南京中医药大学学报

On Syndrome Diagnosis of Hemorrhagic Stroke Based on Pathogenesis

GUOWei-feng, LIGuo-chun, LITing-ting, SUNRong-rong, WUMian-hua

2013, 29(1): 1-4.

[Abstract](664) [PDF 374KB](594)

Abstract:
Differentiating the syndrome of a disease according to the symptoms is the basic pattern of traditional Chinese medical (TCM) syndrome-differentiating system， with the advantage and feature of flexibility ， and the disadvantage of being difficult to be regulated and unified. Based on determining the pathogenesis according to syndrome differentiation， this paper has presented the conception of pathogenic syndrome element which is the pathogenic element of a syndrome in a certain disease range， with the function of generalizing/reflecting the pathogenic characteristics of the syndrome. Springing from the pathogenic elements， the pathogenic syndrome elements have a limited number and clear content， so it's completely feasible to set up their regular syndrome-differentiating diagnostic standard， of which the premise is making a diagnostic scale of pathogenic syndrome element. Taking hemorrhagic stroke for example， this paper has studied the method and procedure of making the pathogenic syndrome element diagnostic scale， and has further studied the syndrome-differentiating diagnostic method based on the combination of pathogenic syndrome elements. The results have reflected the union of syndrome-differentiating flexibility and syndrome standardization， so as to adapt to the complicated and changeable situation of TCM clinical syndromes， and open up a new way of establishing the TCM differential model with the union of syndrome standardization and syndrome-differentiating flexibility.

Application of Oxidative Stress Mechanism in phlegm-wet Constitution

LI Ling-ru, WANG Ji, LI Ying-shuai, ZHANG Hui-min, ZHENG Lu-yu, YANG Ling-ling, ZHANG Yan, WANG Qi

2013, 29(1): 5-8.

[Abstract](689) [PDF 417KB](627)

Abstract:
Phlegm-wet block is associated with the formation of phlegm-wet constitution，and also it is a kind of pathogenic factors， which can block qi-blood flowing，affect the function of organs，and finally result in Metabolic and cerebrovascular diseases. Increased oxidative stress is a key pathophysiological feature of those diseases， and the early antioxidant therapy can prevent them. This paper discusses the relationship between phlegm-wet constitution and oxidative stress from aspects of influencing factors and pathogenic mechanism， and holds that there are many similar influence factors between phlegm-wet constitution and oxidative stress ，such as life style， sports and age，etc. The decrease of antioxidant ability may be the formation basis of phlegm-wet constitution，the imbalance of redox may play an important role in the pathogenesis of phlegm-wet constitution，and oxidative damage may be the intrinsic mechanism which relate to the pathogenic diversity of phlegm-wet constitution. Exploring phlegm-wet constitution through oxidative stress can provide the important basis for disease prevention.

Exploration of Resolving Necrotic Tissues and Promoting Growth of New Tissues

MA Yong-qi, JU Xiao-rong

2013, 29(1): 9-11.

[Abstract](630) [PDF 378KB](637)

Abstract:
Through reviewing the academic origin of the therapy of resolving necrotic tissues and promoting new tissues' growth as well as summarizing the etiology and pathogenesis of chronic ulcer disease， it is believed that the irregular flow of qi and blood， disharmony of nutritive qi and defensive qi， malnutrition of muscle and skin are all due to the interaction of toxin， necrotic tissues， blood stasis and xu. In line with the main pathogenesis， the chief methods of remove toxin， eliminate necrotic tissues， resolve stasis and tonify deficiency are pertaining to the resolving the necrotic tissues and promoting the new tissues' growth. Resolving necrotic tissues is combined with promoting growth of new tissues. Promoting growth of new tissues is contained in and also assisted by resolving necrotic tissues， two of which are interdependent and have corresponding emphases. Exploring the therapy of resolving necrotic tissues and promoting new tissues' growth and making it conforming to the clinical ulcer healing rules can be helpful for its cure. At the meanwhile， the advantages and distinguishing features of traditional Chinese medicine in ulcer tissue's regeneration can be identified， and better guidance for traumatic scientific research and treatment of ulcer will be made.

Influence of Clearing Heat， Cooling Blood and Relieving Toxin Method on Blood Coagulation of Patients Bitten by Long-Noded Pit Viper

LIU Zhi-kun, LI Qing-ping, ZHOU Wen-zhong, XIONG Zhong-rong

2013, 29(1): 12-15.

