2013 Vol. 29, No. 3

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On Eight Principles and Two Principles-Six Essentials
ZHANG Yu
2013, 29(3): 201-204.
[Abstract](993) [PDF 382KB](820)
Abstract:
Based on the detailed description of the origin， development and maturity of "Eight Principles"， this paper pointed that ignoring the logic defects on equally treating the importance of "Eight Principles" yin， yang， exterior， interior， cold， heat， deficiency and excess syndromes would lead to students' confused understanding of the concept of "yin and yang syndrome differentiation" as well as its connotation. It is suggested that the recapitulative and governing status of yin and yang should be emphasized. That is to say， "Two Principles" and "Six Essentials" should be discussed respectively under the chapter named "Eight Principles Syndrome Differentiation" so as to ensure the completeness of "Eight Principles"， stress the recapitulative and governing status of yin and yang， and fully reflect the hierarchy of "Two Principles" and "Six Essentials".
Recording Characteristics and Thoughts of Anonymous Prescriptions in Arcane Essentials from the Imperial Library
WU Cheng-yan, JI Dan-dan
2013, 29(3): 205-207.
[Abstract](988) [PDF 357KB](777)
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By the collection and study of the prescription masterpiece Arcane Essentials from the Imperial Library in Tang Dynasty， this research aims at discussing the recording characteristics and thoughts of this book and considers that there hides large amount of anonymous prescriptions in prescription literatures， which are not only reflecting the traditional and plain thoughts of previous doctors， more important things are the crystallization of both authentic records and clinical experience collected from clinical doctors treating and drug-using. This is an abundant resource which is worth studying and developing for utilizing.
On the Correlation Between the Features of Disease Location and Nature and the Basic Syndromes of the Heart System by Fuzzy Mathematics
YANG Tao, WU Cheng-yu
2013, 29(3): 208-210.
[Abstract](901) [PDF 341KB](694)
Abstract:
To discuss the correlation between the features of disease location and nature and the basic syndromes of the heart system by fuzzy mathematics. According to five-zang syndrome differentiation system together with "principle of proximity" in fuzzy recognition， MATLAB was used to set up the fuzzy recognition model of the basic syndrome of the heart system to test information about 1741 cases and analyze the correlation in model. The features of disease location and nature and the basic syndrome of the heart system existed fuzzy mapping relation. Most of the correlation can well reflected the features of disease location and nature of the heart system while individual correlation suggested features of mingling disease location and nature. Fuzzy mapping relation between the features of disease location and nature and the basic syndrome of the heart system is imitated effectively by fuzzy mathematics， which can be applied to five-zang syndrome differentiation system and provide a reference for the study of diseases syndrome.
Clinical Research of Cotton-sheet Moxibustion for Treating Herpes Zoster
ZUOJia, HEJia, DUChen, YANGYun-kuan
2013, 29(3): 211-213.
[Abstract](1128) [PDF 353KB](760)
Abstract:
OBJECTIVE To evaluate the clinical effects of cotton-sheet moxibustion therapy and western medicine therapy for the treatment of herpes zoster. METHODS 201 cases with herpes zoster received and cured in 9 institutes in Chengdu， Wuhan， Guangzhou from January of 2008 to June of 2009 were randomized into cotton-sheet moxibustion group (99 cases) and western medicine group (102 cases). The cotton-sheet moxibustion group were given cotton-sheet moxibustion supplemented by acupuncture treatment. The western medicine group were given regular western medicine. RESULTS ①The total effective rate of cotton-sheet moxibustion group was 92.2% and western medicine group 91.2%. There was no statistical difference (P>0.05); ②In the aspects of incrustation time and decrustation time， there was statistical significance when the cotton-sheet moxibustion group were compared with western medicine group (P<0.01); ③As for the duration of pain lasting， there was significant statistical difference between the two groups (P<0.01); ④As for the occurrence of postherpetic neuralgia， there was significant statistical difference between the two groups on the 30th day and 60th day. (P<0.05~0.01). CONCLUSION The cotton-sheet moxibustion therapy has accurate effects on herpes zoster， which has no obvious difference with western medicine regular therapy. In the aspects of promoting incrustation， shortening incrustation time and pain duration and controlling postherpetic neuralgia of herpes zoster， the cotton-sheet moxibustion therapy is better than the western medicine therapy.
Clinical Observation on Trigeminal Neuralgia in Different Moxibustion Positions Treated by Thermal Moxibustion
FUYong, ZHANGHai-feng, XIONGJun, ZHANGBo, LILin, CHENRi-xin
2013, 29(3): 214-216.
