植物三萜类成分生物合成中氧鲨烯环化酶与细胞色素P450的研究进展

田荣; 谷巍; 韦陈彬; 徐飞; 邱蓉丽; 张阿琴; 季媛媛; 李桃

doi:10.14148/j.issn.1672-0482.2021.0303

植物三萜类成分生物合成中氧鲨烯环化酶与细胞色素P450的研究进展

doi: 10.14148/j.issn.1672-0482.2021.0303

田荣^1,,
谷巍^{1, 2, ,},
韦陈彬¹,
徐飞¹,
邱蓉丽¹,
张阿琴¹,
季媛媛¹,
李桃¹

1.
南京中医药大学药学院，江苏南京 210023
2.
江苏省中药资源产业化过程协同创新中心, 江苏南京 210023

基金项目:

国家自然科学基金 82073958

国家自然科学基金 81673534

中医药公共卫生服务补助专项财社〔2018〕43号

医疗服务与保障能力提升补助资金(中医药事业传承与发展部分) 财社〔2019〕39号

江苏省中药资源产业化过程协同创新中心项目 ZDXM-3-24

详细信息

作者简介:
田荣，女，博士研究生，E-mail: tianrong0128@163.com

通讯作者:
谷巍，女，教授，主要从事中药资源生产与合成生物学研究，E-mail: guwei9926@126.com

中图分类号: R282.71
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- 被引次数: 0
出版历程
- 收稿日期: 2020-11-26
- 网络出版日期: 2021-12-21
- 刊出日期: 2021-03-10
- 发布日期: 2021-03-15

Advances in Research on the Oxidosqualene Cyclases and Cytochrome P450 Involved in Plant Triterpene Biosynthesis

1.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, 210023, China

摘要

摘要: 三萜是许多药用植物的活性成分，由于结构相对复杂，植物中含量较低，通过提取分离或化学合成难以获得，其应用受到限制。目前对三萜类成分的生物合成研究已取得一定的进展，其中氧鲨烯环化酶(OSC)可催化2, 3-氧化鲨烯环化生成三萜类前体物质，细胞色素P450(CYP450)主要参与三萜的后修饰过程，对三萜的多样性起着关键作用。通过OSC和CYP450在三萜生物合成中的催化功能的综述，以期为进一步探索OSC和CYP450在三萜生物合成中的功能研究提供思路。
- 三萜类成分 /
- 氧鲨烯环化酶 /
- 细胞色素P450
Abstract: Triterpenes, the active ingredients of many medicinal plants, are difficult to be extracted, separated or chemical synthesized because of their complex structures and low amount in plants. Thus, the application of triterpenes is extensively limited. Until now, progress on the triterpene biosynthesis pathway has been made to some progress. Notably, oxidosqualene cyclase (OSC) can catalyze 2, 3-oxidosqualene cyclization to generate precursors of triterpene. And cytochrome P450 (CYP450) is mainly involved in the post-modification process, playing a key role in the diversity of triterpenes. The aim of present study is to provide ideas for the functional research of key enzymes in triterpene biosynthesis by reviewing the researches about the catalytic function of OSC and CYP450 in triterpene biosynthesis.
- triterpenes /
- oxidosqualene cyclase /
- CYP450

HTML全文

图 1 三萜生物合成路径图

注：AACT.乙酰辅酶A酰基转移酶(Acetyl-CoA acetyltrans-ferase)；HMGS.羟甲基戊二酰CoA合酶(3-Hydroxy-3-methylglutaryl-CoA synthase)；HMGR.羟甲基戊二酰CoA还原酶(Hydroxymethyl-glutaryl-CoA reductase)；MK.甲羟戊酸激酶(Meval-onate kinase)；PMK.二氧磷基MVA激酶(Phosphomevalonate kinase)；MVD.甲羟戊酸-5-焦磷酸脱羧酶(Mevalonate-5-pyrophosphate decarboxylase)；IDI.异戊烯基二磷酸异构酶(Lisopentenyl diphosphate isomerase)；FPPS.法呢基焦磷酸合酶(Farnesyl pyrophosphate synthase)；SS.鲨烯合成酶(Squalene synthetase)；SE.鲨烯环氧酶(Squalene epoxidase)；OSC.氧鲨烯环化酶(Oxidosqualene cyclases)；LS.羊毛甾醇合成酶(Lanosterol synthase)；DS.达玛烯合成酶(Dammarenediol synthase)；CS.葫芦二烯醇合酶(Cucurbitadienol synthase)；CAS.环阿屯醇合成酶(Cycloartenol synthase)；PS.帕克醇合酶(Parkeol synthase)；OS.籼稻醇合酶(Orysatinol synthase)；β-AS.β-香树素合成酶(β-Amyrin synthase)；LUS.羽扇醇合成酶(Lupeol synthase)；α-AS.α-香树脂醇合成酶(α-Amyrin synthase)；FS.木栓酮合成酶(Friedelane synthase)

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