Research Progress of Chinese Medicine in Regulating Autophagy-Related Pathways Against Lung Cancer
-
摘要: 肺癌作为全球高发病率和高死亡率的恶性肿瘤, 严重威胁着人类的生命健康。目前临床治疗肺癌主要以手术联合放疗、化疗等综合治疗为主, 可在一定程度上控制肺癌的进展, 但仍存在存活率低、生活质量差等问题。自噬是一种复杂的胞内自我降解机制, 自噬的启动与自噬相关基因蛋白及信号通路密切相关。研究表明, 合理地调控信号通路可以干预细胞自噬导致肺癌细胞死亡进而抑制肿瘤的生长。近年来, 中医药调控自噬相关信号通路抗肺癌已成为肿瘤研究领域的热点, 对近几年关于中医药调控自噬相关信号通路抗肺癌的研究进行梳理和总结, 以期为中医药防治肺癌的新药研发及临床应用提供参考依据。Abstract: As a malignant tumor with high incidence and mortality worldwide, lung cancer seriously threatens the life and health of human beings. At present, clinical treatment of lung cancer is mainly based on surgery combined with radiotherapy and chemotherapy and other comprehensive treatments, which can control the progression of lung cancer to a certain extent, but there are still problems such as low survival rate and poor quality of life. Autophagy is a complex intracellular self degradation mechanism. The occurrence of autophagy is closely related to autophagy-related gene proteins and signal pathways. Research shows that reasonable regulation of signal pathway can interfere with autophagy leading to lung cancer cell death and inhibit tumor growth. In recent years, the regulation of autophagy-related signaling pathways in Chinese medicine against lung cancer has become a hot spot in the field of oncology research. Therefore, this paper compares and summarizes the research on the regulation of autophagy-related signaling pathways in Chinese medicine against lung cancer in recent years, in order to provide a reference basis for the development of new drugs and clinical application of Chinese medicine against lung cancer.
-
Key words:
- traditional Chinese medicine /
- autophagy /
- signaling pathway /
- lung cancer
-
图 1 正虚邪滞与自噬在肺癌中的关系
Figure 1. The relationship between healthy qi deficiency and pathogenic qi stagnation and autophagy in lung cancer
表 1 中药活性成分/提取物通过自噬相关信号通路抗肺癌的作用机制
Table 1. The mechanism of action of active components/extracts of traditional Chinese medicine against lung cancer through autophagy related signaling pathways
分类 中药活性成分/提取物 模型 作用靶点 信号通路 酚类 白藜芦醇[31] A549细胞 Beclin-1↑、LC3B↑、LC3Ⅱ/LC3Ⅰ↑、NGFR↑ NGFR/AMPK/mTOR 白藜芦醇[32] A549细胞、H1299细胞 Beclin-1↑、LC3Ⅱ/LC3Ⅰ↑、p62↓、SIRT1↑、p-Akt↓、p-mTOR↓、p-p70S6K↓、p38↑ Akt/mTOR、p38/MAPK 四氢姜黄素[33] A549细胞 Beclin-1↑、LC3I/LC3Ⅱ↓、p62↓、p-mTOR↓、p-Akt↓、p-PI3K↓ PI3K/Akt/mTOR 和厚朴酚[34] A549细胞 LC3Ⅱ↑、p-mTOR↓ mTOR 黄酮类 槲皮素[35] A549细胞、H1299细胞 Beclin-1↑、LC3Ⅱ/LC3Ⅰ↑、p62↓、LC3Ⅱ↑、Atg5↑、Atg7↑、Atg12↑、SIRT1↑、p-AMPK/AMPK↑ SIRT1/AMPK 槲皮素[36] A549细胞 LC3Ⅱ/LC3Ⅰ↑、Beclin-1↑、p-AMPK/AMPK↑、p-mTOR/mTOR↓、p-S6K/S6K↓ AMPK/mTOR 木犀草素[37] A549细胞 LC3Ⅱ/LC3Ⅰ↑、p-PI3K↓、p-Akt↓、p-mTOR↓ PI3K/Akt/mTOR 萜类 鸦胆子素D[38] A549细胞、NCI-H292细胞 LC3Ⅱ/LC3Ⅰ↑、p62↓、Atg7↑、ERK↑、JNK↑、p-p38↓ ROS/MAPK 葫芦素E[39] A549细胞 Beclin-1↑、LC3Ⅱ/LC3Ⅰ↑、PI3K↓、p-PI3K↑、Akt↓、p-Akt↓、mTOR↓、p-mTOR↓ PI3K/Akt/mTOR 雷公藤甲素[40] A549细胞 LC3Ⅱ↑、p-ERK/ERK↑ ERK 苷类 旱莲苷A[41] H460细胞、H1975细胞 LC3Ⅱ/LC3Ⅰ↑、Beclin-1↑、p62/SQSTM1↑ ASK1/JNK 纤细薯蓣皂苷[42] A549细胞 Beclin-1↑、LC3Ⅱ/LC3Ⅰ↑、p62↓、WIPI1↑、p-PI3K↓、p-Akt↓、p-mTOR↓、p-AMPK↑ mTOR 延龄草提取物[43] H460细胞、PC9细胞 LC3Ⅱ↑、p62↑、p-AMPKα↑、p-mTOR↓、p-P70S6K↓、p-4EBP1↓ AMPK/mTOR 重楼皂苷I[44] H460细胞、PC9细胞 Beclin-1↑、LC3Ⅱ/LC3Ⅰ↑、p-AMPKα↓、p-mTOR↓、p-P70S6K↓、p-4EBP1↓ AMPK/mTOR 生物碱类 苦参碱[45] A549细胞 p-Akt↓、p-mTOR↓、LC3B↑ PI3K/Akt/mTOR 千金藤素[46] A549细胞 LC3↑、p-p38↑ P38 多糖类 黄芪多糖[47] A549细胞 p62↑、LC3B↓、Beclin-1↓、PI3K↓、Akt↑、mTOR↑ PI3K/Akt/mTOR 黄芪多糖[48] A549细胞 PI3K↓、LC3B↓、Akt↑ PI3K/Akt 木脂素类 五味子素A[49] A549细胞、H1299细胞、H1975细胞 LC3Ⅱ↑、p62↑、p-AMPKα-T172↑ AMPK 倍半萜类化合物 去氢木香内酯[50] A549细胞 Atg3↑、Atg5↑、LC3↑、p-Akt↓、p-mTOR↓、p-STAT3↓ Akt/mTOR/STAT3 注: ↑表示促进或上调; ↓表示抑制或下调。 
