Euryale Ferox Petioles and Pedicels Polysaccharide Promoted Cell Apoptosis of Murine Lung Cancer LLC Cells by Inducing Cellular Infiltration of CD4+T, CD8+T and NK Cells
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摘要:
目的 探究芡茎多糖(EFPP)对肺癌LLC细胞异位移植瘤模型的影响。 方法 40只雄性C57BL/6小鼠随机分为4组, 分别为空白对照组(Control)、芡茎多糖低、高剂量组(100、200 mg·kg-1)、5-氟脲嘧啶组(5-Fu 20 mg·kg-1), 每组10只; 建立异位移植瘤模型后, 空白对照组灌胃PBS，每日1次, 给药组灌胃芡茎多糖，每日1次, 阳性药3 d注射1次, 总共给药15 d, 最后1次给药第2天处死全部小鼠。取各组肿瘤样品, 称量体积与瘤质量; 采用苏木精-伊红(HE)染色、流式细胞术、TUNEL凋亡染色以及Western blot法对芡茎多糖进行药效学检测。 结果 与空白对照相比, 芡茎多糖可以抑制LLC细胞的体内增殖, 增加CD3+CD4+T、CD3+CD8+T和CD3-NK1.1+细胞水平。同时, 芡茎多糖上调活化的半胱氨酸天冬氨酸蛋白酶3(Cleaved-caspase3)、B细胞白血病-淋巴瘤-2相关X蛋白(Bax)表达, 下调β细胞淋巴瘤/白血病基因2(Bcl2)的表达。 结论 芡茎多糖可以通过增加淋巴细胞亚群的浸润, 诱导Caspase3凋亡通路开启, 从而促进LLC细胞凋亡，为芡茎多糖抗肿瘤提供科学依据, 亦为芡资源的综合利用提供参考。 Abstract:OBJECTIVE Aimed to examine the effect of Euryale ferox petioles and pedicels polysaccharide (EFPP) on LLC cell allograft model of lung cancer. METHODS Forty male C57BL/6 rats were randomly divided into 4 groups (n=10/group), namely control group, EFPP low dose group (100 mg·kg-1 EFPP), EFPP high dose group (200 mg·kg-1 EFPP), 5-Fu(20 mg·kg-15-Fu). Different doses of EFPP were orally administered once daily for fifteen consecutive days, and the positive drug was injected once every 3 days after the ectopic xenograft tumor model was established. Rats in control group received PBS treatment by gavage. All mice were sacrificed on the second day of the last administration. Tumor samples were taken from each group, and the volume and tumor weight were weighed. Hematoxylin and eosin staining, flow cytometry, TUNEL apoptosis staining and Western blot were used to detect the pharmacodynamics of EFPP. RESULTS The data showed that EFPP could inhibit the proliferation of LLC cells in vivo. In addition, EFPP increased the levels of CD3+CD4+T, CD3+CD8+T and CD3-NK1.1+cells. At the same time, EFPP up-regulated the expression of proteins related to the Caspase3 apoptosis pathway. CONCLUSION EFPP can promote LLC cell apoptosis by increasing the infiltration of lymphocyte subsets, up-regulating the expression of cleaved-caspase3 and Bax, and down-regulating the expression of Bcl2. This study provided the scientific basis for the anti-tumor application of EFPP and utilization of euryale ferox stem resources. -
图 1 芡茎多糖对荷瘤小鼠的治疗作用
注: 与空白对照组相比, **P < 0.01。x±s, n=10。
Figure 1. Effect of EFPP on lung cancer mice
图 2 芡茎多糖对荷瘤小鼠CD3+CD4+T细胞的影响
注: 与空白对照组相比, **P < 0.01。x±s, n=3。
Figure 2. Effect of EFPP on helper T cells
图 3 芡茎多糖对荷瘤小鼠CD3+CD8+T细胞的影响
注: 与空白对照组相比, *P < 0.05, **P < 0.01。x±s, n=3。
Figure 3. Effect of EFPP on cytotoxicity T cells
图 4 芡茎多糖对荷瘤小鼠天然杀伤细胞的影响
注: 与空白对照组相比, **P < 0.01。x±s, n=3
Figure 4. Effect of EFPP on natural killer cells
图 5 芡茎多糖对荷瘤小鼠细胞凋亡的影响
注: 与空白对照组相比, **P < 0.01。x±s, n=3。
Figure 5. Effect of EFPP on LLC cell apoptosis in mice
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