2013 Vol. 29, No. 4

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Commonly-used Compatibility of Medicines with the Function of Promoting Blood Circulation for Removing Blood Stasis and Its Clinical Application in Treating Cancer
ZHANGYong-hong
2013, 29(4): 305-308.
[Abstract](640) [PDF 419KB](636)
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This paper is to discuss the compatibility principles and its clinical application in treating cancer of commonly-used medicines with the function of promoting blood circulation for removing blood stasis. The paper explained and analyzed the compatibility principles to remove blood stasis， namely eliminating phlegm， detoxification， regulating qi， dispelling cold， removing dampness， purgation， hemostasis， expelling wind， reinforcing qi to transform to yang and nourishing yin etc.
The Comparison Between Traumatic Fear Memory of PTSD and the Theory "Fear Being the Emotions of the Kidney"
JIAODong-liang, GAOYan
2013, 29(4): 309-311.
[Abstract](646) [PDF 369KB](1199)
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After the comparison of traumatic fear memory of PTSD and the theory "kidney is fear reflected in emotions"， the present author pointed out that this theory had significant values on treating PTSD. This paper also expounded the meaning of researching the theory through the view of central nervous circuitry and related neurotransmitter， and its scientific value and guiding significance of the theory on the modern mental psychology research.
Study of Chronic Congestive Heart Failure from the View of Water
HUANG Xu-xia
2013, 29(4): 312-313.
[Abstract](632) [PDF 358KB](868)
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The syndrome of heart failure is very numerous and disordered， and there is no clearly distribution of syndrome types. However， although there are different syndromes， they all connected with water dampness. The mechanism of chronic congestive heart failure lies on yang deficiency and water retention， bad water circulation and disturbance to the heart. The water metabolism disorder forms water evil. Water is the key factor throughout the whole mechanism process. So treating from the perspective of water can help and make hard things simple.
Clinical Study of Regulating Liver and Activating Spleen Therapy for Diarrhea-Irritable Bowel Syndrome
YE Bo, CHEN Jing, NING Li-qin, LIU Chun, CAO Ting-ting
2013, 29(4): 314-316.
[Abstract](618) [PDF 292KB](651)
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OBJECTIVE To assess the effect of regulating liver and activating spleen therapy on patients with diarrhea-irritable bowel syndrome (D-IBS) belonging to liver depression and spleen deficiency type as well as its safety.METHODS 96 cases conforming to the including criteria were randomly divided into treatment group and control group with 48 in each. 1 case of the treatments and 2 cases of the controls fell off as they did not return visit since the first consultation. The treatment group were given traditional Chinese medical formula Tiaogan Yunpi Fang for regulating liver and activating spleen， the control group were administrated with Deshute. The course of two groups was 4 weeks. Symptoms， signs and tests results of the patients before and after treatment were collected and analyzed. RESULTS The treated patients were better than control patients in total effective rate and recovery rate (P<0.05). Symptom score of both groups significantly decreased after treatment， but the treatment group was superior to the control (P<0.01). The symptom score of treatment group decreased in comparison with that before treatment (P<0.05). All the symptoms of control patients were improved except for non-complete defecation (P<0.05). However， abdominal pain and discomfort， defection frequency， feces property of the treated patients were improved significantly， much better than those of the control (P<0.05). There was no adverse reaction in two groups. Regular blood test， urine test， stool test， ALT， BUN， Scr were not found abnormal. CONCLUSION Tiaogan Yunpi Fang is effective on D-IBS with great safety.
Analysis on Relation Between Serum Ischemia Modified Albumin and Blood Stasis Syndrome of Type 2 Diabetes
SHANG Wen-bin, WANG Jin-ling, YU Xi-zhong, GAO Yan
2013, 29(4): 317-319.