[Abstract](1094) [PDF 303KB](667)
Abstract:
OBJECTIVE To compare the therapeutic effects of Thermal Moxibustion in the treatment of primary trigeminal neuralgia in different moxibustion positions. METHODS 62 cases of primary trigeminal neuralgia were randomly divided into a treatment group (32 cases) treated with mild and suspended moxibustion on thermal acupoint， and a control group (30 cases) treated with mild and suspended moxibustion on non-thermal acupoints. 30 days made up the course of treatment (40min each time， and two times per day in the first 5 days and 1 time per day in the following 25 days). The changes of therapeutic effects， visual analogue scale (VAS)， and Migraine specific quality of life questionnaire (MSQ) of the two groups were contrasted and evaluated after the treatment. RESULTS Compared with pre-treatment， the treatment group had a remarkable difference (P<0.05~0.01) in VAS， MSQ score. In contrast to the control group， there was statistically significant difference in VAS score in treatment group(P<0.05)， with more significant difference in MSQ score(functional limitations， dysfunction， emotion)(P<0.05~0.01). The total effective rate of the treatment group was 78.13%， better than 53.33% of the control group(P<0.05). CONCLUSION Suspended moxibustion on thermal acupoint， which is superior to suspended moxibustion on non-thermal acupoint in the treatment of primary trigeminal neuralgia， can improve the therapeutic effects of moxibustion.
Clinical Research on Distal Radius Fracture Treated by Splint Support of Non-invasive and Regulating Type
YANSong-he, HUGang, CAIJian-ping, LIUXin-huan
2013, 29(3): 217-219.
[Abstract](955) [PDF 344KB](747)
Abstract:
OBJECTIVE To compare and analyze the effects of splint support of non-invasive and regulating type with splint of plastic lead wire paper type on local anatomy and wrist joints function. METHODS 40 patients with distal radius fracture received and cured in our department from January to December in 2012 were randomized into two groups: splint support of non-invasive and regulating type group (20 cases) and splint of plastic lead wire paper type group (20 cases). No statistical difference existed in the age， sex and fracture type of two groups. RESULTS After all patients received follow-ups， it was discovered that all fractures were healed without any complication. Based on statistical analysis， there was no difference among the recovery of local anatomy of distal radius， maintaining of palmar tilt and ulna deflection angle in both groups (P>0.05). But splint support of non-invasive and regulating type group had better effects than the splint of plastic lead wire paper type group on the maintaining of radial height and recovery of wrist joints function (P<0.05). CONCLUSION Splint support of non-invasive and regulating type has good effects of healing distal radius fracture， maintaining the anatomy of distal radius， avoiding the shortening and deformity and restoring the wrist joints function.
90 Cases of Plasma Cell Mastitis Treated by Combination of Chinese and Western Medicine
NiYi, YiFei, XueBo-yu
2013, 29(3): 220-222.
[Abstract](919) [PDF 1318KB](651)
Abstract:
OBJECTIVE To compare the clinical effect of Chinese herbs， Fibroptic Ductoscopy and combination of both in the treatment of Plasma Cell Mastitis. METHOD 90 cases were equally grouped into A group treated with Chinese herbs， B group treated with Fibroptic Ductoscopy and C group treated with Chinese herbs plus Fibroptic Ductoscopy. RESULTS The curative rate of C group was 70.0% (P<0.05)， which was superior to the other two groups. The effective rate of C group was also better than A and B group， reaching 86.7% (P<0.05). Average course of the treatment group was (4.95±2.16) weeks， significantly shorter than the other two groups. CONCLUSION Chinese herbs plus Fibroptic Ductoscopy or combination of Chinese and western medicine， which has higher curative rate as well as shorter course of treatment， is an effective treatment for Plasma Cell Mastitis.
Self-made Xianxiong Mixture and Beiou Cataplasm Treating Reflux Esophagitis: 100 cases
ZHANG Hong-wei, SHEN Zu-fa, LU Jian-yun, WENG Chun-mei, XU Wei, JIN Jing
2013, 29(3): 223-226.
[Abstract](1636) [PDF 394KB](635)
Abstract:
OBJECTIVE To observe the clinical effects of Xianxiong Mixture and Beiou Cataplasm for treating reflux esophagitis (RE). METHODS 200 patients were randomized into treatment group (100 cases treated by Xianxiong Mixture and Beiou Cataplasm) and control group (100 cases treated by omeprazole). The treatment course for both groups are 8 weeks. The effects and integral changes of clinical symptoms， effects of gastroscope， RE grouping and integral evaluation under gastroscope， and relapse were compared before and after the treatment. RESULTS The total effective rate of the treatment group is 93% and control group 84%. There was significant difference between the two groups (P<0.05). The integral and total integral of each symptom in both groups all decreased. Compared with the condition before the treatment group， there was significant difference (P<0.05). After the treatment， significant difference exists in the symptom improvement of burning heart， reflux， retrosternal pain but no significant difference exists in the symptom improvement of dysphagia. The total effective rate of treatment group is 93% and control group 84% by gastroscope reexamination after 8 weeks. There was significant difference between the two groups (P<0.05). The gastroscope RE grading in both groups declined after the treatment. After administration of 6 months， the recurrence rate of treatment group is 5.56% and control group 30.43% and there was significant difference (P<0.05). CONCLUSION The therapeutic effects of Xianxiong Mixture and Beiou Cataplasm for treating RE is better than that of the control group. At the meanwhile， it has low recurrence rate and can enhance the life quality of the patients.
