.

[Abstract](687) [PDF 1550KB](617)

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OBJECTIVE To investigate the Xijiao Dihuang Decoction on mRNA expression of autophagy related Atg-5、Beclin-1 protein of the rats' brain tissues， which are endotoxin induced persistent middle cerebral artery occlusion (pMCAO) and to observe the anti-body expression of LC-3 through transmission electron microscope. METHODS Dived the rats into different groups after modeling， and then use different dose of Xijiao Dihuang Decoction for intervention. Extract RNA and use PCR to detect the gene expression of Rat-atg-5 and Beclin -1. The antibody expression of stained LC-3 was observed through transmission electron microscope. RESULTS Atg-5， Beclin-1 and mRNA significantly increased in "Stasis heat" syndrome rat model of "pMCAO" and the model group. The expression was significantly reduced Xijiao Dihuang Decoction middle dose group and positive drug group compared with the model group. LC-3 antibody staining granules suggested that the blank group did not have obvious expression. The model group rats， especially in the sixth day， the expression of model group was significantly increased and positive drug group significantly inhibited LC-3 expression. The third and sixth day reached the best point. Low dose group showed no inhibitory effect. The middle dose group had better effect than high dose group and reached the best point on the sixth day. CONCLUSION Xijiao Dihuang Decoction can protect the brain by significantly reducing the expression of Atg-5， Beclin-1 mRNA and inhibiting the expression of LC-3.

Study on the Inhibitory Effect of Yishen Huayu Method in the Mechanism of Fibroblast Proliferation

YINYan-yun, BIANYao-yao, LINGYun, ZHOUChun-xiang

2014, 30(1): 65-67.

[Abstract](692) [PDF 1056KB](599)

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OBJECTIVE To study the effect of Yishen Huayun method on the intervention mechanism of fibroblast proliferation of chronic pelvic inflammatory process. METHODS Different concentrations of YSHY， YS， HY were used in the incubated NIH/3T3 cells and MTT method was used to detect the cell proliferation. And the effect of different prescriptions for bFGF、TGF-β、HOP expression were observed. RESULTS YISHEN Huayun effectively inhibit the expression of fibroblast proliferation and cytokine， and reduce the production of HOP， which is in a dose-dependent manner.Compared with Yishen Huayu method， the inhibitory effect of Yishen method is relatively low; compared with Yinshen Huayu method， Huayu method achieved low effects， but is more useful than Yishen method. CONCLUSION Yishen Huayu method is effective in inhibiting the fibroblast proliferation in treating chronic pelvic inflammatory disease by inhibiting the expression of cytokines related to fibroblast proliferation and the expression of certain collagen in the extracellular matrix. Compared with the Yinshen method and Huayu method， the combined formula can achieve the best effect.

Relationship between photosynthetic characteristics and environmental factors in leaves of Alisma orientalis

ZHOUJuan-juan, GUWei, CHENJing-ying, WUQi-nan, CHAOJian-guo

2014, 30(1): 68-71.

[Abstract](717) [PDF 1492KB](630)

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OBJECTIVE To study the relationship between photosynthetic characteristics and environmental factors in leaves of Alisma orientalis. METHODS The daily variation of net photosynthetic rate， environmental factors and photo-response in leaves of A. orientalis were measured by LI-6400 Portable Photosynthesis Apparatus. RESULTS The net photosynthetic rate (Pn) and stomatal conductance (Gs) showed a diurnal variation of bimodal curve. The transpiration rate (Tr)， intercellular CO2 concentration (Ci) and stomatal limitation (Ls) presented a single peak diurnal variation. The water use efficiency (WUE) was a single valley type of diurnal variation. Correlation analysis indicated that there were positive correlations between Pn of A. orientalis and photosynthetic active radiation (PAR)， Gs， Tr or WUE， but negative correlations between Pn and relative air humidity (RH)， Ci or Ls. CONCLUSION A. orientalis is the type of stomatal limitation and sun plant. The net photosynthetic rate of A. orientalis leaves has an obvious "midday depression" phenomenon. The results suggest that stomatal conductance， water use efficiency， photosynthetic active radiation and transpiration rate are the main environmental factors influencing the A. orientalis photosynthetic rate. This study could offer certain theoretical reference for A. orientalis rational cultivation， as well as provide a scientific basis for improving the quality and production of medicinal materials.