下载: 导出CSV
表 2 中药复方/制剂通过自噬相关信号通路抗肺癌的作用机制
Table 2. The mechanism of action of traditional Chinese medicine compound/preparation against lung cancer through autophagy related signaling pathways
中药复方 组成及剂量 模型 作用靶点 信号通路 沙参麦冬汤[52] 沙参、麦冬各30 g, 玉竹15 g, 生甘草6 g, 冬桑叶、天花粉、生扁豆各10 g A549细胞 Ki-67↓、p62↓、GFP-LC3↑、LC3Ⅱ/LC3I↑、p-EGFR/EGFR↓、p-MEK/MEK↓、p-ERK/ERK↓ EGFR/MEK/ERK 沙参麦冬汤[53] 北沙参、麦冬各36 g, 玉竹24 g, 天花粉、生扁豆、霜桑叶各18 g, 生甘草12 g BEAS-2B细胞 LC3Ⅱ/LC3Ⅰ↑、Beclin-1↑、mTOR↓ PI3K/Akt/mTOR 参苓白术散[55] - Lewis肺癌小鼠 TNF-α↓、IL-1β↓、IκBα↑、NF-κB↓、Atg3↑、Atg5↑、Beclin-1↑、LC3Ⅱ/LC3I↑ IκBα/NF-κB 益气扶正解毒汤[57] 黄芪、莪术、白花蛇舌草、麦冬各28 g, 黄芩22 g A549细胞 LC3Ⅱ↑、Beclin-1↑、p62↓、MAP1LC3B↑、p-AMPK/AMPK↑、p-mTOR/mTOR↓ p53/AMPK 益气扶正解毒汤[58] 黄芪、莪术、白花蛇舌草、麦冬各28 g, 黄芩22 g Lewis肺癌小鼠 Ki67↓、LC3Ⅱ/Ⅰ↑、Beclin-1↑、Atg5↑、Atg7↑、p62↓、p53↑、p-AMPK/AMPK↑、p-mTOR/mTOR↓ p53/AMPK/mTOR 百合固金汤[60] - A549细胞、H1299细胞 LC3Ⅱ↑、p62↓、p-AMPK↑、p-ULK1↑、p-70↓ AMPK/mTORC1/ULK1 芪玉三龙汤[61] 黄芪30 g, 龙葵、白花蛇舌草、薏苡仁各20 g, 天龙、地龙、泽漆、川贝母各6 g, 莪术、玉竹各10 g A549细胞、 Atg-7↑、Beclin-1↑、LC3Ⅱ/LC3I↑、p62↓、p-mTOR↓ mTOR 芪玉三龙汤[62] - A549细胞 LC3Ⅱ/LC3Ⅰ↑、Atg13↑、Beclin-1↑、Atg5↑、mTOR↓ mTOR 扶正抑瘤汤[64] 三棱、白英、白术各10 g, 莪术、龙葵各15 g, 半枝莲、白花蛇舌草、茯苓、当归各30 g, 黄芪9 g A549细胞 Beclin-1↑、LC3Ⅱ/LC3Ⅰ↑、p62↓、p-PI3K↓、p-Akt↓、p-mTOR↓、p-PI3K/PI3K↓、p-Akt/Akt↓、p-mTOR/mTOR↓ PI3K/Akt/mTOR 培元抗癌汤[65] 西洋参、黄芪、炒白术、莪术、白花蛇舌草、茯苓各15 g, 郁金、厚朴、法半夏、焦山楂各12 g Lewis肺癌小鼠 LC3Ⅱ/LC3Ⅰ↑、p-PI3K↓、p-Akt↓、p-mTOR↓ PI3K/Akt/mTOR 肺积宁方[67] 柴胡、半夏、炙甘草各10 g, 黄芩、人参、莪术、浙贝母、桔梗、茯苓各15 g, 龙骨、牡蛎、山慈姑各25 g Lewis肺癌小鼠 GFP-LC3↑、Beclin-1↑、LC3B-Ⅱ/LC3B-Ⅰ↑、Atg5↑、p-Akt↓、p-mTOR↓ PI3K/Akt/mTOR 注: ↑表示促进或上调; ↓表示抑制或下调。
下载: 导出CSV
-
[1] CHEN PX, LIU YH, WEN YK, et al. Non-small cell lung cancer in China[J]. Cancer Commun, 2022, 42(10): 937-970. doi: 10.1002/cac2.12359 [2] SUNG H, FERLAY J, SIEGEL RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71(3): 209-249. doi: 10.3322/caac.21660 [3] ETTINGER DS, WOOD DE, AISNER DL, et al. Non-small cell lung cancer, version 3.2022, NCCN clinical practice guidelines in oncology[J]. J Natl Compr Canc Netw, 2022, 20(5): 497-530. doi: 10.6004/jnccn.2022.0025 [4] ZHOU YH, MANGHWAR H, HU WM, et al. Degradation mechanism of autophagy-related proteins and research progress[J]. Int J Mol Sci, 2022, 23(13): 7301. doi: 10.3390/ijms23137301 [5] KLIONSKY DJ, PETRONI G, AMARAVADI RK, et al. Autophagy in major human diseases[J]. EMBO J, 2021, 40(19): e108863. doi: 10.15252/embj.2021108863 [6] REN YH, CAO LM, WANG LM, et al. Autophagic secretion of HMGB1 from cancer-associated fibroblasts promotes metastatic potential of non-small cell lung cancer cells via NFκB signaling[J]. Cell Death Dis, 2021, 12(10): 858. doi: 10.1038/s41419-021-04150-4 [7] 尤程程, 黄益玲. 自噬与肺癌化疗和靶向药物耐药关系的研究进展[J]. 现代肿瘤医学, 2022, 30(24): 4539-4544. doi: 10.3969/j.issn.1672-4992.2022.24.026YOU CC, HUANG YL. Advances in research on autophagy-related chemotherapeutic and targeted drug resistance in lung cancer[J]. J Mod Oncol, 2022, 30(24): 4539-4544. doi: 10.3969/j.issn.1672-4992.2022.24.026 [8] 王笑冉, 赵文霞, 苗明三. 