The Chemical Constituents and Application Prospects on Wound Healing of Lasiosphaera Seu Calvatia
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摘要: 马勃Lasiosphaera Calvatia属于真菌类中药,具有消肿、止血、解毒等功效,常以粉末形式外治创伤出血。古籍记载及现代临床发现马勃具有治疗慢性难愈性创面的功效,为深入研究马勃治疗慢性难愈性创面的活性物质基础和药理作用及机制,作者对近年来国内外有关脱皮马勃Lasiosphaera fenzlii Reich.、大马勃Calvatia gigantea(Batsch ex Pers.)Lloyd和紫色马勃Calvatia lilacina(Mont.et Berk.)Lloyd的报道进行综述,整理出超过100种化学成分,包括甾体类化合物、蛋白质及多肽、氨基酸、脂肪酸及酯、糖类及挥发性成分等。马勃具有止血、抗氧化、抑菌、降血糖、促进细胞增殖等药理作用,通过调控创面微环境,促进增殖期进程,恢复慢性难愈性创面愈合。同时提出马勃在药材应用中存在的问题及解决方法,展望马勃在创面修复治疗的应用前景,以期为马勃资源在临床创伤治疗领域的合理开发提供理论依据。Abstract: Lasiosphaera seu Calvatia belongs to fungal traditional Chinese medicine, which has the effects of detumescence, hemostasis and detoxification, and is often used to treat traumatic bleeding in the form of powder. Ancient books and modern clinical records have found that Lasiosphaera seu Calvatia has the effect of treating chronic refractory wounds. Aiming to investigate the active material basis, pharmacological action and mechanism of Lasiosphaera seu Calvatia in the treatment of chronic refractory wounds in the present articals, the authors reviewed the recent reports on peeling Lasiosphaera fenzlii Reich., Calvatia gigantea (Batsch ex Pers.) Lloyd and Calvatia lilacina (Mont.et Berk.) Lloyd at home and abroad, and sorted out more than 100 types of chemical constituents, including steroids, proteins and peptides, amino acids, fatty acids and esters, sugars and volatile components, etc.The main pharmacological effects are reported for hemostasis, anti-inflammation, antioxidation, bacteriostasis, hypoglycemia, promoting cell proliferation and etc. By regulating wound microenvironment, promoting the process of cell proliferation, restoring chronic refractory wound healing, Lasiosphaera seu Calvatia is expected to be used as medicinal materials and utilized in wound repair treatment, in order to provide a theoretical basis for the rational development and utilization of Lasiosphaera seu Calvatia resources in the field of clinical trauma treatment.
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表 1 马勃化学成分