Rapid Determination of Geniposide Content During Gardenia Countercurrent Extract Process Through near Infrared Spectroscopy

YANGPei-pei, ZHOUHan-yu, CONGXiao-dong, CAIBao-chang, QIANJian-hua, FANGXiao-min, PENGMei-xian

2014, 30(1): 72-75.

[Abstract](600) [PDF 1199KB](802)

Abstract:
OBJECTIVE To rapidly determine geniposide content during gardenia countercurrent extract process through near infrared spectroscopy(NIR). METHODS NIR combined with partial least squares (PLS) was established to rapidly determine geniposide content with HPLC as the reference. RESULTS The geniposide correlation coefficient (R) and root-mean-square error of calibration (RMSEC) were respectively 0.997 and 0.113. After an external validation， the root-mean-square error of prodiction (RMSEP) was 0.132 and the correlation coefficient of predicted and true values reached 0.996. CONCLUSION NIR can be used as a kind of accurate， rapid and nondestructive detection method applied in studing changes of active ingredients content during traditional Chinese medicine countercurrent extraction process.

Content Determination of Major Nucleosides in Supernatant of Mactra veneriformis， Ruditapes philippinarum， and Meretrix meretrix Linnaeus by Ethanol Subsiding Method

LU Jie-lin, CHAI Yao, JIA Meng-jiao, LIU Rui, WANG Ling-chong, WANG Xin-zhi, WU Hao

2014, 30(1): 76-78.

[Abstract](756) [PDF 1075KB](776)

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OBJECTIVE To establish a method for determining uridine， xanthine， thymine， hypoxanthine， inosine， guanosine， 2-deoxyguanosine， 2-deoxy inosine， thymidine， adenosine in the supernatant of Mactra veneriformis， Ruditapes philippinarum， and Meretrix meretrix Linnaeus by ethanol subsiding method. METHODS The xBridge C18 analytical column(4.6 mm×250 mm，5 μm) was used and the mobile phases were consisted of water and methanol with gradient elution. The flow rate was 0.5 mL/min， column temperature was at 25 ℃， and the wave length was 254 nm. RESULTS The supernatant of Mactra veneriformis， Ruditapes philippinarum and Meretrix meretrix Linnaeus by ethanol subsiding method contains uridine， xanthine， thymine， hypoxanthine， inosine， guanosine， 2-deoxyguanosine， 2-deoxy inosine， thymidine， and adenosine. CONCLUSION This method is easy to practice and accurate and can be used for determining the main nucleoside of supernatant by the ethanol subsiding method for clams.

Determination of Chlorogenic Acid， Peoniflorin， Schisandrin， and Schizandrin B in Compound Wu Ren Chun Soft Capsule by HPLC

ZHOUShan, ZHOUXiao-dong, DINGQing-long, DILiu-qing, KANGAn, ZHOUWei

2014, 30(1): 79-82.

[Abstract](637) [PDF 1362KB](839)

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Simultaneous Determination of Six Components in Compound Wuwei Buccal Tablets by HPLC

SHAN Xin, LU Tu-lin, MAO Chun-qin, JI De, LANG Yong-yin, HUANG Shuang

2014, 30(1): 82-84.

[Abstract](722) [PDF 1050KB](667)

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OBJECTIVE To establish a method to simultaneously determine puerarin， liquiritin， ammonium glycyrrhetate， schizandrol， deoxyschisandrin， and schizandrin B in compound wuwei tablet by HPLC. METHODS The elusion was performed on Kromasil C18(250 mm×4.6 mm， 5 μm） column. The mobile phase was composed of acetonitrile and 0.05% H3PO4 with gradient elution. The detection wave length was 250 nm， the flow rate was 1.0 mL/min and the column temperature was maintained at 30 ℃. RESULTS Puerarin， liquiritin， ammonium glycyrrhetate， schizandrol， deoxyschisandrin， schizandrin B are all linear in the range of 0.031 0~0.154 9，0.013 7~0.068 5，0.042 4~0.211 8，0.017 4~0.087 1，0.001 8~0.009 1，0.013 6~0.068 1 mg/mL respectively. Their average recoveries were 98.6%，100.4%，98.3%，98.3%，96.8%，99.4% and RSD of the above six components were 2.6%，2.8%，1.2%，2.3%，0.9%，2.5%. CONCLUSION The method is easy to perform and accurate and practical so that it can be used to determine the six components in the compound wuwei buccal tablets.