[Abstract](676) [PDF 536KB](719)

Abstract:
OBJECTIVE To observe influence of clearing heat， cooling blood and relieving toxin method on blood coagulation of patients bitten by long-noded pit viper. METHODS There were totally 69 cases of patients bitten by long-noded pit viper in Guilin Hospital of Traditional Chinese Medicine from May 2008 to May 2012， who were randomly divided into control group (36 cases) and treatment group (33 cases). The control group used the conventional treatment， while the treatment group added Xijiao Dihuang Decoction and Wuwei Xiaodu Decoction. Platelet (PLT)， thrombin time (TT) and prothrombin time (PT)， activated part clotting live enzyme time (APTT) and fibrinogen (FIB) of patients in two groups were observed 12， 24， 36 and 48 hours after administration. RESULTS PLT， TT， PT， APTT and FIB were not significantly different 12 hours after administration. But after 24 hours， PLT， TT and APTT in treatment group were statistically significant compared with control group (P<0.05). TT and APTT of two groups at 36th hour were statistically significant (P<0.05). Blood coagulation functions in two groups at 48th hour were basically returned to normal level. The rising trend of PLT in treatment group from 12h to 24h was more obvious compared with control group， while the change tendency of FIB in two groups was similar. TT and APTT in treatment group from 24th to 36th hour declined more apparently than control group. The change trend of PT in two groups did not show significant difference. CONCLUSION Clearing heat， cooling blood and relieving toxin method promotes the blood coagulation function recovery of patients bitten by long-noded pit viper.

Fuhe Beihua Fang for Postoperative Syndrome of 40 Patients with Primary Liver Cancer after Intervention

MOChun-mei, RONGZhen, HUZhen-bin, LVJian-lin, CHENXin-ri, WEIHai-xia

2013, 29(1): 16-18.

[Abstract](762) [PDF 356KB](707)

Abstract:
OBJECTIVE To observe the clinical effects of Fuhe Beihua Fang on postoperative syndrome of 40 patients with primary liver cancer after interventional therapy. METHODS 80 cases were randomly divided two groups: control group and treatment group with 40 cases in each. Treatment group was given Fuhe Beihua Fang after undergoing hepatic artery chemotherapeutic embolism (TACE); while control group didn't. Then we observed integration changes of symptoms before and after treatment， and indexes of liver function (including alkaline phosphatase， AST and ALT)， Karnofsky score and change of tumor marker AFP. RESULTS Treatment group showed better effects on fever， nausea and vomiting， abdominal distension， jaundice and hypochondriac pain， compared with control group (P<0.05)， significantly improved patients' life quality (P<0.05)， relieved impairment of liver function(P<0.05) and lower ed AFP(P<0.05). CONCLUSION Fuhe Beihua Fang can effectively improve clinical symptoms of patients， lower tumor marker AFP caused by carcinoma， improve indexes of liver function and life quality of patients.

Randomized Controlled Trial of Combination of Internal and External Therapy for the Attack Stage of Bronchial Asthma

SHI Shao-shun, YU Xue-feng, YU Guo-qiang, YAO Ming, DING Xiao-huan, LI Ding-lei, GUO Zhen-wu

2013, 29(1): 19-21.

[Abstract](704) [PDF 342KB](693)

Abstract:
OBJECTIVE To evaluate the curative effect and safety of the combination therapy of Qingfei Pingchuan Granule and Guben Qingfei Plaster in treating the attack stage of bronchial asthma (the heat syndrome). METHODS 120 cases of bronchial asthma in the attack stage (the heat syndrome) were randomly divided into experimental group and control group which received the combination therapy of Qingfei Pingchuan Granule and Guben Qingfei Plaster and the combination therapy of Theophylline Sustained-release Tablet and Budesonide Powder Inhalation respectively. Then the changes of indexes， such as TCM symptoms integral， disease severity classification， asthma control test (ACT) and eosinophils (EOS) count in peripheral blood， before and after treatment were observed. RESULTS Curative effects on the disease: the group with the combination therapy of Qingfei Pingchuan Granule and Guben Qingfei Plaster (experimental group) showed a markedly effective rate of 78.18% and a total effective rate of 92.73%， while the group with the combination therapy of Theophylline Sustained-release Tablet and Budesonide Powder Inhalation (control group) had a 80.39% markedly effective rate and a 90.20% total effective rate. The difference between two groups had no statistical significance (P>0.05)， meaning the two therapies had equivalent curative effects. Curative effects on the TCM syndrome: the markedly effective rate of experimental group was 63.63%， and the total effective rate was 92.73%， while those of control group were 52.94% and 88.24% respectively. The difference between two groups had no statistical significance (P>0.05)， which meant that the two therapies had equivalent curative effects on the TCM syndrome. There was no difference between two groups in the aspects of disease severity classification， asthma control situation and the influence on EOS count (P>0.05). CONCLUSION The curative effect of Qingfei Pingchuan Granule combined with Guben Qingfei Plaster is equal to that of Theophylline Sustained-release Tablet combined with Budesonide Powder Inhalation， and no obvious adverse reaction is found， which makes a new option for clinical medication possible.