[Abstract](634) [PDF 352KB](759)
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OBJECTIVE To investigate the relation between serum ischemia modified albumin (IMA) and Blood stasis syndrome of Type 2 diabetes in order to provide referential evidences for standardization of treating diabetes based on traditional Chinese medicine (TCM) syndrome differentiation. METHODS 79 subjects with Type 2 diabetes were gathered and divided into Blood stasis syndrome group(34 cases) and non-Blood stasis syndrome group(45 cases) according to the diagnostic theory of traditional Chinese medicine. Serum IMA of each patient was detected， and its relation correspondingly with age， BMI， glucolipid metabolism index (FBG， HbAlc， TC， TG， LDL-C， HDL-C) and blood pressure (SBP， DBP) were recorded and analyzed. RESULTS Compared with non-Blood stasis syndrome group， IMA， FPG and HbA1c were significantly higher in Blood stasis syndrome group with a stastistic significance. Pearse corelation analysis showed that all the patients' serum IMA was positively correlated with HbA1c (P<0.05). However， partial correlation analysis showed that excluding the influence of age， BMI， blood TC， TG， HDL-C， LDL-C， SBP， DBP， there was no significant correlation between serum IMA and FBG， as well as serum IMA and HbA1c (P>0.05). CONCLUSION The serum IMA level reflects the features of blood stasis syndrome of type 2 diabetes， and may be independent of glucose levels outside the blood stasis syndrome.Serum IMA is another objective marker of Blood stasis syndrome of Type 2 diabetes except glucose levels.
Comparative Study of Serum Metabonomics of Hypertension of Liver-Fire Hyperactivity Syndrome and Yin-Deficiency and Yang-Hyperactivity Syndrome
CHEN Yi, FANG Zhu-yuan
2013, 29(4): 320-323.
[Abstract](672) [PDF 332KB](608)
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OBJECTIVE To analyze and compare the different biomarker groups of liver-fire hyperactivity syndrome and yin-deficiency and yang-hyperactivity syndrome of hypertension and explore molecular basis of TCM syndrome of hypertension. METHODS The gas chromatography-time of flight mass spectrography (GC-TOFMS) technology was employed to analyze the serum metabonomics. RESULTS ①Glutamic oxalacetic transaminase (GOT) and triacylglycerol in group of yin-deficiency and yang-hyperactivity syndrome of hypertension is markedly higher than the group of liver-fire hyperactivity syndrome (P<0.05). ②Groups of liver-fire hyperactivity syndrome and yin-deficiency and yang-hyperactivity syndrome in hypertension are different in the principal component scores three-dimensional diagram. The different compounds which can be identified include citrate， oxalate， dioxide heptanoic acid， glycerate， soft hydrochloric acid， ethanol amine， inositol， oleic acid， stearic acid， aspartate， threose acid， palmitic acid. CONCLUTION Metabonomics method can distinguish group of liver-fire hyperactivity syndrome of hypertension and group of yin-deficiency and yang-hyperactivity syndrome of hypertension. The difference of biomarker does exist in the two syndromes and is expected to provide a certain objective basis for TCM syndrome differentiation typing of hypertension.
Features of TCM Syndromes of Different Immune State with Chronic HBV Infection
YANG Li-sha, LIANG Zhi-qing, FAN Jian-wei, WU Lin-lin, SU Yuan-ying, ZHANG Bin, LIU Ling-sheng
2013, 29(4): 324-326.
[Abstract](701) [PDF 486KB](622)
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OBJECTIVE To investigate distribution characteristics of TCM syndromes of different immune state with chronic HBV infection. METHODS Immunological classification， TCM syndrome classification， and clinical epidemiology methods were applied to investigate distribution characteristics of TCM syndromes of 200 cases of immune tolerance， 200 immune clearance and 200 immunodeficiency with chronic HBV infection. RESULTS Syndromes of chronic HBV infection in three different immune status groups are liver stagnation and spleen deficiency， yin deficiency of liver and kidney， liver qi stagnation， accumulation of damp-heat， qi deficiency， yin deficiency， liver and stomach， deficiency of liver blood， lung and spleen deficiency， blood stasis， deficiency of spleen and kidney yang. The chi-square test showed P<0.05， suggesting that TCM syndrome distribution of three kinds of immune states was of statistics significance. The state of immune tolerance， immune clearance， and immunodeficiency gave the first place to liver stagnation， internal increase of dampness and heat and yin deficiency of liver and kidney， respectively. CONCLUSION TCM syndromes distribute to different immune states of HBV infection， which could provide theoretical basis for clinical diagnosis and efficacy evaluation of prevention and treatment of chronic HBV infection by the integration of TCM and western medicine.
Clinical Study of Xuzao Gengping Powder Treating Female Climactic Primary Hypertension
HE Jia, BAI Yong-jiang, GAN Qing-lei, LIN Xue
2013, 29(4): 327-330.
