基于UPLC-Q-TOF-MS/MS技术分析前胡化学物质组及体内成分

吉春亮; 苏艳; 张金戈; 盛先森; 张健榕; 单晨啸

doi:10.14148/j.issn.1672-0482.2022.1143

基于UPLC-Q-TOF-MS/MS技术分析前胡化学物质组及体内成分

doi: 10.14148/j.issn.1672-0482.2022.1143

1.
句容市第四人民医院药剂科, 江苏镇江 212008
2.
南京中医药大学药学院, 江苏南京 210023

基金项目:

江苏省中医药管理局科技项目 YB201809

详细信息

作者简介:
吉春亮, 男，主管药师, E-mail: 692993528@qq.com

通讯作者:
单晨啸, 男，实验师, 主要从事高分辨质谱分析方法研究, E-mail: 300618@njucm.edu.cn

中图分类号: R284
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- 被引次数: 0
出版历程
- 收稿日期: 2022-09-29
- 网络出版日期: 2022-12-15
- 发布日期: 2022-12-10

Identification of the Chemical Components of Peucedani Radix and in vivo Chemicals Based on UPLC-Q-TOF-MS/MS

1.
Department of Pharmacy, The Fourth People's Hospital of Jurong City, Zhenjiang 212008, China
2.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

摘要

摘要: 目的通过UPLC-Q-TOF-MS/MS研究前胡的化学物质组, 探究其被吸收入血和肺组织中的原型成分及代谢物。方法采用Thermo Acclaim^TM RSLC 120 C₁₈柱(3.0 mm×100 mm, 2.2 μm), 柱温: 35 ℃, 以0.1%甲酸水-乙腈为流动相进行梯度洗脱, 流速为0.3 mL · min^-1, 进样量为5 μL。采用电喷雾(ESI)离子源, 正离子模式下采集质谱信息。结果根据化合物的精确m/z、二级碎片等质谱信息, 结合标准品的质谱信息和比对文献检索信息, 最终在前胡提取液中共推测出54个化学成分, 包括52个香豆素类化合物、1个萜烯类化合物和1个酚类化合物; 给药后在大鼠血浆中共鉴定出21个原型成分和26个代谢物, 肺组织中共鉴定出17个原型成分和9个代谢物。结论该方法快速、准确、全面, 其分析结果为前胡的质量控制以及进一步的药效物质基础研究奠定了基础。
- 前胡 /
- 成分分析 /
- UPLC-Q-TOF-MS/MS /
- 定性 /
- 香豆素类
Abstract: OBJECTIVE To deduce the main chemical components in Peucedani radix and its absorbed prototype components and metabolites in plasma and lung using a UPLC-Q-TOF-MS/MS method. METHODS Thermo Acclaim^TM RSLC 120 C₁₈ column (3.0 mm×100 mm, the particle size is 2.2 μm) was used by using a gradient elution program with 0.1% formic acid water and acetonitrile as mobile phase. Column temperature was set at 35 ℃, the flow rate was 0.3 mL · min^-1 and the injection volume was 5 μL. ElectroSpray Ionization Source (ESI) was selected to collect parent and product mass spectrometric data in positive ion mode. RESULTS The chemical components in Peucedani radix were analyzed and identified according to the accurate mass, retention time, the fragmentation and other information of the compounds, especially, by comparing the reference substances and literature data. A total of 54 chemical constituents were identified in Peucedani radix, including 52 coumarins, 1 terpene and 1 phenolic compound. And a total of 21 prototype components and 26 metabolites were identified in rat plasma. 17 prototype components and 9 metabolites were identified in lung tissue after oral administration of Peucedani radix extract to rats. CONCLUSION This method is rapid, accurate and comprehensive. The analysis results lay a foundation for the quality control of Peucedani radix and the further study of pharmacological material basis.
- Peucedani radix /
- component analysis /
- UPLC-Q-TOF-MS/MS /
- qualitative /
- coumarins

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图 1 正离子模式下的前胡提取液(A)及混合对照品(B)的总离子流图