Effect of Radix Astragali on TGF-β1/Smads Signaling Pathway in Epithelial-mesenchymal Transition of rat Peritoneal Mesothelial Cells Induced by High-glucose Peritoneal Dialysate
LIZheng-hong, SHENGMei-xiao, ZHANGXu, ZHANGLu, ZHUChang-le, YANGJin-song
2013, 29(3): 227-232.
[Abstract](982) [PDF 1436KB](635)
Abstract:
OBJECTIVE To observe the effect of Radix Astragali on TGF-β1/Smads signaling pathway in epithelial-mesenchymal transition(EMT) of rat peritoneal mesothelial cells induced by high-glucose peritoneal dialysis and explore the inhibitory influence and mechanism of Radix Astragali on peritoneal fibrosis.METHODS They were divided into 2 groups， 10 rats in each. The contrast group's rats were intraperitoneal injected 35days normal saline 20 mL/rat. The model group's rats were intraperitoneal injected 35days Icodextrin 20 mL/rats. The low/middle and high doses astragalus mongholicus groups: After 16 days modeling， the rats were intraperitoneal injected 20 days astragalus mongholicus extraneal(put astragalus mongholicus injections into the externeal， and the concentation of crude drug is 10 mg/mL，20 mg/mL and 40 mg/mL) 20 mL/rats. The peritoneal pathological changes were observed through HE staining; the expressions of abdominal membrane TGF-β1， Collagen I， Collagen III， FN， E-cadherin， α-SMA were examined through rapid immunohistochemical method; the expressions of proteins of peritoneal tissues TGF-β1， E-cadherin， α-SMA， Smad2/3， p-smad2/3， of Smad7 were detected through Westernblot; the expressions of TGF-β1， E-cadherin， α-SMA， Smad7mRNA were detected through RT-PCR.RESULTS ①PMCs in model group were round or oval with cells falling off， subcutaneous matrix proliferating， a large number of fibroblast-like cells and monocytes and macrophage infiltrating， fibrinoid material depositing and the expression of peritoneal tissues TGF-β1， Collagen I， Collagen III and FN increasing. The astragalus intervention groups showed improvement in the above conditions with the high-dose group the most obvious. ②The expression of α -SMA in model group labeled by EMT showed an up-regulation while that of E-cadherin low-regulation. The middle and high doses of Radix Astragali groups could reverse the decrease in expression of E-cadherin in peritoneal tissues and high expression of α-SMA to some extent. ③In model group， the expression of signal proteins p-Smad2/3 and Smad7 showed an up-regulation. Radix Astragali could decease the high expression of p-Smad2/3 in different degrees and strengthen the expression of Samd7 taking the high-dose group as the more significant effect.CONCLUSION High-glucose abdominal dialysis fluid can prompt the occurrence of PMCsEMT while Radix Astragali may inhibit TGF-β1 and effect the key signal proteins Smad2/3 and Smad7 in the positive and negative feedback loop of Smads signal transduction pathways to prevent the occurrence of PMCsEMT.
Effects of Zuogui Pill Medicated Serum on the Expressions SPARC during the Differentiation of Rat Marrow Mesenchymal Stem Cells towards Chondrocytes
GANKe, XULing-xiao, WANGFang, ZHANGMiu-jia, TANWen-feng, ZHANGQian-de
2013, 29(3): 233-237.
[Abstract](952) [PDF 505KB](684)
Abstract:
OBJECTIVE To investigate the effects of Zuogui Pill (ZP) medicated serum on the expressions SPARC during the differentiation of rat marrow mesenchymal stem cells towards chondrocytes.METHODS Mesenchymal stem cells (MSCs) isolated from rat bone-marrow were induced differentiation towards chondrocytes and stimulated with ZP in the meantime. The impact of Zuogui Pill medicated serum on the expression of SPARC mRNA around the induced 0， 7， 14 and 21d respectively was assayed by Real-time PCR. SPARC promoter reporter vector was constructed and transfected into bone marrow mesenchymal cell line. After cells treated with or without ZP for 48h， cell lysates were prepared and assayed for promoter activity using the Dual-Luciferase reporter assay.RESULTS The expression of the SPARC mRNA was significant increased in ZP groups than those in blank or non-ZP group on the induced 21 day (P<0.05). The promoter activity of SPARC displayed a one-fold increasing after ZP stimulation compared to blank group (P<0.05).CONCLUSION Zuogui Pill medicated serum can promote SPARC mRNA expression during the differentiation of rat MSCs to chondrocytes mainly through enhancing SPARC gene transcription activity.