自噬在癌症中的双重作用及中药干预研究进展[J/OL]. 中国实验方剂学杂志, 2023: 1-11. https://doi.org/10.13422/j.cnki.syfjx.20230624 .WANG XR, ZHAO WX, MIAO MS. Research pogress on dual role of autophagy in cancer and the intervention of traditional Chinese medicine[J/OL]. Chin J Exper Tradit Med Formul, 2023: 1-11.https://doi.org/10.13422/j.cnki.syfjx.20230624 .[9] DIKIC I, ELAZAR Z. Mechanism and medical implications of mammalian autophagy[J]. Nat Rev Mol Cell Biol, 2018, 19(6): 349-364. doi: 10.1038/s41580-018-0003-4 [10] ZHOU YH, MANGHWAR H, HU WM, et al. Degradation mechanism of autophagy-related proteins and research progress[J]. Int J Mol Sci, 2022, 23(13): 7301. doi: 10.3390/ijms23137301 [11] LEVINE B, KROEMER G. Biological functions of autophagy genes: A disease perspective[J]. Cell, 2019, 176(1/2): 11-42. [12] CAO WY, LI JH, YANG KP, et al. An overview of autophagy: Mechanism, regulation and research progress[J]. Bull Cancer, 2021, 108(3): 304-322. doi: 10.1016/j.bulcan.2020.11.004 [13] PATEL AS, MORSE D, CHOI AMK. Regulation and functional significance of autophagy in respiratory cell biology and disease[J]. Am J Respir Cell Mol Biol, 2013, 48(1): 1-9. doi: 10.1165/rcmb.2012-0282TR [14] YU W, ZHA WL, REN J. Exendin-4 and liraglutide attenuate glucose toxicity-induced cardiac injury through mTOR/ULK1-dependent autophagy[J]. Oxid Med Cell Longev, 2018, 2018: 5396806. [15] YU L, CHEN Y, TOOZE SA. Autophagy pathway: Cellular and molecular mechanisms[J]. Autophagy, 2018, 14(2): 207-215. doi: 10.1080/15548627.2017.1378838 [16] YU JE, KIM Y, HONG DE, et al. Bee venom triggers autophagy-induced apoptosis in human lung cancer cells via the mTOR signaling pathway[J]. J Oncol, 2022, 2022: 8916464. [17] ZHANG LX, YANG X, WU ZB, et al. TTI1 promotes non-small-cell lung cancer progression by regulating the mTOR signaling pathway[J]. Cancer Sci, 2023, 114(3): 855-869. doi: 10.1111/cas.15668 [18] HAN TY, WANG PC, WANG YN, et al. FAIM regulates autophagy through glutaminolysis in lung adenocarcinoma[J]. Autophagy, 2022, 18(6): 1416-1432. doi: 10.1080/15548627.2021.1987672 [19] CHEN LM, SONG TJ, XIAO JH, et al. Tripchlorolide induces autophagy in lung cancer cells by inhibiting the PI3K/Akt/mTOR pathway and improves cisplatin sensitivity in A549/DDP cells[J]. Oncotarget, 2017, 8(38): 63911-63922. doi: 10.18632/oncotarget.19201 [20] TAN AC. Targeting the PI3K/Akt/mTOR pathway in non-small cell lung cancer (NSCLC)[J]. Thorac Cancer, 2020, 11(3): 511-518. doi: 10.1111/1759-7714.13328 [21] DANESH PAZHOOH R, RAHNAMAY FARNOOD P, ASEMI Z, et al. mTOR pathway and DNA damage response: A therapeutic strategy in cancer therapy[J]. DNA Repair, 2021, 104: 103142. doi: 10.1016/j.dnarep.2021.103142 [22] SHAO SL, ZHUANG XB, ZHANG L, et al. Antidepressants fluoxetine mediates endoplasmic reticulum stress and autophagy of non-small cell lung cancer cells through the ATF4-AKT-mTOR signaling pathway[J]. Front Pharmacol, 2022, 13: 904701. doi: 10.3389/fphar.2022.904701 [23] NIE YL, MEI X, CAO FJ, et al. BDH2 promotes apoptosis and autophagy of lung adenocarcinoma cells via Akt/mTOR pathway[J]. Gen Physiol Biophys, 2022, 41(2): 115-122. [24] 念家云, 王笑民, 富琦, 等. 基于PI3K/Akt/mTOR信号传导通路的抗癌中药单体的研究概况[J]. 中国药房, 2019, 30(20): 2870-2875. