南京中医药大学学报

Reflection of Improving the Effect of Traditional Chinese Medicine Differentation and Treatment on Treating Chronic Viral Hepatitis Differentially

ZHOU Zhong-ying

2013, 29(6): 501-502.

[Abstract](621) [PDF 304KB](686)

Abstract:
Viral hepatitis with high incidence， wide prevalence and slow and hidden onset recurs easily， heals procrastinately and even develops into cirrhosis of the liver and liver cancer， thus belonging to resistant disease. Although traditional Chinese and western medicine have made great progress in the treatment， it is difficult to obtain satisfactory clinical effects due to many problems existed. From the concept of traditional Chinese medicine itself， how to improve the effects of TCM on treating chronic viral hepatitis differentially should be the key issue. Six aspects of syndrome differentiation， namely damp-heat retention， vessel stasis due to long-term illness， hidden toxin accumulation， spleen deficiency caused by stagnation of liver qi， zang-organ diseases affecting fu-organ， lingering pathogen due to vital qi deficiency should be paid more attention to. Treatment strategies should stress four aspects， namely treatment according to syndrome differentiation， reinforcement of vital qi to inhibit pathogen toxin， differences aside on common ground and main points of formulates.

Discussion on Diversity Factors of Blood-activating and Stasis-resolving Medicine Treating Cancer Metastasis

TANG De-cai, ZANG Wen-hua, ZHU Ye-qiong, LI Bing-bing

2013, 29(6): 503-506.

[Abstract](676) [PDF 429KB](869)

Abstract:
Blood-activating and stasis-resolving medicine is a common drug to treat tumors. Most scholars believe that blood-activating and stasis-resolving medicine， especially expelling blood circulation drugs， is effective to treat malignant tumor， while some scholars believe that the application of such drugs will promote tumor metastasis and diffusion. These drugs may have a positive or negative effect on cancer invasion and metastasis. It makes them more controversial. Through pharmacological experiment and clinical observation， blood-activating and stasis-resolving medicine conforms to differential diagnosis and treatment of TCM in treating malignant tumor. These drugs are essential in the prevention and treatment of cancer. To make blood-activating and stasis-resolving medicine produce anti-tumor invasion and metastasis， the key factors are discerning the nature and location of tumor， choosing the right breed， taking appropriate compatibility and selecting appropriate time according to TCM.

Discussion on Treating Scleroderma from the Lung

CHEN Jian-mei, GUO Feng, QIAN Xian

2013, 29(6): 507-509.

[Abstract](602) [PDF 363KB](781)

Abstract:
Treating scleroderma from the lung is put forward based on the theory of "lung governing fur and hair". This paper discusses the theory connotation， modern understanding of lung and fur and hair and correlation between lung and scleroderma， summarizes the pathogenesis and syndrome characteristics， providing a new idea for treating scleroderma from the lung.

Comparative Study on Classification and Coding of ATC and Chinese Traditional Patent Medicine

CHENHong-dou, JIANGJin-guang, ZHUXiu-yin

2013, 29(6): 510-513.

[Abstract](884) [PDF 342KB](1415)

Abstract:
OBJECTIVE To compare the classification and coding between Anatomical-Therapeutic-Chemical (ATC) and Chinese traditional patent medicine to provide some advice and reference for the standardization， information and internationalization of TCPM. METHODS ATC classification and coding was compared with those of Classification and Coding of Chinese Materia Medica， China Pharmacy Pharmainfo Net (CPPN) and Japanese Kampo medicine through descriptive analysis method in classification levels， classification basis and coding approaches to attain some experience and effect for classification and coding of Chinese patent medication. RESULTS The classification methods of Chinese traditional patent medicine according to diseases and syndromes， effect and comprehensive condition are drawing lessons from ATC model， more strict logic， partially achieved the connection with ATC. CONCLUSION Classification of Chinese traditional patent medicine can take example from that of ATC and gradually gets in line with international classification and coding.

Research on Compatibility Laws of Formulae for Lung Distension Based on Regulation Rules and Other Mining Methods

CHENFei, FANXin-sheng, WANGChong-jun, BIANYa-li, JIANGChen-xue, LIYun

2013, 29(6): 514-516.