Figure 1. The total ion chromatogram of Peucedani radix extract (A) and the reference standards solution (B) in positive ion mode

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图 2 前胡中典型成分的提取离子流图及裂解规律图

Figure 2. MS/MS spectrums and extract ion chromatography of typical components in Peucedani radix extract

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图 3 典型入血原型成分的提取离子流图及裂解规律

Figure 3. MS/MS spectrums and extract ion chromatography of typical prototype components absorbed into the plasma after oral administration of Peucedani radix extract

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图 4 大鼠给药前胡提取物后血浆中代谢成分的裂解规律图

Figure 4. MS/MS spectrums and extract ion chromatography of absorbed typical metabolites in rat plasma

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表 1 前胡体外化学成分鉴定

Table 1. Identification of chemical components in Peucedani radix extract

编号	RT/min	化学式	分子量	离子归属	理论值	实际值	误差ppm	强度	碎片离子	化合物名称	类型
1	2.389	C₂₀H₂₄O₁₂	456.126 8	+H	457.134 1	457.134 4	0.8	39 575	163.039 6, 119.049 9, 107.051 0	洋芫荽茵芋苷	1
2	2.428	C₉H₆O₃	162.031 7	+H	163.039 0	163.039 4	2.8	108 356	135.044 4, 119.049 9, 107.051 1, 91.056 4, 77.042 4	伞形花内酯	1
3	2.432	C₁₅H₁₆O₈	324.084 5	+H	325.091 8	325.091 8	0	162 172	163.039 8, 119.051 5, 91.057 4, 77.042 4	茵芋苷	1
4	2.874	C₁₆H₁₈O₉	354.095 1	+H	355.102 4	355.103 1	2	53 214	193.050 7, 178.027 4, 163.039 7, 133.028 9	东莨菪苷	1
5	2.949	C₂₁H₂₆O₁₃	486.137 3	+H	487.144 6	487.144 9	0.6	27 648	193.050 3, 133.028 9	Hymexelsin	1
6	6.897	C₂₀H₂₄O₁₀	424.137 0	+Na	447.126 2	447.126 5	0.8	131 885	285.073 7	Isorutarin	1
7	7.340	C₁₀H₈O₄	192.042 3	+H	193.049 5	193.050 2	3.3	79 628	178.026 7, 150.032 5, 137.061 0, 133.029 9, 122.037 3, 94.044 0	东莨菪素	1
8	8.017	C₁₀H₈O₃	176.047 3	+H	177.054 6	177.055 1	2.7	10 878	145.026 8, 117.035 4, 89.040 8	4-甲氧基香豆素	1
9	8.266	C₁₁H₁₀O₅	222.052 8	+H	223.060 1	223.060 9	3.4	186 154	207.029 7, 190.026 3, 162.031 6	异嗪皮啶	1
10	8.852	C₂₀H₂₄O₁₀	424.137 0	+Na	447.126 2	447.126 2	0	26 934	285.072 9	Rutarin	1
11	9.208	C₁₄H₁₆O₅	264.099 8	+H	265.107 1	265.107 5	1.8	69 573	247.096 0, 229.083 7, 175.040 2, 147.045 4	(+)-白花前胡醇	1
12	9.211	C₂₀H₂₆O₁₀	426.152 6	+H	427.159 9	427.158 8	1.2	129 301	247.097 5, 229.086 9, 175.040 3	Praeroside Ⅵ	1
13	10.225	C₁₄H₁₆O₅	264.099 8	+H	265.107 1	265.107 2	0.6	15 587	229.087 9, 175.038 9, 147.043 6, 115.056 8	(-)-白花前胡醇	1
14	11.019	C₂₀H₂₄O₉	408.142 0	+H	409.149 3	409.150 1	2	432 012	247.096 6, 229.086 2, 187.039 2, 175.039 4	紫花前胡苷	1