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYA201920026.htmNIAN JY, WANG XM, FU Q, et al. General situation of research on anti-cancer traditional Chinese medicine monomer based on PI3K/Akt/mTOR signal transduction pathway[J]. China Pharm, 2019, 30(20): 2870-2875. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYA201920026.htm [25] LIU GB, PEI F, YANG FQ, et al. Role of autophagy and apoptosis in non-small-cell lung cancer[J]. Int J Mol Sci, 2017, 18(2): 367. doi: 10.3390/ijms18020367 [26] CAI ZJ, TCHOU-WONG KM, ROM WN. NF-κB in lung tumorigenesis[J]. Cancers, 2011, 3(4): 4258-4268. doi: 10.3390/cancers3044258 [27] 夏茂, 孟刚, 董杰. NF-κB信号通路抑制剂与溶瘤麻疹病毒疫苗株协同抗肺癌的作用及机制[J]. 中国肺癌杂志, 2021, 24(4): 245-253. https://www.cnki.com.cn/Article/CJFDTOTAL-FAIZ202104004.htmXIA M, MENG G, DONG J. Synergistic effect of NF-κB signaling pathway inhibitor and oncolytic measles virus vaccine strain against lung cancer and underlying mechanisms[J]. Chin J Lung Cancer, 2021, 24(4): 245-253. https://www.cnki.com.cn/Article/CJFDTOTAL-FAIZ202104004.htm [28] 邓小垭, 罗勤利, 董飞, 等. Tristetraprolin通过NF-κB通路抑制肺腺癌细胞自噬[J]. 南方医科大学学报, 2019, 39(3): 313-319. https://www.cnki.com.cn/Article/CJFDTOTAL-DYJD201903010.htmDENG XY, LUO QL, DONG F, et al. Tristetraprolin inhibits autophagy in cultured lung cancer cells via the nuclear factor - κB pathway[J]. J South Med Univ, 2019, 39(3): 313-319. https://www.cnki.com.cn/Article/CJFDTOTAL-DYJD201903010.htm [29] LIU GB, PEI F, YANG FQ, et al. Role of autophagy and apoptosis in non-small-cell lung cancer[J]. Int J Mol Sci, 2017, 18(2): 367. doi: 10.3390/ijms18020367 [30] ZHU YJ, JIANG X, DING ZY, et al. Interleukin 7 inhibit autophagy via P53 regulated AMPK/mTOR signaling pathway in non-small cell lung cancer[J]. Sci Rep, 2022, 12(1): 11208. doi: 10.1038/s41598-022-14742-5 [31] LI JQ, FAN YM, ZHANG Y, et al. Resveratrol induces autophagy and apoptosis in non-small-cell lung cancer cells by activating the NGFR-AMPK-mTOR pathway[J]. Nutrients, 2022, 14(12): 2413. doi: 10.3390/nu14122413 [32] WANG J, LI JM, CAO NQ, et al. Resveratrol, an activator of SIRT1, induces protective autophagy in non-small-cell lung cancer via inhibiting Akt/mTOR and activating p38-MAPK[J]. Onco Targets Ther, 2018, 11: 7777-7786. doi: 10.2147/OTT.S159095 [33] SONG GQ, LU HH, CHEN F, et al. Tetrahydrocurcumin-induced autophagy via suppression of PI3K/Akt/mTOR in non-small cell lung carcinoma cells[J]. Mol Med Rep, 2018, 17(4): 5964-5969. [34] 罗敏, 缪萍, 吴爱祥, 等. 和厚朴酚通过mTOR信号通路诱导肺癌A549细胞自噬[J]. 中国病理生理杂志, 2019, 35(7): 1195-1198. doi: 10.3969/j.issn.1000-4718.2019.07.007LUO M, MIAO P, WU AX, et al. Honokiol induces autophagy by inhibiting mTOR signaling pathway in lung cancer A549 cells[J]. Chin J Pathophysiol, 2019, 35(7): 1195-1198. doi: 10.3969/j.issn.1000-4718.2019.07.007 [35] GUO HJ, DING H, TANG X, et al. Quercetin induces pro-apoptotic autophagy via SIRT1/AMPK signaling pathway in human lung cancer cell lines A549 and H1299 in vitro[J]. Thorac Cancer, 2021, 12(9): 1415-1422. doi: 10.1111/1759-7714.13925 [36] 文兰香, 覃世运, 陈丽君. 槲皮素调控AMPK/mTOR通路对肺癌A549细胞自噬的影响[J]. 