Classification and Quantitative Diagnosis Factor Analysis on Chronic Prostatitis Symptom

CHEN Jian-she, SUN Zi-xue, HAN Jun-chang

2013, 29(1): 22-24.

[Abstract](637) [PDF 364KB](779)

Abstract:
OBJECTIVE To research the different types of symptoms and the rule of quantizes diagnosis. METHODS Factor analysis method was applied to study the four diagnosis methods of 408 cases with chronic prostatitis， and the factors were abstracted to measure the symptoms loaded for the clarification of the relationship between factors and symptoms. RESULTS Altogether 11 factors were chose from the four diagnosis messages， and 4 of them were found to correspond to damp-heat downing flow， stagnation of qi and stasis of blood， deficiency of liver and kidney yin， and deficiency of kidney yang symptoms， respectively. The four symptoms were weighted and the main symptoms and sub-symptoms were concluded. CONCLUSION Factor analyses provide objective evidence for the type and quantities of chronic prostatitis clinical diagnosis.

Alternation of Macrophage Cytokines in the Progress of Mice Depression and the Regulation of Acupuncture

HONGMin, HUYan, ZHENGJie, DINGZhao-yun, NIUYan, SHAOJian-hua, WANGLing-ling

2013, 29(1): 25-28.

[Abstract](614) [PDF 893KB](624)

Abstract:
OBJECTIVE To study the changes of macrophage-related cytokines in the development of depression， and to explore the immunological mechanism of acupuncture on treating depression.METHODS Chronic unpredictable mild stress (CUMS) was employed to induce depression in mice and open-field test and sucrose preference test were operated during the progress of depression. Simultaneously， the peritoneal macrophages were isolated from mice and were cultured under the stimulation with LPS， ELISA method were used to measure the contents of TNF-α， IL-1β and IL-6 in the supernatant of peritoneal macrophages. Depression mice were given treatment of acupuncture， and the effect of behavior change was observed and the content of macrophage cytokine production was measured respectively.RESULTS 21 days after making model， the model of chronic unpredictable mild depression was generally developing， the secretion ability of IL-1β and IL-6 in the middle or late stage increased significantly， while the ability of TNF-α did increase but not that obvious. After treatment of acupuncture， behavior indicator was improved and meanwhile the ability of secreting TNF-α， IL-1β， IL-6 decreased significantly. CONCLUSION The ability of modeling mice secreting inflammatory cytokines by peritoneal macrophages increased in the formation of CUMS depression， which acupuncture can significantly regulate might be one of the mechanisms in improving behavior change.

Inhibiting Effect of Lycium Barbarum Extracts on Sub-RPE Deposit Formation in High-fat Diet and Oral Hydroquinone Model Mice

HUANGBing-lin, HANGLi, ZHENGShi-zhong, LIWei, ZHUChang-le, WEIYuan-hua, XUXin-rong

2013, 29(1): 29-34.

[Abstract](680) [PDF 1207KB](856)

Abstract:
OBJECTIVE To investigate the inhabiting effect of lycium barbarum extracts on sub-RPE deposit formation in high-fat diet and oral hydroquinone (HQ) model mice. METHODS 40 eight-month-old C57BL/6 female mice， 8 were fed normal diet as aging control group; 32 were fed a high-fat diet for 6 months followed by HQ (0.8%) in the drinking water for 3 months. Model mice were randomly divided into model control group (8 mice) and 3 treatment groups (8 mice each group). Lycium barbarum extracts were given through different dose lavage one time every day(high:3.75g/kg/d， middle:2.50g/kg/d， low:1.25g/kg/d) for 3 months. At the end of the experimental period， the mice were killed and the eyes were immediately removed. Transmission electron microscopy was used to observe sub-RPE deposit formation and Bruch membrane (BrM) thickness， semiquantitative grading of deposit severity was performed; The mRNA and protein expression of cathepsin B（Cat B）and cystatin C（Cys C）were detected by real-time PCR and western blot respectively. RESULTS Mice fed a high-fat diet and HQ showed RPE damage was severe， formation of sub-RPE deposits was increasing， in addition， BrM was typically thickened，the severity in model control group was statistically greater than in the aging control group. Lycium barbarum extracts could reduce RPE damage， inhibit sub-RPE deposit formation and thickening of BrM. Compared with the aging control group， the mRNA and protein expression of Cat B and Cys C were significantly higher in the model control group. Expression of Cat B and Cys C were down regulated by the lycium barbarum extracts， and the effect was enhanced as the dosage was increased in a dose dependent manner， there was an especially significant reduction of Cys C expression.CONCLUSION Lycium barbarum extracts could reduce RPE damage， and inhibit sub-RPE deposit formation and thickening of BrM in high-fat diet and oral HQ mice model. The mechanism may be to reduce generation of ROS， down regulate the expression of Cat B and Cys C， and comprehensive actions of Cat B and Cys C shift to the function of Cat B.