15	11.130	C₁₄H₁₄O₅	262.084 1	+Na	285.073 3	285.073 0	-1.3	3 106	243.051 5, 215.066 9, 159.081 4, 153.072 9, 128.068 2	前胡香豆素G	1
16	12.382	C₂₀H₂₄O₁₀	424.137 0	+Na	447.126 2	447.126 4	0.6	19 337	285.072 2	Apterin	1
17	13.452	C₁₄H₁₄O₅	262.084 1	+Na	285.073 3	285.073 9	1.8	21 828	225.170 4, 165.076 6, 153.074 1	芸香霉素	1
18	14.560	C₂₀H₂₄O₉	408.142 0	+Na	431.131 3	431.130 8	-1.0	5 866	369.099 3, 175.044 2	异紫花前胡苷	1
19	15.022	C₁₄H₁₄O₄	246.089 2	+Na	269.078 4	269.078 1	-1.1	7 447	225.102 4, 131.084 2, 105.070 8	紫花前胡苷元	1
20	17.961	C₁₇H₁₈O₇	334.105 3	+H	335.112 5	335.113 4	2.6	15 429	233.045 5, 218.021 8, 173.024 4	白当归素	1
21	18.430	C₁₁H₆O₃	186.031 7	+H	187.039 0	187.039 7	3.8	99 740	143.049 7, 131.050 8, 115.056 1, 103.056 5, 77.043 0	补骨脂素	1
22	18.875	C₁₀H₈O₃	176.047 3	+H	177.054 6	177.054 9	1.3	48 373	145.029 0, 117.035 1, 89.041 8	5-甲氧基香豆素	1
23	20.098	C₁₂H₈O₄	216.042 3	+H	217.049 5	217.050 4	4.0	94 076	202.027 3, 174.032 0, 146.037 6, 118.043 1	佛手柑内酯	1
24	20.340	C₁₂H₈O₄	216.042 3	+H	217.049 5	217.050 5	4.5	1 009 484	202.026 0, 174.031 4, 118.043 1	6-甲氧基当归素	1
25	21.653	C₁₆H₁₈O₄	274.120 5	+Na	297.109 7	297.112 8	10.0	24 854	261.091 4, 231.080 1, 157.066 2, 107.050 9	九里香内酯	1
26	23.742	C₁₂H₈O₄	216.042 3	+H	217.049 5	217.050 5	4.2	431 058	202.026 5, 174.031 7, 146.037 4, 118.043 4	花椒毒素	1
27	23.911	C₁₃H₁₀O₅	246.052 8	+Na	269.042 0	269.041 7	-1.4	6 977	227.073 4, 203.039 2, 173.022 2	异茴芹内酯	1
28	25.025	C₁₅H₁₆O₃	244.109 9	+H	245.117 2	245.117 3	0.3	38 335	227.071 4, 203.071 9, 175.040 3, 147.043 5, 131.057 4	蛇床子素	1
29	28.141	C₁₆H₁₄O₅	286.084 1	+H	287.091 4	287.091 3	-0.3	86 059	245.079 5, 227.072 4, 175.034 7, 147.044 7	异氧化前胡素	1
30	28.143	C₂₁H₂₂O₇	386.136 6	+H	387.143 8	387.143 7	-0.3	63 199	327.121 3, 245.081 5, 227.071 3, 175.039 7, 147.044 6	蛇床明素	1
31	28.435	C₁₈H₁₆O₆	328.094 7	+H	329.102 0	329.103 6	5.1	16 982	311.223 0, 293.214 2, 245.080 1, 227.072 0, 175.040 2	Sclerodin	1
32	29.532	C₁₈H₁₈O₇	346.105 3	+Na	369.094 5	369.094 5	0	147 528	309.074 3, 287.092 5, 245.081 6, 227.070 1, 175.039 4, 147.044 0, 128.063 1	前胡香豆素D	1
33	30.380	C₁₁H₆O₃	186.031 7	+H	187.039 0	187.039 4	2.1	4 156	131.050 6, 115.055 2, 103.057 1, 77.044 7	异补骨脂素	1
34	31.059	C₁₉H₂₀O₆	344.126 0	+Na	367.115 2	367.115 4	0.4	121 210	267.063 7, 203.071 1, 175.038 8, 147.045 7	前胡香豆素A	1
35	31.989	C₂₇H₃₈O₁₅	602.221 1	+Na	625.210 3	625.207 4	-4.6	21 721	487.131 9, 351.087 8, 121.066 9	为云前胡苷	1
