HS-SPME-GC-MS结合化学计量法分析刺五加不同部位的挥发性成分

何嘉伟; 江汉美; 黄振阳; 曾格格; 戴全武; 刘天琪; 韩蔓

doi:10.14148/j.issn.1672-0482.2023.0146

HS-SPME-GC-MS结合化学计量法分析刺五加不同部位的挥发性成分

doi: 10.14148/j.issn.1672-0482.2023.0146

湖北中医药大学湖北省药用植物研发中心, 湖北武汉 430065

基金项目:

湖北省技术创新专项重大项目 2016ACA146

详细信息

作者简介:
何嘉伟, 男, 硕士研究生, E-mail: jw03061713@163.com

通讯作者:
江汉美, 女, 教授, 主要从事中药物质基础及其作用机制研究, E-mail: 13807186811@163.com

中图分类号: R284
计量
- 文章访问数: 169
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- PDF下载量: 15
- 被引次数: 0
出版历程
- 收稿日期: 2022-05-18
- 网络出版日期: 2023-02-17
- 发布日期: 2023-02-10

Analysis of the Volatile Components of Different Parts of Acanthopanax Senticosus by HS-SPME-GC-MS Combined with Chemometric Methods

Medicinal Plant Research and Development Center of Hubei Province, Hubei University of Traditional Chinese Medicine, Wuhan 430065, China

摘要

摘要: 目的对刺五加不同部位的挥发性成分进行比较分析, 为其后续的临床应用及开发提供科学参考。方法采用顶空固相微萃取-气相色谱-质谱联用法(HS-SPME-GC-MS), 提取分析刺五加各部位样品的挥发性成分, 用峰面积归一化法测定和比对各成分的相对含量, 并结合主成分分析(PCA)和聚类分析(HCA)方法分析其成分的差异。结果从刺五加各部位样品中共鉴定出167种挥发性成分, 根、皮、茎、叶、果分别鉴定出71、61、59、48、54种挥发性成分, 主要以烯类和醇类化合物为主。5个部位有10个共有成分, 包括萜品油烯、β-石竹烯、α-律草烯等。HCA分析结果将刺五加根、果聚为一类, 皮、叶聚为一类, 茎单独一类; PCA综合得分依次为根、茎、果、叶、皮。结论刺五加各部位挥发性成分的种类和含量均有差异, 为刺五加非常规药用部位的药食两用深入开发利用提供科学依据。
- 刺五加 /
- 不同部位 /
- 顶空固相微萃取 /
- 气质联用技术 /
- 挥发性成分 /
- 主成分分析
Abstract: OBJECTIVE To conduct a comparative analysis of the volatile components of different parts of Acanthopanax senticosus, and provide scientific reference for their subsequent clinical application and development. METHODS Headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to extract and analyze the volatile components of the samples of various parts of Acanthopanax senticosus. The relative contents of the components were determined and compared by the peak area normalization method, and the differences of the components were analyzed with principal component analysis (PCA) and hierarchical cluster analysis (HCA). RESULTS A total of 167 volatile components were identified from samples of various parts of Acanthopanax senticosus, with 71, 61, 59, 48 and 54 volatile components identified in the root, velamen, stem, leaf and fruit, respectively, mainly as alkenes and alcohols. 10 common components were identified in five parts, including terpinolene, β-staphylene and α-rutinolene, etc. The results of HCA analysis clustered Acanthopanax senticosus root and fruit into one group, velamen and leaf into one group, and stem into a separate group. The PCA scores in the order were: root, stem, fruit, leaf and bark. CONCLUSION The types and contents of volatile components in the various parts of Acanthopanax senticosus are different, providing a scientific basis for the further development and utilization of the unconventional medicinal parts of Acanthopanax senticosus for medicinal and food purposes.
- Acanthopanax senticosus /
- different parts /
- headspace solid-phase microextraction /
- gas chromatography-mass spectrometry /
- volatile components /
- principal component analysis

