半夏厚朴汤对顺铂所致水貂呕吐模型的作用及机制研究

黄仕文; 范方田; 嵇晶; 谭峰; 程建明; 喻斌; 牛迪

半夏厚朴汤对顺铂所致水貂呕吐模型的作用及机制研究

1.
南京中医药大学中医学院·中西医结合学院，江苏南京 210023
2.
蚌埠医学院药学院，安徽蚌埠 233030
3.
南京中医药大学药学院，江苏南京 210023

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出版历程
- 刊出日期: 2020-11-10

Effect and Mechanism of Banxia Houpu Tang on Mink Vomiting Model Induced by Cisplatin

1.
School of Chinese Medicine, School of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
School of Pharmacy, Bengbu Medical College, Bengbu, 233030, China
3.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China

摘要

摘要: 目的探讨半夏厚朴汤（BXHPT）对顺铂所致水貂呕吐模型的治疗作用以及对P物质（SP）/NK1R信号的影响。方法 36只水貂随机分为正常对照组，模型组，昂丹司琼组，BXHPT低、中、高剂量组（200、400、800 mg/kg），各组分别给予相应的药物连续7 d，后采用顺铂腹腔注射构建呕吐模型，测定水貂的干呕和呕吐次数，ELISA法检测水貂大脑最后区SP的含量，Western blot法测定大脑最后区NK1R、p-ERK1/2、ERK1/2的蛋白水平。结果模型组水貂干呕、呕吐次数，大脑最后区SP水平，NK1R，p-ERK1/2蛋白水平均明显高于正常对照组（P<0.05）。而BXHPT组水貂干呕、呕吐次数，大脑最后区SP水平，NK1R，p-ERK1/2蛋白水平均明显低于模型组（P<0.05）。结论 BXHPT可以治疗顺铂导致的水貂的呕吐，其机制可能与降低水貂大脑最后区SP/NK1R信号活化有关。
- 呕吐 /
- 顺铂 /
- 半夏厚朴汤 /
- P物质 /
- NK1R
Abstract: OBJECTIVE To investigate the effect of Banxia Houpu Tang (BXHPT) on the model of mink vomiting induced by cisplatin and substance P/NK1R pathway. METHODS 36 minks were randomly divided into normal control group， model group， ondansetron group， BXHPT low， medium and high dose group (200， 400， 800 mg/kg). Each group was given corresponding drugs， respectively， for seven days. Then intraperitoneal injection of cisplatin was used to construct vomit model， the times of retching and vomit were measured， the content of substance P in the last area of mink brain were measured by ELISA， the protein levels of NK1R， p-ERK1/2 and ERK1/2 in the last area of the brain were measured by Western blot. RESULTS The levels of retching， vomit times， substance P， NK1R and p-ERK1/2 protein in the last area of brain in the model group were significantly higher than those in the normal control group (P<0.05). In BXHPT group， the retch， vomit times， substance P level， NK1R and p-ERK1/2 protein levels were significantly lower than those in model group (P<0.05). CONCLUSION BXHPT can treat the vomiting of mink caused by cisplatin， and the mechanism may be related to the decrease of substance P/NK1R signal activation in the last brain area of minks.
- vomiting /
- cisplatin /
- Banxia Houpu Tang /
- substance P /
- NK1R

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参考文献(10)

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