2013 Vol. 29, No. 5

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Efficacy of Nourishing Yin and Generating Body Fluid in Treating Epidemic Febrile Disease
MAHong, FUZi-yun, YANGJin
2013, 29(5): 401-404.
[Abstract](674) [PDF 416KB](745)
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Studying on Ye Tianshi's Medication Treating on Stomach Pain
LI Yong-liang, QIN Zu-jie, TANG Zhen-yu
2013, 29(5): 405-407.
[Abstract](719) [PDF 351KB](727)
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Ye Tianshi is a very famous doctor in Qing dynasty， who paid attention to activating yang to getting rid of turbidity and regulating qi activity in stomach treatment. He emphasized that treating spleen and stomach separately， at the same time for the long time pain and the disease into meridian they should promoting blood circulation to remove blood stasis， etc. He had put forward a lot of insight ideas. In this paper， the author studied Ye Tianshi's medication principles， hoping to provide reference to the treatment of stomach and spleen disease.
Developing Guidelines for the Application of Delphi in Evidence-based Clinical Practice of TCM
CHEN Zheng-guang, WANG Shou-chuan, LUO Hui, ZHAO Xia, XU Shan
2013, 29(5): 408-411.
[Abstract](654) [PDF 341KB](714)
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Delphi was thought to be an important method in developing the clinical practice guidelines(CPG) of evidence-based in Traditional Chinese Medicine(TCM) by researching the methodology of developing CPG of TCM. This paper discussed the position and function， specific application and may involve aspects of Delphi in developing CPG of TCM， and pointed out some problems on the development of clinical practice guidelines by using Delphi in evidence-based TCM.
External Fixation Support Versus Small Splint Fixation in Unstable Distal Radius Fractures: a Meta-analysis
CHENG Jian, GUO Yang, MA Yong, BAO Jun-chen, WANG Yu-chen
2013, 29(5): 412-417.
[Abstract](587) [PDF 417KB](596)
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OBJECTIVE Through meta analysis to compare the effect of external fixation support versus small splint fixation in unstable distal radius fractures regarding clinical results， radiological outcomes and postoperative complications. METHODS Cochrane Library， MEDLINE， EMbase， CBM and the relevant English and Chinese orthopedic journals were searched for the randomized controlled trials of external fixation support versus small splint fixation in unstable distal radius fractures. Then the researchers analyzed wrist functions， radiological outcomes and complications though meta analysis. RESULTS A total of 8 RCTs with a total of 713 participants were included. The results of Meta-analyses showed that compared with small splint fixation fixation， wrist functions[RR=1.25， P<0.01] and radiological outcomes[RR=1.24， P<0.01] were superior in the external fixation support group， meanwhile， the loss of volar tilt angle[MD=4.85，P<0.01] and ulnar inclination angle[MD=3.20， P<0.01] were smaller and the length of radial shortening[MD=-3.42， P<0.01] was shorter after six months follow-up. And external fixation support fixation led to significantly fewer total complications[RR=0.62， P<0.01]. CONCLUSION External fixation support is a better alternative to small splint fixation for unstable distal radius fractures. However， more high quality， large scale and multicentric randomized controlled trials are required for more accurate conclusion.
Discussion on Modern Clinical Bibliometric Analysis and Its Characteristics of Grain-sized Moxibustion
XUYan, DONGQin, XUTian-shu
2013, 29(5): 418-421.
[Abstract](686) [PDF 393KB](574)
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OBJECTIVE To explore the disease spectrum of grain-sized moxibustion and its characteristics. METHODS By reviewing clinical literature， certain literature about grain-sized moxibustion published from 1980 to 2012 were collected from China National Knowledge Infrastructure (CNKI). RESULTS The clinical application of grain-sized moxibustion has been increased gradually and the range of diseases it can treat is wider， reaching more than 40 different kinds related to many clinical departments. It is commonly used in nerve， mental and digestive system diseases， as well as diseases of orthopaedics and dermatology. After specific analysis of statistics， it is mostly used in the treatment of arthritis. CONCLUSION The grain-sized moxibustion has the function of obvious dual-directional regulation: it may dispel cold and also may purge heat; may reinforce insufficiency but also reduce excessiveness. The grain-sized moxibustion may have a good effect on difficult miscellaneous diseases.