[Abstract](597) [PDF 388KB](705)

Abstract:
OBJECTIVE To investigate core medicine and their relationship in formulas for lung distension to explore the compatibility law. METHODS After researching all prescription literatures， 194 formulas for lung distension were selected and analyzed by correlation analysis and hierarchical clustering analysis. RESULTS The results of correlation analysis showed medicine whose support was more than 20% were glycyrrhiza， almond， ephedra， pinellia， poria， tangerine peel and schisandra chinensis， and as well as some medicine groups， including almond-ephedra-glycyrrhiza，ephedra-glycyrrhiza-pinellia and ephedra-schisandra chinensis-asarum. The results of hierarchical clustering analysis showed medicine warming the lung to resolving phlegm including ephedra， glycyrrhiza， pinellia， rhizoma zingiberis， cassia twig，schisandra chinensis and asarum. Medicines clearing the lung to remove heat include gypsum fibrosum， almond， ephedra and glycyrrhiza， medicine regulating qi and dissipating phlegm include tangerine peel， citrus aurtantium， and balloonflower. Medicines activating blood to resolve blood stasis and phlegm include angelica， peach kerne， ligusticum chuanxiong， succus bambusae and medicines fortifying the spleen and warming the kidney include aconite， atractylodes and poria. CONCLUSION The lung distension prescriptions may ventilate the lung to remove palegm， warm the lung to reduce fluid， clear the lung to remove heat， activate blood to resolve blood， as well as warm the kidney and fortify the spleen. This study is useful for clinical application in treating lung distension-related diseases based on syndrome differentiation.

The Application of Corresponding Analysis in the Study of Symptoms and Drugs' Relationship in Hypertension

YANGJian-xin, YUANYe, SHENChun-ti, CHENBing-wei, CHENQi-guang, LUYan, LIJie

2013, 29(6): 517-519.

[Abstract](613) [PDF 361KB](618)

Abstract:
OBJECTIVE To investigate the relationship between clinical symptoms and the corresponding drugs of hypertension. METHODS The relationships of symptoms and drugs in 1493 hypertension cases were analyzed correspondingly. RESULTS The relationships of symptoms and drugs in hypertension cases were as follows， the corresponding drugs treating syndrome of hyperactivity of yang due to yin deficiency mainly were: Herba Taxilli， Fructus Corni， Rhizoma Alismatis， Cortex Eucommiae， Rhizoma Dioscoreae; the corresponding drugs treating syndrome of static blood blocking collaterals mainly were Radix Puerariae Lobatae， Flos Cartham，Rhizoma Chuanxiong， Semen Persicae， Radix et Rhizoma Salviae Miltiorrhizae， Radix Paeoniae Rubr， Radix et Rhizoma Glycyrrhizae; the corresponding drugs treating phlegm dampness and blood stasis mainly were Caulis Bambusae in Taenia， Rhizoma Pinelliae， Pericarpium Citri Reticulatae， Rhizoma Acori Tatarinowii， Rhizoma Atractylodis Macrocephalae， Poria; the corresponding drugs treating qi and blood deficiency syndrome mainly were Radix Angelicae Sinensis，Radix Astragali，Radix Codonopsis，Radix Paeoniae Alba; the corresponding drugs treating hyperactivity of liver fire syndrome mainly were Radix Rehmanniae，Radix et Rhizoma Gentianae，Spica Prunellae，Fructus Gardeniae，Radix Scutellariae，Flos Chrysanthemi. CONCLUTION This study analyzed the relationship of symptoms and drugs in hypertension correspondingly and offered clinical bases for hypertension's Chinese medicine differentiation.

Analysis of Clinical Symptoms and Signs Distribution in 1266 HIV/AIDS Cases

HUIGao-meng, ZHENGZhi-pan, MENGPeng-fei, MAXiu-xia, TANGYin-yin, SONGXi-yuan, XULi-ran

2013, 29(6): 520-523.

[Abstract](612) [PDF 339KB](875)

Abstract:
OBJECTIVE To analyze clinical symptoms and signs distribution in 1 266 HIV/AIDS cases for laying the foundation of further clinical studies. METHODS The information on 1 266 HIV/AIDS cases selected from October 2004 to October 2009 was processed and made a statistical analysis. RESULTS Among the 1 266 cases， male took up 48.3% while female 51.7% with the ratio of 0.93∶1. Adults from 31 up to 40 years old accounted for 45.9% as the most. The majority were infected through blood transfusion， occupying 87.7%， manifesting fatigue， pale tongue and white coating and thin pulse as the main symptoms. CONCLUSION HIV/AIDS patients are mainly infected with blood transfusion. HIV/AIDS patients manifest with deficient syndromes and signs with qi deficiency as the basis and spleen as the pivot.

Effect of Yunpiqiangsheng Fang on Improving Life Quality of Peritoneal Dialysis Patients

JIANGYan, SHENGMei-xiao, SONGHuan, YUANXiao-ling, YINLing

2013, 29(6): 524-528.