编号 分类 化合物名称 品种 部位 参考文献 1 甾体类化合物 麦角甾醇(Ergosterol) A、B、C a、b [8, 35-36] 2 麦角甾-7, 22-二烯-3-醇(Ergosta-7, 22-dien-3-ol) A a、b [35] 3 (22E, 24R)-麦角甾-7, 22-二烯-3β-醇[(22E, 24R)-Ergosta-7, 22-dien-3β-ol] A a [35] 4 麦角甾-5, 7, 22-三烯-3-醇(Ergosta-5, 7, 22-trien-3-ol) A a [35] 5 麦角甾-7, 22-二烯-3β, 5α, 6β-三醇(Ergosta-7, 22-diene-3β, 5α, 6β-triol) A - [36] 6 麦角甾-7, 22-二烯-3β-酮(Ergosta-7, 22-dien-3-one) A、B a、b [35-37] 7 羟基麦角甾-7, 22-二烯-6-酮(3-Hydroergosta-7, 22-dien-6-one) A - [35] 8 8β-羟基麦角甾-4, 6, 22-三烯-3-酮(8β-Hydroergosta-4, 6, 22-triene-3-one) A - [35] 9 (22E, 24R)-麦角甾-4, 6, 8(14), 22-四烯-3-酮[(22E, 24R)-Ergosta- 4, 6, 8(14), 22-tetraen-3-one] A a [37] 10 麦角甾-7, 22-二烯-3, 6-二酮(Ergosta-7, 22-dien-3, 6-dione) A a [35] 11 麦角甾-4, 7, 22-三烯-3, 6-二酮(Ergosta-4, 7, 22-triene-3, 6-dione) A - [35] 12 5α, 8α-环二氧-(22E, 24E)-麦角甾-6, 22-二烯-3β-醇[5α, 8β-Epidioxy- (22E, 24E)-ergosta-6, 22-dien-3β-ol] A a [35, 37] 13 [7, 7'-Biergosta-4, 22-diene]-3, 6, 3', 6'-tetraone-8, 8'-dihydroxy A - [35] 14 β-谷甾醇(β-Sitosterol) A、B a [37-39] 15 氨基酸 苯丙氨酸(Phenylalanine) A、B a [14, 36] 16 色氨酸(Tryptophan) B a [14] 17 异亮氨酸(Isoleucine) B a [14] 18 缬氨酸(Valine) B a [14] 19 亮氨酸(Leucine) B a [14] 20 苏氨酸(Threonine) B a [14] 21 赖氨酸(Lysine) B a [14] 22 组氨酸(Histidine) B a [14] 23 蛋氨酸(Methionine) B a [14] 24 酪氨酸(Tyrosine) B a [14] 25 4-羟基脯氨酸(4-Hydroxyproline) B a [14] 26 精氨酸(Arginine) B a [14] 27 脯氨酸(Proline) B a [14] 28 甘氨酸(Glycine) B a [14] 29 丝氨酸(Serine) B a [14] 30 丙氨酸(Alanine) B a [14] 31 谷氨酰胺(Glutamine) B a [14] 32 谷氨酸(Glutamic acid) B a [14] 33 天冬氨酸(Aspartic acid) B a [14] 34 其他小分子含氮化合物 4, 6-二羟基异吲哚-1-酮(4, 6-Dihydroxyisoindol-1-one) A a [38] 35 4, 6-二羟基-异吲哚-1, 3-二酮(4, 6-Dihydroxy-isoindol-1, 3-dione) A a [38] 36 ClitocybinA A a [38] 37 脂肪酸及酯 亚油酸(Linoleic acid) A、B a [18, 40] 38 棕榈酸(Palmitic acid) A、B a [18, 35-36, 39] 39 油酸(Oleic acid) B a [18] 40 硬脂酸(Stearic acid) A a、b [35] 41 2, 3-二羟丙基油酸酯(2, 3-Dihydroxypropyl oleate) A a [37] 42 十六烷酸乙酯(Palmitic acid ethyl ester) A、B、C a [41] 43 4-羟基苯基乙酸酯(4-Hydroxyphenylacetate) A - [36] 44 棕榈酸胆甾烯酯(Palmitatic acid cholesteryl ester) B a [39] 45 油酸乙酯(Ethyl oleate) A、B [41] 46 雪松醇(Cedrol) B a [42] 47 α-石竹烯(α-Caryophyllene) A、B a [42-43] 48 氧化石竹烯(Caryophyllene oxide) B a [42-43] 49 2, 2'-亚甲基双-(4-甲基-6-叔丁基苯酚)[2, 2'-Methylenebis (4-methyl-6-tert-butylphenol)] A、B、C a [41] 50 7-Amino-1, 4-dimethylpyrimido[4, 5-c]pyridazine-3, 5-(1H, 2H)-dione A、B、C a [41] 51 2-乙烯基呋喃(2-Vinylfuran) A a [43] 52 甲苯(Toluene) A a [43] 53 2-庚酮(2-Heptanone) A a [43] 54 庚醛(Heptanal) A a [43] 55 苯甲醛(Benzaldehyde) A a [18, 43] 56 二甲基三硫(Trisulfide,dimethy) A a [43] 57 