Clinical Efficacy Evaluation and Its Influential Factors of Gua Sha Therapy in Treatment of Lumbar Muscle Strain
WANGYing-ying, YANGLi, YANGJin-sheng, YANGJin-hong, LIUZhi-bin, CHENFeng, LIUDong-xia, YUANHai-guang
2013, 29(5): 422-425.
[Abstract](688) [PDF 381KB](600)
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OBJECTIVE To discuss the basic factors influencing curative effects of Gua Sha (at acupoints or along meridians) and explore relations of curative effects with site and strength. METHODS 210 patients with lumbar muscle strain were divided into 5 groups with 42 cases in each group， namely the group of Gua Sha at acupoints with ecchymosis， the group of Gua Sha therapy along channels with ecchymosis， the group of Gua Sha at acupoints without ecchymosis， the group of Gua Sha therapy along channels without ecchymosis and the group of acupuncture. The 5 groups were treated for 7 times successively. VAS scores， ODI scores and curative effects of the 5 groups were recorded before and after each treatment and during follow-up visits which were one month and three months after treatment. RESULTS VAS scores and ODI scores of the 5 groups after treatment were obviously improved (P<0.01) and the curative effects were reliable during follow-up visits one month and three months after treatment and the group of Gua Sha therapy along channels with ecchymosis had the best curative effect(89.7%). The ecchymosis and scraping sites are the main influential factors of curative effects by Logistic stepwise regression analysis. CONCLUSION Gua Sha therapy has significant effects on the symptoms of lumbago. The ecchymosis and manipulating Gua Sha therapy along channel are the main factors affecting the corresponding curative effects.
Observation on the Effect of Shenkang Injection on Preventing Contrast-induced Nephropathy after Senile Coronary Heart Disease Interventional Surgery
WANG Xin-dong, SHEN Jian-pin, LOU Bin
2013, 29(5): 426-428.
[Abstract](637) [PDF 343KB](708)
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OBJECTIVE To observe the on the effect of Shenkang Injection on the incidence of acute kidney injury and contrast-induced nephropathy after interventional surgery for senile patient with coronary heart disease. METHODS 62 cases of senile coronary heart disease who had interventional examination and treatment were randomly devided into two groups， namely observation group ( Shenkang group， 31 cases ) and control group (Hydration group， 31 cases). The two groups both received rutine treatment. The observation group received intravenous infusion of Shenkang Injection. The control group received hydration treatment. Blood samples of all the patients were taken to test their serum creatinine， and neutrophil gelatinase associated lipocalin (NGAL).The incidence of contrast-induced nephropathy of these two groups were compared. RESULTS 1 case in Shenkang group(3.2%) and 4 cases in Hydration group (12.9%) had CIN after surgery and there was a statistical significance between the two groups (P＜0.05). There was no statistical significance between the comparisons of the two groups’ SCr and eGFR point after surgery(P＞0.05). 12 hours and 24 hours after the surgery， the neutrophil gelatinase associated lipocalin (NGAL) of both groups increased while the increase in Hydration group was greater compared with Shenkang group with a statistical significance (P＜0.05). Blood SCr value of Shenkang group at any test point after surgery was lower than that of Hydration group with a remarkably statistical significance ( P＜0.01). CONCLUSION Shenkang Injection may have a good effect on preventing the incidence of acute kidney injury and contrast-induced nephropathy after interventional surgery for senile patient with coronary heart disease.
Effects of Zhen Zao Capsule on 5-HT and DA of Senile Patient with Yin-deficiency and Fire-excess Insomnia
HAN Xu, LI Qi-Yi, GAO Hong-min, GAO Jue-min, YU Gu-ran, JIANG Meng, JU Wen-zheng
2013, 29(5): 429-431.