Methanol Extract of Zizyphi Spinosi Semen Increases the REM Sleep of Daytime Associated Modulation Mechanism
HU Zhen-zhen, CAI Zhen-yu, ZHU Jun, XU Fang-yun, WANG Hong-mei, YAN Min
2013, 29(3): 238-242.
[Abstract](976) [PDF 948KB](678)
Abstract:
OBJECTIVE To investigate whether methanol extract of Zizyphi Spinosi Semen (MEZSS) has hypnotic effects through the γ-aminobutyric acid (GABA) ergic systems.METHODS The hypnotic effects of MEZSS were measured with electroencephalogram (EEG) activity in rats. Additionally， the chloride influx was estimated in primary cultured cerebellar granule cells with treatments of MEZSS or pentobarbital. On 1st day of treatments of MEZSS and diazepam， the GABAA receptor subunits and glutamic acid decarboxylase (GAD) expression was determined in hypothalamus in rat and cerebellar granule cells.RESULTS During daytime， MEZSS increased the rapid eyemove (REM) sleep and the EEG θ-wave activity. In addition， both of MEZSS and pentobarbital increased chloride influx. MEZSS significantly increased GABAA receptors γ-subunit expression and had no effect on the expression of α- and β- subunits. In addition， MEZSS enhanced expression of GAD， but pentobarbital did not.CONCLUSION SMEZSS increases the REM sleep behaviors through the modification of GABAergic systems.
The Protective Effect of Radix Rehmanniae and Cornus Officinalis on HUVEC Stimulated by AGEs
WUJia-lei, XUHui-qin, TANZhen, LILi, TAOYu-han, LIWei
2013, 29(3): 243-246.
[Abstract](989) [PDF 897KB](685)
Abstract:
OBJECTIVE To detect the protective effect of Radix Rehmanniae and Cornus Officinalis on HUVEC stimulated by advanced glycation endoproducts (AGEs).METHODS The cultured HUVEC cells were randomly divided into normal， AGEs， Radix Rehmanniae， Cornus Officinalis， Radix Rehmanniae-Cornus Officinalis separated and combined decoction groups. Firstly， adding 0.125 g/L，0.062 5 g/L，0.031 25 g/L final concentration drugs in advance for 1 h. Secondly， using 200 mg/L AGEs as stimulus to continue culturing for 24 h. Thirdly， using CCK-8 to measure and calculate the protection rate of drugs on HUVEC cells stimulated by AGEs. Last， measuring SOD， MDA， NO， LDH， TNF-α， IL-1β by ELISA method. RESULTS Different density of Radix Rehmanniae， Cornus Officinalis， and Radix Rehmanniae combining Cornus Officinalis all have protective effect on HUVEC cells stimulated by AGEs. The SOD， NO decreased (P<0.05~0.01)， MDA， LDH， TNF-α， IL-1β increased (P<0.05~0.01) in the supernate of AGEs group. Medication administration groups can increase SOD， NO， decrease MDA， LDH， TNF-α， IL-1β， and have a significant difference compared to AGEs group (P<0.05~0.01).CONCLUSION Radix Rehmanniae， Cornus Officinalis， and Radix Rehmanniae combining Cornus Officinalis can protect vascular endothelial cells by increasing production of NO， inhibiting oxidative stress， and regulating emiocytosis. Especially the Radix Rehmanniae combining Cornus Officinalis group has a more obvious effect.
Effect of Anti-heart-failure Granule on RAAS and TGF-β1 in of Rats with Diastolic Heart Failure
MIAOMeng-lu, LIQi-yi, YANShi-hai, ZHUBo, DAIHai-yun
2013, 29(3): 247-250.