[Abstract](646) [PDF 414KB](606)

Abstract:
OBJECTIVE To observe the effect of Yunpiqiangsheng Fang on improving quality of life (QOL) of peritoneal dialysis (PD) patients. METHODS 55 patients were divided into treatment group (26) and control group (29). Control group received western medicine and conventional PD treatment apart from which treatment group was added Yunpiqiangsheng Fang as well for three months. After the treatment， changes in KDQOL-SFTM1.3 scale score， nutrition indicators (Hb， Alb， pre-A， TSF， MAC， MAMC， MQSGA)， metabolic parameters (TC， TG， Ca²⁺， P³⁺， PTH) and dialysis-related indicators (BUN， Scr， KT / V， Ccr) were observed. RESULTS ① Treatment group can increase KDQOL-SFTM1.3 scale score with total effective rate 83.3 %， superior to control group (P<0.01) especially in improving the symptoms， health condition and sleep， relieving the impact of kidney disease for life， and enhancing strengh(P<0.05). ② After treatment， MQSGA in treatment group significantly decreased (P<0.01) while Hb， Alb， pre-A， TSF increased (P<0.05) compared with that before treatment. The changes of MQSGA， Hb， pre-A， TSF are better than that of control group (P<0.05). ③ There is no statistically significant difference in TC， TG， Ca²⁺， P³⁺， PTH among the groups (P>0.05). ④ BUN in treatment group is decreased (P<0.05) whose change is larger than that of control group (P<0.05). There is no statistically significant difference in Scr，KT/V，Ccr among the groups (P>0.05). CONCLUSION Yunpiqiangsheng Fang can help improve PD patients' QOL， which plays an important role in increasing appetite， enhancing digestion and absorption， regulating overall function of organs， thereby improving patients' nutrition.

Shugan Tiaoyinyang Fang in Ameliorating Symptoms Caused by Tamoxifen after Breast Cancer Surgery and Improving the Quality of Life: a RCT

BIANWei-he, LILin, ZHANGXiao-qing, YANGJing, YINGYu, LEYin-zi, CHENLing, CAODong-yang

2013, 29(6): 529-531.

[Abstract](754) [PDF 353KB](686)

Abstract:
OBJECTIVE To evaluate the clinical effects of Shugan Tiaoyinyang Fang on treatment of symptoms caused by oral Tamoxifen. METHODS 80 patients with breast cancer who had been treated with Tamoxifen for more than one year and had obvious endocrine disorders were randomly and equally divided into two groups， namely the treatment group of 40 cases orally taking Shugan Tiaoyinyang Fang and Tamoxifen twice a day for 60 days and the control group of 40 cases only orally taking Tamoxifen. The amended kuppermann scores were adopted to evaluate the improvement of the symptoms， KPS was adopted to evaluate patients' quality of life and laboratory tests were used to evaluate drug safety.RESULTS The Kuppermann scores of the treatment group degreased significantly after 15-day treatment to the end of the study (P<0.05~0.01)， while only some of the symptoms of patients in the control group were improved， such as vertigo， joint pain， headache， palpitation (P<0.05~0.01). The Kuppermann scores of the treatment group degreased significantly with statistical differences after treatment compared with those before treatment (P<0.05)， while those of the control group were not changed obviously (P>0.05). The KPS scores of the treatment group improved significantly， compared with the control group (P<0.05). The laboratory test results showed that the blood test and hepatic and renal function test were normal for all the patients and there was a significant decrease of the triglyceride level in the treatment group (P<0.01) . CONCLUSION Shugan Tiaoyinyang Fang can effectively ameliorate the symptoms caused by Tamoxifen improve patients' life quality reduce the blood lipid level and lessen the side effects of Tamoxifen in clinic.

Influence of Scalp Acupuncture Treatment for Cognitive Function and Brainstem Auditory Evoked Potential on Children with Cerebral Palsy

TANG Jian, ZHANG Yue, ZHU Min, FU Da-lin, LI Hong-ying, ZHAO Xiao-ke, DU Sen-jie, ZHANG Ling, ZHANG Li

2013, 29(6): 532-534.

[Abstract](595) [PDF 346KB](608)

Abstract:
OBJECTIVE To investigate the influence of scalp acupuncture treatment on cognitive dysfunction and brainstem auditory evoked potential on children with cerebral palsy. METHODS 40 cerebral palsy patients with cognitive dysfunction were recruited and randomly divided into treatment group (20 cases using scalp acupuncture treatment and comprehensive rehabilitation training) and control group (20 cases only using comprehensive rehabilitation training). After a course of therapy， Gesell developmental scales and brainstem auditory evoked potential of both groups were statistically analyzed. RESULTS Gesell developmental scales was in a statistically significant difference compared with before in both groups (P<0.01， P<0.05). The wave PL， IPL andⅤwave response threshold were improved significantly. There was significant difference between two groups after treatment (P<0.05).CONCLUSION Scalp acupuncture treatment is more effective than rehabilitation training alone in improving cognitive dysfunction of cerebral palsy， and it can effectively improve the abnormal BAEP.

Clinical Observation of 60 Cases of Multiple Verruca Plantaris Treated by Jiedu Quyou Fang through Local Hyperthermic Soaking

CHEN Li, YOU Shan-shan, XU Ping

2013, 29(6): 535-537.