庚醇(n-Heptanol) A a [43] 58 1-辛烯-3-醇(1-Octen-3-ol) A、B a [18, 43] 59 苯甲腈(Benzonitrile) A a [43] 60 2-正戊基呋喃(2-Pentylfuran) A a [43] 61 正辛醛(Octanal) A a [43] 62 双戊烯(Limonene) A a [43] 63 3-辛烯-2-酮(3-Octen-2-one) A a [43] 64 苯乙醛(Hyacinthin) A a [43] 65 苯乙酮(Acetophenone) A a [43] 66 正辛醇(1-Octanol) A a [43] 67 壬醛(Pelargonalde) A a [43] 68 樟脑醑(Camphor) A a [43] 69 1-壬醇(1-Nonanol) A a [43] 70 4-萜烯醇(Terpinene-4-ol) A a [43] 71 α-松油醇(α-Terpineol) A a [43] 72 2, 6-二氯苯甲醚[2, 6-Dichloranisole] A a [43] 73 2-癸烯醛(2-Decenal) A a [43] 74 2-十一酮(2-Undecanone) A a [43] 75 1-甲基萘(α-Methylnaphthalene) A a [43] 76 百里香酚(Thynol) A a [43] 77 2, 4-癸二烯醛(2, 4-Decadienal) A a [43] 78 δ-榄香烯(δ-Elemene) A a [43] 79 甲位依兰烯(α-Ylangene) A a [43] 80 α-蒎烯(α-Copaene) A a [43] 81 榄香烯(β-Elemene) A a [43] 82 甲基丁香酚(Methyl eugenol) A a [43] 83 β-石竹烯(β-Caryophyllene) A a [43] 84 1-苯基-3-己酮(1-Phenyl-3-hexanone) A a [43] 85 β-荜澄茄油萜(β-Cubebene) A a [43] 86 (+)-香橙烯[(+)-Aromadendrene] A a [43] 87 十二醇(1-Dodecanol) A a [43] 88 芳-姜黄烯(Ar-curcumene) A a [43] 89 β-芹子烯(β-Selinene) A a [43] 90 苊(Acenaphthylene) A a [43] 91 β-没药烯(β-Bisabolene) A a [43] 92 β-杜松萜烯(β-Cadinene) A a [43] 93 α-甜旗烯(α-Calacorene) A a [43] 94 橙花叔醇(Nerolidol) A a [43] 95 白菖油萜(Calarene) A a [43] 96 β-姜黄酮(β-Tumerone) A a [43] 97 乙基苯(Benzene, ethyl) - a [41] 98 苯乙烯(Styrene) - a [41] 99 4-乙基-3-甲基吡啶(4-Ethyl-3-methylpyridine) - a [41] 100 4-苯乙炔基苯乙酮(4-Phenylethynyl-acetophenone) A、C a [41] 101 bis-, cis-1, 2-二苯基环丁烷[Benzene, 1, 1'-(1, 2-cyclobutanediyl)] - a [41] 102 5, 6-二氢-5, 6-二甲吩嗪(5, 6-Dihydro-5, 6-dimethylbenzo[c]cinnoline) - a [41] 103 2, 4-二苯基-1-丁烯(2, 4-Diphenyl-1-butene) - a [41] 104 顺式-1, 2-二苯基环丁烷(Cyclobutene, 1, 2-diphenyl) - a [41] 105 4-乙炔联苯(4-Ethynylbiphenyl) - a [41] 106 邻苯二甲酸二丁酯(1, 2-Benzenedicarboxylic acid-dibutyl ester) - a [41] 107 二十二烷(Docosane) A a [41] 108 二十烷(Eicosane) A a [41] 109 十八烷(Octadecane) - a [41] 110 2-Aza-4, 5, 9, 10-tetrahydropyrene - a [41] 111 (3S, 4Z)-Dodeca-4, 11-dien-1-yn-3-ol B a [41] 112 3, 4-二甲基苯胺(Benzenamine, 3, 4-dimethyl) A、B、C a [41] 113 6-溴-5-甲氧基吲哚(6-Bromo-5-methoxyindole) A a [41] 114 n-Propyl-9, 12-octadecadienoate A、B a [41] 115 9-炔十八酸甲酯[9-Octadecenoic acid(Z)-methyl ester] C a [41] 116 10-十八烯酸甲酯(10-Octadecenoic acid, methyl ester) A a [41] 117 Racemosol A、C a [41] 118 Cubenol C a [41] 119 衣兰烯(α-Ylangene) B a [41] 120 3-(Dimethyphenysilyl)-2-methylpropionamide