腺苷介导循环机械按压内关对心肌缺血大鼠的保护效应

徐森磊; 顾任钧; 杨雪莹; 卞向宇; 徐忻; 夏雪峰; 孙珂; 吴嘉宏; 刘雨晨; 卢圣锋; 张宏如; 顾一煌

doi:10.14148/j.issn.1672-0482.2021.0682

腺苷介导循环机械按压内关对心肌缺血大鼠的保护效应

doi: 10.14148/j.issn.1672-0482.2021.0682

徐森磊^1,,
顾任钧²,
杨雪莹¹,
卞向宇¹,
徐忻¹,
夏雪峰¹,
孙珂¹,
吴嘉宏¹,
刘雨晨¹,
卢圣锋^{1, 3},
张宏如^{1, 4},
顾一煌^1, ,

1.
南京中医药大学针灸推拿学院·养生康复学院, 江苏南京 210023
2.
南京中医药大学第二附属医院/江苏省第二中医院, 江苏南京 210027
3.
南京中医药大学针药结合教育部重点实验室, 江苏南京 210023
4.
南京中医药大学中医学院·中西医结合学院, 江苏南京 210023

基金项目:

国家自然科学基金 81974583

国家自然科学基金 81704169

南京中医药大学护理学优势学科第三期开放课题 2019YSHL044

江苏中医药领军人才 SLJ0226

详细信息

作者简介:
徐森磊, 男, 博士研究生，E-mail：gravity_xu@hotmail.com

通讯作者:
顾一煌, 男，教授，主要从事电针抗心肌缺血损伤的机制研究，E-mail：gyh5196411@163.com

中图分类号: R285.5
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- 被引次数: 0
出版历程
- 收稿日期: 2021-05-30
- 网络出版日期: 2021-12-21
- 刊出日期: 2021-09-10
- 发布日期: 2021-09-15

Adenosine-Mediated Cardioprotection of Local Cyclical Compression on Neiguan on Myocardial Ischemic Rats

1.
School of Acupuncture and Tuina, School of Regimen and Rehabilitation, Nanjing, 210023, China
2.
The Second Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Provincial Second Traditional Chinese Medicine Hospital, Nanjing, 210027, China
3.
Key Laboratory for Researching Combination of Acupuncture and Drugs of Ministry of Education, Nanjing, 210023, China
4.
School of Chinese Medicine, School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China

摘要

摘要: 目的观察循环机械按压内关对心肌缺血大鼠心肌梗死面积和心功能的影响, 初步研究机械因素产生心肌保护效应的机制。方法 18只成年雄性SPF级SD大鼠随机分为假手术组、模型组和机械刺激组, 每组6只。模型组和机械刺激组通过结扎大鼠冠状动脉左前降支建立心肌缺血模型, 假手术组只穿刺心脏不结扎。机械刺激组采用自制仪器按压内关, 按压面为直径3 mm圆形硅胶头, 力度为150 g, 按压方式为按压5 min, 放松5 min, 3次循环为1组, 每天干预1次, 连续3 d。机械刺激3 d后, 三苯基氯化四氮唑(TTC)染色测定心肌梗死面积; 超声心动图测定左室射血分数(LVEF); Western blot法检测大鼠心肌中炎症因子白介素-1β(IL-1β)、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的表达; ELISA法检测血清腺苷的浓度; 免疫组化法检测穴区腺苷脱氨酶(ADA)的表达水平。结果与假手术组比, 模型组大鼠心肌梗死面积增加(P < 0.001), LVEF值下降(P < 0.001), 血清中腺苷含量和穴区ADA的表达没有差异(P>0.05);与模型组比较, 机械刺激组大鼠心肌梗死面积减少(P < 0.01), LVEF值增加(P < 0.001), 心肌中炎症因子IL-1β、IL-6和TNF-α的表达显著降低(P < 0.000 1), 血清中腺苷含量增加(P < 0.01), 穴区腺苷脱氨酶表达水平减少(P < 0.05)。结论循环机械按压内关穴可减少心肌缺血损伤大鼠的梗死面积, 改善心功能; 其机制或与机械刺激抑制穴区ADA表达, 限制腺苷降解, 进而提高动脉中的腺苷浓度有关。
- 心肌缺血 /
- 机械刺激 /
- 内关穴 /
- 腺苷 /
- 腺苷脱氨酶
Abstract: OBJECTIVE To observe the effect of cyclic mechanical compression of Neiguan (PC 6) on myocardial infarct size and cardiac function in rats with myocardial ischemia, and to preliminarily investigate the possible mechanism by which this factor produces cardioprotective effects.METHODS A total of 18 adult male SD rats were randomly divided into sham, model, and mechanical stimuli group, 6 rats in each group. The myocardial ischemia model was established by ligating the left anterior descending (LAD) branch of the coronary artery in the model and mechanical stimuli group, while threading but not ligating at LAD was applied in the sham group. In the mechanical stimuli group, the Neiguan (PC 6) was pressed by the self-made instrument, with a contact surface of 3 mm diameter circular silica gel head and a force of 150 g, and the pressing mode was 5 min of pressing and 5 min of relaxation, with 3 cycles as one group, with intervention once a day for 3 consecutive days. The TTC staining was used to measure myocardial infarction area. Left ventricular ejection fraction(LVEF)was measured by echocardiography. The myocardial expression of IL-1β, IL-6 and TNF-α were detected by Western blot. Serum adenosine concentration was detected by ELISA. The expression level of adenosine deaminase(ADA)in acupoints was detected by immunohistochemistry.RESULTS Compared with sham group, myocardial infarction area of model group increased(P < 0.001), LVEF value decreased(P < 0.001), and there was no difference in serum adenosine content and ADA expression in acupoint area (P>0.05). Compared with model group, the myocardial infarction area and myocardial expression of IL-1β, IL-6 and TNF-α in mechanical stimuli group decreased (P < 0.01), LVEF value increased (P < 0.001), serum adenosine content increased (P < 0.01), and the expression level of adenosine deaminase in acupoints decreased (P < 0.05).CONCLUSION Cyclic mechanical compression of Neiguan (PC 6) can reduce the infarct size and improve cardiac function in Myocardial ischemic rats. The mechanism may be related to mechanical stimuli limiting adenosine degradation by inhibiting the expression of ADA in acupoint, contributing to cardioprotection via increased adenosine concentration in the artery.
- myocardial ischemia /
- mechanical stimuli /
- neiguan /
- adenosine /
- adenosine deaminase

HTML全文

图 1 造模前后大鼠心电图Ⅱ导联比较

下载: 全尺寸图片幻灯片

图 2 各组大鼠心肌梗死面积百分比的比较

注: 与模型组比较, ^{* * *}P < 0.001，^{* * * *}P < 0.000 1。x±s, n=6。

下载: 全尺寸图片幻灯片

图 3 各组大鼠超声心动图及射血分数比较

注: 与模型组比较, ^{* * * *}P < 0.000 1。x±s, n=6。

下载: 全尺寸图片幻灯片

图 4 各组大鼠心肌中炎症因子IL-1β、IL-6和TNF-α的表达水平比较

注: 与模型组比较, ^{* * * *}P < 0.000 1。x±s, n=3。

下载: 全尺寸图片幻灯片

图 5 各组大鼠血清中腺苷的表达水平比较

注: 与模型组比较, ^{* *}P < 0.01。x±s, n=3。

下载: 全尺寸图片幻灯片

图 6 各组大鼠内关穴区皮肤中ADA表达水平比较

注: 与模型组比较, ^**P < 0.01。x±s, n=3。

下载: 全尺寸图片幻灯片

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