[Abstract](636) [PDF 346KB](613)

Abstract:
OBJECTIVE To discuss the clinical efficiency of Jiedu Quyou Fang in the treatment of multiple verruca plantaris. METHODS 60 cases were collected from Department of Dermatology in Jiangsu Provincial Hospital of Traditional Chinese Medicine and were randomly divided into the treatment group (30 cases) and the control group (30 cases).The treatment group took the powder of Jiedu Quyou Fang by local hyperthermic soaking，while the control group had soaking and tretinoin cream. Patients in each group received treatment every night for 4 weeks as one course. After 1 to 3 courses' continuous treatment， the clinical effects and changes were evaluated. RESULTS 7 cases fully recovered， 9 cases had obvious effect， 10 cases improved， 4 cases had no effect in the treatment group， and the total effective rate was 86.67%. In the control group，no case fully recovered， 2 cases had obvious effect， 9 cases became better， 19 cases had no effect and the total effective rate was 36.67%. The curative effect of the treatment group was better than that of the control group. With statistical analysis， the integral index and overall efficacy difference of both groups had statistical significance (P<0.05). CONCLUSION Jiedu Quyou Fang， which is simple and cheap， can effectively treat the multiple plantar warts without trauma， worth of clinical application.

Experimental Study on Antibacterial and Antiviral Actions of Xiao'er Jinning Oral Liquid

SHIYan, XUHui-qin, YUJing-hua

2013, 29(6): 538-543.

[Abstract](589) [PDF 1203KB](577)

Abstract:
OBJECTIVE To observe effect of Xiao'er Jinning Oral Liquid on bacterium and virus in vivo and vitro. METHODS The antibacterial effect in vitro of Xiao'er Jinning Oral Liquid on common 12 kinds of pathogenic bacteria in clinic was observed through test tube methods while antibacterial affected in vivo by infecting mice with Staphylococcus aureus and Streptococcus pneumoniae. The antiviral effect in vitro was observed through cytopenic method induced by influenza A and influenza B while antiviral effect in vivo through mouse models with respiratory infection by influenza virus and Sendai virus. RESULTS Xiao'er Jinning Oral Liquid had an inhibitory effect on the 9 kinds of bacteria， influenza A， influenza B in different degrees， especially more for Staphylococcus aureus and influenza A， reduced mortality rates of mice infected by Staphylococcus aureus，influenza virus and Sendai virus with varying degrees， prolonged the survival time， decreased lung index of infected mice and diminished pulmonary pathological lesion. CONCLUSION Xiao'er Jinning Oral Liquid has some antibacterial and antiviral effect.

Synthesis of Naringenin Complexes with Cu and Zn and the Inhibiting Effects on Experimental Choroidal Neovascularization in Rats

XUXin-rong, YUHai-tao, HANGLi, SHAOYan, DINGShu-hua, YANGXue-wen

2013, 29(6): 544-547.

[Abstract](644) [PDF 463KB](618)

Abstract:
OBJECTIVE To compare the inhibiting effects of naringenin complexes with Cu and Zn with free ligand on laser-induced choroidal neovascularization (CNV) in rats. METHODS Naringenin complexes with Cu and Zn have been synthesized and characterized on the basis of elemental analyses， i.r. spectra， u.v. spectra. Male Brown Norway rats were anesthetized to receive krypton laser to induce CNV. 20 model rats were divided randomly into control， naringenin complexes with Cu and Zn groups， 5 rats in each group. Naringenin and its complexes (20mg/kg/d )were given once-daily through intraperitoneal (i.p.) injection after laser treatment for 4 weeks. The sizes of CNV lesions were measured by choroidal flatmounts with fluorescein isothiocyanate dextran for four weeks. RESULTS The animals treated with naringenin complexes with Cu and Zn showed significant decrease in their average CNV sizes (20.90±1.47)×10³μm²， (13.24±1.33)×10³μm²， (13.0±1.28)×10³μm²， compared with the vehicle-treated animals (34.56±1.67)×10³μm²， P<0.05 and <0.01 respectively. Furthermore， naringenin complexes with Cu and Zn reduce the CNV size even more than naringenin (P<0.05 ) . CONCLUSION These results indicate that the biological activity of naringenin can be enhanced by the formation of complexes with Cu and Zn， and complexes of naringenin have stronger inhibition on CNV in the rat model than that of free ligand .

Impact of Jianpi Qinghua Fang on Renal Function， Proteinuria and Kidney Col-IV Expression in Rats with Renal Fibrosis

CHEN Xian, HE Li-qun

2013, 29(6): 548-552.