[Abstract](654) [PDF 312KB](635)
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OBJECTIVE To observe clinical efficacy of Xuzao Gengping Powder Treating female climacteric primary hypertension by ambulatory blood pressure. METHODS Selected 92 patients treated in Hypertension Department of Xinjiang Uygur Autonomous Region Traditional Chinese Medicine Hospital from March 2008 to October 2009. 92 cases of climacteric hypertension with internal dryness due to yin deficiency were randomly divided into 48-case treatment group and 44-case control group. 24-hour ambulatory blood pressure monitoring (ABPM) was performed before and after treatment and effect of treatment on blood pressure variability and rhythm were analyzed. RESULTS ①Compared with control group， only 24h diastolic blood pressure variation(24h DSD/DBPV) in treatment group had no statistical significance(P>0.05). 24h systolic blood pressure variation(24h SSD/SBPV)， day systolic blood pressure variation(d SSD/SBPV)， day diastolic blood pressure variation(d DSD/DBPV)， night systolic blood pressure variation(n SSD/SBPV)， night diastolic blood pressure variation(n DSD/DBPV) all had statistical significance(P<0.05). ②Compared with control group， average blood pressure expect 24h average diastolic blood pressure(24h DBP) and day average diastolic blood pressure(d DBP) in treatment group had no statistical significance(P>0.05). 24h average systolic blood pressure(24h SBP)， day average systolic blood pressure(d SBP)， night average diastolic blood pressure(n DBP)， night average systolic blood pressure(n SBP) all decreased， had statistical significance(P<0.05). As to blood pressure therapeutic effect， the total effective rate of treatment group was higher than that of control group and had statistical significance(P<0.05). Night blood pressure decrease rate ascended obviously and had statistical significance(P<0.05). CONCLUSION Xuzao Gengping Powder treating climacteric hypertension can adjust blood pressure rhyme， decrease blood variability and reduce level of systolic blood pressure obviously. Particularly， it has significant effect on night blood pressure variation.
Clinical Study of Acupoint Catgut-Embedding Therapy on Patients with Insomnia
GUO Ai-song, LI Ai-hong, FENG Lan-fang, CHEN Xin, SUN Li
2013, 29(4): 331-334.
[Abstract](692) [PDF 388KB](724)
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OBJECTIVE To evaluate the effect of acupoint catgut-embedding on patients with insomnia and to explore its mechanism. METHODS 70 cases were randomly divided into acupuncture group (35 cases) treated with acupuncture and catgut-embedding group (35 cases) treated with acupoint-catgut-embedding. Main points were Shenmen (HT7)， Sanyinjiao (SP6)， Baihui (DU20)， Anmian. Puncturing the points with filiform needle even reinforcing and even reducing in the acupuncture group， retaining the needle for 30min and stimulating the needle once every 10min， the treatment was given once a day， five times a week for 30 times totally. Catgut embedding needle and catgut were used at the points in catgut-embedding group， once 2 weeks for 3 times totally. Effect was evaluated by Pittsburgh sleep quality index scale (PSQI)， and serum cytokine Interleukin-1β (IL-1β)， Tumor necrosis factor-α(TNF-α)were detected by Enzyme-linked immunosorbent assay (ELISA) before and after treatment. RESULTS After 6 weeks' treatment， the total effective was 74.28% in acupuncture group， and that in catgut-embedding group was 91.43% (P<0.05). Scores of the factors and total scores of PSQI in both groups after treatment were decreased compared with those before treatment (P<0.05)， however， comparisons between two groups after treatment showed that the effect of catgut-embedding group was significantly superior to acupuncture group in sleep quality， daytime dysfunction， sleep time and total scores of PSQI (P<0.05). CONCLUSION Acupoint-catgut-embedding can improve the clinical effect， especially in sleep quality， daytime dysfunction， and sleep time with little adverse effects， so it is deserved to be widely applied in clinic， which maybe mechanically related to regulation of sleep-related serum cytokine .
Yangyin Runchang Decoction Treating 54 Cases of Solw Transit Constipation
YANShuai, ZENGLi, QIANHai-hua
2013, 29(4): 335-337.
