基于纳滤溶解扩散理论探索热毒宁注射液醇沉浓度对绿原酸分子态比例及传质行为的调控规律

章莲; 王智; 李存玉; 支兴蕾; 彭国平

doi:10.14148/j.issn.1672-0482.2022.0432

基于纳滤溶解扩散理论探索热毒宁注射液醇沉浓度对绿原酸分子态比例及传质行为的调控规律

doi: 10.14148/j.issn.1672-0482.2022.0432

章莲^1,,
王智¹,
李存玉^{1, 2, 3, 4, 5, ,},
支兴蕾¹,
彭国平^{1, 2, 3, 4, 5}

1.
南京中医药大学药学院, 江苏南京 210023
2.
江苏康缘药业股份有限公司, 江苏连云港 222001
3.
中药制药过程新技术国家重点实验室, 江苏连云港 222001
4.
江苏省经典名方工程研究中心，江苏南京 210023
5.
江苏省中药资源产业化过程协同创新中心，江苏南京 210023

基金项目:

江苏省自然科学基金面上项目 BK20211303

国家自然科学基金面上项目 82074006

中药制药过程新技术国家重点实验室开放基金项目 SKL2020Z0101

国家级大学生创新创业训练计划项目 202110315042

2021年度康缘中药学院创新创业项目 kyxysc06

详细信息

作者简介:
章莲, 女, 硕士研究生, E-mail: lian1439@qq.com

通讯作者:
李存玉, 男, 副教授, 主要从事纳滤分离机制及产业应用研究, E-mail: 300632@njucm.edu.cn

中图分类号: R284.1
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- 文章访问数: 220
- HTML全文浏览量: 41
- PDF下载量: 8
- 被引次数: 0
出版历程
- 收稿日期: 2021-11-19
- 发布日期: 2022-05-10

Exploring the Regulation Rules of Ethanol Precipitation Concentration on Chlorogenic Acid Molecular State and Nanofiltration mass Transfer Behavior in Reduning Injection Based on the Solution-Diffusion Theory

ZHANG Lian^1
,,
WANG Zhi¹,
LI Cun-yu^{1, 2, 3, 4, 5
, ,},
ZHI Xing-lei¹,
PENG Guo-ping^{1, 2, 3, 4, 5}

1.
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
2.
Jiangsu Kanion Parmaceutical Co. Ltd., Lianyungang 222001, China
3.
State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China
4.
Jiangsu Province Engineering Research Center of Classical Prescription, Nanjing 210023, China
5.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China

摘要

摘要: 目的研究热毒宁注射液醇沉浓度对绿原酸分子态比例及纳滤传质行为的调控规律。方法基于溶解-扩散效应, 考察热毒宁注射液醇沉浓度对绿原酸分子态比例的影响, 进而拟合传质系数与绿原酸浓度的相关性曲线, 分析乙醇浓度对传质行为的调控规律。结果纳滤传质数学模型可用于热毒宁注射液醇沉中间体溶液中的绿原酸存在状态定量分析, 醇沉浓度在60%~80%之间时, 以分子态形式存在的绿原酸比例在22.21%~82.43%范围内, 随着醇沉浓度升高, 绿原酸的主要存在状态由离子态、缔合态向分子态过渡, 迁移程度表现为80%醇沉>70%醇沉>60%醇沉。结论在高浓度乙醇溶液环境下, 绿原酸单体、60%及70%醇沉中间体的纳滤分离行为可通过“溶解扩散-孔道位阻”效应协同调控分离, 为有机溶液环境下的成分有序分离提供了研究思路。
- 绿原酸 /
- 热毒宁注射液 /
- 纳滤 /
- 溶解扩散 /
- 调控规律
Abstract: OBJECTIVE To study the regulation rules of ethanol precipitation concentration on chlorogenic acid molecular state and nanofiltration mass transfer behavior in Reduning injection. METHODS Based on the solution-diffusion effect, the regulation rules of chlorogenic acid molecular state and nanofiltration mass transfer behavior were calculated and analyzed by mass transfer mathematical model in ethanol precipitation intermediates of Reduning injection. RESULTS The experiment showed that the nanofiltration mass transfer model was applied to the quantitative analysis of chlorogenic acid in the ethanol precipitation intermediate solution of Reduning injection. The proportion of chlorogenic acid in molecular form ranged from 22.21% to 82.43% when the concentration of ethanol precipitation was between 60% and 80%. With the increase of ethanol concentration, the main existing states of chlorogenic acid gradually changed from ionic state, associative state to molecular state. Meanwhile, the migration degree was 80%>70%>60% of ethanol precipitation concentration, respectively. CONCLUSION The nanofiltration separation behavior of chlorogenic acid in monomer solution, 60% and 70% ethanol precipitation intermediates can be coordinated by the effect of "solution-diffusion and steric hindrance". This study provided a research road for the orderly separation of components in organic solution.
- chlorogenic acid /
- Reduning injection /
- nanofiltration /
- solution-diffusion /
- regulation rule