Effect of Total Flavonoids in Persimmon Leaf on the Expression of Arteriosclerosis ApoE^-/- mice's Adhesion Molecule

BAIWei-na, CHENYing-zhu, YANJing-hui, YANYu-ping, SHIWei, XIELi

2013, 29(1): 35-38.

[Abstract](674) [PDF 604KB](626)

Abstract:
OBJECTIVE To explore the effect of total flavnoids of persimmon leaves on the expression of adhesion molecule in ApoE^-/- mice with thoracic and abdominal atherosclerosis.METHODS ApoE^-/- mice were divided into model group and total treatment group randomly， putting c57BL/6 mice as control group， making the proximal part of the thoracic and abdominal aorta into continuous frozen section， which would be stained and observed about the tectology changes. The serum changes would also be tested. Immunohistochemistry stain method was used to observe the distribution of the adhesion molecule expression in the certain pathological location. RESULTS HE stained showed that the control group's enangium is smooth without AS changes， and the model group had large area plaques， extending into the lumen and caused stenosis. The treatment group alleviated without obvious lumen stenosis. The serum tests show there was no apparent lipdemia differences between the model group and treatment group. The immunohistochemisty tests show the control group mice aorta had no positive stained granule expression， and the model group had the positive expression in the shoulder and the fiber head of the plaque. The treatment group had a rare positive expression which mainly intra the vessel. The model group were lower than the treatment group apparently (P<0.05).CONCLUSION Total flavnoids of persimmon leaves can suppress the expression of adhesive molecule (P-Selection， ICAM-1， VCAM-1， et al). Furthermore to alleviate the pathogenic changes of AS， but it cannot lower the lipdemia.

The Study of Euryale Seed Extracts Protecting SH-SY5Y Neurocytes Damage and Their Invitro Antioxidant Activities

SHEN Bei, YUAN Dong-ping, WU Cheng-ying, HE Xiao-xiao, WANG Xin-sheng, WU Qi-nan

2013, 29(1): 39-43.

[Abstract](687) [PDF 855KB](646)

Abstract:
OBJECTIVE To investigate the protective effects of euryale seed 80% ethanol， ethyl acetate and n-butyl alcohol extracts on the H₂O₂-induced damaged SH-SY5Y neurocytes and their invitro antioxidant activities.METHODS The H₂O₂-induced damaged SH-SY5Y neurocyte models were cultured outside of the body. Cell Counting Kit-8 (CCK-8) was used to determine the effects of the above euryale seed extracts with different concentrations on the activities of the H₂O₂-induced damaged SH-SY5Y neurocytes， and then their inhibition rates were calculated. The in-vitro antioxidant experimental method was applied to detecting the capabilities of the three extracts in removing DPPH， ABTS radicals and deoxidating three price iron ions.RESULTS Within the crude drug concentration range of 10~30mg/mL， euryale seed extracts in 80% ethanol， ethyl acetate and n-butyl alcohol showed obvious functions of inhibiting the oxidant damage action of H₂O₂ on the SH-SY5Y neurocytes (P<0.05， P<0.01). In addition， the above euryale seed extracts could markedly clear away DPPH， ABTS radicals and deoxidate three price iron ions， and among which， the n-butyl alcohol extract had the most powerful action.CONCLUSION Having an excellent antioxidant activity， euryale seed can protect the SH-SY5Y neurocytes from the damage of H₂O₂. Its active extracting solvents include ethanol， ethyl acetate and n-butyl alcohol. After a comprehensive comparison， it shows that the n-butyl alcohol extract has the most powerful function.

The Effect of Hederagenin on the Proliferation， Adhesion， Invasion and Migration of Human Colon Cancer Cells LoVo

LIUBAOXin-zi, WANGRui-ping, ZOUXi, ZHOUJin-yong

2013, 29(1): 44-47.