B a [41] 121 (E)-3', 4'-(Methylenedioxy) dihydrocinnamaldehyde B a [41] 122 3, 6-Heptanooxepin-4, 5-dicarbonsaure-dimethylester A a [41] 123 十九烷(Nonadecane) A a [41] 124 Phenol, 2, 2'-methylenebis[6-(1, 1-dimethylethy)]-4-methyl 2, 2'-亚甲基双-(4-甲基-6-叔丁基苯酚) A、B、C a [41] 125 其他 苯甲酸(Benzoic acid) A - [40] 126 对羟基苯甲酸(4-Hydroxybenzoic acid) A、B b [18, 36] 127 原儿茶酸(3, 4-Dihydroxybenzoic acid) B - [18] 128 龙胆酸(2, 5-Dihydroxybenzoic acid) B - [18] 129 3, 5-二羟基苯甲酸(3, 5-Dihydroxybenzoic acid) A a [38] 130 对香豆酸(4-Hydroxycinnamic acid) A、B - [18, 36] 131 4-羟甲基-2-糠醛(4-Hydroxymethyl-2-furaldehyde) A a [38] 132 2-戊基呋喃(2-Pentylfuran) B - [18] 133 4, 6-二羟基-1(3H)-异苯并呋喃酮[4, 6-Dihydroxy-1(3H)-isobenzofuranone] A a [38] 134 5, 7-二羟基-1(3H)-异苯并呋喃酮[5, 7-Dihydroxy-1(3H)-isobenzofuranone] A a [38] 135 D-阿洛醇(D-Allitol) A a、b [37] 136 对苯二酚(Hydroquinone) A a [36] 137 反式桂皮醇(Trans-cinnamic alcohol) A - [36] 138 己醛(Hexanal) B - [18] 注:A.脱皮马勃Lasiosphaera fenzlii Reich.; B.大马勃Calvatia gigantea(Batsch ex Pers.)Lloyd; C.紫色马勃Calvatia lilacina(Mont.et Berk.)Lloyd; a.子实体;b.菌丝体发酵液; “-”表示该化合物的来源或部位不明确。 -
[1] WU YK, CHENG NC, CHENG CM. Biofilms in chronic wounds: Pathogenesis and diagnosis[J]. Trends Biotechnol, 2019, 37(5): 505-517. doi: 10.1016/j.tibtech.2018.10.011 [2] 修一平, 张丽艳, 钱学义, 等. 自体富血小板血浆修复慢性难愈性创面的作用: 回顾性研究和文献检索证据分析[J]. 中国组织工程研究, 2020, 24(8): 1231-1237. https://www.cnki.com.cn/Article/CJFDTOTAL-XDKF202008017.htm [3] JARBRINK K, NI G, SONNERGREN H, et al. Prevalence and incidence of chronic wounds and related complications: A protocol for a systematic review[J]. Syst Rev, 2016, 5(1): 152. doi: 10.1186/s13643-016-0329-y [4] KATHAWALA MH, NG WL, LIU D, et al. Healing of chronic wounds: An update of recent developments and future possibilities[J]. Tissue Eng Part B Rev, 2019, 25(5): 429-444. doi: 10.1089/ten.teb.2019.0019 [5] 国家药典委员会. 中华人民共和国药典: 四部[M]. 北京: 中国医药科技出版社, 2020: 52. [6] 陶弘景. 名医别录[M]. 尚志钧, 辑校. 北京: 人民卫生出版社, 1986. [7] 吴仪洛. 本草从新[M]. 朱建平, 吴文清, 点校. 北京: 中医古籍出版社, 2001: 254. [8] 相聪坤, 宿树兰, 关胜江, 等. HPLC法测定不同产地不同品种马勃药材中麦角甾醇[J]. 中草药, 2016, 47(8): 1397-1400. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO201608029.htm [9] 陈晗, 陈丹倩, 李全福, 等. 麦角甾酮的药理活性、药代动力学及含量测定研究进展[J]. 中国中药杂志, 2014, 39(20): 3905-3909. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201420004.htm [10] 麻兵继, 文春南, 吴婷婷, 等. 麦角甾醇过氧化物的抑菌活性研究[J]. 