[Abstract](659) [PDF 407KB](667)
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OBJECTIVE To observe clinical therapeutic effect and the short-term recurrence rate of Yangyin Runchang Decoction on slow transit constipation (STC) patients. METHODS 90 cases of STC were randomly divided into observation group (54 cases) which were treated with Yangyin Runchang Decoction and control group (36 cases) which were applied with Maren Soft Capsule. The treatment course of each group was 42 days. The clinical effect of each group was evaluated by the index of fecal character， time for defecation and intervals， difficulty of defecation， the short-term recurrence rate was also included. RESULTS After statistical analysis， the symptom scores of each group not only revealed a general trend of increase， but also indicated remarkable vary of the rises (P<0.01). The total effective rate of each group was 96% and 80% respectively. The short-term recurrence rate of two groups was 5.7% and 24.1%. CONCLUSION Yangyin Runchang Decoction can decrease the short-term recurrence incidence of STC and take an indispensable role in improving the clinical effect by reducing the symptom scores sharply.
Study on the Influence of Massage to Sciatic Nerve Injured Rat's Axoplasmic Transport Function Based on Motor Protein
YAO Bin-bin, MEI Xu-hui, WU Jian-cong, YU Tian-yuan
2013, 29(4): 338-341.
[Abstract](629) [PDF 355KB](740)
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OBJECTIVE To observe the influence of massage to the express change of sciatic nerve injured rat's axoplasmic transporting motor protein. To investigate the effect mechanism of massage treating sciatic nerve injury.METHODS A sciatic nerve clamping rat model was set. The pathological and behavioral changes of rats in normal group， sham operation group，model group， model control group and massage group were observed through measuring the wet weight of the gastrocnemius and heat pain threshold experiment. Kinesin in rats' spinal cord and dynein express situation were observed by immunohistochemical staining and the differences between each group were counted.RESULTS The rat's sense and movement function decreased obviously after its sciatic nerve was injured according to the heat pain threshold and wet weight of the gastrocnemius tests of rats in the model group and model control group. After the massage treatment， the experimental evaluation of wet weight of the gastrocnemius test and heat pain threshold experiment increase significantly and there were no significant difference as compared with the normal group 20 days after treatment. The kinesin and dynein immunohistochemical expression of the model group， control group and massage group obviously increased compared with the normal group. There were significant differences among massage group， model group and control group.CONCLUSION The massage treatment can promote the recovery of axoplasmic transporting function by increasing the expression of axoplasmic transporting motor protein kinesin and dynein. Then the neuronal cells and nerve innervation targeted tissue are two-way influenced. Finally， massage treatment can improve the sense and movement function of sciatic nerve injured rat.
Effects of Liuwei Dihuang Decoction on Expression of HIF-1α and CTGF in Kidney Tissues of Chronic Renal Failure Rats
TANGQun, HEZe-yun, CHENLi, XUWen-feng, ZENGHai-fei
2013, 29(4): 342-346.
[Abstract](646) [PDF 1753KB](640)
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OBJECTIVE To determine the expressions and effects of HIF-1α and CTGF in kidney tissues of chronic renal failure rats， and to observe the influence of Liuwei Dihuang Decoction on the expression.METHODS The rat models of chronic renal failure were induced by excising 5/6 kidney， which were divided into sham operation group， model group and Liuwei Dihuang Decoction group. After 12 weeks， each index of renal functions was detected. HE was stained to observe renal pathological changes and Masson to observe the deposition of renal tissue collagen; immunohistochemical method and RT-PCR were applied to detect protein levels and gene expression of HIF-1α and CTGF in renal tissues respectively.RESULTS Compared with sham operation group， the protein level and gene expression of HIF-1α and CTGF in renal tissues in model group were significantly elevated(P <0.01). Compared with model group， the protein level and gene expression of HIF-1α and CTGF in renal tissues in treatment group were significantly reduced(P<0.01).CONCLUSION HIF-1α can promote renal interstitial fibrosis by up-regulating CTGF expression while Liuwei Dihuang decoction may attenuate renal interstitial fibrosis by reducing HIF-1α expression.
Influence of Tongxie Yao Fang on ERK1 and ERK2 Gene Expressions of Colonic Mucosa in Rats with Ulcerative Colitis
ZHU Xiang-dong, DUAN Yong-Qiang, WANG Yan, WANG Bin, CAO Yan-fei
2013, 29(4): 347-350.