36	32.218	C₁₆H₂₀O₆	308.126 0	+Na	331.115 2	331.118 6	10.0	48 115	261.077 4, 243.066 6, 215.070 2, 203.035 8, 189.019 2, 159.045 1	九里香素	1
37	32.934	C₁₇H₂₀O₁₀	384.105 7	+H	385.112 9	385.113 1	0.3	22 636	283.021 2, 227.065 3, 141.020 6	刺五加苷B1	1
38	33.502	C₁₆H₁₄O₄	270.089 2	+H	271.096 5	271.097 4	3.5	263 447	203.034 3, 175.039 4, 147.045 2, 131.050 4, 91.057 5	欧前胡素	1
39	34.013	C₁₉H₁₈O₆	342.110 3	+Na	365.099 6	365.100 8	3.3	31 094	265.048 0, 251.033 3	前胡香豆素E	1
40	35.843	C₁₉H₂₀O₅	328.131 1	+Na	351.120 3	351.120 4	0.4	57 374	333.178 1, 213.055 4, 157.067 9, 128.066 9	紫花前胡素	1
41	36.013	C₁₆H₁₄O₄	270.089 2	+H	271.096 5	271.097 1	2.3	104 003	203.035 0, 159.045 2, 147.045 3, 131.050 6, 91.057 9	异欧前胡素	1
42	36.122	C₁₀H₈O₄	192.042 3	+H	193.049 5	193.050 0	2.2	150 047	193.050 4, 178.026 4, 149.061 1, 137.061 5, 121.066 5, 109.067 1, 94.043 8, 77.042 9	异莨菪亭	1
43	36.225	C₂₁H₂₂O₇	386.136 6	+Na	409.125 8	409.126 3	1.3	60 740	349.105 8, 327.123 2, 245.082 6, 227.071 3, 175.039 1, 128.062 8, 83.053 5	白花前胡甲素^*	1
44	36.453	C₁₄H₁₀O₃	226.063 0	+H	227.070 3	227.070 8	2.5	675 663	199.075 0, 128.063 6	Arnocoumarin	1
45	36.456	C₁₆H₁₄O₅	286.084 1	+H	287.091 4	287.092 1	2.5	2 557 621	245.080 5, 227.070 0, 175.038 9, 147.044 8, 128.062 9	氧化前胡素	1
46	36.483	C₂₁H₂₂O₇	386.136 6	+Na	409.125 8	409.126 0	0.4	217 601	349.106 3, 309.074 4, 245.081 6, 227.071 7, 175.039 3, 128.062 9, 83.053 2	白花前胡香豆精Ⅱ	1
47	37.667	C₂₁H₂₄O₇	388.152 2	+Na	411.141 4	411.141 2	-0.6	193 743	351.121 7, 329.138 3, 245.081 5, 227.070 8, 175.039 5, 128.062 3	白花前胡香豆精Ⅰ	1
48	37.685	C₁₄H₂₂O	206.167 1	+H	207.174 3	207.175 7	6.7	7 362	189.165 5, 151.112 7, 123.082 3	3, 5-二叔丁基苯酚	2
49	38.946	C₂₀H₂₄O₄	328.167 5	+H	329.174 7	329.175 1	1	133 723	205.050 3, 190.026 8, 162.032 3	Sesibiricin	1
50	42.284	C₂₄H₂₆O₇	426.167 9	+Na	449.157 1	449.156 8	-0.7	169 613	349.105 4, 327.120 4, 227.070 7, 83.054 2	白花前胡乙素^*	1
51	42.556	C₂₄H₂₈O₇	428.183 5	+Na	451.172 7	451.171 8	-2.1	44 457	351.121 1, 245.082 4, 227.072 4, 175.040 2, 83.053 2	白花前胡丙素	1
52	43.110	C₂₄H₂₈O₇	428.183 5	+Na	451.172 7	451.170 7	-4.5	170 362	349.1142, 245.082 1, 227.068 5, 215.071 9, 175.041 1, 83.051 2	白花前胡素E^*	1
53	45.072	C₁₅H₂₄O	220.182 7	+H	221.190 0	221.190 0	0	103 117	201.181 1, 135.118 0, 119.086 9, 81.073 4	氧化石竹烯	3
54	49.454	C₂₄H₂₈O₇	428.183 5	+Na	451.172 7	451.171 3	-3.0	16 294	351.113 5, 245.081 2, 227.071 7, 175.038 0, 83.052 9	前胡香豆素H	1
注: ^*表示经过标准品对照。1.香豆素类；2.酚类；3.萜烯类。