[Abstract](901) [PDF 364KB](699)
Abstract:
OBJECTIVE To observe the effect of anti-heart-failure granule on plasma renin activity(PRA)， angiotensinⅡ(AT-Ⅱ)， aldosterone (ALD) and transforming growth factorβ1(TGF-β1) of rats with diastolic heart failure. METHODS With Abdominal aorta constriction to establish model of diastolic heart failure rat， rats were randomly divided into six groups， namely model group， benazepril group， large， medium and small dose of anti-heart-failure granule groups as well as sham-operation group. PRA， AT-Ⅱand ALD of the rats in different groups were tested. The expression of TGF-β1 in cardiomyocytes was carefully detected respectively by immunofluorescence staining techniques and Western-Blot analysis.RESULTS Compared with the model group， AT-Ⅱof benazepril group and anti-heart-failure Granule large dose group decreased(P<0.05); ALD of benazepril goup and anti-heart-faiure Granule large， medium groups decreased(P<0.05). After detection by immunofluorescence staining techniques， it indicated that TGF-β1 of Benazepril group and Anti-heart-failure Granules groups were lower than model group(P<0.05)， while after Western-Blot analysis， it showed that the expression of TGF-β1 of Benazepril group and Anti-heart-failure Granules large dose group was suppressed compared with the model group (P<0.05).CONCLUSION Anti-heart-failure granule may reduce AT-Ⅱand ALD of rats with diastolic heart failure and also may suppress the expression of TGF-β1 in cardiomyocytes.
The Effects of Chuangliye decoction loading into modified collagen matrices on angiogenesis
ZHANGYu-xuan, YAOChang, ZHUYong-kang, ZHAOHui, HEPeng, CAODong-yang, GUOMeng-meng
2013, 29(3): 251-254.
[Abstract](921) [PDF 628KB](671)
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OBJECTIVE To investigate the effects of Chuangling liquid and its loaded Collagen on angiogenesis.METHODS Different dosages of Chuangling liquid and its collagen loaded liquid were detected for through the chicken embryo allantois membrane model(CAM) by the optimal effects of angiogenesis. The vascular endothelial cell proliferation experiment (MTT)， the migration assay and the protein expression angiogenin1 (Ang1)， vascular endothelial growth factor (VEGF) and integrin(Integraav) with Western blot method were used for the detection of mechanism.RESULTS The CAM experiments showed that the angiogensis in 0.2 mL and 0.3 mL Chuangling liquid groups were significantly less than that of control group(P<0.05)， while there was no significant difference between 0.2 mL and 0.3 mL Chuangling liquid group. This antiangiogenic effects were reversed by the same dosage of Chuangling liquid loading into collagen with their effects reaching to the effects of control group. The 0.2 mL dosage of Chuangling liquid loading into collagen and/or loading into the collagen were chosen for the further experiment. The HUVEC proliferation rate of control group increased 86%， as compared with the group of Chuangling liquidwhich decreased. After loading into collagen， the proliferation rate was increased significantly higher than the results of control group with about 24%(P<0.01). The cell migration movement of 12hours， 24hours in the group of Chuangling liquidwere significantly lower than those in other group. When loading into collagen， cell migration rate began to accelerate and the migration rate significantly faster than that of control group on 24hours(P<0.01); Mechanism detection showed that the protein expressions of Chuangling liquidwith Integraаv was significantly lower than the results of control group， and the higher of Ang1 expression. After loading into collagen， the protein expressions of Integraаv， VEGF and Ang1 were increased significantly.CONCLUSION The study showed that negative effects on vascular endothelial cell migration and proliferation and thus inhibiting angiogenesis. Chuangling liquid loaded into Collagen may reduce the inhibition to angiogensis and be beneficial for blood vessels formation， in order to improve wound healing.
Different Effects of Rhizoma Pinelliae Processed by Ginger or Decocting on Gestation and Embryonic Development of Mice
XU Jian-ya, XIE Hui-hui, SHAN Jin-jun, XU Shan, WANG Shou-chuan
2013, 29(3): 255-258.
[Abstract](975) [PDF 710KB](662)
Abstract:
OBJECTIVE To study the different effects of Rhizoma Pinelliae(RP) processed by ginger or decocting on the gestation and embryonic development of mice， thus investigating the influential factors on abating toxicity of RP. METHODS Four groups were designed in which mice were treated respectively with PR suspension， ginger processed PR (GPPR) suspension， PR decoction and drinking water. Acute toxicity testing and Teratogenic sensitive period reproductive toxicity testing were used to compare the acute toxicity and effects on gestation and embryonic development of mice. RESULTS In acute toxicity testing， the mortality in mice were respectively 50% and 22.2% in PR suspension group and PR decoction group. However， there was not any significant deviation between the control group and GPPR suspension group. In terms of reproductive toxicity testing， the maternal mortality of PR suspension group (6 g/kg) reached 30%， while gestational maternal weight gain was significantly lower and the tail length (TL) of embryos was significantly shorter than the control group. There were no any maternal effects but the crown-rump length (CRL) of embryos was significantly shorter than the control group after treated with PR decoction (30 g/kg). There were no significant differences between the GPPR suspension Group (6 g/kg) and the control group.CONCLUSION Acute toxicity， maternal toxicity and embryo toxicity were all identified within the PR suspension group while none maternal toxicity was detected within the PR decoction group， however， the growth and development of embryos were affected in the PR decoction group. There was not any maternal toxicity or embryo toxicity in the GPPR suspension group.