[Abstract](634) [PDF 3321KB](637)
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OBJECTIVE To observe the influence of Tongxie Yao Fang on the ERK1 and ERK2 gene expressions of colonic mucosa in rats with ulcerative colitis （UC）， and to identify its underlying mechanism of action.METHODS Experimental animals were divided into six groups and prepared by means of TNBS/ ethanol enema to observe their morphological injury and damage with the naked eyes， and to detect ERK1 and ERK2 gene expressions by means of RT-PCR with ERK1 and ERK2 being the observation targets.RESULTS Inflammation and ulceration on the colonic mucous membrane of rats were observed with naked eyes， which suggested a successful modeling compared with the control group (P<0.05). The relative expression amount of ERK1 and ERK2 of colonic tissues of rats in the model group was higher than that of the blank group （P<0.05）; after treatment， compared with the model group， the relative expression amount of ERK1 and ERK1 in the prescription high- and moderate-dose groups were increased （P<0.05 and P<0.01）.CONCLUSION Tongxie Yao Fang can promote the relative expression amount of ERK1 and ERK2 of colonic tissues of rats with UC， which suggests the therapeutic effects of the prescription are probably related to the activation of ERK signal transduction pathway.
The Inhibitory Effect of Toxic Chinese Drugs on the Growth of Human Colon Cancer Cell Line HCT116 and SW480
ZHUPing, GUYun-fei, YANGBo-lin, LINQiu, CHENHong-jin, SUNGui-dong, DINGYi-jiang
2013, 29(4): 351-354.
[Abstract](655) [PDF 551KB](644)
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OBJECTIVE To screen out the medicines that can be specifically against human colon cancer cells from the already known toxic antineoplastic Chinese drugs.METHODS Oldenlandia diffusa， Tripterygium wilfordii， Paris polyphylla， Solanum nigrum， Gamboges， Rhizoma arisaematis， Dioscorea bulbifera L， Cinobufacini， Sodium cantharidinate and Sodium arsenic were applied to treat the human colon cancer cell line HCT116(MLH1-deficient) and SW480(proficient mismatch repair)， MTT were used to analyze the growth inhibition of two cell lines.RESULTS Sodium cantharidinate， Cinobufacini， Sodium arsenic and Gamboges can inhibit cell growth of HCT116 and SW480， The IC50 values of HCT116 cell line were significantly lower than SW480 cell line when they were both treated with Sodium cantharidinate Cinobufacini and Gamboges(P<0.05). Meanwhile， there were no significant difference between the IC50 values of HCT116 cell line and SW480 cell line when they were treated with Sodium arsenic(P>0.05). However， Oldenlandia diffusa， Tripterygium wilfordii， Paris polyphylla， Solanum nigrum， Rhizoma arisaematis， Dioscorea bulbifera L did not present growth inhibition effect on HCT116 and SW480 in this study.CONCLUSION Gamboges， Sodium cantharidinate， Sodium arsenic and Cinobufacini can effectively inhibit the growth of human colon cancer cells developing from different genetic pathways. Gamboges， Sodium cantharidinate and Cinobufacini may specifically against MMR-deficient human colon cancer cells.
Proliferation and Mechanism of bEnd.3 Induced by Low Dose FasL
ZHANGChun-bin, ZHANGMin-shun, TENGFeng-meng, GAOFeng
2013, 29(4): 355-358.
[Abstract](646) [PDF 2107KB](683)
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OBJECTIVE To investigate the proliferation and possible mechanism of rat's cerebral microvascular endothelial cells induced by low dose FasL.METHODS 9 FasL of different concentration gradients was diluted 500~0.015 6 ng/mL 1/2 multiple proportion and the cell culture of bEnd.3 was interfered by 48 h. The cell proliferation ability of bEnd.3 was tested by CCK-8. The VEGF cell secreted expression ability of bEnd.3 was tested by ELISA. Fas Expression was inhibited by RNAi. The protein express levels of FADD， FLIP and TRAF were tested by Westernblot， and the protein express level of NF-κB was tested by EMSA.RESULTS bEnd.3 cell interfered by 0.156 ng/mLFasL could obviously induce the cell proliferation to increase(P<0.05). VEGF cell secreted expression ability of bEnd.3 was markedly increased(P<0.05). The protein express levels of FADD，FLIP，TRAF and NF-κB increased significantly(P<0.05); The cell proliferation and protein express levels of NF-κB did not change significantly although Fas gene was inhibited by RNAi(P>0.05). The protein express levels of FADD， FLIP， TRAF decreased obviously when Fas gene was inhibited by RNAi(P<0.05). CONCLUSION Low dose FasL can induce the cell proliferation of bEnd.3， and FADD-FLIP-TRAF-NF-κB signal transduction pathway is one of its mechanisms.