中国免疫学杂志, 2020, 36(19): 2375-2379. doi: 10.3969/j.issn.1000-484X.2020.19.019WEN LX, QIN SY, CHEN LJ. Effect of quercetin on autophagy of lung cancer A549 cells by regulating AMPK/mTOR pathway[J]. Chin J Immunol, 2020, 36(19): 2375-2379. doi: 10.3969/j.issn.1000-484X.2020.19.019 [37] 杨朋, 钱军, 陈文飞, 等. 木犀草素对人非小细胞肺癌A549细胞凋亡及自噬的影响[J]. 中成药, 2022, 44(8): 2667-2671. doi: 10.3969/j.issn.1001-1528.2022.08.045YANG P, QIAN J, CHEN WF, et al. Effects of luteolin on apoptosis and autophagy of human non-small cell lung cancer A549 cells[J]. Chin Tradit Pat Med, 2022, 44(8): 2667-2671. doi: 10.3969/j.issn.1001-1528.2022.08.045 [38] FAN JJ, REN DM, WANG JX, et al. Bruceine D induces lung cancer cell apoptosis and autophagy via the ROS/MAPK signaling pathway in vitro and in vivo[J]. Cell Death Dis, 2020, 11(2): 126. doi: 10.1038/s41419-020-2317-3 [39] ZHANG JF, ARAY B, ZHANG Y, et al. Synergistic effect of cucurbitacin E and myricetin on Anti-Non-Small cell lung cancer: Molecular mechanism and therapeutic potential[J]. Phytomedicine, 2023, 111: 154619. doi: 10.1016/j.phymed.2022.154619 [40] 王伟, 王坤. 雷公藤甲素通过ERK通路诱导肺癌A549细胞自噬[J]. 中国病理生理杂志, 2016, 32(9): 1551-1555. doi: 10.3969/j.issn.1000-4718.2016.09.003WANG W, WANG K. Tripchlorolide activates p-ERK and induces autophagy in lung cancer A549 cells[J]. Chin J Pathophysiol, 2016, 32(9): 1551-1555. doi: 10.3969/j.issn.1000-4718.2016.09.003 [41] HAN J, LV W, SHENG HX, et al. Ecliptasaponin A induces apoptosis through the activation of ASK1/JNK pathway and autophagy in human lung cancer cells[J]. Ann Transl Med, 2019, 7(20): 539. doi: 10.21037/atm.2019.10.07 [42] LI YM, LIU H, LIU XX, et al. Gracillin shows potential efficacy against non-small cell lung cancer through inhibiting the mTOR pathway[J]. Front Oncol, 2022, 12: 851300. doi: 10.3389/fonc.2022.851300 [43] 金鑫, 谭苗, 任宇亮, 等. 一种延龄草提取物激活AMPK诱导非小细胞肺癌自噬的作用机制[J]. 湖北医药学院学报, 2022, 41(4): 350-356, 381, 322, 441. https://www.cnki.com.cn/Article/CJFDTOTAL-YYYX202204005.htmJIN X, TAN M, REN YL, et al. Mechanism of Trillium extract activating AMPK to induce autophagy in non-small cell lung cancer[J]. J Hubei Univ Med, 2022, 41(4): 350-356, 381, 322, 441. https://www.cnki.com.cn/Article/CJFDTOTAL-YYYX202204005.htm [44] 彭鹏, 金鑫, 申杰, 等. 重楼皂苷Ⅰ通过激活AMPK信号通路诱导非小细胞肺癌自噬的作用机制[J]. 湖北医药学院学报, 2021, 40(1): 1-6, 15, 113. doi: 10.13819/j.issn.2096-708X.2021.01.001PENG P, JIN X, SHEN J, et al. The mechanism of polyphyllinⅠinducing autophagy in non-small cell lung cancer by activating AMPK signaling pathway[J]. J Hubei Univ Med, 2021, 40(1): 1-6, 15, 113. doi: 10.13819/j.issn.2096-708X.2021.01.001 [45] 郝艳梅, 殷红梅, 朱超莽, 等. 苦参碱通过抑制PI3K/AKT/mTOR通路促进非小细胞肺癌A549细胞的自噬和凋亡[J]. 南方医科大学学报, 2019, 39(7): 760-765. https://www.cnki.com.cn/Article/CJFDTOTAL-DYJD201907003.htmHAO YM, YIN HM, ZHU CM, et al. Matrine inhibits proliferation and promotes autophagy and apoptosis in non-small cell lung cancer cells by deactivating PI3K/AKT/mTOR pathway[J]. J South Med Univ, 2019, 39(7): 760-765. https://www.cnki.com.cn/Article/CJFDTOTAL-DYJD201907003.htm [46] LI G, QIAO KS, XU XD, et al. Cepharanthine regulates autophagy via activating the p38 signaling pathway in lung adenocarcinoma cells[J]. Anticancer Agents Med Chem, 2022, 22(8): 1523-1529. doi: 10.2174/1871520621666210903163407 [47] 杨琪, 段俊颖, 王雪林, 等. 黄芪多糖对肺癌A549细胞自噬的作用及机制研究[J]. 