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表 2 大鼠灌胃前胡提取物后的体内原型及代谢物信息

Table 2. Identification of in vivo prototypes and metabolites after oral administration of Peucedani radix extract

编号	RT/min	化学式	分子量	离子归属	理论值	实际值	误差ppm	碎片离子	名称	X-116	X-224	X-437	F-116	F-220	F-418
M1	2.210	C₁₁H₇O₃	187.039 5	+H	188.046 8	188.070 4	5.3	143.073 0, 118.065 9, 91.056 9	欧前胡素-C₅H₈O		+	+	+	+
M2	3.460	C₂₂H₂₂O₁₁	462.116 2	+H	463.123 5	463.122 4	-2.5	287.088 1, 269.079 4	氧化前胡素+Glu			+
M3	7.040	C₂₀H₂₂O₁₁	438.116 2	+H	439.123 5	439.123 7	0.5	263.091 9, 245.082 0, 191.034 0, 163.037 9	芸香霉素+Glu			+	+	+	+
P8	8.017	C₁₀H₈O₃	176.047 3	+H	177.054 6	177.055 1	2.7	145.033 0, 122.947 8, 117.033 7	4-甲氧基香豆素	+	+	+		+
P11	9.208	C₁₄H₁₆O₅	264.099 8	+H	265.107 1	265.107 5	1.8	247.084 1, 205.077 4, 175.046 5, 163.078 4, 147.044 9	(+)-白花前胡醇		+
P13	10.225	C₁₄H₁₆O₅	264.099 8	+H	265.107 1	265.107 2	0.6	175.039 8, 147.035 6, 115.068 5	(-)-白花前胡醇		+	+
P15	11.130	C₁₄H₁₄O₅	262.084 1	+Na	285.073 3	285.073 0	-1.3	243.015 5, 207.029 0, 127.978 8	前胡香豆素G	+	+	+	+	+	+
M4	12.003	C₁₃H₈O₅	244.037 2	+H	245.044 5	245.046 5	8.3	175.037 1, 147.044 4, 128.065 9, 91.059 2	花椒毒素+CO			+
M5	12.970	C₁₆H₁₆O₇	320.089 6	+H	321.096 9	321.096 2	-2.1	199.018 7, 175.077 1	欧前胡素-2H+2O/白花前胡甲素水解后单氧化	+	+	+
P17	13.452	C₁₄H₁₄O₅	262.084 1	+Na	285.073 3	285.073 9	1.8	225.170 4, 165.076 6, 153.074 1	芸香霉素	+	+	+	+	+	+
M6	13.740	C₁₄H₁₄O₅	262.084 1	+H	263.091 4	263.091 8	1.5	245.081 3, 203.069 9, 175.039 2, 147.044 9	白花前胡甲素C-3’, 4'双水解			+	+	+	+
M7	13.750	C₁₄H₁₂O₄	244.073 6	+H	245.080 8	245.081 0	0.5	227.070 0, 175.039 4, 175.039 2, 147.044 9, 128.063 5, 119.051 4, 91.057 4	白花前胡甲素C-3’, 4'双水解-H₂O			+
P19	15.022	C₁₄H₁₄O₄	246.089 2	+Na	269.078 4	269.078 1	-1.1	84.964 6	紫花前胡苷元			+			+
M8	15.678	C₁₂H₈O₄	216.042 3	+H	217.049 5	217.048 1	-6.7	68.987 1	欧前胡素-C₅H₈+CH₃			+
M9	17.25	C₁₀H₈O₃	176.047 3	+H	177.054 6	177.055 1	2.4	119.051 1, 99.009 8, 91.055 2, 77.044 8, 56.970 5	茵芋苷水解后+CH₃	+	+	+			+