Effects of Xuemaitong Granule on Expression of Matrix Metalloproteinase(MMP) and Tissue Inhibitors(TIMP) of Carotid Atherosclerosis
ZHANG Qi, QIAN Gao-chao, DING Zhi-xiang
2013, 29(4): 359-361.
[Abstract](625) [PDF 391KB](638)
Abstract:
OBJECTIVE To investigate the effects of Xuemaitong Granules on expression of matrix metalloproteinases(MMPs) and matrix metalloproteinase inhibitors (TIMPs) in apoE-deficient mice.METHODS Male ApoE-deficient mice (n=33， aged 8 weeks， 18 to 20 g) on a C57BL/6J background， fed a high fat diet for 13 weeks. After confirming the atherosclerosis plaque formed， mice randomly divided into three groups: Xuemaitong Granules group， lipitor group and control group. After six weeks， venous blood and aorta were taken. The expression of MMP-2， MMP-9， TIMP-1 and TIMP-2 were measured by ELISA， Q-PCR and Werstern blot， respectively.RESULTS Compared with control group， the serum concentration of MMP2 and MMP9 significantly decreased (P<0.05) in two intervention groups and there was no significant difference(P>0.05) in them. The serum concentration of TIMP1 in Xuemaitong granules intervention group is much higher than in the other groups. However， The serum concentration of TIMP2 in the lipitor intervention group is much higher than that in the other groups. The same results were also detected by Q-PCR and Western blot in the aortic plaques.CONCLUSION Xuemaitong Granules can reduce the expression of MMP2 and MMP9 and up-regulate the expression of TIMP-1 in apoE-deficient mice.
Proliferative inhibition effects of Lingba Junzhi alkaline constituents on human non-small cell lung cancer A549 cells and its mechanism
SUN Yu, PENG Wei, PAN Yang, ZHANG Xian, JIANG Ya-ping
2013, 29(4): 362-367.
[Abstract](630) [PDF 945KB](747)
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OBJECTIVE To explore the proliferative inhibition effects of Lingba Junzhi alkaline constituents on human non-small cell lung cancer A549 cells and its mechanism via in vitro cell culture.METHODS The alkaline constituents were obtained by alkali-solution and acid-isolation extraction， and solvent extraction of Lingba Junzhi methyl alcohol extract parts; HPLC was applied to verify the existence of Crontonside. MTT assay， soft agaragar assay， PT single-stain and immunofluorescent staining were later used to observe its effects on inhibiting A549 cell proliferation， tumor formation， growth cycle distribution and cytoskeleton destruction.RESULTS Crontonside did exist in Lingba Junzhi alkaline constituents， which significantly inhibited A549 proliferation and reduced cell clone formation， delayed the cell circle in G2 period and caused certain damage to the cytoskeleton.CONCLUSION Lingba Junzhi alkaline constituents can inhibit in vitro A549 cell proliferation and its mechanism may be related to its decreasing clone formation ability and damaging cytoskeleton.
Study on Preventing Rat Atherosclerosis with Jiangzhi Pills
SHENG Yi-liang, SHEN Jian-fei, GAO Hong-qin, SHEN Yi-xin, GAO Wen-yuan, QIAN Jian-ming, XU Dan, XU Qian
2013, 29(4): 368-371.