[Abstract](632) [PDF 598KB](610)

Abstract:
OBJECTIVE To investigate the impact of Jianpi Qinghua Fang on renal function， proteinuria and kidney Col-IV expression in focal segmental glomerulosclerosis rats induced by nephrectomy combined with adriamycin. METHODS The model of focal segmental glomerulosclerosis was prepared by the unilateral nephrectomy and the two injection of adriamycin in caudal vein of rat at a dose of 3 mg/kg. The gastric infusion with Jianpi Qinghua Fang， the disassembled prescription of Jianpi Qinghua Fang and Niaoduqing Capsule were administered separately for 8 weeks. The renal function and 24 h proteinuria were determined. The expression of Kidney Col-IV was determined by Real-Time Q-PCR. RESULTS Compared with control group and sham operation group， levels of SCr， BUN， 24 h proteinuria and kidney Col-IV expression in animals of model group were significantly higher (P<0.01). Compared with model group， in the group with Jianpi Qinghua Fang， levels of SCr， BUN， 24 h proteinuria and kidney Col-IV expression decreased significantly (P<0.01). Compared with model group， in the group with Qingre Huashi prescription， level of BUN decreased (P<0.05)， while levels of SCr and kidney Col-IV expression decreased significantly (P<0.01). Compared with model group， in the group with Niaoduqing Capsule， levels of SCr and 24 h proteinuria decreased (P<0.05) while levels of BUN and kidney Col-IV expression decreased significantly (P<0.01). Compared with model group， in the group with Jianpi Yiqi prescription， levels of SCr， BUN， 24 h proteinuria and kidney Col-IV expression unchanged (P>0.05). CONCLUSION Jianpi Qinghua Decoction could inhibit the accumulation of extracellular matrix and the proliferation of glomerular mesangium by lowering the expression of Col-IV in kidney tissue.Therefore，the renal fibrosis was relieved by Jianpi Qinghua Decoction，and the residual renal function was protected.The disassembled prescription Qingre Huashi prescription has the close curative effect in improving the renal function and renal fibrosis，prompting herbs of clearing heat and removing dampness is the key of Jianpi Qinghua Decoction against renal fibrosis.

Protective Effect and Mechanism of Liuwei Dihuang Combined with Huanglian Jiedu Decoction on Diabetic Nephropathy Rats

SHEN Lan, YU Li-qiang, XIONG Pei-hua

2013, 29(6): 553-557.

[Abstract](658) [PDF 824KB](638)

Abstract:
OBJECTIVE To observe the effect of Liuwei Dihuang combined with Huanglian Jiedu Decoction on the expression of serum tumor necrosis factor-α (TNF-α)， interleukin-17 (IL-17)， vascular endothelial growth factor (VEGF) and C reactive protein (CRP) in diabetic nephropathy rats， and to investigate the protective effect on renal function and its mechanism. METHODS 10 SD rats were randomly picked to form normal control group and the left 50 rats were induced into diabetic nephropathy model by intraperitoneal injection of streptozotocin (35 mg/kg) and fed with high calorie forage， then randomly divided into 3 groups: model group， Liuwei Dihuang combined with Huanglian Jiedu Decoction group， and rosiglitazone group. The effects of Liuwei Dihuang combined with Huanglian Jiedu Decoction on serum levels of glucose， insulin， triglyceride， total cholesterol， creatinine， blood urea nitrogen (BUN)， CRP and urinary protein were observed. The expression of the serum TNF-α， IL-17 and VEGF were examined and the kidneys were determined to observe the renal pathological changes. RESULTS Compared with the model group， levels of serum glucose， insulin， triglyceride， total cholesterol， creatinine， BUN， CRP and urinary protein in the Liuwei Dihuang combined with Huanglian Jiedu Decoction group significantly decreased; the serum TNF-α， IL-17 and VEGF levels also decreased， while the kidney organization pathology damage was reduced obviously. CONCLUSION The treatment for diabetic nephropathy rats with Liuwei Dihuang combined with Huanglian Jiedu Decoction could lead to the decrement of urinary protein， improvement of renal function and reduction of renal pathology damage， and the mechanism might related to its action in inhibition extra expression of TNF-α， IL-17， VEGF， CRP in diabetic nephropathy rats.

Biosynthesis of Puerarin Glycoside and Proliferation Inhibition Effect on Breast Cancer Cell

WU Xue-ming, XU Ting-ting, PAN Yang, LIU Chun-mei

2013, 29(6): 558-560.