食品研究与开发, 2012, 33(7): 42-44. doi: 10.3969/j.issn.1005-6521.2012.07.013 [11] LIAO PC, LAI MH, HSU KP, et al. Identification of β-sitosterol as in vitro anti-inflammatory constituent in Moringa oleifera[J]. J Agric Food Chem, 2018, 66(41): 10748-10759. doi: 10.1021/acs.jafc.8b04555 [12] 蒋琼凤, 袁志辉, 李进, 等. 蓼蓝中β-谷甾醇的提取及其抗氧化性研究[J]. 食品工业科技, 2015, 36(6): 108-112. https://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201506033.htm [13] DING K, TAN YY, DING Y, et al. β-Sitosterol improves experimental colitis in mice with a target against pathogenic bacteria[J]. J Cell Biochem, 2019, 120(4): 5687-5694. doi: 10.1002/jcb.27853 [14] KIVRAK I, KIVRAK S, HARMANDAR M. Free amino acid profiling in the giant puffball mushroom (Calvatia gigantea) using UPLC-MS/MS[J]. Food Chem, 2014, 158: 88-92. doi: 10.1016/j.foodchem.2014.02.108 [15] TACK C, SHORTHOUSE F, KASS L. The physiological mechanisms of effect of vitamins and amino acids on tendon and muscle healing: A systematic review[J]. Int J Sport Nutr Exerc Metab, 2018, 28(3): 294-311. doi: 10.1123/ijsnem.2017-0267 [16] HUNG SY, TSAI JS, YEH JT, et al. Amino acids and wound healing in people with limb-threatening diabetic foot ulcers[J]. J Diab Compl, 2019, 33(10): 107403. doi: 10.1016/j.jdiacomp.2019.06.008 [17] AYDIN H, TATAR C, SAVAS OA, et al. The effects of local and systemic administration of proline on wound healing in rats[J]. J Investig Surg, 2019, 32(6): 523-529. doi: 10.1080/08941939.2018.1441342 [18] KIVRAK I, KIVRAK S, HARMANDAR M. Bioactive compounds, chemical composition, and medicinal value of the giant puffball, Calvatia gigantea (higher basidiomycetes), from Turkey[J]. Int J Med Mushrooms, 2016, 18(2): 97-107. doi: 10.1615/IntJMedMushrooms.v18.i2.10 [19] POLJSAK N, KREFT S, KOCEVAR GLAVAC N. Vegetable butters and oils in skin wound healing: Scientific evidence for new opportunities in dermatology[J]. Phytother Res, 2020, 34(2): 254-269. doi: 10.1002/ptr.6524 [20] JARA CP, MENDES NF, PRADO TPD, et al. Bioactive fatty acids in the resolution of chronic inflammation in skin wounds[J]. Adv Wound Care, 2020, 9(8): 472-490. doi: 10.1089/wound.2019.1105 [21] 尚庆辉, 解玉怀, 张桂国, 等. 植物多糖的免疫调节作用及其机制研究进展[J]. 