HTML全文

图 1 绿原酸ln[(1-R_o)·J_v/R_o]对J_v的相关性

Figure 1. The correlation of ln[(1-R_o)·J_v/R_o] and J_V of chlorogenic acid

下载: 全尺寸图片幻灯片

图 2 绿原酸纳滤传质系数与浓度的相关性曲线

Figure 2. Correlation curve between k and C₀ of chlorogenic acid

下载: 全尺寸图片幻灯片

图 3 绿原酸ln[(1-R_o)·J_v/R_o]对J_v的相关性

Figure 3. The correlation of ln[(1-R_o)·J_v/R_o] and J_V of chlorogenic acid

下载: 全尺寸图片幻灯片

图 4 绿原酸纳滤传质系数与浓度的相关性曲线

Figure 4. Correlation curve between k and C₀ of chlorogenic acid

下载: 全尺寸图片幻灯片

图 5 绿原酸ln[(1-R_o)·J_v/R_o]对J_V的相关性

Figure 5. The correlation of ln[(1-R_o)·J_v/R_o] and J_V of chlorogenic acid

下载: 全尺寸图片幻灯片

图 6 绿原酸纳滤传质系数与浓度的相关性曲线

Figure 6. Correlation curve between k and C₀ of chlorogenic acid

下载: 全尺寸图片幻灯片

图 7 乙醇浓度对绿原酸传质行为的影响

Figure 7. Effect of ethanol concentration on k of chlorogenic acid

下载: 全尺寸图片幻灯片

图 8 乙醇浓度对醇沉中间体传质行为的影响

Figure 8. Effect of ethanol concentration on k of ethanol precipitation intermediates

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表 1 60%醇沉溶液中绿原酸分子态比例

Table 1. Molecular form proportion of chlorogenic acid in precipitate solution with 60% ethanol

绿原酸溶液		60%醇沉溶液		分子态比例/%
浓度/ (mg·L^-1)	传质系数/ (10^-6 m·s^-1)	浓度/ (mg·L^-1)	传质系数/ (10^-6 m·s^-1)	分子态比例/%
24.67	1.56	56.01	1.48	22.21
150.13	1.93	70.01	1.52	25.78
246.32	2.06	112.02	1.59	25.17
399.63	2.08	280.05	1.84	36.94

下载: 导出CSV

表 2 70%醇沉溶液中绿原酸分子态比例

Table 2. Molecular form proportion of chlorogenic acid in precipitate solution with 70% ethanol

绿原酸溶液		70%醇沉溶液		分子态比例/%
浓度/ (mg·L^-1)	传质系数/ (10^-6 m·s^-1)	浓度/ (mg·L^-1)	传质系数/ (10^-6 m·s^-1)	分子态比例/%
25.36	1.03	74.70	1.18	70.12
150.00	1.43	93.37	1.23	69.58
274.67	1.62	149.40	1.33	65.24
399.53	1.76	373.50	1.46	42.34

下载: 导出CSV

表 3 80%醇沉溶液中绿原酸分子态比例

Table 3. Molecular form proportion of chlorogenic acid in precipitate solution with 80% ethanol

绿原酸溶液		80%醇沉溶液		分子态比例/%
浓度/ (mg·L^-1)	传质系数/ (10^-6 m·s^-1)	浓度/ (mg·L^-1)	传质系数/ (10^-6 m·s^-1)	分子态比例/%
25.365	0.96	83.61	1.17	82.43
149.72	1.26	104.51	1.19	71.81
274.26	1.35	167.22	1.21	48.80
399.77	1.50	418.06	1.25	22.99
525.35	1.54	-	-	-

下载: 导出CSV

表 4 乙醇浓度对绿原酸传质系数的贡献百分比

Table 4. Percentage contribution of ethanol concentration to k of chlorogenic acid

绿原酸浓度/(mg·L^-1)	60%→70%乙醇浓度/%	70%→80%乙醇浓度/%
25	88.88	11.12
150	71.64	28.36
275	64.95	35.05
400	57.85	42.15

下载: 导出CSV

表 5 乙醇浓度对醇沉中间体传质系数的贡献比例

Table 5. Percentage contribution of ethanol concentration to k of ethanol precipitation intermediates

绿原酸浓度/(mg·L^-1)	60%→70%乙醇浓度/%	70%→80%乙醇浓度/%
25	143.51	-43.51
150	78.16	21.84
275	70.39	29.61
400	64.85	35.15

下载: 导出CSV

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