Metabolic Characteristics of Spleen-Qi Deficiency Syndrome in Ulcerative Colitis
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摘要: 目的 基于非靶标代谢组学探索溃疡性结肠炎(UC)脾气虚证的生物学基础。方法 采用液相色谱质谱联用方法对UC脾气虚证和湿热证患者血清进行代谢组学分析。结果 UC脾气虚证存在脂质代谢紊乱, 与UC湿热证患者比较, UC脾气虚证血清中1-油酰基甘油磷酸胆碱、溶血磷脂、鞘氨醇、N-棕榈酰磷酸乙醇胺、棕榈酰肉碱、O-花生四烯酰缩水甘油等代谢产物丰度降低, 2-亚油酰甘油丰度升高。ROC曲线结果显示, O-花生四烯酰缩水甘油、棕榈酰胺、2-亚油酰甘油可能是诊断UC脾气虚证的潜在生物标志物。结论 代谢组学方法有望揭示UC中医证候的生物学本质, 为UC中医辨证提供一定客观依据。Abstract: OBJECTIVE To explore the biological basis of spleen-qi deficiency syndrome of ulcerative colitis based on non-target metabolomics.METHODS The UC patients with spleen-qi deficiency syndrome and damp-heat syndrome were analyzed by metabolomics by LC-MS.RESULTS There were lipid metabolism disorders in UC patients with spleen-qi deficiency. Compared with UC patients with damp-heat, the average abundance of 1-linoleoylglycerophosphocholine, lysophospholipid, sphingosine, N-palmitoyl-phosphoethanolamine, palmitoylcarnitine and O-arachidonoyl glycidol decreased in the UC patients with spleen-qi deficiency, while the average abundance of 2-linoleoyl glycerol increased. ROC curve showed that O-arachidonoyl glycidol, palmitic amide and 2-linoleoyl glycerol may be potential biomarkers of spleen-qi deficiency syndrome in UC.CONCLUSION Metabolomics is expected to reveal the biological nature of TCM syndromes in UC.
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Key words:
- ulcerative colitis /
- spleen-qi deficiency /
- metabolomics
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图 3 溃疡性结肠炎不同证型患者血清差异代谢物
注: HC.健康对照组; PX.UC脾气虚证组; SR.UC湿热证组; 与HC组比较，#P < 0.05，##P < 0.01；与PX组比较，*P < 0.05，**P < 0.01。
图 4 差异代谢物的ROC曲线图
注: A~C.O-花生四烯酰缩水甘油、棕榈酰胺、2-亚油酰甘油在UC脾气虚证中的ROC曲线图; D~F.O-花生四烯酰缩水甘油、棕榈酰胺、2-亚油酰甘油在UC湿热证中的ROC曲线图
表 1 2组基线资料比较(x±s)
组别 性别 年龄/岁 BMI 男 女 健康对照组(n=27) 8 19 44.3±10.91 21.35±2.91 UC湿热证组(n=30) 17 13 42.13±12.41 23.11±3.34 UC脾气虚证组(n=31) 18 13 38.77±8.50 20.48±3.96 
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表 2 疾病特征
组别 病程/ a 直肠型 左半结肠型 广泛结肠型 血沉/ (mm·h-1) C反应蛋白/ (mg·L-1) 粪钙卫蛋白/ (μg·g-1) UC湿热证(n=30) 5.00±0.93 4 10 16 26.52±5.36 18.88±5.92 671.84±125.65 UC脾气虚证(n=31) 4.62±0.72 2 9 20 21.52±4.20 7.52±2.43 439.2±70.60
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