[Abstract](649) [PDF 605KB](604)

Abstract:
OBJECTIVE To explore the effect of hederagenin on the proliferation， adhesion， invasion and migration of human colon cancer cell line LoVo in vitro.METHODS MTT assay， adhesion experiment， Transwell room invasion test， and erasion trace test were used to observe the effect of hederagenin on the proliferation， adhesion， invasion and migration.RESULTS ①Hederagnin inhibited the proliferation of LoVo cells. Compared with control group， the inhibition ratio had been significantly increasing with the increase of the drug concentration， which showed a concentration dependence (P<0.01). ②Hederagnin remarkably reduced the adhesion capability of LoVo cells (P<0.01)， and along with the increasing drug concentration and time， the inhibition ratio increased. ③Compared with control group， the number of invaded cells was significantly decreased (P<0. 01). The higher the drug concentration was， the less cells were invaded. ④The cell healing degree had an obvious group difference， and the higher the drug concentration was， the worse the healing degree was， which indicated that migration ability was greatly affected.CONCLUSION Hederagenin can remarkably inhibit the proliferation， adhesion， invasion and migration of LoVo cells.

Effect of Astragaloside IV on IL-1β Expression of Degenerative Joint Cartilage in Human Knee Osteoarthritis

TANGXiao-chen, YUFeng, SUNShu-long, JIANGHong, GONGZheng-feng, XUJian-an, WANGYong-jun

2013, 29(1): 48-52.

[Abstract](759) [PDF 970KB](653)

Abstract:
OBJECTIVE To study the effect of Astragaloside IV on the IL-1β expression of degenerative joint cartilage in human knee osteoarthritis(OA). METHODS Degenerative joint cartilage cells of postoperative knee OA patients were isolated， cultured and passaged in vitro. The third generation cells were identified by HE and type Ⅱ collagen immunofluorescence staining， and then were stained by IL-1β immunofluorescence. They were divided into 6 groups after the cell degenerative situation was observed (Group A: cartilage cells + DMEM control group; Group B: cartilage cells + 25 mmol/L Astragaloside IV; Group C: cartilage cells + 50 mmol/L Astragaloside IV; Group D: cartilage cells + 75 mmol/L Astragaloside IV; Group E: cartilage cells + 100 mmol/L Astragaloside IV; Group F: cartilage cells + 500mmol/L Astragaloside IV)， and real-time fluorescent quantitative PCR was used to detect IL-1β expressing mRNA in degenerative cartilage cells of every group in 4 h， 8 h， 24 h， 48 h and 72 h. RESULTS ①Morphologic observation. Spindle cells and polygonal cells were found in the initial generation cells at the same time. Nucleus located at the side of the cell， closing to the membranes， and the cell filaments were long. HE stained the cytoplasm into red and the membranes into blue. Toluidine blue stained the cytoplasm and membranes into dark blue. The positive type Ⅱ collagen stain was green fluorescence. The nucleus were restained by 4'，6-diamidino-2-phenylindole. Activated by fluorescence in various wave bands， the plasm and membranes presented clear green fluorescence， while the nucleus areas showed no obvious green fluorescence， which proved that type Ⅱ collagen mainly distribute in plasm and membranes， and the cultured cells were cartilage cells indeed. The clear green fluorescence could be seen after IL-1 immunofluorescence staining， and polygonal cells were dominated among cartilage cells. ②IL-1 mRNA expression. The IL-1 mRNA expression levels in different time showed difference (F=13.236，P<0.01); and those in different groups were different as well， Group A higher than Group B， C， D， E (P<0.05~0.01) and Group F higher than Group A， B， C， D， E (P<0.05~0.01); the factors of time and group had interaction effect (F=7.262，P<0.01). CONCLUSION Astragaloside IV with a certain concentration can delay the degeneration of cartilage cells by inhibiting IL-1 synthesis in the degenerative cartilage cells， but it with a excessive high concentration will speed up the degeneration by promoting IL-1 synthesis.

Research on Antiviral Effects of the Active Components of Radix Isatidis

SUNDong-dong, YANShi-hai, CHENJian-wei, LIXiang, HELi-wei

2013, 29(1): 53-55.

