灵芝多糖的抗氧化作用研究
Study on Antioxidation of Ganoderma Lucidum Polysaccharide
-
摘要: 目的 研究灵芝多糖的抗氧化作用,为灵芝多糖化妆品抗皮肤衰老功效提供科学依据。方法 化学方法评价灵芝多糖的总还原能力、羟基自由基和DPPH自由基清除能力;检测灵芝多糖脂质体过氧化进程中过氧化物和丙二醛(MDA)的产量考察其抗脂质体氧化能力;体外细胞实验考察灵芝多糖对H2O2诱导氧化损伤的HaCaT细胞的保护作用;在体实验考察自制灵芝多糖乳液对D-半乳糖亚急性小鼠衰老模型皮肤中超氧化物歧化酶(SOD)活力、MDA和羟脯氨酸(Hyp)含量的影响。结果 灵芝多糖的还原能力和清除2种自由基能力均呈浓度依赖性,而且它能抑制脂质过氧化物生成,60 h灵芝多糖脂质体产生的过氧化物为空白脂质体的九分之一,MDA产量为空白脂质体的三分之一。灵芝多糖在0.312 5~1.25 mg/mL的范围内对H2O2氧化损伤的HaCaT细胞有保护作用(P<0.01),且保护作用随多糖浓度升高而增强。小鼠皮肤涂抹低剂量自制灵芝多糖乳液后,皮肤中SOD活力显著高于模型组(P<0.05);高剂量涂抹时,MDA含量显著下降,Hyp含量显著升高(P<0.05),自制灵芝多糖乳液抗氧化能力略优于市售乳液。结论 多层面评价了灵芝多糖的抗氧化活性,初步研制出具有抗衰老功效的灵芝多糖乳液,为灵芝多糖抗衰老化妆品的开发提供研究基础。Abstract: OBJECTIVE To study the antioxidant activity of Ganoderma lucidum polysaccharides(GLP), and to provide scientific basis for its application of anti-aging cosmetics. METHODS Chemical method was used to evaluate the total reducing ability, hydroxy radical and DPPH free radical scavenging ability of GLP. The anti-lipid peroxidation ability of GLP was investigated with respect to the production of peroxid and malondialdehyde. The effects of GLP on the protective effect of H2O2-induced oxidative damage on HaCaT cells was investigated by in vitro cell experiments. The effects of lab-made GLP emulsion on superoxide dismutase (SOD) activity, malondialdehyde (MDA), and hydroxyproline (Hyp) contents in the skin of D-galactose subacute mouse aging model were investigated in vivo. RESULTS The reducing ability and the ability to scavenge two radicals above were concentration-dependent. At the same time, it showed that GLP could inhibit the production of lipid peroxidation products. At 60 h, the peroxide produced by GLP liposomes were one-ninth of that of blank liposomes, and the production of malondialdehyde were one-third of it. Furthermore, GLP had a protective effect on H2O2 induced oxidatively damaged HaCaT cells (P<0.01), and the protective effect was enhanced with increasing polysaccharide concentration. The results of animal experiments showed that the SOD activity in the skin was significantly higher than that in the model group (P<0.05) after applying low-dose homemade GLP emulsion to the skin of mice; when applied at high doses, the MDA content decreased significantly and the Hyp content increased significantly (P<0.05), the antioxidant capacity of lab-made GLP emulsion is slightly better than the commercially available emulsion. CONCLUSION The antioxidative activity of GLP was evaluated at multiple levels, and GLP emulsions with anti-aging effects were initially prepared, which provided a research basis for the development of GLP anti-aging cosmetics.
-
Key words:
- Ganoderma lucidum polysaccharide /
- antioxidant /
- liposomes /
- HaCaT cell /
- skin aging
-
[1] OLIVEIRA MM, RATTI BA, DARE RG, et al.Dihydrocaffeic acid prevents UVB-induced oxidative stress leading to the inhibition of apoptosis and MMP-1 expression via p38 signaling pathway[J]. Oxid Med Cell Longev, 2019,2019:2419096. [2] 丁秋英.金福菇多糖抗衰老作用研究[D].合肥:安徽大学,2016. [3] ZHEN AX,PIAO MJ,KANG KA,et al.Esculetin prevents the induction of matrix metalloproteinase-1 by hydrogen peroxide in skin keratinocytes[J].J Cancer Prev,2019,24(2):123-128. [4] 才晓玲,何伟,杨国琴.灵芝多糖生物活性研究进展[J].食用菌,2018,40(3):1-4. [5] 王颖,魏佳韵,吴思佳,等.灵芝多糖结构特征及药理作用的研究进展[J].中成药,2019,41(3):627-635. [6] 王波.灵芝多糖提取、分离纯化、表征及体外抗氧化活性探究[D].郑州:郑州大学,2016. [7] 陈杰.不同产地灵芝子实体化学成分及抗氧化活性研究[D].天津:天津科技大学,2016. [8] 王新.灵芝多糖的提取及抗氧化活性研究[D].镇江:江苏大学,2007. [9] 周鸿立,张硕,胡乐安,等.玉米须粗多糖体外抗氧化活性研究[J].食品研究与开发,2015,36(18):11-14. [10] 王红丽,吴铁,钱聚标,等.D-半乳糖致衰老模型小鼠皮肤衰老的观察[J].中华老年医学杂志,2004(8):45-47. [11] 黄玲玲.一种芳香类中药复方成分分析及抗皮肤衰老功效研究[D].福州:福建农林大学,2012. [12] 陈琳琳,谢志新,程晶,等.羊栖菜多糖提取物及其润肤霜的保湿和抗氧化活性[J].日用化学工业,2018,48(1):31-36. [13] 李诚桐,赵华.化妆品功效评价(Ⅳ):延缓皮肤衰老功效宣称的科学支持[J].日用化学工业,2018(4):188-195. [14] 王晓宇,杜国荣,李华.抗氧化能力的体外测定方法研究进展[J].食品与生物技术学报,2012,31(3):247-252. [15] 曹玉娜,宋志前,魏征,等.抗氧化剂的抗氧化活性测定方法研究进展[J].中国药房,2013,24(1):86-88. [16] 张文强.低分子量透明质酸脂质体的制备及体外经皮渗透研究[D].广州:华南理工大学,2010. [17] 耿亚男.磷脂氧化对脂质体性质的影响[D].无锡:江南大学,2013. [18] 吴梦迪.铁卟啉催化活性及其应用研究[D].西安:西北大学,2017. [19] 黄娜娜.柑橘精油抗氧化特性及对皮肤细胞氧化损伤的保护作用研究[D].武汉:华中农业大学,2016.
点击查看大图
计量
- 文章访问数: 814
- HTML全文浏览量: 45
- PDF下载量: 753
- 被引次数: 0