[Abstract](626) [PDF 405KB](594)

Abstract:
OBJECTIVE To study the optimal transforming conditions for biosynthesis of puerarin glycoside by Acinetobacter johnsonii G2， and the proliferation inhibition effect of puerarin glycoside on human breast cancer cell line MDA-MB-231. METHODS Effect of temperature， pH and sucrose concentration on transformation rate in biosynthesis system were studied. Besides， effects of different concentrations of puerarin and puerarin glycoside on the proliferation of MDA-MB-231 cells through methyl thiazolyl tetrazolium trace enzyme reaction colorimetry (MTT assay) were investigated. RESULTS The best transformation condition of puerarin glycoside was 30 ℃， pH 7.38 and sucrose concentration of 60 g/L. In this condition， the transformation rate was 85% after 24 h. MTT assays showed that different concentrations of puerarin and puerarin glycoside all could inhibit proliferation of MDA-MB-231 cells， and the inhibition effect strengthened with the concentration increased; among which the inhibition rate of 200μmol/L puerarin and puerarin glycoside on MDA-MB-231 cells were 61.91% and 59.42% respectively after 48 h. CONCLUSION Acinetobacter johnsonii G2 can effectively catalyze synthesis of puerarin to puerarin glycoside; both puerarin and puerarin glycoside can significantly inhibit the proliferation of human breast cancer cell line MDA-MB-231.

Effect of Sinopodophyllum Hexundrum on Breast Cancer Tumors in Mice

YE Yao-hui, LIU-bo, MA Yue-xing, YING Ya-bin, ZHANG En-hui, WANG Ting, ZHANG Wen-xue

2013, 29(6): 561-563.

[Abstract](638) [PDF 773KB](729)

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OBJECTIVE To study the preventing and treating effect of Sinopodophyllum Hexundrum on breast cancer tumors in mice model and its mechanism. METHODS The BALB/c mice were injected with 4T1 cells combined with immune suppressor. According to weight， all mice were randomly divided into 6 groups， normal group， model group， Tamoxifen group (4.1 mg/kg) and high， medium and low Sinopodophyllum Hexundrum dose group（1.2， 0.6， 0.3 g/kg）， and administrated drugs intragastrically. After 25 days， quality of tumors and changes of visceral index， WBC count and histomorphology were detected. RESULTS Sinopodophyllum Hexundrum significantly reduced tumor weight and decreased tumor， spleen and thymus index， and WBC count. Tissue section showed promotion of tumor cell differentiation. CONCLUSION Tibetan medicine Sinopodophyllum hexandrum has significant preventing and treating effect on breast cancer， and the mechanism may be related to immunosuppression.

Effects of Stimulating Food on Allergic Contact Dermatitis in BALB/c mice

HU Yang, LI Ting, CHEN Xiang-ming, ZHANG Hai-qing, YU Ai-hua, WANG Guo-jiang

2013, 29(6): 564-566.

[Abstract](651) [PDF 337KB](720)

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OBJECTIVE To study the effects of diet fawu on allergic contact dermatitis (ACD) in mice models and to explore the possible mechanism. METHODS Mice models of ACD was established and the effects of mutton， bamboo shoots， yellow wine， green pepper， sea shrimp on inflammatory reaction and related cytokines was observed respectively at 48 h after elicitation. RESULTS Compared with the control group， mutton， yellow wine， green pepper groups can significantly increase the ear swelling and the number of infiltrating inflammator cells in mice models（P<0.05）， bamboo shoots， sea shrimp groups showed no significant difference (P>0.05). The ear weight changes of each group except mutton showed no significant difference compared with the control group (P>0.05); mutton， yellow wine， green pepper groups can markedly increase expression level of IFN- γ， IL-17， IL-2 in peripheral blood (P<0.05)， bamboo shoots group decreased (P<0.05)， no significant effect of shrimp group (P>0.05). CONCLUSION Mutton， green peppers， yellow wine can aggravate ACD in mice possibly by up-regulation the expressions of IFN-γ， IL-17， IL-2. Bamboo shoots and sea shrimp have no effect on mice ACD.

Research on Removing Sudden Pyrogen Contamination in Baicalin Injection

ZHI Xing-lei, LI Cun-yu, LI Hong-yang, PENG Guo-ping

2013, 29(6): 567-571.

[Abstract](630) [PDF 452KB](606)

Abstract:
OBJECTIVE To optimize the pyrogen removing process of sudden contamination in injections containing baicalin. METHODS The bacterial endotoxin (BE) and effective composition contents in baicalin solution were detected by kinetic turbidity method and HPLC before and after using different concentrations of activated carbon and different molecular weight cut off (MWCO) ultrafiltration membranes to calculate the removal rate of BE and baicalin recovery rate， respectively. RESULTS Under the condition of pyrogen sudden pollution， BE removal rate in baicalin solution were 80.2%， 91.7% and 95.6% when using 0.10%， 0.50% and 1.00% activated carbon， and recovery rate of baicalin were 90.8%， 73.1% and 51.3%. BE removal rate in baicalin solution were nearly 100% when using 5 and 10 kDa MWCO ultrafiltration membrane， the recovery rate of baicalin were 52.6%， 94.3%. CONCLUSION The experiments confirmed that different concentrations of activated carbon cannot effectively remove the pyrogen of baicalin solution， and the adsorption rate of baicalin was high; while ultrafiltration showed better results in pyrogen removal with high recovery rate， thus providing the basis for the production of Chinese medical injections.

