六灵解毒丸对急性咽炎的治疗作用研究

高雨嫣; 束安梅; 朱逸晖; 姚瑶; 龚剑; 吕高虹; 王恒斌; 罗年翠; 许惠琴

六灵解毒丸对急性咽炎的治疗作用研究

高雨嫣^1,2,
束安梅^1,2,
朱逸晖^1,2,
姚瑶^1,2,
龚剑¹,
吕高虹^1,2,
王恒斌³,
罗年翠³,
许惠琴^1,2

1.
南京中医药大学药学院，江苏南京 210023
2.
江苏省中药药效与安全性评价重点实验室，江苏南京 210023
3.
雷允上药业集团有限公司，江苏苏州 215009

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出版历程
- 刊出日期: 2020-11-10

Study on the Therapeutic Effect of Liuling Jiedu Wan on Acute Pharyngitis

1.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
Jiangsu Key Lab for Pharmacy and Safety Evaluation of Chinese Materia Medicine, Nanjing, 210023, China
3.
LEI YUN SHANG Pharmaceutical Group Co. Ltd., Suzhou, 215009, China

摘要

摘要: 目的研究六灵解毒丸对急性咽炎的治疗作用。方法采用向幼龄大鼠咽部喷雾15%氨水的方法制备大鼠急性咽炎模型。将造模成功的大鼠随机分为5组，即模型组、蓝芩口服液组、六灵解毒丸3个剂量组（4.375、8.75、17.5 mg/kg），每天灌胃给药1次。空白组与模型组大鼠每天用生理盐水灌胃1次。各组均连续灌胃7 d。实验期间观察大鼠体质量、咽喉红肿等体征。给药结束后，用HE染色法观察大鼠咽部病变情况，取血清和咽黏膜下的结缔组织检测炎症因子的表达情况。采用小鼠毛细血管通透性与耳肿胀实验研究六灵解毒丸的抗炎作用。结果与空白组相比，模型组大鼠时常抓挠口咽部且体质量增长缓慢，咽部明显红肿，组织结构异常，有大量结缔组织增生和炎症细胞浸润。给予大鼠六灵解毒丸后，大鼠体质量增长加快，咽部红肿程度下降，组织结构有明显改善，黏膜下层腺体结构正常，未见结缔组织增生与炎症细胞浸润。六灵解毒丸能明显下调大鼠血清与组织中的IL-1β、IL-6、TNF-α水平（P<0.05~0.01）。另外，六灵解毒丸对二甲苯所致小鼠耳肿胀和乙酸所致的小鼠腹腔毛细血管通透性升高均有显著的抑制作用（P<0.05~0.01），其中六灵解毒丸中、高剂量组抑制耳肿胀作用明显优于蓝芩口服液。结论六灵解毒丸对于急性咽炎具有良好的治疗作用，其机制可能与下调IL-1β、IL-6、TNF-α水平相关。在临床等效剂量下，六灵解毒丸的作用略优于蓝芩口服液，表明六灵解毒丸有较好的治疗急性咽炎的作用。
- 六灵解毒丸 /
- 急性咽炎 /
- IL-1β /
- IL-6 /
- TNF-α
Abstract: OBJECTIVE To study the therapeutic effect of Liuling Jiedu Wan on acute pharyngitis. METHODS The model of acute pharyngitis was prepared by spraying 15% ammonia water into the young rats' pharynx. Rats with successful modeling were randomly divided into 5 groups， namely model group， Lanqin Oral Liquid group and Liuling Jiedu Wan group with 3 doses. Rats in the control and model groups were given normal saline by gavage. The drugs were given once a day by gavage continuously for 7 days. During the course of gavage， body weight， laryngeal redness and other signs of the rats were observed. After administration， HE staining was used to observe the pharyngeal lesions in rats， and the expression of inflammatory factors in the serum and the connective tissue under the pharyngeal mucosa were detected. The anti-inflammatory effect of Liuling Jiedu Wan was studied by capillary permeability and ear swelling in mice. RESULTS Compared with the control group， rats in the model group frequently scratched the oropharynx and experienced slow weight gain， obvious pharyngeal redness and swelling. Their tissue structures were abnormal， with a large number of connective tissue hyperplasia and inflammatory cell infiltration. After the administration of Liuling Jiedu Wan， the weights of the rats increased rapidly， the degrees of pharyngeal redness and swelling decreased， and the tissue structures were significantly improved. The structures of submucosal glands were normal， and no connective tissue hyperplasia and inflammatory cell infiltration were observed. Moreover， Liuling Jiedu Wan could significantly decrease the levels of IL-1β， IL-6 and TNF-α in serum and tissues of rats (P<0.05，P<0.01). Liuling Jiedu Wan could significantly reduce the capillary permeability caused by acetic acid and reduce the degree of ear swelling caused by xylene (P<0.05，P<0.01). The effects of Liuling Jiedu Wan on ear swelling in the middle and high dose groups were significantly better than that of Lanqin Oral Liquid. CONCLUSION Liuling Jiedu Wan has a good therapeutic effect on acute pharyngitis， and its mechanism may be related to the down-regulation of IL-1β， IL-6 and TNF-α levels. Under the clinical equivalent dose， the anti-inflammatory effect of Liuling Jiedu Wan is slightly better than that of Lanqin Oral Liquid， indicating that Liuling Jiedu Wan has a better effect on acute pharyngitis.
- Liuling Jiedu Wan /
- acute pharyngitis /
- IL-1β /
- IL-6 /
- TNF-α

