宁泌泰胶囊改善慢性前列腺炎引发疼痛的研究

陆尚; 王倩; 吕志刚

doi:10.14148/j.issn.1672-0482.2023.0359

宁泌泰胶囊改善慢性前列腺炎引发疼痛的研究

doi: 10.14148/j.issn.1672-0482.2023.0359

南京中医药大学药学院/针药结合重点实验室, 江苏南京 210023

基金项目:

国家自然科学基金面上项目 81673412

江苏省科技项目 BE2019618

详细信息

作者简介:
陆尚, 男, 硕士研究生, E-mail: 1449720479@qq.com

通讯作者:
吕志刚, 男, 教授, 主要从事神经药理学研究, E-mail: amen0614@126.com

中图分类号: R285.5
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- 被引次数: 0
出版历程
- 收稿日期: 2022-10-16
- 网络出版日期: 2023-04-17
- 发布日期: 2023-04-10

Study on Ningmitai Capsule to Ameliorate Pain Caused by Chronc Prostatitis

School of Pharmacy/Key Laboratory of Combination of Acupuncture and Medicine, Nanjing 210023, China

摘要

摘要: 目的研究宁泌泰(NMT)胶囊改善慢性前列腺炎引发疼痛的作用及潜在机制。方法将50只雄性C57BL/6小鼠随机分为空白组、模型组、NMT低剂量组、NMT中剂量组、NMT高剂量组, 每组10只。造模后低、中、高剂量组分别灌胃给药6、12、18 g·kg^-1NMT溶液, 空白组、模型组灌胃等量的生理盐水。采用热辐射甩尾法和热板法进行行为学检测; 免疫组化法检测各组前列腺神经胶质纤维酸性蛋白(GFAP)以及肿瘤坏死因子-α(TNF-α)的含量; qPCR法检测前列腺组织中TNF-α、IL-6和IL-1β mRNA的相对表达量; ELISA法检测前列腺组织中IL-6、IL-1β、TNF-α表达水平; Western blot法检测p-ERK/ERK、p-JNK/JNK、p-p38MAPK/p38MAPK蛋白表达水平。结果与模型组比较, NMT低、中、高3个剂量组小鼠的热板法和甩尾法所测定痛阈值均显著提高(P < 0.01，P < 0.001), 前列腺组织中GFAP和TNF-α表达量明显降低(P < 0.05)，TNF-α、IL-6和IL-1β的mRNA水平均显著降低(P < 0.05，P < 0.01，P < 0.001), p-ERK/ERK、p-JNK/JNK、p-p38MAPK/p38MAPK蛋白水平显著降低(P < 0.05，P < 0.01)。结论 NMT胶囊可能通过抑制炎性介质的释放和星形胶质细胞的激活, 发挥对慢性前列腺炎引起小鼠疼痛的抑制作用。
- 宁泌泰胶囊 /
- 慢性前列腺炎 /
- 疼痛 /
- 镇痛机制
Abstract: OBJECTIVE To explore the effect and potential mechanism of Ningmitai (NMT) capsule on pain caused by prostatitis. METHODS 50 male C57BL/6 mice were randomly divided into 5 groups: control group, model group, NMT low dose group, NMT middle dose group, and NMT high dose group. NMT groups were given 6, 12, 18 g·kg^-1 NMT solution, respectively, by gavage after modeling. The control and model groups were intragastrically administered the same amount of normal saline. Behavioral assessment was performed using thermal radiation tail flick test and hot plate test; glial fibrillary acidic protein (GFAP) and tumor necrosis factor-α (TNF-α) contents in prostate in each group were detected by Immunohistochemistry; TNF-α, IL-6 and IL-1β mRNA levels in prostate tissue were measured by qPCR; ELISA technology was applied to determine prostate TNF-α, IL-6 and IL-1β expression; protein levels of p-ERK/ERK, p-JNK/JNK and p-P38MAPK/P38MAPK were detected by Western blot. RESULTS Compared with the model group, the pain thresholds of the hot plate and tail flick in NMT low dose, middle dose and high dose groups were significantly increased (P < 0.01, P < 0.001); GFAP and TNF-α contents were significantly decreased (P < 0.05); TNF-α, IL-6 and IL-1β mRNA levels were significantly decreased (P < 0.05, P < 0.01, P < 0.001); expression levels of p-ERK/ERK, p-JNK/JNK and p-P38MAPK/P38MAPK were significantly reduced (P < 0.05, P < 0.01). CONCLUSION NMT capsule shows a certain analgesic effect on the pain caused by chronic prostatitis in mice, which is associated with inhibiting inflammatory mediators and deactivating astrocytes.
- Ningmitai capsule /
- chronic prostatitis /
- pain /
- analgesic mechanism