动物营养学报, 2015, 27(1): 49-58. doi: 10.3969/j.issn.1006-267x.2015.01.008 [22] YANG Y, WANG F, YIN D, et al. Astragulus polysaccharide-loaded fibrous mats promote the restoration of microcirculation in/around skin wounds to accelerate wound healing in a diabetic rat model[J]. Colloids Surf B Biointerfaces, 2015, 136: 111-118. doi: 10.1016/j.colsurfb.2015.09.006 [23] CHEN Z, CHENG L, HE Y, et al. Extraction, characterization, utilization as wound dressing and drug delivery of Bletilla striata polysaccharide: A review[J]. Int J Biol Macromol, 2018, 120: 2076-2085. doi: 10.1016/j.ijbiomac.2018.09.028 [24] KING B, BARRETT S, CUTTING KF. Clinical evaluation of a bioactive beta-glucan gel in the treatment of "hard-to-heal" wounds[J]. J Wound Care, 2017, 26(2): 58-63. doi: 10.12968/jowc.2017.26.2.58 [25] TANSAZ S, BOCCACCINI AR. Biomedical applications of soy protein: A brief overview[J]. J Biomed Mater Res A, 2016, 104(2): 553-569. doi: 10.1002/jbm.a.35569 [26] YANG FM, QIN XM, ZHANG T, et al. Effect of oral administration of active peptides of Pinctada martensii on the repair of skin wounds[J]. Mar Drugs, 2019, 17(12): 697. doi: 10.3390/md17120697 [27] 游洋, 包海鹰. 不同成熟期大秃马勃子实体提取物的抑菌活性及其挥发油成分分析[J]. 菌物学报, 2011, 30(3): 477-485. https://www.cnki.com.cn/Article/CJFDTOTAL-JWXT201103018.htm [28] 汤陆扬, 卢小鸾, 杨敏, 等. 雪松醇的合成应用及其药理作用研究进展[J]. 化学试剂, 2021, 43(1): 48-54. https://www.cnki.com.cn/Article/CJFDTOTAL-HXSJ202101008.htm [29] SAIN S, NAOGHAREP, DEVI S, et al. Beta caryophyllene and caryophyllene oxide, isolated from Aegle marmelos, as the potent anti-inflammatory agents against lymphoma and neuroblastoma cells[J]. Anti Inflamm Anti Allergy Agents Med Chem, 2014, 13(1): 45-55. http://www.ncbi.nlm.nih.gov/pubmed/24484210?dopt=Abstract: [30] LI XJ, YANG YJ, LI YS, et al. α-Pinene, linalool, and 1-octanol contribute to the topical anti-inflammatory and analgesic activities of frankincense by inhibiting COX-2[J]. J Ethnopharmacol, 2016, 179: 22-26. doi: 10.1016/j.jep.2015.12.039 [31] 杨华, 徐风, 万丹, 等. 甲基丁香酚镇痛抗炎作用及机制研究[J]. 中药新药与临床药理, 2017, 28(3): 292-297. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYXY201703007.htm [32] 刘晓宇, 陈旭冰, 陈光勇. β-石竹烯及其衍生物的生物活性与合成研究进展[J]. 林产化学与工业, 2012, 32(1): 104-110. https://www.cnki.com.cn/Article/CJFDTOTAL-LCHX201201023.htm [33] 岳丽丹, 杜莉杰, 杨佳宁, 等. ICP-MS法测定及PCA分析不同品种马勃无机元素含量研究[J]. 亚太传统医药, 2021, 17(4): 41-46. https://www.cnki.com.cn/Article/CJFDTOTAL-YTCT202104014.htm [34] 姚波, 刘万宏, 傅亚. 影响创面愈合的营养因素研究进展[J]. 