[Abstract](601) [PDF 3254KB](705)
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OBJECTIVE To observe the effect of prophylactic medication Jiangzhi Pill on blood fat of atherosclerosis rats (atherosclerosis， AS) and morphological changes of arcus aortae. METHODS 72 SD rats were randomly divided into six groups: (1)Jiangzhi Pill high dose group， (2)Jiangzhi Pill middle dose group， (3)Jiangzhi Pill low dose group， (4)gynostemma pentaphylla intervention group， (5)blank group and (6)model group. The normal diet was given to the blank group and the model group was given lipid emulsion gavage. The other groups were given corresponding drug treatment while modeling. Measure the triglycerides (TC)， low density lipoprotein (LDL-C) and C-reactive protein (CRP) respectively on D15， 25， 34. Lastly， the arcus aortae and liver pathology morphology was observed.RESULTS The TC， LDL-C in Jiangzhi Pill of the high， middle and low dose groups after drug administration of 15 days， 25days， 34days were significantly lower than the model group (P<0.01)， better than gynostemma pentaphylla. CRP in Jiangzhi Pill of the high， middle and low dose groups was significantly lower than those of the model group (P<0.01) and the effect of high dose group was better than gynostemma pentaphylla and the middle and low dose groups were similar to gynostemma pentaphylla. The rat weight given lipid emulsion gavage was significantly lighter. Liver index in Jiangzhi Pill of the high， middle dose groups， compared with model group， were significantly different (P<0.01). Liver fat degree: (6)>(4)>(3)=(2)>(1)>(5). The hardening situation of arcus aortae: (6)>(2)=(3)>(4)>(1)>(5).CONCLUSION Jiangzhi Pill have a antihyperlipidemic effect and can effectively prevent the occurrence of AS and fatty liver.
Research on An LC-MS Method for the Simultaneous Determination of Baicalin， Chlorogenic Acid and Forsythiaside A in Rat Plasma and its Application to Pharmacokinetic Study of Shuang-huang-lian in Rats
HANXue-bing, TIANLe, ZHOUWei, DILiu-qing
2013, 29(4): 372-375.
[Abstract](878) [PDF 2411KB](643)
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OBJECTIVE To establish the simultaneous determination of baicalin， chlorogenic acid and forsythiaside A in rat plasma and its application to pharmacokinetic study of Shuang-huang-lian in rats.METHODS Plasma samples were prepared with methanol-ethye acetate-trifluoroacetic acid (49∶48∶3) at a flow rate of 1.0 mL/min and detected with a mobile phase of acetonitrile-water containing 0.2% formic acid using ESI with negative ionization mode. Ions monitored in SIM mode were set as m/z 353[M-H]- for chlorogenic acid; m/z 445[M-H]- for baicalin; m/z 623[M-H]- for forsythiaside A. The concentration-time curves of baicalin， chlorogenic acid and forsythiaside A were simulated by Winnonlin5.2 and the pharmacokinetic parameters were calculated.RESULTS The calibration curve was linear within the range of 10~2 000 ng/mL for baicalin， 5~500 ng/mL for chlorogenic acid and forsythiaside A (r≥0.991). The RSDs of intra and inter day were all less than 15%， and the stability of baicalin， chlorogenic acid and forsythiaside A were controlled by the range of 90.9%~103.1%. The methods were all satisfied for the FDA level in analysis drug in vivo.CONCLUSION This method is simple， sensitive， and accurate， which can be used for analyzing Shuang-huang-lian in vivo.
Study on HPLC Fingerprint of Oxytropis falcata
YANKe, GUQing, YANGGuang-ming, CHENXing, CAIBao-chang
2013, 29(4): 376-378.
[Abstract](687) [PDF 493KB](642)
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OBJECTIVE To establish fingerprint chromatograms of Oxytropis falcata by HPLC.METHODS Analysis was performed on a Kromasil C18 (250 mm×4.6 mm， 5 μm) column with methanol-0.1%phosphoric acid water solution as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was set at 270 nm and the column temperature was 30 ℃.RESULTS The similarities of the eleven batches of O. falcata from different origin were calculated over 0.9 by analyzing eight common peaks in the fingerprint.CONCLUSION This method is repeatable， accurate， precise and stable and can be used for identification and evaluation of O. falcata.
Analysis of Chemical Components of Volatile Oil from Cardamine Urbaniana by GC-MS
RUANXiao-yun, FUTing-xiong, LUJin-qing
2013, 29(4): 379-381.