[Abstract](806) [PDF 354KB](740)
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OBJECTIVE To observe the clinical changes， 5-HT and DA of Zhen Zao Capsule in the treatment of senile patients with yin-deficiency and fire-excess insomnia， thus evaluating the clinical effects and functional mechanism of Zhen Zao Capsule. METHOD 60 cases with yin-deficiency and fire-excess primary insomnia were randomized into the treatment group (30 cases) given Zhen Zao Capsule half an hour before sleep， and the control group (30 cases) treated with Shumian Capsule at the same time. Both groups were given 5 pills per time and one time a day. 4 weeks made up one course of treatment. The two groups were observed every 7 days for one month. Detecting the changes of 5-HT and DA as well as evaluating the clinical effects of two groups. RESULTS The clinical effects of relieving insomnia in both groups were equal (P>0.05). However， in terms of improving the TCM syndromes and effects， the treatment group was superior to the control group (P>0.05). Before and after treatment， 5-HT increased while DA decreased. Amplitude of accommodation in the treatment group was significant than that of the control group， which was of statistical significance (P>0.05). CONCLUSION With significant clinical effects， Zhen Zao Capsule can improve insomnia in senile patients. The treatment mechanism maybe achieved by increasing the 5-HT and lowering DA， thus improving the sleep quality of senile patients.
Influence of Asthma-reliving Decoction on Pulmonary Function and Clara Cell Secretion Protein of Children with Asthma
LI Yi-min
2013, 29(5): 432-434.
[Abstract](611) [PDF 331KB](585)
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OBJECTIVE To study the influence of asthma-reliving decoction on pulmonary function and Clara cell secretion protein (CC16) of children with asthma. METHODS 120 children with asthma were randomly divided into control group (60 case) and treatment group (60 cases). Each group was first received with Seretide inhalation therapy. Treatment group were given with asthma-reliving decoction additionally. After 3 courses， the therapy effect was evaluated. And then， the pulmonary function index (FEV1 and PEF) and the levels of CC16， IgE and eosinophil cationic protein (ECP) were observed before and after the treatment. RESULTS After the treatment， the pulmonary function in treatment group was obviously improved. The level of CC16 increased and IgE and ECP decreased in each group. And ones in treatment group were more effective than that of control group. The clinical efficiency of treatment group was 91.67%， which was obviously higher than that of control group. CONCLUSION The asthma-reliving decoction can improve pulmonary function obviously and shows a certain regulation of CC-16， IgE and ECP， which may play the role of relieving cough and asthma.
Exploration on the Effect of Foot Reflex Zone Massage on Defecation Function of Postoperative Patients with Low Anterior Resection
YANGLiu, LIHong, WANGLong, HUANGGui-fang
2013, 29(5): 435-438.
[Abstract](634) [PDF 390KB](625)
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OBJECTION To explore the effect of foot reflex zone massage on defecation function of postoperative patients with low anterior resection. METHODS 47 patients with low anterior resection were randomly divided into treatment group with 23 cases(2 cases were lost) and control group with 24 cases(1 case was lost).The patients in experimental group were treated by foot reflex zone massage for 3 months and received routine care daily. The control group received routine care daily. Evaluation of defecation function in patients with postoperative 1 month， 2 months， 3 months by Xu's scale of anorectal function. RESULTS Postoperative 1 month， 2 months， there was no significant difference in defecation function between two groups (P>0.05); Postoperative 3 months， treatment group defecation function was improved obviously， compared with control group with significant difference(P<0.05). CONCLUSION Massage of foot reflex zone can improve the defecation function of postoperative patients with low rectal anastomosis.
Effects of Bushen Yisui Capsule on Expression of BDNF and Nogo A in Mice with Experimental Autoimmune Encephalomyelitis
ZHENGQi, YANGTao, FANGLing, ZHAOHui, FANYong-ping, WANGLei
2013, 29(5): 439-444.
[Abstract](642) [PDF 428KB](569)
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OBJECTIVE The previous study had shown that Bushen Yisui Capsule played an important role in the development and prevention of experimental autoimmune encephalomyelitis (EAE). In present study， the effects of Bushen Yisui Capsule on the expression of brain-derived neurotrophic factor (BDNF) and Nogo A in mice with EAE were discussed.METHODS The C57BL/6 mice were induced by immunization with myelin oligodendrocyte glycoprotein (MOG) 35-55. The body weights and neurological function scores of mice were examined everyday. The pathological changes in the brains of mice were observed with hematoxylin-eosin (H&E) staining， while the protein expression of BDNF and Nogo A were measured by immunohistochemistry (IHC) on Day 18 and Day 40 post-immunization (PI).RESULTS The data showed that inflammatory cells were reduced in the treatment group. The expression of BDNF obviously increased in the cerebral cortex (P<0.01)， the inferior to third ventricle (P<0.01) and the around of lateral ventricle (P<0.01) on Day 18 and Day 40 PI. In addition， the expression of Nogo A obviously decreased in the different areas of brain above (P<0.05， P<0.01).CONCLUSION Bushen Yisui Capsule has neuroprotective function in the mice with EAE， and the mechanism may be related with increasing expression of BDNF and decreasing expression of Nogo A.