HTML全文

图 1 各因素对刺五加挥发性成分萃取效果的影响

Figure 1. Effect of various factors on the extraction effect of volatile components of Acanthopanax senticosus

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图 2 刺五加不同部位挥发性成分总离子流图

注: A.根; B.皮; C.茎; D.叶; E.果

Figure 2. Total ion flow diagram of volatile components in different parts of Acanthopanax senticosu

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图 3 刺五加不同部位挥发性成分种类差异图

注:AS-R. 根;AS-V. 皮;AS-S. 茎;AS-L. 叶;AS-F. 果

Figure 3. Differences in the types of volatile components in different parts of Acanthopanax senticosus

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图 4 刺五加不同部位挥发性成分Venn图

注:AS-R. 根;AS-V. 皮;AS-S. 茎;AS-L. 叶;AS-F. 果

Figure 4. Venn map of volatile components in different parts of Acanthopanax senticosus

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图 5 刺五加不同部位挥发性成分聚类热图

注:AS-R. 根;AS-V. 皮;AS-S. 茎;AS-L. 叶;AS-F. 果

Figure 5. Cluster heat map of volatile components in different parts of Acanthopanax senticosus

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图 6 刺五加不同部位挥发性成分PCA散点图

注: AS-R. 根;AS-V. 皮;AS-S. 茎;AS-L. 叶;AS-F. 果

Figure 6. PCA scatter plot of volatile components in different parts of Acanthopanax senticosus

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表 1 刺五加不同部位的挥发性成分及相对含量

Table 1. Volatile components and relative contents in different parts of Acanthopanax senticosus