[Abstract](636) [PDF 736KB](667)
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OBJECTIVE To analyze the chemical components of volatile oil from Cardamine Urbaniana by GC-MS.METHODS Volatile oil was extracted by steam distillation.The chemical components of volatile oil were analyzed by GC-MS.RESULTS 35 compounds were identified. The main component is 4-isothiocyanato-1-Butene(65.71%).CONCLUSION As the first time to analyze the chemical components of volatile oil from Cardamine Urbaniana by GC-MS， this study can provide scientific basis for further research and development of Cardamine Urbaniana.
Analysis on Chemical Components from Water Extract of Ligusticum Chuanxiong by UPLC-ESI-Q-TOF-MS
XUXiao-fang, SUNDong-dong, LIXiang, CHENJian-wei, CuiJiu-cheng
2013, 29(4): 382-386.
[Abstract](712) [PDF 455KB](943)
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OBJECTIVE To make a qualitative analysis on chemical components in water extract of Ligusticum chuanxiong by UPLC-ESI-Q-TOF-MS.METHODS LC-MS/MS， C18(4.6 mm×250 mm，5 μm) column was adopted，with methanol -0.1% formic acid as the mobile phase for gradient elution; ESI ion source was used for mass spectra，and data were collected in positive and negative mode.RESULTS By analyzing positive and negative ion mass spectra information， element composition as well as comparing controls with data from relevant literature， thirteen compounds from water extract of Ligusticum chuanxiong Hort were identified. CONCLUSION After the separation by ultra high performance liquid chromatography， relative molecular mass was determined by mass spectra， and chemical compounds in water extract of Ligusticum chuanxiong Hort were determined by information search through relevant literature data， which served as a powerful proof for pharmacodynamic material basis of water extract from Ligusticum chuanxiong.
Study of Deficiency and Excess Syndrome of Body Fluid Metabolism in Sepsis Patients
ZHOU Sui-feng, MIAO Ying-nian
2013, 29(4): 387-389.
[Abstract](602) [PDF 2042KB](669)
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VE: OBJECTIVE To discuss clinical characteristics of body fluid of metabolism septic patients of deficiency and excess syndrome in ER. METHODS The characteristics were compared between two groups of septic patients in ER from January 2009 and October 2011. RESULTS There were no significant differences in the patient age， mortality， hemodynamic changes and the length of stay between two groups. Nevertheless， there were remarkable differences in the rate of respiratory machine， input and output of liquid and the APACHE II score. CONCLUSION The fluid collection is easy to appear in deficiency syndrome patients. The effective circulating blood volume is not enough in excess syndrome patients. Fluid therapy can achieve good outcome in excess syndrome patients， but not in deficiency syndrome patients.
Effects of Acupuncture and Acupoint Bloodletting on T Cell Subsets in Patients with Eczema
XU Jia, XIE Qian
2013, 29(4): 390-392.
[Abstract](662) [PDF 341KB](697)
Abstract:
OBJECTIVE To observe the differences of curative effects between acupuncture and acupoint bloodletting and western medicine and to explore the effects on T cell subsets(CD4+，CD8+，CD4+/CD8+) in patients with eczema of acupuncture and acupoint bloodletting. METHODS According to visiting sequence， 62 patients with eczema were randomly divided into acupuncture and acupoint bloodletting group(treatment group) and western medicine group(the control group). Treatment group was divided into three types in the method of dialectical classification with acupuncture and acupoint bloodletting therapy while control group taken Cetirizine Hydrochloride orally. After 6 weeks' treatment， the clinical curative effects of the two groups were observed. In addition， T cell subsets levels in the treatment group were tested before and after treatment. RESULTS The total effective rates of treatment group and control group were 83.9% and 58.1% respectively， the former being better than the latter(P<0.05). CD4+ level in treatment group increased after treatment(P<0.01)， CD8+ decreased(P<0.01) and CD4+ / CD8+ increased(P<0.01). CONCLUSION The western medicine is obviously inferior to acupuncture and acupoint bloodletting therapy which can regulate T cell subsets levels in eczema patients' blood and help recover the immune stability of human body.
Updated Pathogenesis of Hepatic Injury in Severe Acute Pancreatitis and the Treatment of Traditional Chinese Medicine
WULi, CAIBao-Chang
2013, 29(4): 393-396.
[Abstract](747) [PDF 351KB](707)
Abstract:
Antitumor Research and Clinical Applications of Bitter Leaf
YANG Zao
2013, 29(4): 397-400.
[Abstract](912) [PDF 410KB](798)
Abstract:

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