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参考文献(16)

[1]	急喉痹的诊断依据、证候分类、疗效评定——中华人民共和国中医药行业标准《中医内科病证诊断疗效标准》（ZY/T001.1-94）[J].辽宁中医药大学学报， 2019， 21(12): 193.
[2]	BARBIERI E， DONA D， CANTARUTTI A， et al. Antibiotic prescriptions in acute otitis media and pharyngitis in Italian pediatric outpatients[J]. Ital J Pediatr， 2019， 45(1): 103.
[3]	TANZ RR， GEWITZ MH， KAPLAN EL， et al. Stay the course: Targeted evaluation， accurate diagnosis， and treatment of streptococcal pharyngitis prevent cute rheumatic fever[J]. J Pediatr， 2020， 216: 208-212.
[4]	王丽华，李文华，沙一飞，等. 疏风解毒胶囊联合天突穴穴位注射疗法治疗风热型急性咽炎180例临床研究[J]. 中华中医药杂志， 2017， 32(1): 376-379.
[5]	国家药典委员会. 中华人民共和国药典:一部[S]. 北京: 中国医药科技出版社， 2015.
[6]	BAOSONG L， MING B， MENGFAN P， et al. Anti-inflammatory effect and the effect on acute pharyngitis rats model of compound Lobelia oral liquid[J]. Saudi J Biol Sci， 2019， 26(3): 577-581.
[7]	陈会丛，杨海润，杜莹洁，等. 同仁堂儿童清肺口服液解热、抗炎、抑菌作用研究[J]. 中华中医药杂志， 2016， 31(10): 4291-4294.
[8]	樊江波，黄琳红. 益母草活性成分芫花素对小鼠凝血及抗炎作用的研究[J]. 西安交通大学学报(医学版)， 2019， 40(1): 158-161.
[9]	李喜平，张程亮，刘东. 牛黄的现代研究(四):药理作用[J]. 医药导报， 2017， 36(4): 355-360.
[10]	TSE CF， CHAN YYF， POON KM， et al. Clinical prediction rule to predict pneumonia in adult presented with acute febrile respiratory illness[J]. Am J Emerg Med， 2019， 37(8):1433-1438.
[11]	LI X， BIAN Y， PANG P， et al. Inhibition of Dectin-1 in mice ameliorates cardiac remodeling by suppressing NF-κB/NLRP3 signaling after myocardial infarction[J]. Int Immunopharmacol， 2020， 80: 106116.
[12]	陈霞，杨磊，王丽，等. 血清呼吸道解脲支原体感染诱发呼吸窘迫综合征患儿血清肿瘤坏死因子-α、白介素-1β、白介素-6水平变化及其临床意义[J]. 中国医学前沿杂志(电子版)， 2020， 12(3): 116-119.
[13]	文德鉴，袁丽君，李三宇，等. 复方扁咽宁对急性咽炎大鼠疗效及其机制[J]. 中国应用生理学杂志， 2019， 35(1): 19-22， 27.
[14]	马建丽，黎春彤，李翔，等. 扁咽口服液对急性咽炎大鼠咽喉组织基质金属蛋白-9及血清IL-1β和TNF-α表达的影响[J]. 解放军药学学报， 2017， 33(2): 138-141.
[15]	苗明三，常兵杰，白明，等. 急性咽炎动物模型制备规范(草案)[J]. 中药药理与临床， 2018， 34(1): 175-178.
[16]	邹秦文，石岩，魏锋，等. 牛黄系列药材化学成分比较及其药理作用研究概况[J]. 中国药事， 2014， 28(6): 646-650.