序号	中文名	英文名	分子式	相对含量/%
序号	中文名	英文名	分子式	根	皮	茎	叶	果
1	正十一烷	Undecane	C₁₁H₂₄	0.94	-	-	-	-
2	正十二烷	Dodecane	C₁₂H₂₆	6.01	-	-	-	-
3	6, 7-二羟基香豆素	Esculetin	C₉H₆O₄	-	0.18	-	-	-
4	萜品油烯	Terpinolene	C₁₀H₁₆	1.62	1.10	0.62	2.50	8.13
5	2, 6, 10-三甲基十二烷	Farnesane	C₁₅H₃₂	0.72	-	-	-	-
6	3-甲基十一烷	3-Methylundecane	C₁₂H₂₆	0.62	-	-	-	-
7	反, 反-2, 4-庚二烯醛	2, 4-Heptadienal	C₇H₁₀O	-	-	-	0.24	-
8	环五聚二甲基硅氧烷	Decamethylcyclopentasiloxane	C₁₀H₃₀O₅Si₅	-	-	-	-	0.11
9	1-乙基环己醇	1-Ethylcyclohexan-1-ol	C₈H₁₆O	0.55	-	-	-	-
10	(-)-顺式-桃金娘烷醇	(-)-cis-Myrtanol	C₁₀H₁₈O	-	0.96	-	-	-
11	5-乙基癸烷	5-Ethyldecane	C₁₂H₂₆	0.64	-	-	-	-
12	3-辛烯-2-酮	3-Octen-2-one	C₈H₁₄O	-	-	0.11	-	-
13	4-异丙烯基甲苯	4, α-Dimethylstyrene	C₁₀H₁₂	-	-	0.33	-	0.57
14	4-乙基癸烷	4-Ethyldecane	C₁₂H₂₆	0.37	-	-	-	-
15	5-甲基-十一烷	5-Methylundecane	C₁₂H₂₆	0.47	-	-	-	-
16	2-甲基-1-苯基丙烯	2-Methyl-1-phenylpropene	C₁₀H₁₂	-	0.23	-	-	-
17	2-己基-1-辛醇	2-Hexyloctan-1-ol	C₁₄H₃₀O	0.13	-	-	-	-
18	4-侧柏醇	Sabinene hydrate	C₁₀H₁₈O	-	-	0.42	0.26	1.5
19	乙酸	Hexanoic acid	C₆H₁₂O₂	3.34	-	3.42	0.23	1.47
20	油酸	Oleic acid	C₁₈H₃₄O₂	0.38	-	-	-	-
21	异蒲勒醇	Isopulegol	C₁₀H₁₈O	-	0.17	-	-	-
22	2-壬酮	2-Nonanon	C₉H₁₈O	-	-	-	-	0.1
23	壬醛	Nonanal	C₉H₁₈O	2.26	-	0.42	-	-
24	芳樟醇	Linalool	C₁₀H₁₈O	-	1.43	-	1.19	1.22
25	3-亚甲基十一烷	3-Methylideneundecane	C₁₂H₂₄	0.22	-	-	-	-
27	硬脂酸苯酯	Phenyl stearate	C₂₄H₄₀O₂	-	0.16	-	-	-
28	苯甲醇	Benzyl alcohol	C₇H₈O	-	-	-	0.15	0.17
29	1-癸烯	1-Decene	C₁₀H₂₀	-	0.28	-	-	-
30	(-)-α-侧柏酮	(-)-α-Thujone	C₁₀H₁₆O	-	-	0.28	-	-
32	植物醇	Phytol	C₂₀H₄₀O	0.11	-	-	-	-
33	3, 5-辛二烯-2-酮	3, 5-Octadien-2-one	C₈H₁₂O	-	-	-	0.16	-
34	白菖醇	6-epi-Shyobunol	C₁₅H₂₆O	0.21	-	-	-	-
35	6-甲基-5-庚烯-2-醇	Sulcatol	C₈H₁₆O	-	-	-	0.5	-
36	龙脑烯醛	(R)-α-Campholenaldehyde	C₁₀H₁₆O	-	0.29	0.59	-	0.19
37	(-)-补身醇	Drimenol	C₁₅H₂₆O	0.12	-	-	-	-
39	(-)-反式一松香芹醇	(-)-trans-Pinocarveol	C₁₀H₁₆O	-	0.38	0.61	-	0.23
40	(1R)-(+)-诺蒎酮	(1R)-(+)-Nopinone	C₉H₁₄O	-	-	-	0.26	-
41	4-(1-甲基乙基)环己醇	4-Isopropylcyclohexanol	C₉H₁₈O	-	-	-	-	0.32
42	樟脑	Camphor	C₁₀H₁₆O	1.36	-	-	-	0.25
43	异薄荷酮	Isomenthone	C₁₀H₁₈O	-	-	-	0.82	-
44	异橙花醛	Isoneral	C₁₀H₁₆O	-	0.28	-	-	-
45	反式马鞭草酚	trans-Verbenol	C₁₀H₁₆O	-	-	0.41	-	-
46	α-环氧蒎烷	α-Pinene oxide	C₁₀H₁₆O	-	0.62	-	-	-