HTML全文

图 1 小鼠前列腺组织染色图(HE，×40)

Figure 1. Picture of mouse prostate tissue (HE, ×40)

下载: 全尺寸图片幻灯片

图 2 各组小鼠前列腺中GFAP和TNF-α表达的免疫组化结果

注: 与空白组比较, ^*P < 0.05;与模型组比较, ^#P < 0.05。

Figure 2. Immunohistochemistry of GFAP and TNF-α expression in prostate of each group

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图 3 各组小鼠前列腺组织中相关基因mRNA水平比较

注: 与空白组比较, ^**P < 0.01，^***P < 0.001;与模型组比较, ^#P < 0.05，^##P < 0.01，^###P < 0.001。

Figure 3. mRNA levels of related genes in prostate tissue of mice in each group

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图 4 各组小鼠前列腺组织中IL-6、IL-1β、TNF-α表达水平的比较

注: 与空白组比较, ^*P < 0.05，^**P < 0.01，^***P < 0.001;与模型组比较, ^#P < 0.05，^##P < 0.01。

Figure 4. Expression levels of IL-6, IL-1β and TNF-α in each group

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图 5 各组小鼠前列腺组织ERK蛋白、JNK/p38MAPK信号通路相关蛋白表达的比较

注: 与空白组比较, ^***P < 0.001;与模型组比较, ^#P < 0.05，^##P < 0.01。

Figure 5. Expression levels of ERK and JNK/p38MAPK signal pathway-related protein in prostate tissue in each group

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表 1 引物序列

Table 1. Primer sequences

基因	上游引物(5'→3')	下游引物(5'→3')
GAPDH	ACCACAGTCCATGCCATCAC	TCCACCACCCTGTTGCTGTA
IL-6	CCCTACTTCACAAGTCCGGAGAGGAGA	GGTAGCATCCATCATTTCTTTGTATCTCT
IL-1β	CCTGTGTCTTTCCCGTGGACCTTCCAGG	CATCATCCCATGAGTCACAGAGGATGGG
TNF-α	GGAACTGGCAGAAGAGGCACTCCCC	GGCCATTTGGGAACTTCTCATCCCTTTG

下载: 导出CSV

表 2 各组小鼠热板实验疼痛阈值比较(x±s, s，n=10)

Table 2. The pain thresholds of hot plate experiment in each group(x±s, s, n=10)

组别	d0	d3	d6	d9	d12
空白组	19.44±1.38	19.78±1.78	18.57±2.21	17.89±1.87	19.40±1.62
模型组	13.66±1.56^***	12.24±1.50^**	13.05±0.75^**	13.48±1.24^***	13.54±1.59^**
NMT低剂量组	12.94±1.13	12.12±0.78	15.64±1.29^#	15.88±1.45^#	17.10±0.87^##
NMT中剂量组	11.68±0.73	13.12±0.82	16.60±0.14^###	18.16±0.59^###	19.70±0.43^###
NMT高剂量组	12.54±0.82	12.32±1.05	16.44±0.87^##	17.28±0.77^###	18.64±0.94^###
注: 与空白组比较, ^{* }P < 0.01，^{ * *}P < 0.001;与模型组比较, ^#P < 0.05，^##P < 0.01，^###P < 0.001。

下载: 导出CSV

表 3 各组小鼠甩尾实验疼痛阈值比较(x±s, s，n=10)

Table 3. The pain thresholds of tail flick experiment in each group (x±s, s, n=10)

组别	d0	d3	d6	d9	d12
空白组	7.29±0.93	7.07±0.82	7.11±0.96	8.11±1.29	8.84±1.74
模型组	3.18±0.43^**	4.16±1.08^***	3.44±0.71^***	3.20±1.15^**	3.84±1.04^**
NMT低剂量组	3.88±0.86	4.60±0.58	4.32±0.57	5.52±1.10^##	6.76±1.06^###
NMT中剂量组	3.76±0.54	4.60±0.58	5.60±0.64^##	6.68±0.43^###	8.78±0.27^###
NMT高剂量组	3.82±0.81	4.68±0.64	4.54±0.49	5.52±1.11^##	8.16±0.56^###
注: 与空白组比较, ^{* }P < 0.01，^{ * *}P < 0.001;与模型组比较, ^##P < 0.01，^###P < 0.001。

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参考文献(10)

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