基因组学与应用生物学, 2012, 31(6): 640-643. https://www.cnki.com.cn/Article/CJFDTOTAL-GXNB201206022.htm [35] 王雪芹, 孙隆儒. 中药脱皮马勃的化学成分研究[J]. 天然产物研究与开发, 2007, 19(5): 809-810, 846. doi: 10.3969/j.issn.1001-6880.2007.05.018 [36] 苏明智, 罗舟, 颜鸣, 等. 脱皮马勃化学成分的研究[J]. 中草药, 2012, 43(4): 664-666. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO201204011.htm [37] 崔磊, 宋淑亮, 孙隆儒. 脱皮马勃化学成分研究及抗肿瘤活性的初筛[J]. 中药材, 2006, 29(7): 703-705. doi: 10.3321/j.issn:1001-4454.2006.07.027 [38] 高云佳, 赵庆春, 闵鹏, 等. 脱皮马勃化学成分的研究[J]. 中国药物化学杂志, 2010, 20(1): 47-49. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYH201001013.htm [39] 王利伟, 吴霜. 脱皮马勃化学成分研究[J]. 齐齐哈尔大学学报(自然科学版), 2017, 33(4): 57-58, 61. doi: 10.3969/j.issn.1007-984X.2017.04.013 [40] 金向群, 王隶书, 程东岩, 等. 大马勃的化学成分研究[J]. 中草药, 1998, 29: 298-300. doi: 10.3321/j.issn:0253-2670.1998.05.004 [41] 相聪坤, 关胜江, 王巧, 等. 不同提取方法马勃中药材挥发性成分的GC-MS分析[J]. 河北医药, 2016, 38(23): 3545-3547. https://www.cnki.com.cn/Article/CJFDTOTAL-HBYZ201623006.htm [42] 游洋, 包海鹰. 不同成熟期大秃马勃子实体提取物的抑菌活性及其挥发油成分分析[J]. 菌物学报, 2011, 30(3): 477-485. https://www.cnki.com.cn/Article/CJFDTOTAL-JWXT201103018.htm [43] 徐淑楠, 司攀, 高玉琼, 等. 脱皮马勃挥发性成分的GC-MS分析[J]. 中国实验方剂学杂志, 2012, 18(16): 132-134. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX201216040.htm [44] 阙华发, 张臻, 邢捷, 等. 中医药促进慢性难愈性创面愈合的机制研究[C]. 上海: 中华中医药学会周围血管病分会2010年学术大会论文集, 2010: 77-81. [45] DOWLING MB, SMITH W, BALOGH P, et al. Hydrophobically-modified chitosan foam: Description and hemostatic efficacy[J]. J Surg Res, 2015, 193(1): 316-323. doi: 10.1016/j.jss.2014.06.019 [46] 杨佳宁, 张振凌, 陈晶晶, 等. 脱皮马勃不同部位止血作用及吸附力的比较研究[J]. 中华中医药学刊, 2020, 38(8): 157-161. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYHS202008038.htm [47] 张帆, 王大可, 李爱欣, 等. 大马勃抗炎镇痛及体外抑菌作用的研究[J]. 中国食用菌, 2014, 33(2): 50-52. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSYJ201402021.htm [48] 安成, 周育平, 李春生. 复方马勃冲剂对肺炎球菌感染家兔TNF-α、IL-6的影响[J]. 医学理论与实践, 2001, 14(8): 708-709. doi: 10.3969/j.issn.1001-7585.2001.08.004 [49] MACLEOD AS. Bad staph in the wound environment of diabetic foot ulcers[J]. Cell Host Microbe, 2019, 25(5): 638-640. doi: 10.1016/j.chom.2019.04.006 [50] BILGEN F, URAL A, KURUTASEB, et al. The effect of oxidative stress and Raftlin levels on wound healing[J]. Int Wound J, 2019, 16(5): 1178-1184. doi: 10.1111/iwj.13177 [51] 李作美, 柯春林, 王永斌. 大秃马勃液体发酵菌丝粗多糖的肝损伤保护作用及体外抗氧化活性[J]. 