[Abstract](672) [PDF 364KB](890)

Abstract:
OBJECTIVE To study the antiviral activities of 30% EtOH-sinked polysaccharide I， 50% EtOH-sinked polysaccharide II and 70% EtOH-sinked polysaccharide III which are extracts of different parts of Radix Isatidis， D101 macroporous resin water-eluted part IV， 10% EtOH-eluted part V， 30% EtOH-eluted part VI， 50% EtOH-eluted part VII which from the water extract of Radix Isatidis， and compound slariciresinol-4， 4'-bis-O-β-D-glucopyranoside (CB) VIII and isolariciresino IX on influenza A virus A/PR8/34(H1N1).METHODS The anti-influenza A virus A/PR8/34(H1N1) in vivo experiment was applied. And the antiviral activities of extract parts and monomeric compounds were tested by measuring lung index and lung index inhibition rate. RESULTS II， III， IV， V， VI， VII， VIII and IX could obviously prolong the living time of mice infected by influenza A virus， and the results showed marked differences compared with control group (P<0.05). II， III， IV， V， VI， VII， VIII and IX could decrease the death rate of infected mice; at the meantime， II， VI， VII and VIII could also obviously reduce lung index (P<0.05)， and the lung index inhibition rates were 31.4%， 28.5%， 30.5%， and 34.0% respectively.CONCLUSION The extract II-IX of Radix Isatidis can protect the mice infected by influenza A virus， and CB may be the effective and index component with antiviral activity in Radix Isatidis.

Preliminary Evaluation and Preparation of Brucine Nanoparticles-in-microsphere System

WANGJian, CHENZhi-peng, CAIBao-chang

2013, 29(1): 56-59.

[Abstract](679) [PDF 639KB](705)

Abstract:
OBJECTIVE To find a long-acting preparation which can reduce the burst effect in intra-articular injection by preparing the brucine nanoparticles-in-microsphere system (Brucine-NiMS).METHODS Brucine nanoparticles (Brucine-Nps) were prepared by desolvation of BSA. Chitosan was used as an carrier and the nanoparticles were contained in the microsphere system by the spray drying method. The appearances of Brucine-NiMS were observed under the electron microscope and then the in vitro release characteristics were determined.RESULTS The formulation of Brucine-NiMS was as follows: BSA/CS (600KDA) 1∶5 (w/w) and the concentration of CS in the acetic acid was 1%. The spry drying conditions were as follows: The speed of the peristaltic pump was adjusted to 20%， the temperature of inlet was 120 ℃， and Q-Flow was 40 mL/h. The envelop rate， drug loading and particle sizes of Brucine-NiMS were 97.35%， 3.17% and 1.48 μm respectively. The appearance of Brucine-NiMS was rounding and the nature was stable.CONCLUSION The preparation method of Brucine-NiMS is stable and controllable， providing a new way to develop new dosage forms of drugs.

Preparation of Epimedins A， B， C from Epimedium brevicornum Maxim Using Middle Pressure Preparative System Combined Autopurification System

CUILi, SUNE, WANGJing-jing, JIAXiao-bin, WUFu-long

2013, 29(1): 60-62.

[Abstract](687) [PDF 538KB](639)

Abstract:
OBJECTIVE Middle pressure preparative system combined autopurification system was developed to purity Epimedins A，B，C as reference standards from the crude extract of Epimedium brevicornum Maxim.METHODS After the crude extract containing 5% flavonols was enriched using macropore resin， the obtained target fractions were subjected to middle pressure preparative system and then to autopurification system.RESULTS By repetitive injection of the enriched target fraction on to the preparative column， 2.4 g epimedin A as well as 14.8 g epimedin B and 1.7 g epimedin C were obtained from 4.5 kg Epimedium brevicornum Maxim raw herbs. The purities of all products were greater than 98%.CONCLUSION The middle pressure preparative system combined autopurification system technique for three target compounds are quite useful for the production of the reference standard series with good putity like epimedins A， B， C standards due to its rapid separation， high performance and more amounts of products obtained.

Research on the Content of Pharmacodynamic Components of Moutan Cortex by HPCE in Different Ages and Picking Periods

HU Yu-wen, CHAO Jian-guo, GU Wei, LIU Xun-hong, KONG Zhao-yan

2013, 29(1): 63-65.

[Abstract](639) [PDF 478KB](637)

Abstract:
OBJECTIVE To determine the best harvesting time for Moutan Cortex and provide basis of GAP production.METHODS HPCE was employed to determine the content of 2 pharmacodynamic components( paeonol and gallic acid) of Moutan Cortex at different ages and picking periods.RESULTS The contents of paeonol and gallic acid in Moutan Cortex were different in various harvesting time， and relatively higher in 5 or 6-year-old Moutan Cortex; all components contents were higher and more stable in September or October at the same harvesting year.CONCLUSION Concluding various factors， September or October is the best harvesting time for 5-year-old Moutan Cortex.