Study on O/W Partition Coefficient of Chlorogenic Acid and Rats Intestinal Absorption Kinetics

BIXiao-lin, LIYao-yao, DUQiu, DILiu-qing

2013, 29(6): 572-575.

[Abstract](623) [PDF 388KB](594)

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OBJECTIVE To determine apparent partition coefficients of chlorogenic acid and to investigate its absorption kinetics in the whole small intestine and different intestinal segments of rats. METHODS The shake-flask method was employed to determine the O/W partition coefficient of chlorogenic acid， and rat single-pass intestinal perfusion technique was employed to investigate the absorptive kinetics of chlorogenic acid. RESULTS The partition coefficient(P) of chlorogenic acid was 26.07（logP1.42）；There were a little significant difference (P<0.05) between the value of Peff of chlorogenic acid at three concentrations (10， 20 and 40 μg/mL)， and no significant difference between different intestinal segment. CONCLUSION There is little difference in apparent partition on coefficient in acidic and neutral phosphate buffer solution. Besides passive diffusion mechanism， other transports methods also worked， and chlorogenic acid could be absorbed in rats' every intestinal segment.

Study on the Finger-print of Fructus Xanthii's Water-soluble Ingredients by LC-MS/MS

BAILu, LIWei-dong, CAIBao-Chang, LEIYu, XUSheng-qiu

2013, 29(6): 576-578.

[Abstract](572) [PDF 736KB](611)

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OBJECTIVE To study the chemical components of Fructus Xanthii's water-soluble ingredients. METHODS The finger-prints of Fructus Xanthii's water-soluble ingredients were established and the chemical components were identified by the method of LC-MS/MS. RESULTS Fructus Xanthii's five kinds of phenolic acids'chemical belonging were identified， and the structures were speculated. They are caffeoylquinic acid and dicaffeoylqcinic acid. CONCLUSION The identification of the chemical components of Fructus Xanthii can significantly enhance the accuracy and specificity of quality control. The qualitative and quantitative study of its chemical components can effectively control the quality of Fructus Xanthii and the relevant products.

Study on Simultaneous Determination of Saikosaponin a， Aloe-emodin， Rhein， Emodin， Chrysophanol， Emodin and Hesperidin in Xiaozheng Pills with HPLC

ZHAO Xiao-li, GONG Lei

2013, 29(6): 579-581.

[Abstract](625) [PDF 382KB](637)

Abstract:
OBJECTIVE To apply HPLC for simultaneous determination of Saikosaponin a， aloe-emodin， rhein， emodin， chrysophanol， emodin and hesperidin in Xiaozheng pills. METHODS An HPLC method was established. Waters Xbridge C₁₈ column（4.6 mm×250 mm，5 μm）was used as analytical column with a mobile phase consisting of acetonitrile (A)-0.3% phosphoric acid (B). The flow rate was 0.8 mL/min， detection wavelengths λ₁ = 210 nm and λ₂ = 300 nm and the column temperature 40 ℃. RESULTS The seven ingredients all reached baseline separation and were determined. CONCLUSION HPLC method is accurate， reliable and repeatable， which can provide a more comprehensive basis for quality control of Xiaozheng pills.

Study on Optimize Technology of Diminishing Inflammation and Relieving Itching Lotion

YANGJin, QIAOGuang-jun, MOGuo-dong, LUZhi-jun, LITian-peng, LIUYou-wei

2013, 29(6): 582-584.

[Abstract](622) [PDF 349KB](653)

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Professor ZHOU Zhongying's Experience on Treating Chronic Obstructive Pulmonary Disease

WANG Zhi-ying, JIN Lu

2013, 29(6): 585-587.

[Abstract](679) [PDF 353KB](775)

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The essay summarizes the experiences on treating chronic obstructive pulmonary disease (COPD) from Prof. ZHOU Zhongying， one of the traditional Chinese medicine masters. According to the characteristics of this disease， corresponding treating methods were raised from several aspects， such as internal and external pathogenic factor， addressing both the symptoms and causes， intermingled cold and heat， stubborn phlegm stagnation， blockage of phlegm and blood stasis and several organs getting sick at the same time. And some classical cases are illustrated.

Professor XU Fusong's Clinical Experiences on Treating Sperm Abnormality Sterility

TANG Zhi-an, JING Tao, OU Zhuo-rong, JIN Bao-fang

2013, 29(6): 588-590.

[Abstract](658) [PDF 349KB](997)

Abstract:
This paper introduced Professor XU Fusong's understanding on the etiology， pathogenesis， and main points of diagnosis and treatment of sperm abnormality sterility. 3 representative cases were analyzed to summarize his clinical experiences， including syndrome of spleen and kidney deficiency， syndrome of dampness-heat diffusing downward and syndrome of deficiency of yin essence.