中国临床药理学杂志, 2022, 38(12): 1329-1333. doi: 10.13699/j.cnki.1001-6821.2022.12.008YANG Q, DUAN JY, WANG XL, et al. Effect and mechanism of Astragalus polysaccharides on autophagy in lung cancer A549 cells[J]. Chin J Clin Pharmacol, 2022, 38(12): 1329-1333. doi: 10.13699/j.cnki.1001-6821.2022.12.008 [48] 王雪林, 李杨, 刘丹, 等. 黄芪多糖对黄嘌呤氧化酶诱导的肺癌A549细胞自噬及PI3K/AKT信号通路的影响[J]. 中国药理学通报, 2019, 35(12): 1676-1680. doi: 10.3969/j.issn.1001-1978.2019.12.011WANG XL, LI Y, LIU D, et al. Effect of astragalus polysaccharide on autophagy and PI3K/AKT signaling pathway in lung cancer A549 cells induced by xanthine oxidase[J]. Chin Pharmacol Bull, 2019, 35(12): 1676-1680. doi: 10.3969/j.issn.1001-1978.2019.12.011 [49] ZHU LH, WANG Y, LV W, et al. Schizandrin A can inhibit non-small cell lung cancer cell proliferation by inducing cell cycle arrest, apoptosis and autophagy[J]. Int J Mol Med, 2021, 48(6): 214. doi: 10.3892/ijmm.2021.5047 [50] 田颖颖, 李依林, 田时秋, 等. 去氢木香内酯激活凋亡与自噬抑制人肺癌A549细胞生长[J]. 中国实验方剂学杂志, 2023, 29(2): 73-80. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX202302010.htmTIAN YY, LI YL, TIAN SQ, et al. Dehydrocostus lactone inhibits growth of human lung cancer A549 cells through activation of apoptosis and autophagy[J]. Chin J Exp Tradit Med Formulae, 2023, 29(2): 73-80. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX202302010.htm [51] 黄秋华, 梁耀君, 陈楠, 等. 沙参麦冬汤对非小细胞肺癌患者肿瘤标志物水平的影响[J]. 现代医学与健康研究电子杂志, 2021, 5(12): 87-89. https://www.cnki.com.cn/Article/CJFDTOTAL-XYJD202112035.htmHUANG QH, LIANG YJ, CHEN N, et al. Effect of Shashen Maidong Decoction on tumor markers in patients with non-small cell lung cancer[J]. Mod Med Heath Res Electron J, 2021, 5(12): 87-89. https://www.cnki.com.cn/Article/CJFDTOTAL-XYJD202112035.htm [52] 郭晓黎, 任小宁, 樊一波, 等. 沙参麦冬汤通过调节EGFR/MEK/ERK信号通路对非小细胞肺癌细胞增殖、凋亡和自噬的影响[J]. 中国药师, 2022, 25(10): 1707-1713. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYSG202210003.htmGUO XL, REN XN, FAN YB, et al. Impacts of Radix Ophiopogon decoction on the proliferation, apoptosis and autophagy of non-small cell lung cancer cells by regulating EGFR/MEK/ERK signaling pathway[J]. China Pharm, 2022, 25(10): 1707-1713. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYSG202210003.htm [53] 高启龙, 范修琦, 崔姗姗, 等. 基于PI3K/AKT/mTOR通路探讨沙参麦冬汤调控自噬干预BEAS-2B细胞恶性转化[J]. 中华中医药杂志, 2022, 37(1): 508-512. https://www.cnki.com.cn/Article/CJFDTOTAL-BXYY202201104.htmGAO QL, FAN XQ, CUI SS, et al. Study on the mechanism of Shashen Maidong Decoction regulated autophagy to inhibite the malignant transformation of BEAS-2B cells based on PI3K/AKT/mTOR signaling pathway[J]. China J Tradit Chin Med Pharm, 2022, 37(1): 508-512. https://www.cnki.com.cn/Article/CJFDTOTAL-BXYY202201104.htm [54] XU WJ, HAN QR, LIANG ST, et al. Modified Shenlingbaizhu Decoction reduces intestinal adenoma formation in adenomatous polyposis coli multiple intestinal neoplasia mice by suppression of hypoxia-inducible factor 1α-induced CD4+CD25+ forkhead box P3 regulatory T cells[J]. J Tradit Chin Med, 2018, 38(1): 22-32. [55] 张云亭, 刘羽茜, 蒋宗蓥, 等. 参苓白术散通过IκBα/NF-κB通路调控Lewis肺癌小鼠肿瘤自噬的研究[J]. 