食用菌学报, 2015, 22(4): 70-74. https://www.cnki.com.cn/Article/CJFDTOTAL-SYJB201504018.htm [52] Effect of intensive glucose control on microvascular events in people with type 2 diabetes[J]. Drug Ther Bull, 2017, 55(6): 61-62. [53] OGBOLE OO, NKUMAH AO, LINUS AU, et al. Molecular identification, in vivo and in vitro activities of Calvatia gigantea (macro-fungus) as an antidiabetic agent[J]. Mycology, 2019, 10(3): 166-173. doi: 10.1080/21501203.2019.1595204 [54] PANG LX, WANG YP, ZHENG MQ, et al. Transcriptomic study of high-glucose effects on human skin fibroblast cells[J]. Mol Med Rep, 2016, 13(3): 2627-2634. doi: 10.3892/mmr.2016.4822 [55] 瞿鹏. 马勃油纱促进感染创面愈合作用的实验研究[D]. 哈尔滨: 黑龙江中医药大学, 2013. [56] 石毅, 马莹慧, 门丽慧, 等. 中药马勃促大鼠成纤维细胞增殖的有效成分[J]. 中国老年学杂志, 2012, 32(13): 2762-2764. doi: 10.3969/j.issn.1005-9202.2012.13.042 [57] 程飚, 付小兵. 微环境控制是实现创面完美修复的必由之路[J]. 中华烧伤杂志, 2020, 36(11): 1003-1008. doi: 10.3760/cma.j.cn501120-20201009-00429 [58] 王晓雨, 张玉杰, 曲春安, 等. 成纤维细胞在糖尿病患者创伤愈合中作用的研究进展[J]. 中国细胞生物学学报, 2019, 41(4): 710-714. https://www.cnki.com.cn/Article/CJFDTOTAL-XBZZ201904023.htm [59] 戴小青, 王丽萍, 李华, 等. 马勃联合鱼肝油滴剂治疗压疮的疗效观察及护理体会[J]. 基层医学论坛, 2016, 20(1): 34-35. https://www.cnki.com.cn/Article/CJFDTOTAL-YXLT201601020.htm [60] 孙玲. 马勃油膏治疗褥疮[J]. 医药产业资讯, 2006, 3(14): 320. https://www.cnki.com.cn/Article/CJFDTOTAL-YYCY200614257.htm [61] 董鹏, 张丽兰. 复方马勃煎剂治疗臁疮28例[J]. 中国社区医师, 2002, 18(15): 41. https://www.cnki.com.cn/Article/CJFDTOTAL-XCYS200215053.htm [62] 仲济民, 陈爱东. 马勃纱布治疗冻疮[J]. 中成药研究, 1982, 4: 42. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYA198212030.htm [63] 詹跃燕, 冯锦, 唐菊玲. 马勃粉外用治疗老年性外阴湿疹的疗效观察[J]. 中国中医药科技, 2015, 22(3): 294. https://www.cnki.com.cn/Article/CJFDTOTAL-TJYY201503030.htm [64] 赵志新. 马勃明胶海绵治疗干槽症112例临床观察[J]. 河北中医, 2011, 33(12): 1809-1810. doi: 10.3969/j.issn.1002-2619.2011.12.024 [65] 朱朝军, 张朝晖, 马静, 等. 干性与湿性愈合疗法在糖尿病足坏疽治疗中的应用[J]. 中华损伤与修复杂志(电子版), 2014, 9(2): 186-188. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHSX201402017.htm [66] HU GY, SONG B, JIANG AR, et al. Multifunctional silicon-carbon nanohybrids simultaneously featuring bright fluorescence, high antibacterial and wound healing activity[J]. Small Weinheim Der Bergstrasse Ger, 2019, 15(9): e1803200. doi: 10.1002/smll.201803200 [67] SHANG MG, ZHANG J, LIU XC, et al. N, S self-doped hollow-sphere porous carbon derived from puffball spores for high performance supercapacitors[J]. Appl Surf Sci, 2021, 542: 148697. doi: 10.1016/j.apsusc.2020.148697