M10	17.290	C₁₂H₁₀O₄	218.057 9	+H	219.065 2	219.065 4	0.9	177.053 1, 149.060 7, 119.050 5, 91.055 9	花椒毒素/佛手柑内酯氢化		+	+
M11	17.592	C₁₆H₁₅O₅	287.092 0	+H	288.099 2	288.099 7	1.6	246.043 4, 228.032 1, 182.028 3, 156.012 5, 131.991 7, 83.979 5	欧前胡素内水解	+	+	+
M12	17.690	C₁₆H₁₆O₆	304.094 7	+H	305.102 0	305.102 9	2.9	203.034 8, 175.033 9, 147.043 5	白花前胡甲素C-3’单水解(-C₄H₆CO)			+	+	+	+
P22	18.875	C₁₀H₈O₃	176.047 3	+H	177.054 6	177.054 9	1.3	117.038 7, 89.015 8	5-甲氧基香豆素	+	+	+			+
M13	19.542	C₁₁H₆O₄	202.026 6	+H	203.033 9	203.032 8	-5.1	175.033 0, 147.045 8, 101.953 0, 71.956 2	欧前胡素-C₅H₈/花椒毒素-CH₃	+	+
M14	19.550	C₂₀H₂₂O₁₀	422.121 3	+H	423.128 6	423.129 9	3.1	247.093 7, 191.034 1, 163.039 1, 135.045 8	紫花前胡苷元+Glu			+
P25	21.653	C₁₆H₁₈O₄	274.120 5	+Na	297.109 7	297.112 1	8.1	281.051 1, 265.021 2, 248.980 3, 73.051 0	九里香内酯	+
M15	24.450	C₂₅H₂₆O₁₂	518.142 4	+H	519.149 7	519.149 2	-0.9	343.121 0, 265.080 5, 251.070 9, 159.043 9	前胡香豆素E+Glu			+
P28	25.025	C₁₅H₁₆O₃	244.109 9	+H	245.117 2	245.117 3	0.3	227.070 7, 189.021 3, 175.039 6, 147.044 7, 131.050 7	蛇床子素	+			+
M16	25.960	C₁₆H₁₄O₆	302.079 0	+H	303.086 3	303.087 4	3.5	203.033 1, 147.043 5, 119.088 9, 103.052 9	欧前胡素+Di-oxidation			+	+
M17	26.208	C₂₂H₂₂O₁₀	446.121 3	+H	447.128 6	447.132 0	7.7	261.048 8, 243.042 8, 197.035 5, 56.011 9	欧前胡素+Glu	+	+	+	+	+	+
M18	26.220	C₂₀H₁₈O₉	402.095 1	+H	403.102 4	403.101 7	-1.5	385.096 3, 341.068 4, 297.035 9, 237.072 4, 193.052 6, 149.024 8	Arnocoumarin+Glu	+	+	+
M19	26.902	C₂₁H₂₂O₈	402.131 5	+Na	425.120 7	425.120 9	0.5	365.107 7, 215.064 8	Hydroxyl-dl praeruptorin A			+
M20	28.120	C₂₁H₂₂O₈	402.131 5	+Na	425.120 7	425.120 4	-0.6	365.092 9, 245.079 7, 227.067 2, 175.036 4	Hydroxyl-dl praeruptorin A			+		+	+
P29	28.141	C₁₆H₁₄O₅	286.084 1	+H	287.091 4	287.091 3	-0.3	245.159 4, 227.083 7, 175.087 9, 147.044 7	异氧化前胡素		+	+	+	+	+
M21	29.152	C₂₂H₂₈O₁₂	484.158 1	+H	485.165 4	485.168 3	6.1	240.087 3, 184.072 8	九里香素+Glu			+
M22	30.010	C₂₇H₃₂O₁₃	564.184 3	+H	565.191 6	565.196 5	8.6	389.153 3, 343.115 4, 243.064 6, 159.048 4, 83.053 7	白花前胡香豆精I+Glu			+