世界科学技术-中医药现代化, 2022, 24(4): 1487-1494. https://www.cnki.com.cn/Article/CJFDTOTAL-SJKX202204018.htmZHANG YT, LIU YX, JIANG ZY, et al. Study of IκBα/NF-κB pathway about regulation of autophagy in lewis lung cancer mice through reinforcing shenlingbaizhu powder[J]. Mod Tradit Chin Med Mater Med World Sci Technol, 2022, 24(4): 1487-1494. https://www.cnki.com.cn/Article/CJFDTOTAL-SJKX202204018.htm [56] 米雪, 亓润智, 花宝金. 肺癌中医证治规律分析探讨[J]. 时珍国医国药, 2020, 31(6): 1510-1512. https://www.cnki.com.cn/Article/CJFDTOTAL-SZGY202006079.htmMI X, QI RZ, HUA BJ. Analysis and discussion on the law of TCM syndrome differentiation and treatment of lung cancer[J]. Lishizhen Med Mater Med Res, 2020, 31(6): 1510-1512. https://www.cnki.com.cn/Article/CJFDTOTAL-SZGY202006079.htm [57] 吴俏兰, 宋婷, 陈泽涛, 等. 基于p53/AMPK信号通路观察益气扶正解毒汤对A549细胞自噬水平和生长的影响[J]. 中国实验方剂学杂志, 2021, 27(22): 65-75. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX202122010.htmWU QL, SONG T, CHEN ZT, et al. Effect of Yiqi fuzheng Jiedu Decoction on autophagy and growth of A549 cells: An exploration based on p53/AMPK signaling pathway[J]. Chin J Exp Tradit Med Formulae, 2021, 27(22): 65-75. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX202122010.htm [58] 吴俏兰, 宋婷, 纪凌云, 等. 基于p53/AMPK/mTOR信号通路调控细胞自噬探讨益气扶正解毒汤抑制Lewis肺癌小鼠皮下肿瘤生长的作用机制[J]. 中药新药与临床药理, 2022, 33(5): 565-573. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYXY202205001.htmWU QL, SONG T, JI LY, et al. Study on the mechanism of Yiqi fuzheng Jiedu Decoction inhibiting subcutaneous tumor growth in mice with lewis lung cancer through regulating autophagy by p53/AMPK/mTOR pathway[J]. Tradit Chin Drug Res Clin Pharmacol, 2022, 33(5): 565-573. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYXY202205001.htm [59] LI TM, YU YH, TSAI FJ, et al. Characteristics of Chinese herbal medicine usage and its effect on survival of lung cancer patients in Taiwan[J]. J Ethnopharmacol, 2018, 213: 92-100. [60] WU QH, LI D, SUN TL, et al. Bai-He-Gu-Jin-Tang formula suppresses lung cancer via AKT/GSK3β/β-catenin and induces autophagy via the AMPK/mTORC1/ULK1 signaling pathway[J]. J Cancer, 2021, 12(21): 6576-6587. [61] GAO YT, WANG XH, YANG QJ, et al. Qiyusanlong formula induces autophagy in non-small-cell lung cancer cells and xenografts through the mTOR signaling pathway[J]. Evid Based Complement Alternat Med, 2021, 2021: 5575453. [62] 高雅婷, 王心恒, 王小乐, 等. 芪玉三龙汤调节mTOR/Beclin1/LC3信号轴相关分子的表达诱导肺癌A549细胞自噬[J]. 中国实验方剂学杂志, 2021, 27(1): 98-104. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX202101013.htmGAO YT, WANG XH, WANG XL, et al. Effect of Qiyu Sanlong decoction in inducing autophagy of A549 cells by regulating molecular expression related to signal axis of mTOR-Beclin1-LC3[J]. Chin J Exp Tradit Med Formulae, 2021, 27(1): 98-104. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX202101013.htm [63] FU W, HONG ZM, YOU XJ, et al. Enhancement of anticancer activity of docetaxel by combination with Fuzheng Yiliu Decoction in a mouse model of castration-resistant prostate cancer[J]. Biomed Pharmacother, 2019, 118: 109374. [64] 孟东雪, 郭玉荣, 罗斌军, 等. 扶正抑瘤汤通过PI3K/Akt/mTOR信号通路抑制非小细胞肺癌增殖、凋亡及自噬[J]. 中国癌症防治杂志, 2021, 13(2): 177-182. https://www.cnki.com.cn/Article/CJFDTOTAL-ZAZF202102012.htmMENG DX, GUO YR, LUO BJ, et al. Fuzheng Yiliu Decoction inhibits the proliferation, apoptosis and autophagy of non-small cell lung cancer through PI3K/Akt/mTOR signaling pathway[J]. Chin J Oncol Prev Treat, 2021, 13(2): 177-182. https://www.cnki.com.cn/Article/CJFDTOTAL-ZAZF202102012.htm [65] 司海龙, 陈玉, 苟涛, 等. 