Effect of Ganduqing Granule on Allergy
XUShi-Jun, XIONGMin, HUYong, ZHANGYin-Jie, JUShao-hua, DAIYong, WANGFei, ZHANGTing-mo
2013, 29(3): 259-261.
[Abstract](986) [PDF 336KB](686)
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OBJECTIVE To analyze the anti-allergy effect of Ganduqing granule and to provide experimental basis for its clinic application.METHODS The influence of Ganduqing granule on rat's skull Periost mast cells degranulation was observed and Passive skin allergy model in rats was prepared to observe the effect of Ganduqing granule on allergy.RESULTS In the rat's skull Periost mast cells degranulation rate， compared with the model group， the high-，medium-and low-dose Ganduqing granule groups were significantly decreased(P<0.05).The data of rats skin optical density in antisera injection site wew analyzed by one-way ANOVA， which showed that there was significant difference among groups in 1∶2 antisera injection site (P<0.05)， but there was no significant difference among groups in 1∶1 antisera injection site. In the PCA 1∶2 antisera injection site， compared with the model group， the optical density in the high-，medium-and low-dose Ganduqing granule groups were significantly decreased(P<0.05).CONCLUSION Ganduqing granule has a significant effect on allergy.
Study on optimal screening of rat model with blood stasis due to cold syndrome
XIE Bo, DENG Hai-shan, YU Bin, FANG Tai-hui
2013, 29(3): 262-265.
[Abstract](1344) [PDF 2685KB](1041)
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OBJECTIVE To investigate the effects of freezing time on setting up a rat model of blood stasis due to cold and to provide an experimental model for the study of blood stasis due to cold syndrome with Chinese medicine.METHODS The rats were divided randomly into 5 groups， namely control group， 5-day group， 10-day group， 15-day group and 20-day group. Except those in control group， the rest rats in other groups were induced blood stasis due to cold syndrome by kept in 0 ℃ ice water lasting for 15 mins one time a day. The complete blood count indexes， ovary index， uterus index and blood rheology were carefully observed.RESULTS Compared with control group， the erythrocytes of rats in 5-day and 10-day group were dramatically reduced， the APTT dramatically extended (P<0.01). In 5-day group， plasma viscosity， blood viscosity and erythrocyte aggregation index (EAI) reduced dramatically， erythrocyte deformability improved significantly， hemoglobin decreased remarkably， and uterus index declined significantly (P<0.05~0.01).The 10-day group also showed that the percentage of neutrophils increased significantly and TT shortened remarkably (P<0.05~0.01). In 10-day and 15-day group， both of their HCT reduced obviously. HBX of 10-day group declined， while that of 15-day group increased significantly (compared with the control group， P<0.01). Besides， TT obviously shortened (P<0.05)， plasma viscosity increased significantly (P<0.01)， and APTT also tended to be short in 15-day group. When frozen time lasted for 20days， PT and APTT were prolonged significantly (P<0.01).But rectal temperature of rats in each group had no significant change during the whole modeling process (compared with control group， P>0.05).CONCLUSION Making model on the 10th and 15th day of modeling， coagulative function improved， and abnormal hemorheology was the most obvious in 15-day group compared with other groups. The modeling approach may provide new ideas for the research of blood stasis due to cold syndrome.
Study on Separation and Enriching Total Flavonoids and Total Alkaloids in Compound Gankang Tablet by Macroporous Adsorption Resin and Ion Exchange Resins
HUJing-wen, WANGQiong, DILiu-qing
2013, 29(3): 266-270.
[Abstract](1013) [PDF 627KB](834)
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OBJECTIVE To separate and enrich the total flavonoids and total alkaloids from compound Gankang tablet.METHODS Combined applying macroporous resin and sodium cation resin to separate the total flavonoids and the total alkaloids respectively from the prescription， then verify the process.RESULTS Combination macroporous resin and sodium cation resin contributed to separate and enrich total flavonoids and total alkaloids.CONCLUSION It was feasible to use macroporous resin and sodium cation resin to separate total flavonoids and total alkaloids from compound Gankang tablet.
Detecting Oleanolic and Ursolic Acid with UPLC in Chaenomelis Fructus from Xuancheng
SHEN Pan-pan, XIE Xiao-mei, ZHANG Sheng-long, ZHU Chen-chen
2013, 29(3): 271-273.
