二至丸对绝经后骨质疏松模型大鼠脂肪组织糖代谢影响

韩龙; 谭登; 万仕炜; 方彭华; 黄桂成; 闵文

doi:10.14148/j.issn.1672-0482.2022.0419

二至丸对绝经后骨质疏松模型大鼠脂肪组织糖代谢影响

doi: 10.14148/j.issn.1672-0482.2022.0419

南京中医药大学第一临床医学院, 江苏南京 210023

基金项目:

国家自然科学基金面上项目 81774335

国家自然科学基金面上项目 81803792

国家重点研究计划重点专项 2019YFC1709905

国家中医药管理局青年岐黄学者支持项目

详细信息

作者简介:
韩龙, 男, 硕士研究生，E-mail: longhan95@163.com

通讯作者:
闵文, 男，教授，主要从事中医药治疗骨伤科劳损和退变性疾病的临床及基础研究，E-mail: wenge1977@126.com

中图分类号: R285.5
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- 被引次数: 0
出版历程
- 收稿日期: 2021-05-21
- 发布日期: 2022-05-10

The Effect of Erzhiwan on Glucose Metabolism of Adipose Tissue in Postmenopausal Osteoporosis Model Rats

The First School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China

摘要

摘要: 目的探讨二至丸对绝经后骨质疏松症大鼠脂肪组织糖代谢的影响。方法通过切除双侧卵巢建立绝经后骨质疏松症大鼠模型, 大鼠随机分为: 模型组、阿仑膦酸钠组、氟化钠组和二至丸组, 每组8只。另设假手术组8只。分别予生理盐水(5 mL·kg^-1)，阿仑膦酸钠(1 mg·kg^-1)，氟化钠(5 mg·kg^-1)，二至丸(1.6 g·kg^-1)灌胃8周, 每日1次, 假手术组干预同模型组。采用ELISA法检测血清胰岛素，qPCR、Western blot法检测脂肪组织Glut4、PGC-1α、Sirt1 mRNA及蛋白表达。结果与假手术组相比, 模型组大鼠血清胰岛素明显增加(P < 0.05), 脂肪组织Glut4、PGC-1α及Sirt1 mRNA和蛋白表达水平显著减少(P < 0.01)。与模型组相比, 二至丸组血清胰岛素明显减少, 脂肪组织Glut4、PGC-1α及Sirt1 mRNA及蛋白表达水平显著增加(P < 0.01)。结论二至丸能够激活Sirt1/PGC-1α/Glut4通路，改善绝经后骨质疏松症状态下脂肪组织糖代谢紊乱。
- 二至丸 /
- 绝经后骨质疏松症 /
- 脂肪组织 /
- 糖代谢 /
- Sirt1/PGC-1α/Glut4通路
Abstract: OBJECTIVE To explore the effect of Erzhiwan on glucose metabolism of adipose tissue in postmenopausal osteoporosis model rats. METHODS The postmenopausal osteoporosis rat model was established by bilateral ovariectomy. All model rats were randomly divided into 4 groups: model group, alendronate sodium group, sodium fluoride group and Erzhiwan group, with 8 rats in each group. Another 8 rats were set in sham operation group. Each group was given normal saline (5 mL·kg^-1), alendronate sodium (1 mg·kg^-1), sodium fluoride(5 mg·kg^-1) and Erzhiwan (1.6 g·kg^-1) once a day for 8 weeks, respectively. The sham group was given the same intervention as the model group. Serum insulin was detected by ELISA. The expressions of Glut4, PGC-1α, Sirt1 mRNA and protein in adipose tissue were detected by qPCR and Western blot. RESULTS Compared with the sham group, the serum insulin in the model group increased significantly, and the mRNA and protein expressions of Glut4, PGC-1α and Sirt1 in adipose tissue decreased significantly. Compared with the model group, the serum insulin in Erzhiwan group decreased significantly, and the mRNA and protein expressions of Glut4, PGC-1α and Sirt1 in adipose tissue increased significantly. CONCLUSION Erzhiwan can activate Sirt1/PGC-1α/Glut4 to improve the disorder of glucose metabolism in adipose tissue under postmenopausal osteoporosis.
- Erzhiwan /
- postmenopausal osteoporosis /
- adipose tissue /
- glucose metabolism /
- Sirt1/PGC-1α/Glut4 pathway