培元抗癌汤通过PI3K-AKT-mTOR信号通路调节Lewis肺癌自噬抑制肿瘤生长和转移的实验研究[J]. 北京中医药大学学报, 2021, 44(8): 722-728. https://www.cnki.com.cn/Article/CJFDTOTAL-JZYB202108008.htmSI HL, CHEN Y, GOU T, et al. Effect of Peiyuan Kang'ai Decoction in regulating autophagy of Lewis lung cancer through PI3K-AKT-mTOR pathway to inhibit tumor growth and metastasis[J]. J Beijing Univ Tradit Chin Med, 2021, 44(8): 722-728. https://www.cnki.com.cn/Article/CJFDTOTAL-JZYB202108008.htm [66] ZHOU LJ, PAN YZ, XING YQ, et al. Effects of Feijining Decoction on vascular endothelial growth factor protein expression and changes of T cell subsets in Lewis lung carcinoma-bearing mice[J]. Biomed Rep, 2015, 3(3): 403-407. [67] 周立江, 潘玉真, 殷东风. 肺积宁方影响Lewis肺癌细胞增殖和自噬的作用机制研究[J]. 中华中医药学刊, 2016, 34(7): 1739-1742. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYHS201607059.htmZHOU LJ, PAN YZ, YIN DF. Feijining Decoction on proliferation and autophagy in lewis cells[J]. Chin Arch Tradit Chin Med, 2016, 34(7): 1739-1742. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYHS201607059.htm [68] 石伯伦, 胡佳奇, 贺忠宁, 等. 花宝金教授运用调气解毒学说辨治肺癌的思路与方法[J]. 世界中医药, 2023, 18(2): 243-248. https://www.cnki.com.cn/Article/CJFDTOTAL-SJZA202302016.htmSHI BL, HU JQ, HE ZN, et al. Professor Hua Baojin's thinking and method of treating lung cancer by regulating qi and detoxifying theory[J]. World Chin Med, 2023, 18(2): 243-248. https://www.cnki.com.cn/Article/CJFDTOTAL-SJZA202302016.htm [69] 张欣, 白妍, 陈宏. 益气活血法干预肺癌作用机制的研究进展[J]. 中国中医药科技, 2023, 30(2): 419-421. https://www.cnki.com.cn/Article/CJFDTOTAL-TJYY202302085.htmZHANG X, BAI Y, CHEN H. Research progress on the mechanism of Yiqi Huoxue method in intervening lung cancer[J]. Chin J Tradit Med Sci Technol, 2023, 30(2): 419-421. https://www.cnki.com.cn/Article/CJFDTOTAL-TJYY202302085.htm [70] 许越淇, 莫丽莎, 柯诗文, 等. 国医大师洪广祥"以补助攻, 留人治病"原则辨治晚期肺癌经验[J]. 中华中医药杂志, 2023, 38(3): 1078-1081. https://www.cnki.com.cn/Article/CJFDTOTAL-BXYY202303026.htmXU YQ, MO LS, KE SW, et al. TCM master HONG Guang-Xiang's experience in the treatment of advanced lung cancer under the principle of 'attacking with subsidy and leaving people to cure'[J]. China J Tradit Chin Med Pharm, 2023, 38(3): 1078-1081. https://www.cnki.com.cn/Article/CJFDTOTAL-BXYY202303026.htm [71] 高维, 郭蓉娟, 王永炎. 论七情致病"虚气留滞"病因病机新认识[J]. 环球中医药, 2019, 12(10): 1490-1494. https://www.cnki.com.cn/Article/CJFDTOTAL-HQZY201910007.htmGAO W, GUO RJ, WANG YY. New understanding of the etiology and pathogenesis of deficient-qi stagnation of 7 emotions pathopoiesia[J]. Glob Tradit Chin Med, 2019, 12(10): 1490-1494. https://www.cnki.com.cn/Article/CJFDTOTAL-HQZY201910007.htm [72] 俞赟丰, 唐佩, 周曼丽, 等. 基于"正虚邪滞"理论探讨肺癌自噬机制及中药干预进展[J]. 中国实验方剂学杂志, 2023, 29(18): 190-197. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX202318023.htmYU YF, TANG P, ZHOU ML, et al. Chinese medicine intervention on autophagy in lung cancer from theory of healthy qi deficiency and pathogenic qi stagnation: A review[J]. Chin J Exp Tradit Med Formulae, 2023, 29(18): 190-197. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX202318023.htm -
点击查看大图
图(1) / 表(2)
计量
- 文章访问数: 208
- HTML全文浏览量: 24
- PDF下载量: 21
- 被引次数: 0