The Influence of Guipi Decoction on the Depression Rats Serum Contents of T3， T4 and TSH
CHEN Bao-zhong, WANG Qing-shuang, LI Yi, YAO Dan, GAO Yan-yu, HU Xiao-yang, Bi Jun-hui, TANG Ming-zhe
2013, 29(5): 445-447.
[Abstract](620) [PDF 325KB](716)
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OBJECTIVE To explore the mechanism of Guipi Decoction treating on the depression rats through the measurement of rats serum contents of Triiodothyronine， Thyroxine and Thyrotropin changes.METHODS The depression rat model of depression were established by chronic stress method. Experimental animals were divided into regular group， model group， optimal solution group and Guipi Decoction high， medium and low dose groups. Rats body weight were observed， and open boxes in accordance with experiments conducted on rats behavior detection. Till 42 day in the experiment the rats were sacrificed， femoral artery blood， separation of plasma， serum， measured by radioimmunoassay using rat plasma T3， T4 and TSH levels.RESULTS Compared with the regular group， the model group has slower pace of growth of body weight， the scores of horizontal movement and vertical movement reduced， blood T4 levels reduced， T3 and TSH levels have remarkable differences. Guipi Decoction in each group and the group optimal solution to increase the growth rate of body weight and movement of behavior science scores， and can add the blood T4 levels.CONCLUSION Guipi Decoction can add the blood T4 levels on chronic stress-induced irritation rats and as the role of an antidepressant， which may be corrected through the HPT-axis to achieve.
Experimental Research into Tounong Powder Preventing Formation of Chronic Peptic Ulcer Scar
CHENJi-lan, ZHANGHui-hui, XUMeng, YEQiao-bo, HUANGXiu-shen, WANGLiang
2013, 29(5): 448-451.
[Abstract](611) [PDF 397KB](584)
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OBJECTIVE To investigate Tounong Powder's optimizing fibroblast's function and preventing the formation of chronic peptic ulcer scar through its effect on the content of growth hormone (GH)， hydroxyproline (Hyp) and hyaluronidase (HAase).METHODS Acid burning chronic gastric ulcer model was established. The rats were divided into 8 groups: blank control group， model group， high， middle and low-dose of Tounong Powder groups， western medicine control groups (omeprazole group， ranitidine group and Hydrotalcite group). ELISA was applied to detect the GH， Hyp and HAase levels. RESULTS 14 days after administration， compared with model group， GH levels of all the Tounong Powder groups decreased， while HAase increased. Hyp of high-dose group went up， but in low and middle-dose groups it was reduced.CONCLUSION Tounong Powder lowers GH and Hyp levels and elevates HAase level， thereby optimizing fibroblast function to prevent chronic gastric ulcer scar shaped. Its effect in some respects is better than the control.
Effects of Xijiao Dihuang Decoction on the TNF-α， IL-6 and Hemorheology of Stagnated-heat Syndromes and H-hypertensive rats
LIUHong-quan, HUANGHou-cai, ZHONGRong-ling, XIAZhi, GUOLi-zhong, WANGSheng
2013, 29(5): 452-454.
[Abstract](669) [PDF 346KB](581)
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OBJECTIVE To investigate the effects of Xijiao Dihuang Decoction on the TNF-α， IL-6 and hemorheology of stagnated-heat syndromes (elevated homocysteine levels-Hcy accompanied with hypertension ) and H-hypertensive rats.METHODS Modeling stagnated-heat syndromes and H-hypertensive rats， then they were treated with three different doses Xijiao Dihuang Decoction respectively (0.75 g crude drug/mL，1.5 g crude drug/mL， 3.0 g crude drug/mL) as well as a control group with no treatment. To detect and analyze the TNF-α， IL-6， and hemorheology statistically.RESULTS The TNF-α， IL-6 and hemorheology in the treatment group were significantly different compared with the control group. The difference was statistically significant (P<0.01)， with low and middle dose more effective.CONCLUSION Xijiao Dihuang Decoction can effectively intervene the TNF-α， IL-6， and hemorheology of stagnated-heat syndromes and H-hypertensive rats， thus achieving the simplification of complex risk factors for stroke in clinical studies.