HTML全文

图 1 大鼠体质量及内脏脂肪质量比较

注: Sham.假手术组；Model.模型组；AL.阿仑膦酸钠组；NAF.氟化钠组；Erzhiwan.二至丸组。与假手术组相比，^#P < 0.05, ^##P < 0.01。

Figure 1. Comparison of body weight and visceral fat weight in each group

下载: 全尺寸图片幻灯片

图 2 二至丸对大鼠骨密度的影响

注: Sham.假手术组；Model.模型组；AL.阿仑膦酸钠组；NAF.氟化钠组；Erzhiwan.二至丸组。与假手术组相比，^##P < 0.01;与模型组相比，^**P < 0.01。

Figure 2. Effect of Erzhiwan on bone density in rats

下载: 全尺寸图片幻灯片

图 3 二至丸对大鼠FBG、GTT和AUC指数的影响

注: Sham.假手术组；Model.模型组; AL.阿仑膦酸钠组; NAF.氟化钠组; Erzhiwan.二至丸组。与假手术组相比，^##P < 0.01;与模型组相比，^*P < 0.05, ^**P < 0.01。

Figure 3. Effects of Erzhiwan on FBG, GTT and AUC in rats

下载: 全尺寸图片幻灯片

图 4 二至丸对大鼠血清胰岛素水平的影响

注: Sham.假手术组；Model.模型组；AL.阿仑膦酸钠组；NAF.氟化钠组；Erzhiwan.二至丸组。与假手术组相比，^#P < 0.05;与模型组相比，^*P < 0.05, ^{* *}P < 0.01。

Figure 4. Effect of Erzhiwan on serum insulin in rats

下载: 全尺寸图片幻灯片

图 5 二至丸对大鼠脂肪Glut4、PGC-1α、Sirt1 mRNA表达的影响

注: Sham.假手术组；Model.模型组；AL.阿仑膦酸钠组；NAF.氟化钠组；Erzhiwan.二至丸组。与假手术组相比，^#P < 0.05, ^##P < 0.01; 与模型组相比，^*P < 0.05, ^{* *}P < 0.01。

Figure 5. Effects of Erzhiwan on mRNA expression of Glut4, PGC-1α and Sirt1 in rat adipose tissue

下载: 全尺寸图片幻灯片

图 6 二至丸对大鼠脂肪组织Glut4、PGC-1α、Sirt1蛋白表达的影响

注: Sham.假手术组；Model.模型组；AL.阿仑膦酸钠组；NAF.氟化钠组；Erzhiwan.二至丸组。与假手术组相比，^#P < 0.05, ^##P < 0.01;与模型组相比，^*P < 0.05, ^**P < 0.01。

Figure 6. Effects of Erzhiwan on proteins expression of Glut4, PGC-1α and Sirt1 in rat adipose tissue

下载: 全尺寸图片幻灯片

表 1 引物序列

Table 1. Primer sequences

引物名称	序列
Glut4(F)	5'-AGGCCGGGACACTATACCC-3'
Glut4(R)	5'-ACTTTCTGTGGGGCGTTGAT-3'
PGC-1α(F)	5'-GCACCAGAAAACAGCTCCAA-3'
PGC-1α(R)	5'-TTACTGAAGTTGCCATCCCG-3'
Sirt1(F)	5'-AGGGAACCTCTGCCTCATCT-3'
Sirt1(R)	5'-GAGGTGTTGGTGGCAACTCT-3'
β-actin(F)	5'-GGCTGTGTTGTCCCTGTATG-3'
β-actin(R)	5'-AATGTCACGCACGATTTCC-3'

下载: 导出CSV

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