Analysis of and Dynamic Study on Volatile Ingredient of Folium Perillae by HSGC /MS

YANG Jun-hui, FAN Rong, LIU Xun-hong, FU Xing-sheng, XU Hu

2013, 29(1): 66-70.

[Abstract](607) [PDF 581KB](664)

Abstract:
OBJECTIVE To explore the dynamic accumulation of the volatile ingredient of folium perillae， and to provide scientific basis for its best time of the collection.METHODS HSGC/MS methods were used to analysis the volatile ingredients of folium perillae collected in different time and peak area normalization method was used to determine the relevant percentage composition of each ingredients. 10 active ingredients were used as target， and their dynamic changes were observed at the same time.RESULTS 52 ingredients were separated and identified initially， and among them， 20 ingredients were exists the whole stages. In different growing stages， the volatile ingredients accumulation of folium perillae follows certain rules.CONCLUSION The highest peak value of folium perillae volatile ingredients accumulation corresponds to the traditional picking time.

Study on Quality Standard of Wuwei Hugan Chewable Tablets

WANG Hui-juan, MAO Chun-qin, LU Tu-lin, WANG Ju, GU Juan-juan, WU Shuang-shuang

2013, 29(1): 71-74.

[Abstract](621) [PDF 548KB](640)

Abstract:
OBJECTIVE To establish quality standard of Wuwei Hugan Chewable Tablets.METHODS With chisantherin B， Deoxyschisandrin and Schisandra as control materials， Wuwei Hugan Chewable Tablet is identified by thin layer chromatography (TLC); the content of schisandrin， schisantherin B， schisantherin A， Deoxyschisandrin and Schisandrin B， are simultaneously determinated by HPLC.RESULTS Excellent chromatographic separation was achieved in the five tested lignans with good linearity (r>0.999 5) over the studied concentration ranges. The average extraction recovery (RSD)(n=9) was 99.90%(1.63%) for Schisandrin， 99.42%(1.72%) for Schisandrol B， 99.81%(2.07%) for Schisantherin A， 99.79%(1.91%) for Deoxyschisandrin and 100.57%(1.61%) for Schisandrin B.CONCLUSION Specific， sensitive and reliable TLC identification and HPLC content determination of Wuwei Hugan Chewable Tablets were established. The TLC and HPLC methods developed for simultaneous determination of five lignans are simple and valid. It can be used for comprehensively controlling quality of Wuwei Hugan Chewable Tablets.

Quality Standard Study on Jiangxiang Anwei Soft Capsules

WU Qu-fan, GAO Ming, LI Geng

2013, 29(1): 75-77.

[Abstract](763) [PDF 553KB](656)

Abstract:
OBJECTIVE To establish the controlling methods for quality standard of jiangxiang Anwei Soft Capsules(Rhizoma Alpiniae Officinari， Rhizoma Zingiberis， Radix Aucklandiae).METHODS Rhizoma Alpiniae Officinari， Rhizoma Zingiberis， Radix Aucklandiae were identified by thin-layer chromatography(TLC). The contents of costunolide and dehydro-costunolide were determined by High Performance Liquid Chromatography (HPLC) together. RESULTS Consimilitude showed a good linear relationship at the range of 0.103～1.030 μg( r=0.999 5，n=7），the average recovery was 97.27%， RSD was 1.10%;Dehydro-costunolide showed a good linear relationship at the range of 0.126～1.260 μg(r=0.999 6，n=7）， the average recovery was 97.90%，and RSD was 1.05%.CONCLUSION This method is accurate， reliable， and can be used for quality control of Jiangxiang Anwei Soft Capsule.

Medical Records of Professor ZHOU Zhong-ying Treating Lower Warmer Diabetes by Liuwei Dihuang Analogous Formulas

FANG Liang

2013, 29(1): 78-80.

[Abstract](723) [PDF 395KB](635)

Abstract:
This article selected 3 medical records of professor ZHOU Zhong-ying treating lower warmer diabetes by Liuwei Dihuang analogous formulas. Oedema is the major clinical manifestation of the previous 2 cases. Record 1 used Gui-fu-di-huang Wan and herbs that can warm kidney-yang， replenish qi， invigorate spleen and clear water; while record 2 used Liuwei Dihuang Pill with herbs that can replenish qi， activate the circulation of blood. Enuresis is the main symptom of the last case. As a result， the “three tonic herbs” in Liuwei Dihuang Pill is used， and also some tonic herbs that can nourish liver and kidney， and some astringent drugs that can control essence and urine emission.