Clinic Study on Niaoduqing Combined with Huangkui Slowing down the Progression of Chronic Renal Failure at Early Stage

LIU Shan, ZHANG Ming-xia

2013, 29(6): 591-593.

[Abstract](614) [PDF 350KB](693)

Abstract:
OBJECTIVE To observe clinical effect of combination of Niaoduqing Granule and Huangkui on slowing down the progression of chronic renal failure during early stage. METHODS 116 cases diagnosed with early chronic renal failure were randomly divided into control group(58) and treatment groups A(28) and B(30)， respectively treated with Aixite， Niaoduqing Granule and combination of Niaoduqing Granule and Huangkui. The following indices were detected before and eight weeks after treatment， including serum creatinine(Scr)， endogenous creatinine clearance rate (Ccr)， blood urea nitrogen (BUN)， 24h urine protein， total cholesterol (TC)， triglyceride (TG)， high density lipoprotein (HDL-C)， low density lipoprotein (LDL-C). RESULTS After 8 weeks' treatment， group A and group B showed significant differences in all indices compared with those before treatment (P<0.05). Compared to the control group， there were significant differences of 24h urine protein， TC， TG， HDL-C and LDL-C in group A and B (P<0.05， P<0.01). There was significant difference between group A and B (P<0.05). As for clinical effect， group A and B showed significant difference， compared with control group (P<0.05). There was significant difference between the two groups (P<0.05).CONCLUSION Niaoduqing Granule combined with Huangkui is more effective to slow down the progression of chronic renal failure than Aixite by intervening the reversible factors causing malignant progression of renal function， such as excreating urine protein， regulating blood fat， reducing serum creatinine and blood urea nitrogen.

Clinical Observation of Hyperlipemia Treated by Pushen Capsule

YU Guo-li, YAN Hui, HU Ming-ming, ZHENG Fang-sheng

2013, 29(6): 594-595.

[Abstract](1607) [PDF 265KB](722)

Abstract:
OBJECTIVE To investigate the clinical effective of patent Chinese drug Pushen Capsule treating hyperlipemia， and to demonstrate its function of lipid regulation. METHODS 120 cases of hyperlipemia patients were randomly divided into 3 groups (40 cases each). Group A was the Pushen capsule group and taken Pushen capsule (4 pills of each time， three times a day); Group B was the Rosuvastatin calcium tablet group (Crestor group) taken Crestor 10mg each night; Group C was combined treatment group and taken all the drugs of the upper groups. After 6 and 10 weeks， the lipemia regulation function of each groups were investigated. RESULTS Compare with before the treatment， each groups' TC/TG/TDL-C were declined remarkably after 6 and 10 weeks treatment (P<0.01)， and HDL-C were risen prominently (P<0.01). 10 weeks total effective rate comparison shows that Groups A and C were higher than B on TG， group B and C were higher than A on TC/ LDL-C. The differences were significant (P<0.05). There was no significant difference on HDL-C in three groups (P>0.05). Adverse reactions during treatment of three groups were mild. CONCLUSION total effective rate of declining TG of Pushen capsule Group is higher than Crestor Group， and combined treatment group is more effective than Pushen capsule only or Crestor only group on treating hyperlipemia. Crestor's total effective rate is higher than Pushen capsule group on lower TC/LDL-C.

Warming Kidney to Dissolve Phlegm Granule on Pulmonary Function and Quality of Life of Stable-stage Patients with COPD

GUOJie, WULei, TIANZhen-feng, JIALin

2013, 29(6): 596-598.

[Abstract](611) [PDF 341KB](700)

Abstract:

49 Cases of Anchang Ningfu Decoction Treating on Diarrhea-predominant Pattern Irritable Bowel Syndrome

ZHANG Xiao-yuan

2013, 29(6): 599-600.

[Abstract](659) [PDF 295KB](738)

Abstract:
OBJECTIVE To observe the clinical curative effect of Anchang Ningfu Decoction for diarrhea-predominant pattern irritable bowel syndrome (liver-stagnation and spleen-deficiency syndrome). METHODS 94 patients of diarrhea-predominant pattern irritable bowel syndrome (liver-stagnation and spleen-deficiency syndrome) were randomly divided into two groups. Treatment group was treated with Anchang Ningfu Decoction， and control group was orally taken Pinaverium Bromide Tablets (4 weeks for a course)， and then changes in clinical efficacy were observed. RESULTS Compare with control group， treatment group's total effective rate and single integral are significantly different (P<0.05); the total effective rate of treatment group was 93.88%， and the control group was 86.67%; the clinical efficacy of treatment group is better than control group(P<0.05). CONCLUSION Anchang Ningfu Decoction has a good effect on diarrhea-predominant pattern irritable bowel syndrome (liver-stagnation and spleen-deficiency syndrome).

2013 Vol. 29, No. 6

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