[Abstract](958) [PDF 520KB](924)
Abstract:
OBJECTIVE To transform and develop high performance liquid chromatograph(HPLC)， to establish ultra performance liquid chromatography (UPLC) for determinating the contents of oleanolicacid (OA) and ursolicacid (UA) in Chaenomelis Fructus and to compare UPLC with HPLC.METHODS In the UPLC， ACQUITY UPLC TM BEHC18 column(100 mm×2.1 mm， 1.7 μm) was adopted， and the column temperature was 25℃.The mobile phase consisted of methanol-water-glacial acetic acid-triethylamine (254∶46∶0.1∶0.05) with a flow rate of 0.3mL/min.The detection wave length was 210nm， and injection volume was 1 μL in UPLC system. A Shim-pack CLC-ODS(M) column (250mm×4.6mm，5μm) was utilized in HPLC system， and a mixture of methanol-water-glacial acetic acid-triethylamine (265∶35∶0.1∶0.05) was regarded as mobile phase with a flow rate of 0.6mL/min.The Injection volume of HPLC was 20μL.RESULTS The content of oleanolicacid (OA) and ursolicacid (UA) in Chaenomelis Fructus of different picking time detected by HPLC was almost as same as that by UPLC. Compared with HPLC， UPLC provided shorter analysis time， cost less solvent consumption with better sensitivity.CONCLUSION UPLC may replace HPLC for the determination of OA and UA in Chaenomelis Fructus.
Approach on Professor Xiao Shaoqing's Research Theory of the Treatment of Difficult Diseases
LI Hong-da
2013, 29(3): 274-276.
[Abstract](900) [PDF 518KB](686)
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Xiao Shaoqing， Professor of Nanjing University of Chinese medicine， has practiced medicine for more than 60 years and formed a broad thought on diagnosing and treating difficult diseases with significant effects. Firstly， guided by Qihuang， Pro. Xiao took regulating spirit as the most important. Secondly， he adhered to treatment through relieving the primary and the secondary aspects by adopting both acupuncture and Chinese medicine. Thirdly， postnatal base was carried through to the end during treating diseases. Fourthly， Pro. Xiao kept broadening the thought by absorbing the essence in western medicine. Lastly， he always took the three-section acupuncture methods and penetration needling technique when practicing needling.
Research on Clinical Experience of Professor Tang Shu-hua in Syndrome Differentiation and Treatment of Heart Failure Based on Integrated Chinese and Western Medicine
ZHU Xian-hui
2013, 29(3): 277-279.
[Abstract](900) [PDF 392KB](848)
Abstract:
This paper summed up the academic thought and clinical experience of professor Tang Shu-hua in syndrome differentiation and treatment of heart failure based on integrated Chinese and western medicine. An overview was made which included the basic understanding of professor Tang Shu-hua on heart failure etiology and pathogenesis， the unique thought of diagnosis and treatment of combining diseases with differentiation of syndromes of heart failure as well as the experience of application of formula， such as Huangqi Shengmai beverage， Wuling powder and Qiangxin mixture. Meanwhile， this paper stressed that more attention should be paid to clinically interpenetration of properties and principles， such as supplementing Qi， being careful in warming yang， being appropriate in nourishing yin， avoiding circulation and keeping flowing in promoting diuresis， and to the characteristics of heart failure pathophysiology and risk factors for cardiovascular disease.
Effects of Xingnaojing Injection on hs-CRP and IL-6 in the Serum of Patients with Acute Cerebral Hemorrhage
ZHUChun-ran, XUChong-bai
2013, 29(3): 280-282.
[Abstract](930) [PDF 389KB](660)
Abstract:
OBJECTIVE To explore the relation between hs-CRP， IL-6 in the Serum and acute cerebral hemorrhage， observe the influence of Xingnaojing Injection on hs-CRP and IL-6， expound the possible mechanism of Xingnaojing Injection for treating cerebral hemorrhage. METHODS 90 cases with acute cerebral hemorrhage were randomized into experimental group and control group. The control group were given regular treatment with ICP-lowering and styptic drugs. Experimental group were given Xingnaojing Injection on the basis of regular treatment. After 14 days' treatment， venous blood of 45 patients in experimental group and 45 patients in control group was drawn. Immunoturbidimetry was applied for detecting hs-CRP and electrochemiluminescence was applied for detecting IL-6. RESULTS The change of hs-CRP and IL-6 presented decline curve. The decline in the treatment goup is much more obvious (P<0.05~0.01). There was significant difference when the value of hs-CRP and IL-6 on the 3ir day (P<0.05). CONCLUSION Xingnaojing Injection has notable therapeutic effects on acute cerebral hemorrhage and it is probably an effective inhibitor and nervous protective agent of CRP and IL-6.