47	萜品烯	4-Terpineol	C₁₀H₁₈O	2.38	1.28	0.72	1.24	5.4
48	反式-2-壬烯醛	trans-2-Nonenal	C₉H₁₆O	0.75	-	-	-	-
49	匹诺酮	Pinocarvone	C₁₀H₁₄O	-	-	0.27	-	-
50	螺内酯	Spironolactone	C₂₄H₃₂O₄S	-	0.17	-	-	-
51	苯乙醇	2-Phenylethanol	C₈H₁₀O	-	-	-	-	0.69
52	薄荷脑	2-Isopropyl-5-methylcyclohexanol	C₁₀H₂₀O	1.36	-	-	12.75	-
53	L-薄荷醇	L-Menthol	C₁₀H₂₀O	-	0.61	0.38	-	-
54	冰片	Borneol	C₁₀H₁₈O	0.93	0.28	0.1	-	0.24
55	4-烯丙基苯甲醚	Estragole	C₁₀H₁₂O	6.86	0.62	1.88	0.47	4.04
57	十三烷	Tridecane	C₁₃H₂₈	2.97	-	-	-	-
58	α-松油醇	α-Terpineol	C₁₀H₁₈O	-	0.85	-	0.15	0.99
59	5-异丙基双环[3.1.0]己烷-2-酮	1-Propan-2-ylbicyclo[3.1.0]hexan-4-one	C₉H₁₄O	-	-	0.77	-	-
60	2-异丙基-5-甲基茴香醚	Thymyl methyl ether	C₁₁H₁₆O	0.53	-	-	-	-
61	桃金娘烯醛	Myrtenal	C₁₀H₁₄O	-	0.19	0.42	-	-
62	1, 3, 3-三甲基-2-乙烯基环己烯	1, 3, 3-Trimethyl-2-vinyl-1-cyclohexene	C₁₁H₁₈	-	-	-	-	0.67
63	1-溴十八烷	1-Bromooctadecane	C₁₈H₃₇Br	0.32	-	-	-	-
64	三甲基苯甲醇	2-(4-Methylphenyl)propan-2-ol	C₁₀H₁₄O	-	0.2	0.57	-	0.48
65	乙酸芳樟酯	Linalyl acetate	C₁₂H₂₀O₂	-	-	-	0.46	-
66	β-环柠檬醛	β-Cyclocitral	C₁₀H₁₆O	-	-	-	0.19	-
67	4-异丙基环己-2-烯-1-酮	4-(1-Methylethyl)-2-cyclohexen-1-one	C₉H₁₄O	-	-	-	-	0.12
68	7-甲基十七烷	7-Methylheptadecane	C₁₈H₃₈	0.15	-	-	-	-
69	邻硝基苯十二烷醚	1-Dodecoxy-2-nitrobenzene	C₁₈H₂₉NO₃	0.24	-	-	-	-
70	(1R, 5R)-rel-香芹醇	cis-Carveol	C₁₀H₁₆O	-	0.25	0.38	-	-
71	优葛缕酮	Eucarvone	C₁₀H₁₄O	-	-	-	-	0.42
72	薄荷醛	3-p-Menthen-7-al	C₁₀H₁₆O	0.73	-	-	-	-
73	α-菖蒲醇	α-acorenol	C₁₅H₂₆O	0.25	0.16	-	-	0.12
74	马鞭草烯醇	(S)-(-)-Verbenone	C₁₀H₁₄O	-	-	0.29	-	-
75	乙酸龙脑酯	Bornyl acetate	C₁₂H₂₀O₂	1.78	-	-	-	-
76	δ-榄香烯	δ-Elemene	C₁₅H₂₄	-	3.58	0.32	1.99	2.58
77	4-异丙基苯甲醛	Cuminaldehyde	C₁₀H₁₂O	1.24	-	-	-	-
78	(-)-α-荜澄茄油烯	(-)-α-Cubebene	C₁₅H₂₄	-	-	3.16	0.45	-
79	正戊基2-呋喃酮	2-Hexanoylfuran	C₁₀H₁₄O₂	-	0.57	-	-	-
80	α-长叶蒎烯	α-Longipinene	C₁₅H₂₄	-	5.12	0.7	-	-
81	3, 5-二甲氧基甲苯	3, 5-Dimethoxytoluene	C₉H₁₂O₂	-	-	-	-	1.88
82	黄樟素	Safrole	C₁₀H₁₀O₂	-	-	-	-	1.84
83	异黄樟素	Isosafrole	C₁₀H₁₀O₂	-	0.24	-	-	-
84	胡椒酮	Piperitone	C₁₀H₁₆O	-	-	-	1.63	-
85	茴香脑	Anethole	C₁₀H₁₂O	21.08	-	-	-	-
86	雪松烯	Cedrene	C₁₅H₂₄	0.4	1.01	-	-	0.44
87	正十四烷	Tetradecane	C₁₄H₃₀	-	-	1.08	-	-
88	(-)-α-蒎烯	α-Copaene	C₁₅H₂₄	1.66	0.67	2.23	0.85	1.15