Experimental Study on Professor WANG Shou-chuan Treating Asthma

DAIQi-gang, LIANGXiao-xin, AIJun, XUShan

2013, 29(1): 81-83.

[Abstract](637) [PDF 358KB](688)

Abstract:
Based on Qian Yi's view of lung dominant asthma， the national renowned experienced TCM doctor Wang Shou-chuan points that asthma is the representative of the lung system disease. The dominant mechanism of the disease is the disorder of qi. The sthenia type is caused by the pathogenic evil invading the body， and the asthenia type is caused by deficiency of qi and yin. For treating sthenia type， getting rid of the pathogenic evil and smoothing the free flow of qi can be used， and for the asthenia type， replenishing qi and nourishing yin for beneficing lung method can be chosen. Clinically， there are more sthenia type， and getting rid of evil and beneficing healthy qi methods are always used together. Appendix was taken as an example.

Professor Zhou Fu-yi's Experience of Treating Knee Osteoarthritis from Resolving Phlegm and Blood Stasis

YAN Pei-jun, WANG Pei-min

2013, 29(1): 84-86.

[Abstract](633) [PDF 371KB](637)

Abstract:
Knee Osteoarthritis (KOA) is a common disease in clinic affecting life quality， especially obese people after middle age. With years of great clinical experience， Professor Zhou Fu-yi holds that its main pathological mechanism is the deficiency of liver and kidney as well as interrelation between phlegm and stasis and suggests that reinforcing liver and kidney and dissipating phlegm and removing blood stasis should be applied in treating knee osteoarthritis with significant treatment effect and worth of clinical application.

Acupuncture for Syncope Caused by the Abstract of Nasal Packing Material

ZHOUXing-wei, ZHONGLun-kun, SUNYong-dong, HUWen-jian, XIEQiang

2013, 29(1): 87-88.

[Abstract](619) [PDF 317KB](624)

Abstract:
OBJECTIVE To observe the effect of acupuncture on syncope caused by abstract of the nasal packing material. METHODS 92 patients， who had operated resection of the lower deviation mucus of the nasal septum and needed another distending sponge abstract operation， were devided into experimental group (acupuncture group) and normal group (contrast group)， and the warning syncope and syncope cases were observed. RESULTS In the experimental group， there was only 1 case of warning syncope， and the contrast group 6 syncope added by 15 warning syncope. The two groups showed apparently different results (P<0.01). CONCLUSION Acupuncture can prevent syncope effectively， and it can release the patients' pain at the same time， which is worthy to be popularized and applied clinically.

Effects of Combined Acupuncture and Medication on Fat Content and Body Mass Indexes of Patients with Metabolic Syndrome

OUYANG Gang, GE Wei, XI Yang

2013, 29(1): 89-91.

[Abstract](654) [PDF 365KB](786)

Abstract:

Therapeutic Effect of Jujing Pill on 80 Cases of Oligospermia or Asthenospermia

ZHOUHua, SUNDa-lin, JINBao-fang, XUFu-song, ZHANGXin-dong

2013, 29(1): 92-93.

[Abstract](824) [PDF 297KB](621)

Abstract:
OBJECTIVE To observe the clinical efficacy of Jujing Pill in treating oligospermia or asthenospermia. METHODS 125 cases of the oligospermia or asthenospermia patients were divided into control group (40 cases) and treatment group (85cases) at random. The control group took Vit E twice a day and 100 mg each time. The treatment group were treated by Jujing Pill one dose a day. The semen volume， semen density， a and b grade sperms， total motility rate and pregnant rate of the two groups were tested 6 months before and after treatment. RESULTS 125 patients all finished the treatment. Both two groups can increase patients' semen volume， a standard sperms， forward moving sperms and total motility rate (P<0.05). The treatment group could also improve the patients' semen density remarkably (P<0.05) and was superior to control group in the improvement of semen density， a grade sperms and forward moving sperms， total motility rate and pregnant rate (P<0.05). CONCLUTION Jujing Pill can significantly improve the semen quality of the oligospermia or asthenospermia patients and be used to treat sterility.

Research Progress on Finite Element Analysis in Manual Therapy Treating Lumbar Disc Herniation

LIQing-bin, FENGYue, LUOJian, LUOCai-gui

2013, 29(1): 94-96.

[Abstract](650) [PDF 357KB](865)

Abstract:

Research Progress on Traditional Chinese Medicine Improving Endometrial Receptivity

XUEMin, SUNDa-lin, JINBao-fang

2013, 29(1): 97-100.

[Abstract](666) [PDF 415KB](670)

Abstract:

2013 Vol. 29, No. 1

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