Discussion on the Effects of Liangxue Huayu Fang on Autophagy and Apoptosis of Protein Associated Hepatocyte Injury and Its Molecular Mechanism
HUXin, CHENWei-ping, YANXiao-jing, JIANGZe-qun
2013, 29(5): 455-458.
[Abstract](730) [PDF 373KB](605)
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OBJECTIVE To study the effects of Liangxue Huayu Fang on pathways related to autophagy and apoptosis of hepatocyte injury induced by tumor necrosis factor alpha (TNF-α) combined with D- galactosamine(D-GalN)， and to explore the molecular regulative mechanism.METHODS The normal human liver cells LO2 were cultured in vitro. The hepatocyte injury model was induced by TNF- α (100 ng/mL) and D-GalN(44 μg/mL). Fluorescence microscope was used to observe the morphological changes of cells. The cell viability was detected by MTT; Western blot was adopted to detect the apoptosis and autophagy marker， such as proteins caspase-3， Beclin1， LC3 Ⅰ， LC3 Ⅱ; changes of mTOR protein expression level of apoptosis and autophagy regulating protein Bax， Bcl-2 and mTOR signal pathway were also detected.RESULTS Liangxue Huayu Fang can improve pathological injury of hepatocyte with a dose-dependence; cell apoptosis was decreased and the expression of autophagy markers， such as protein caspase-3， Beclin1， LC3 Ⅱof the treatment group showed decreased and the expression of negative correlative Bcl-2 protein was up-regulated while the positive correlative Bax expression was down-regulated. All the data showed a dose - response relationship to some degree and the expression of mTOR protein showed down-regulated with dose - response.CONCLUSION The protective effects of Liangxue Huayu Fang on hepatocyte injury induced by TNF-α and D-GalN may be related to inhibition of autophagy and apoptosis. Its main mechanism may relate to regulation of the expression of proteins participating in autophagy and apoptosis.
Establishing the Rabbit Model of Degenerative Intervertebral Disc by Annulus Puncture
DUYan-xin, TONGDe-min, DENGJin-feng, GUOYu-hai, CHENBo-lai, XUANCui-ling, FULu-di, TANDi-qing
2013, 29(5): 459-461.
[Abstract](697) [PDF 358KB](563)
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OBJECTIVE To establish the rabbit model of degenerative intervertebral disc by annulus puncture.METHODS Forty New Zealand white rabbits were randomly divided into 2 groups. Operation group was for annulus fibrosus with a 16-gauge needle damaging the right front side of lumbar vertebrae in 30 New Zealand white rabbits while the rest ten rabbits were used as control group without any annulus puncture. The changes in intervertebral space and pathological conditions were respectively examined with lateral spine X-rays and histopathological exam ination on 4， 8 and 12 weeks after operation.RESULTS The height of intervertebral space in operation group was gradually decreased and the difference of the rate of change of DHI between the two groups was statistically significant from the fourth week on (P<0.05). The histological sections demonstrated that the cell content in nucleus pulposus in operation group gradually decreased.CONCLUSION The annulus puncture may slowly authentically initate human disc degeneration after injury and the modeling method is safe and reliable.
Effects of Sophocarpine of Rats Acute Myocardial Ischemia Induced by Coronary Artery Ligation
DI Ling, LI Rui-Ping, YANG Cheng-zhi, LIU Su-rong, YANG Hai-xia
2013, 29(5): 462-465.