89	(+)-1, 7-二表-α-雪松烯	Di-epi-α-cedrene	C₁₅H₂₄	-	0.2	-	-	-
90	α-愈创木烯	α-Guaiene	C₁₅H₂₄	-	-	0.5	-	-
91	(+)-香橙烯	Aromandendrene	C₁₅H₂₄	-	-	-	-	2.01
92	表蓝桉醇	Epiglobulol	C₁₅H₂₆O	-	-	0.27	-	-
93	2, 5-十八碳二炔酸甲酯	Methyl octadeca-2, 5-diynoate	C₁₉H₃₀O₂	-	0.2	-	-	-
94	巴伦西亚橘烯	(+)-Valencene	C₁₅H₂₄	2.72	-	-	-	-
95	β-榄香烯	β-Elemene	C₁₅H₂₄	-	3.28	2.7	3.6	1.18
96	肉桂醛	Cinnamaldehyde	C₉H₈O	-	1.96	-	-	2.2
97	(-)-α-雪松烯	(-)-α-Cedrene	C₁₅H₂₄	2.3	-	-	-	-
98	3-吡啶丙烯酸乙酯	Ethyl 3-pyridineacrylate	C₁₀H₁₁NO₂	-	-	0.54	-	-
99	毕澄茄烯	β-Cubebene	C₁₅H₂₄	-	-	0.22	0.59	-
100	β-石竹烯	Caryophyllene	C₁₅H₂₄	4.23	5.78	5.22	7.52	7
101	马兜铃-9-烯	(-)-Aristolene	C₁₅H₂₄	-	-	-	0.62	0.4
102	香树烯	Aromandendrene	C₁₅H₂₄	0.85	1.77	0.61	0.13	-
103	E-β-金合欢烯	cis-β-Farnesene	C₁₅H₂₄	0.79	2.8	5.14	4.78	13.74
104	正十五烷	Pentadecane	C₁₅H₃₂	0.52	-	1.5	-	-
105	去氢香树烯	Dehydro-Aromadendrene	C₁₅H₂₂	-	0.34	-	-	-
106	α-律草烯	Humulene	C₁₅H₂₄	1.15	3.39	2.76	5.52	3.25
107	γ-依兰油烯	γ-Muurolene	C₁₅H₂₄	-	2.1	2.91	2.92	2.28
108	甲基丁香酚	O-methyleugenol	C₁₁H₁₄O₂	0.19	0.41	-	-	-
109	反式-α-香柑油烯	trans-α-Bergamotene	C₁₅H₂₄	-	-	-	-	0.83
110	α-姜黄烯	α-Curcumene	C₁₅H₂₂	-	3.6	14.75	0.7	0.65
111	β-芹子烯	β-Selinene	C₁₅H₂₄	6.12	2.61	-	2.85	4.95
112	α-芹子烯	α-Selinene	C₁₅H₂₄	1.88	-	-	-	-
113	β-红没药烯	β-Bisabolene	C₁₅H₂₄	-	12.74	4.38	9.51	10.71
114	香叶基丙酮	Geranylacetone	C₁₃H₂₂O	0.17	-	-	0.13	-
115	β-杜松烯	β-Cadinene	C₁₅H₂₄	2.13	2.56	9.57	2.25	1.64
116	倍半萜桧烯	Sesquisabinene	C₁₅H₂₄	-	1.22	2.26	1.97	-
117	荜澄茄油烯	Cubenene	C₁₅H₂₄	0.29	-	1.64	-	-
118	α-依兰油烯	α-Muurolene	C₁₅H₂₄	0.42	-	-	0.15	0.19
119	佛术烯	(+)-Valencene	C₁₅H₂₄	-	1.02	-	-	-
120	荜澄茄醇	Cubebol	C₁₅H₂₆O	0.13	-	1.96	-	-
121	正十六烷	Hexadecane	C₁₆H₃₄	0.13	-	-	-	-
122	氯代十八烷	1-Chlorooctadecane	C₁₈H₃₇Cl	-	-	0.43	-	-
123	异戊酸香叶酯	Geranyl isovalerate	C₁₅H₂₆O₂	-	0.39	-	-	-
124	香草醛乳糖苷	Vanillin lactoside	C₂₀H₂₈O₁₃	0.18	-	-	-	-
125	β-紫罗兰酮	β-Ionone	C₁₃H₂₀O	-	-	-	1.05	-
126	β-愈创木烯	β-Guaiene	C₁₅H₂₄	-	-	-	-	0.14
127	9-甲氧基菖蒲烯	9-Methoxycalamenene	C₁₆H₂₄O	-	0.21	0.33	-	0.16
128	13, 14-二氢-15(R)-前列腺素E1	13, 14-Dihydro-15(R)-Prostaglandin E1	C₂₀H₃₆O₅	0.23	-	-	-	-
129	瑞香[草]氫醌	p-Cymene-2, 5-diol	C₁₀H₁₄O₂	0.12	-	-	-	-