Clinical Observation of 62 Cases of Shoulder-hand Syndrome after Cerebral Infarction Treated by Herbal Soaking plus Acupuncture and Massage Therapy
LILe-jun, CHENLi-ping, LIUXiao-li, XIAOHui, HUANGRong, CHANGHui, LIYu-mei, YANGChong-he
2013, 29(3): 283-286.
[Abstract](1113) [PDF 399KB](670)
Abstract:
OBJECTIVE To observe the clinical effects of herbal soaking plus acupuncture and massage therapy in the treatment of shoulder-hand syndrome after cerebral infarction. METHODS 122 cases of shoulder-hand syndrome after cerebral infarction were randomly divided into the treatment group(62 cases) treated with herbal soaking plus acupuncture and massage therapy， and the control group(60 cases) treated with single rehabilitation therapy. After the treatment， observing the clinical effect by Fugl-Meyer， NPS， Ashworth， SAS， SDS as well as SS-QOL scores， thus evaluating the life qualities of patients. RESULTS Compared with pre-treatment， clinical effects of the two groups were remarkable to different degrees. Motor function of upper limb had improved (P<0.05， P<0.01). Symptoms (including pain and spasm of upper limb) were relieved and cognitive function as well as SS-QOD scores were improved (P<0.05， P<0.01). In terms of curative effect， the treatment group was superior to the control group. CONCLUSION Herbal soaking plus acupuncture and massage therapy can improve the curative effect of shoulder-hand syndrome after cerebral infarction.
Intervention Study on Inflammatory Factors of Rabbits with Atherosclerosis by Reinforcing the Kidney， Promoting Blood and Resolving Phlegm
YANG Zhi-gang, ZHANG Wei-bin, CHENG Hong
2013, 29(3): 287-289.
[Abstract](958) [PDF 367KB](704)
Abstract:
OBJECTIVE The study investigates the mechanism of the inflammatory factors of the atherosclerosis' (AS) plaques of experimental rabbit models by therapy of tonifying the kidney and activating blood and dissipating phlegm， and to discuss the advantages of combination of TCM with western medicine in the treatment of AS.METHODS The study selected 40 healthy male purebred rabbits of New Zealand， 2 weeks after feeding observation adaptability， all rabbits were randomly divided into 4 groups， Model Group(MG)，Traditional Chinese Medicine Group (TCMG)，West Medicine Group(WMG)， Zhong Xi Group(ZXG).After the treatment， three treatment groups' rabbits were observed on the levels of the blood lipid and lipoprotein， and five serum inflammatory factors（including Fg，hs-CRP，IL-18，MCP-1，MMP-9）.RESULTS Compared between each treatment groups， with MG， the level of the five inflammatory factors of the three treatment groups declined significantly(P<0.01).The group of ZXG was superior to the groups of TCMG and WMG in decreasing the level of five inflammatory factors(P<0.01).And compared with TCMG， the group of WMG was superior to the TCMG in decreasing the level of the hs-CRP(P<0.05)，but the other four indicators had no differences(P＞0.05).CONCLUSION The therapy of tonifying the kidney and activating blood and dissipating phlegm combined with the Atorvastatin calcium may be superior to the groups of TCMG and WMG in decreasing the levels of five inflammatory factors. We can infer that the levels of inflammatory factors may be a important element to decide plaque vulnerability.
HPLC Simultaneous Determination of Puerarin， Paeoniflorin and Glycyrrhizic Acid in Fufang Zhixie Granule
ZHU Xing-guang, MENG Yong-qin, ZHOU Qian
2013, 29(3): 290-292.
[Abstract](571) [PDF 768KB](1)
Abstract:
OBJECTIVE To develop a method for the simultaneous determination of Puerarin， paeoniflorin and glycyrrhizic acid in Xiao'er Zhixie granule.METHODS The analysis was carried out on a C18（4.6 mm×250 mm，5 μm）column; the mobile phase was composed of methanol and 0.1% phosphoric acid aqueous for gradient elution（26︰74~81︰19）. The flow rate was（0.8~1.0） mL/min and the column temperature was 25 ℃.The detection wavelength was set at 239 nm.RESULTS The results showed that 3 effective components all separated well and showed good linearity， the average recoveries were between 95%~105%.CONCLUSION The method is simple and can be used to determine the above 3 components with satisfactory accuracy and repeatability. It can be used for the quality control of Fufang Zhixie granule.
Research Progress for the Treatment of Ulcerative Colitis
CHENXi, TIANYao-zhou, XIAJun-quan
2013, 29(3): 293-296.
[Abstract](597) [PDF 758KB](2)
Abstract:
The Application of Carrier on Improving Oral Nanoparticles Intestinal Absorption
JIANG Wei-ming, GUO Sheng-rong
2013, 29(3): 297-300.
[Abstract](591) [PDF 761KB](1)
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