[Abstract](752) [PDF 392KB](596)
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OBJECTIVE To observe effects of sophocarpine to SOD， MDA and Connexin43 in acute myocardial ischemia rats induced by coronary artery ligation，to explore the mechanism of action of the sophocarpine and to provide laboratory evidence for the application of sophocarpine.METHODS The experimental animals which were divided into sham operation， model group， amiodarone group and different doses sophocarpine groups were administrated for 7 days，and were ligated the left coronary artery to induce SD rats myocardial ischemia， II lead electrocardiogram was recorded before， during and after model establishment. Blood from femoral artery was collected and its serum was separated to detect the content of SOD and MDA. The ischemic part of the rat's heart was selected for histological examination. Immunohistochemistry was applied to observe the distribution of CX34.RESULTS ① Compared with the sham group， SOD decreased significantly (P<0.01)， while MDA was significantly higher (P<0.01). Compared with the model group， amiodarone， sophocarpine middle dose group， sophocarpine big dose group SOD was significantly higher (P<0.05)， amiodarone， sophocarpine high-dose group MDA decreased significantly (P<0.05 ). CX43 expression within the cytoplasm. ②Compared with the sham group， model group， ST-T immediately after modeling and 24 hours was significantly higher (P<0.01). Compared with the model group， amiodarone group sophocarpine middle dose group， the high dose group of sophocarpine， after modeling instantly ECG ST-T was significantly depressed (P<0.01)， ST-T sophocarpine low-dose group depression (P<0.05). ③ Myocardial fibers within the cytoplasm of sham-operated and treated Cx43 expression compared with the model group， arranged chaos， amiodarone group， small-dose group of sophocarpine ， middle-dose group of sophocarpine ， high-dose group of sophocarpine， the average optical density value decreased significantly (P<0.01).CONCLUSION The sophocarpine can increase the content of serum superoxide dismutase， reduced malondialdehyde content， against myocardial ischemia-induced abnormal distribution of Connexin43.
Effects of Ginger Moxibustion on VIP and IL-10 in Gastric Cancer Rats
JIANGWei, YUGuo-hua, CHENJian-zhang, TANGXiang-yu, GONGXiao-jun, ZHANGHong-quan, WUJuan, WANGJin
2013, 29(5): 466-468.
[Abstract](591) [PDF 339KB](636)
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OBJECTIVE To observe the effect of ginger moxibustion on vasoactive intestinal peptide(VIP) and interleukin-10(IL-10) in gastric cancer rats.METHODS The gastric carcinoma models， established by N-methyl-N-nitro-N-nitrosoguanidine(MNNG)， were randomly divided into 4 groups: VIP group(n=10， 10 μg/100 mL)，VIP antagonist group(n=10， 10 μg/100 mL)， model group(n=10， the normal breeding) and ginger moxibustion group(ginger moxibustion， n=10， on zhongwan， pishu， weishu， zusanli， neiguan， etc). Apart from that， blank group(n=10， physiological saline) was set up additionally. Three weeks later， the contents of VIP， IL-10 were tested， and the expression of proteins VIP and mRNA in gastric cancer tissues were detected by immunohistrochemistry and RT-PCR methods.RESULTS VIP and IL-10 in model group and were reduced， and the ones in ginger moxibustion group were increased obviously(P<0.01)， which have significant differences with the VIP group. Gastric carcinoma group have VIP-positive immunoreactive cells， which were not found in normal gastric tissues. There was VIP mRNA expression in each groups' gastric cancer tissue. The expression of mRNA in Ginger moxibustion and VIP groups were reduced， but ginger moxibustion group was more significantly reduced(P<0.01).CONCLUSION Gastric cancer rats can be treated with ginger moxibustion by inhibiting the detection of VIP， IL-10 and the expression of VIP mRNA.
Experimental Research on Protection of Replenishing Kidney Essence with Electroacupuncture (EA) on Brain in Perimenopausal Rats
XU Tian-shu, ZHANG Hua-jun, ZHAO Hang, LIU Ying-lu, WANG Yu-juan
2013, 29(5): 469-472.