130	正三十七醇	1-Heptatriacotanol	C₃₇H₇₆O	-	-	0.16	-	0.22
131	异丁香酚甲醚	Isomethyleugenol	C₁₁H₁₄O₂	-	0.33	-	-	-
132	橙花叔醇	Nerolidol	C₁₅H₂₆O	-	-	1.59	0.14	-
133	肉豆蔻醚	Myristicin	C₁₁H₁₂O₃	-	0.83	-	-	0.12
134	二苯并呋喃	Dibenzofuran	C₁₂H₈O	0.15	-	-	-	-
135	4(15), 5, 10(14)-大根香叶三烯-1-醇	4(15), 5, 10(14)-Germacratrien-1-ol	C₁₅H₂₄O	-	0.8	1.55	1.3	0.37
136	2-反式十八碳烯-1-醇	E-2-Octadecadecen-1-ol	C₁₈H₃₆O	0.14	-	-	-	-
137	正十七烷	Heptadecane	C₁₇H₃₆	0.39	-	1.94	0.41	-
138	美他帕明	Metapramine	C₁₆H₁₈N₂	-	0.89	-	-	-
139	桉油烯醇	10'-Apocarotenal	C₁₅H₂₄O	0.13	6.03	3.49	5.81	0.62
140	异匙叶桉油烯醇	Isospathulenol	C₁₅H₂₄O	-	-	0.35	5.97	3.66
141	环氧马兜铃烯	Aristolene epoxide	C₁₅H₂₄O	-	0.4	1.33	0.82	0.46
142	环氧化蛇麻烯Ⅱ	(1R, 3Z, 7Z, 11S)-1, 5, 5, 8-Tetramethyl-12-oxabicyclo[9.1.0]dodeca-3, 7-diene	C₁₅H₂₄O	-	0.29	0.39	0.47	0.12
143	(-)-蓝桉醇	(-)-Globulol	C₁₅H₂₆O	-	-	-	-	0.17
144	异水菖蒲酮	Isoshyobunone	C₁₅H₂₄O	-	0.33	0.35	-	-
145	异香树烯环氧化物	Isoaromadendrene epoxide	C₁₅H₂₄O	-	-	0.75	0.24	-
146	T-杜松醇	T-cadino	C₁₅H₂₆O	-	-	0.69	-	-
147	红没药醇氧化物	Bisabolol oxide B	C₁₅H₂₆O₂	-	7.62	-	-	-
148	邻苯二甲酸二乙酯	Diethyl phthalate	C₁₂H₁₄O₄	0.3	-	-	-	-
149	(-)-愈创醇	Guaiol	C₁₅H₂₆O	-	1.56	-	-	-
150	百秋李醇	Patchouli alcohol	C₁₅H₂₆O	0.1	-	-	-	-
151	氧化喇叭烯	Ledene oxide-(Ⅰ)	C₁₅H₂₄O	-	-	0.35	-	-
152	2, 6, 10-三甲基十四烷	2, 6, 10-Trimethyltetradecane	C₁₇H₃₆	0.1	-	-	-	-
153	红没药醇	α-Bisabolol	C₁₅H₂₆O	-	8.24	0.3	8.15	2.82
154	石竹素	(-)-Caryophyllene oxide	C₁₅H₂₄O	0.25	0.16	1.88	1.33	0.53
155	韦得醇	Methyl 4-heptylbenzoate	C₁₅H₂₂O₂	-	-	-	-	0.27
156	金合欢醇	Farnesol	C₁₅H₂₆O	-	0.13	-	-	-
157	2, 6-二氨基庚二酸	2, 6-Diaminopimelic acid	C₇H₁₄N₂O₄	0.29	-	-	-	-
158	Z-亚丁基苯酞	Z-Butylidenephthalide	C₁₂H₁₄O₂	0.83	-	-	-	-
159	3-丁基-1(3H)-异苯并呋喃酮	3-Butylisobenzofuran-1(3H)-one	C₁₂H₁₄O₂	1.23	-	-	-	-
160	西松烯	Cembrene	C₂₀H₃₂	-	0.24	0.38	0.4	-
161	α-香附酮	α-Cyperone	C₁₅H₂₂O₂	0.17	-	-	-	-
162	植酮	Phytone	C₁₈H₃₆O	-	-	-	0.17	-
163	E-藁本内酯	E-ligustilide	C₁₂H₁₄O₂	2.39	-	-	-	-
164	新蛇床内酯	Neocnidilide	C₁₂H₁₈O₂	0.66	-	-	-	-
165	川芎内酯	Senkyunolide	C₁₂H₁₆O₂	1	-	-	-	-
166	海松-8(14), 15-二烯	Pimara-8(14), 15-diene	C₂₀H₃₂	0.69	-	-	-	-
167	棕榈酸	Palmitic acid	C₁₆H₃₂O₂	0.27	-	-	-	-
注: -表示目前分析方法下无法检出。