P33	30.380	C₁₁H₆O₃	186.031 7	+H	187.039 0	187.039 4	2.1	131.132 4, 115.300 2, 103.071 5	异补骨脂素		+		+	+	+
P34	31.059	C₁₉H₂₀O₆	344.126 0	+Na	367.115 2	367.115 4	0.4	315.215 7, 267.062 6, 203.070 6, 175.039 4	前胡香豆素A		+	+	+	+	+
M23	31.722	C₂₁H₂₂O₈	402.131 5	+Na	425.120 7	425.118 5	-5.2	365.102 6, 245.086 2, 227.069 2, 175.035 6	Hydroxyl-dl praeruptorin A			+	+	+	+
M24	32.840	C₁₈H₁₆O₁₀	392.074 4	+H	393.081 6	393.085 0	8.6	337.031 9, 204.075 1, 149.028 4	6-甲氧基当归素/花椒毒素+Glu	+	+	+
P39	34.013	C₁₉H₁₈O₆	342.110 3	+Na	365.099 6	365.100 8	3.3	265.049 0, 119.083 2	前胡香豆素E		+				+
P43	36.225	C₂₁H₂₂O₇	386.136 6	+Na	409.125 8	409.125 7	0.2	349.105 8, 327.123 2, 245.082 6, 227.071 3, 175.039 1, 128.062 8, 83.053 5	白花前胡甲素	+				+	+
P44	36.453	C₁₄H₁₀O₃	226.063 0	+H	227.070 3	227.070 8	2.5	199.075 5, 128.078 9	Arnocoumarin				+	+
P45	36.456	C₁₆H₁₄O₅	286.084 1	+Na	309.075 5	309.079 4	2.5	245.058 0, 193.032 4, 175.069 1, 147.062 7	氧化前胡素/欧前胡素氧化			+		+
P46	36.483	C₂₁H₂₂O₇	386.136 6	+Na	409.125 8	409.126 0	0.4	245.152 4, 227.089 2, 175.139 0	白花前胡香豆精Ⅱ		+	+	+	+
M25	37.050	C₁₉H₁₈O₅	326.115 4	+H	327.1227	327.123 0	0.8	245.079 6, 227.070 1, 199.075 3, 175.039 3, 128.063 3, 83.052 2	3'-Angeloyloxy-4'-hydroxyl-3′, 4'-dihydroseselin			+
P47	37.667	C₂₁H₂₄O₇	388.152 2	+Na	411.141 4	411.141 2	-0.6	141.068 5	白花前胡香豆精Ⅰ		+
P48	37.685	C₁₄H₂₂O	206.167 1	+H	207.174 3	207.175 7	6.7	189.169 4, 151.109 4	3, 5-二叔丁基苯酚	+
M26	39.020	C₂₅H₃₀O₇	442.199 2	+H	443.206 4	443.206 9	1	343.159 4, 301.106 8, 184.075 0, 83.049 0	白花前胡素E+CH₃	+	+	+
P50	41.467	C₂₄H₂₆O₇	426.167 9	+Na	449.1571	449.1568	-0.7	395.252 7, 319.199 3, 227.073 2, 119.084 4	白花前胡乙素			+		+
P51	42.556	C₂₄H₂₈O₇	428.183 5	+Na	451.1727	451.1718	-2.1	351.120 5, 245.087 5, 227.071 6, 175.036 7, 83.053 2	白花前胡丙素		+		+	+	+
P52	43.110	C₂₄H₂₈O₇	428.183 5	+Na	451.1727	451.1730	0.7	349.099 6, 245.080 8, 227.071 1, 175.039 8, 83.054 2	白花前胡素E		+	+		+	+
注: X/F-1、2、4为1、2、4 h前胡提取液给药大鼠血浆/肺部样品, 其后数值表示找到化合物的总个数; +.表示可检测到。

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