[Abstract](629) [PDF 386KB](583)
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OBJECTIVE To observe effects of replenishing kidney essence with electroacupuncture and estrogen replacement on vivo SOD， MDA and caspase-3 in ovariectomized rats after cerebral ischemia and contrast the cerebral protection in ovariectomized rats with the two pretreatment methods.METHODS 60 female SD rats were randomly divided into four groups， namely， sham group， castration alone group， and estrogen pretreatment group and EA pretreatment group. Three groups except sham group all underwent bilateral ovariectomy (OVX) to establish perimenopausal rat models. EA pretreatment rats were punctured in CV4 and bilateral SP6 points with replenishing kidney essence while estrogen pretreatment group was intramuscularly injected with estrogen estradiol benzoate (0.15 mg/kg). All four groups were given the same bundle processing with the same time as EA pretreatment group. After 24 hours treatment， they were operated with cerebral ischemic to make middle cerebral artery occlusion (MCAO) models and reperfused 2 hours later. In 24 h， the rats were killed and blood and brain tissue were removed for NSS score and calculating the infarct volume and E2， SOD， MDA and caspase-3 content within specimens.RESULTS SOD in blood and brain tissue in EA pretreatment was significantly higher than that in estrogen pretreatment group (P<0.05)， MDA level was significantly lower than the latter group (P<0.05)， caspase-3 levels between the two groups without significant difference (P>0.05).CONCLUSION Replenishing kidney essence method with EA can protect the brain of perimenopausal rats and electrical stimulation makes a more markedly effect.
Research on Mechanism of Qingfei Pingchuan Capsule Treating Asthma
JIN Xuan, ZHAO Xue-long
2013, 29(5): 473-475.
[Abstract](663) [PDF 308KB](570)
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OBJECTIVE To explore the mechanism of Qingfei Pingchuan Capsule treating on asthma by discussing its function on asthma rats' IL-4， IL-13 cytokines， IgE inflammatory mediator， lipid peroxide MDA. METHODS The asthma rats' model were established by allergen， then the rats' BALF and IL-4， IL-13， IgE， MDA in serum were tested respectively.RESULTS Compared with saline group， the model group rats' BALF and IL-4， IL-13， IgE， MDA in serum were significantly higher(P<0.01， P<0.05)， and the contents of IL-4 in BALF in the model group have the positive correlation with the contents of MDA and IgE(r=0.83， 0.96， P<0.05). Compared with the model group， treatment group rats' BALF and IL-4， IL-13， IgE， MDA in serum were significantly lower(P<0.01， P<0.05)， and the contents of IL-4 in BALF in the model group have the positive correlation with the contents of MDA and IgE(r=0.86， 0.88， P<0.05).CONCLUSION Qingfei Pingchuan capsule can bring down the expression of MDA， IL-4， IL-13 cytokines， reduce the increasing inflammatory IgE， prevent asthma inflammation， relieve airway inflammation and ease its high-response， and avoid the aggravation of asthma.
The Allergic Substances in Danshen Drip
CHENG Jian-ming, ZHU Dan-feng, SHUAI Wei-wei, JIANG Bao-ping, PENG Guo-ping
2013, 29(5): 476-478.
[Abstract](617) [PDF 320KB](676)
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OBJECTIVE To track the allergic substances in Danshen Drip. METHODS 5-hydroxy tryptamine and ovalbumin were taken as index and positive drug respectively to optimize the sensitization time and attacking time in order to obtain the optimized animal model through which the allergic substances in Danshen Drip was selected. RESULTS Each guinea pig was subcutaneously injectioned with 0.2 mL Danshen Drip on alternate days for three times， 16 days after the first injection， each guinea pig was undertaken subcutaneous injection with 0.5 mL for repeated attack.CONCLUSION The allergic substance of Danshen Drip is salvia miltiorrhiza protein.
Simultaneous Determination of Flavones and Scutebarbatines in Scutellariae barbatae by HPLC
KANGAn, QIAOGuang-jun, GUOJin-rui, ZHANGZheng-wei, DILiu-qing, WUMian-hua, ZHOUHong-guang
2013, 29(5): 479-481.
[Abstract](662) [PDF 322KB](678)
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OBJECTIVE To establish HPLC methods for simultaneous determination of luteolin， apigenin， wogonin， scutebarbatine A and scutebarbatine B.METHODS The analytical column was Phenomenex Luna C18(250 mm×4.60 mm， 5 μm). The mobile phase was composed of acetonitrile and water with gradient elution. The flow rate was 1.0 mL/min; The column temperature was 30 ℃. Luteolin and apigenin were detected at 355 nm; wogonin， scutebarbatine A and scutebarbatine B were detected at 264 nm.RESULTS The ranges of calibration curve of luteolin， apigenin， wogonin， scutebarbatine A and scutebarbatine B were 0.037 4~2.40 μg、0.031 2~2.00 μg、0.031 2~2.00 μg、0.006 2~0.40 μg and 0.006 2~0.40 μg， respectively. Also， the intra-batch and inter-batch variations and the average recoveries were all meet the requirement of Chinese Pharmacopoeia.CONCLUSION The method is rapid and simple with a good reproducibility and it can be used for the quality control of herba Scutellariae barbatae.