Qiju Dihuang Decoction in Modification Treating 40 Cases of Combination of Perimenopausal Hypertension and Anxiety

CHEN Lin-rong, PAN Hong-yi, LI Chuang-peng, SUN Jing, PENG Min, TAN Yong-wen

2014, 30(1): 85-87.

[Abstract](688) [PDF 1007KB](747)

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Simultaneous Determination of three components in Compound Hu-huang Spray by UPLC

DAIHong-feng, YINQing-feng, XIETong

2014, 30(1): 88-89.

[Abstract](595) [PDF 1035KB](701)

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OBJECTIVE To develop a quantitative method simultaneously to determinate polydatin and baicalin in compound Hu-huang Spray by UPLC. METHODS The seperation was performed on a column Acquity UPLC BEH C18(100 mm×2.1 mm，1.7 μm). The mobile phase was composed of acetonitrile (A) and 0.05% H3PO4(B) with gradient elution. The flow rate was 0.4 mL /min with the wave length at 280 nm. The temperature was 35℃. RESULTS For polydatin， the calibration curve was linear (r=0.9992) in the range of 0.59~59.0 mg/L， the average recovery was 100.6% (RSD=1.56%). For baicalin， the calibration curve was linear (r=0.9999) in the range of 1.128～112.8 mg/L， the average recovery was 101.5% (RSD=1.68%). For imperatorin， the calibration curve was linear (r=0.999 9) in the range of 0.135～13.5 mg/L， the average recovery was 100.2% (RSD=3.03%). CONCLUSION The method was sensitive， accurate and can be used in the quality control of compound Hu-huang Spray.

Simultaneous Determination of Loganin and Morroniside in Total Glucosides from Cornus officinalis by HPLC

LI Wei, XU Hui-qin, TAO Yu-han, NONG Wei-hu, WU Jia-lei

2014, 30(1): 90-92.

[Abstract](615) [PDF 1094KB](740)

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OBJECTIVE To set up a method to simultaneously determine loganin and morroniside in total iridoid glycosides of cornus officinalis by HPLC， and to provide the basis for the quality evaluation. METHODS The analysis was performed on a Lichrosphere C₁₈ column and the mobile phase was composed of acetonitrile-water with a linear gradient elution. The flow rate was 1.0 mL/min， the temperature of column was 30 ℃， and the UV detection wave length was set at 240 nm. RESULTS Loganin and morroniside were linear in the ranges of 0.088~0.528 μg(r=0.999 8) and 0.088~0.352 μg(r=0.999 5)， respectively. The average recoveries were 98.7%（RSD=1.3%）and 98.6%（RSD=0.8%）. CONCLUSION The method of HPLC is accurate， easy to practice and reproducible， which can be used to control the quality of total iridoid glycosides of cornus officinalis.

Experimental Research Progress of Traditional Chinese Medicine Preventing and Treating Peritoneal Adhesion

ZENGLi, YANShuai, LIWen-lin

2014, 30(1): 93-96.

[Abstract](742) [PDF 1055KB](696)

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Discussion on the Application and Problem of Traditional Chinese Medicine Water Extraction Concentration Technique Using Membrane Distillation

PAN Lin-mei, SHI fei-yan, GUO Li-wei

2014, 30(1): 97-100.

[Abstract](669) [PDF 1046KB](924)

Abstract:
Combined with the basic concepts and the characteristics of membrane distillation process， the affecting factors of membrane distillation performances and comprehensive overview in other areas. This article gives the corresponding countermeasures to existing problems in the membrane distillation process against the complexity of traditional Chinese medicine(TCM) extract . Suitable membrane distillation technology has good application prospects in the enrichment process of TCM extract.

2014 Vol. 30, No. 1

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