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表 2 刺五加不同部位挥发性成分的种类及相对含量(%)

Table 2. Types and relative contents of volatile components in different parts of Acanthopanax senticosus(%)

类别	根		皮		茎		叶		果
类别	数量	相对含量	数量	相对含量	数量	相对含量	数量	相对含量	数量	相对含量
烷类	15	14.57	1	0.62	4	4.95	1	0.41	1	0.11
烯类	19	29.63	26	57.82	24	63.19	22	51.83	23	68.08
醛类	4	4.98	4	2.72	3	1.43	2	0.43	2	2.39
醇类	10	1.87	14	21.77	15	13.01	10	23.62	16	13.85
酮类	3	1.57	2	0.90	5	1.78	7	4.22	3	0.64
酯类	5	6.13	7	0.92	1	0.54	1	0.46	0	0
酚类	1	0.19	1	0.41	1	0.41	0	0	0	0
醚类	3	7.63	3	1.78	1	1.88	1	0.47	2	4.16
芳香族类	4	24.73	3	0.70	2	0.43	1	12.75	5	4.78
氧化物类	1	0.25	2	7.78	2	2.63	2	1.57	1	0.53
有机酸类	4	4.28	0	0	1	3.42	1	0.23	1	1.47
其他类	5	1.51	1	0.89	0	0	0	0	0	0

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表 3 主成分的特征值及其贡献率

Table 3. The eigenvalues of principal components and their contribution rates

主成分	初始特征值
主成分	合计	方差/%	累积/%
1	2.50	50.04	50.04
2	1.00	20.06	70.10
3	0.71	14.25	84.35
4	0.43	8.63	92.98
5	0.35	7.02	100.00

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表 4 刺五加不同部位挥发性成分因子得分、主成分得分和综合得分

Table 4. Factor scores, principal component scores and composite scores of volatile components in different parts of Acanthopanax senticosus

部位	因子得分		主成分得分		综合得分(Y)
部位	F₁	F₂	Y₁	Y₂	综合得分(Y)
根	1.04	0.16	1.64	0.26	1.25
皮	-0.60	-0.91	-0.95	-1.44	-1.09
茎	-0.61	1.02	-0.96	1.62	-0.22
叶	-0.60	-0.43	-0.95	-0.68	-0.87
果	-0.35	-0.61	-0.56	-0.97	-0.67

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