Analytical Study on the Flavonoids Constituents in Herba Hyperici Japonici by UPLC-MS
LU-li, HANLe, ZOULi-si, LIUXun-hong
2013, 29(5): 482-485.
[Abstract](613) [PDF 345KB](718)
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OBJECTIVE To establish an Ultra Performance Liquid Chromatography (UPLC-MS) method to analyze the flavonoids constituents in Herba Hyperici Japonici.METHODS The analysis was performed on an Alltima C18 chromatographic column with a gradient solvent system of acetonitrile-0.8% acetic acid at a flow rate of 0.8 mL/min. The detection wavelength was 254 nm and the column temperature was 30 ℃. The data was collected under the negative electrospray ion mode.RESULTS 8 flavonoids constituents being separated and identified， six of them were detected simultaneously. The result shows the detected flavonoids constituents have good linearity between concentration and peak area (r2>0.998 2)， and the average recoveries of the method were between 97.6%~99.7%.CONCLUSION Being simple， accurate and reproducible， UPLC-MS can be used for evaluating the quality of Herba Hyperici Japonici.
Professor Meng Jingchun’s Clinical Experience of Treating Chronic Astrophic Gastritis
LIU Zhou, ZHANG Wei-hua, LUO Shu
2013, 29(5): 486-488.
[Abstract](642) [PDF 383KB](716)
Abstract:
This paper summarizes Professor Meng Jingchun’s clinical experience of treating chronic astrophic gastritis. Firstly， improve the formation of qi and blood to treat gastric mucosa atrophia， that is， tonifying deficiency for unobstruct the stagnant. Secondly， improve the internal environment of the stomach to kill Helicobacter pylori (Hp)， that is， dissolving dampness and turbid. Thirdly， judge the hour and size up the situation to prevent precancerosis of stomach， that is， proper tonification and reducing.
Brief on Geratic Cases Treated by Doctor Hu Tiecheng
GUO Hong-min
2013, 29(5): 489-490.
[Abstract](616) [PDF 311KB](668)
Abstract:
This paper introduces Doctor Hu Tiecheng’s experience of treating geriatrics including senile swelling， insomnia， night sweating and depression. Geratic swelling is usually treated according to the theory of blood stasis causes swelling; night sweating of the elderly is treated based on the theory that the sweat is derived from heart-blood; senile depression is treated by Xiaochaihu Decoction.
Zhang Zhijian’s Experience of Treating Nephropathy Hematuria
ZHAO Min, ZHANG Fu-chan, WANG Shen-jun, CHEN Dai
2013, 29(5): 491-492.
[Abstract](657) [PDF 318KB](679)
Abstract:
Professor Zhang Zhijian diagnoses and treats hematuria of nephropathy by differentiating deficiency and excess which may be due to wind heat， dampness heat or toxic heat; zang and fu organs which may be caused by qi deficiency or yin deficiency. The location of this disease is lung， spleen and kidney. Therapies like cooling heat and blood， nourishing qi and blood， strengthening spleen and kidney， clearing away dampness and heat are often applied by Doctor Zhang who also advocates treating both the root cause and symptoms of this disease. He believes stopping blood urine is of priority at the acute stage， while curing the causes is the final object at the stable stage.
Research Progress on Mechanism of Ovariogenic Hyperandrogenism of Polycystic Ovary Syndrome and Interventive Effect of Traditional Chinese Medicine
TONG Xing-li, TAN Yong
2013, 29(5): 493-496.
[Abstract](649) [PDF 386KB](664)
Abstract:
Research on Immunological Mechanisms and Regulation of Traditional Chinese Medicine in Asthma
SUNYan-zhen, YUANXue-jing, SUNYi-qiu
2013, 29(5): 497-500.
[Abstract](619) [PDF 407KB](1049)
Abstract:

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