越鞠甘麦大枣汤对产后抑郁小鼠前额叶BDNF-TrkB通路的影响

夏宝妹; 陈畅; 唐娟娟; 周欣; 陈刚

越鞠甘麦大枣汤对产后抑郁小鼠前额叶BDNF-TrkB通路的影响

夏宝妹¹,
陈畅²,
唐娟娟³,
周欣⁴,
陈刚⁵

1.
南京特殊教育师范学院康复科学学院，江苏南京 210023
2.
南京中医药大学附属南京中医院，江苏南京 210022
3.
南京中医药大学医学院·整合医学学院，江苏南京 210023
4.
南京中医药大学中医学院·中西医结合学院，江苏南京 210023
5.
暨南大学中医学院脑病个性化防治跨学科研究所，广东广州 510632

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出版历程
- 刊出日期: 2020-03-10

Effects of Yueju Ganmai Dazao Decoction on BDNF-TrkB Pathway in Prefrontal Lobe of Postpartum Depression Mice

1.
Faculty of Rehabilitation Science, Nanjing Normal University of Special Education, Nanjing, 210023, China
2.
Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, China
3.
School of Medicine & Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China
4.
School of Traditional Chinese Medicine & Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China
5.
Interdisciplinary Institute for Personalized Medicine in Brain Disorders, Jinan University, Guangzhou, 510632, China

摘要

摘要: 目的探讨越鞠甘麦大枣汤（越甘汤）对产后抑郁症(Postpartum depression，PPD)小鼠的快速抗抑郁作用，并从脑源性神经营养因子(BDNF)-酪氨酸蛋白激酶B（TrkB）通路探讨PPD的发病机制及中药起效的作用机制。方法将40只Balb/c雌性小鼠随机分为2组，空白组不给予任何刺激，孕前应激组给予慢性束缚刺激。3周后将孕前应激组小鼠进行雌雄合笼交配怀孕，分娩后3周检测小鼠的糖水偏好率和强迫游泳不动时间。将孕前应激组诱导建立的PPD小鼠随机分为3组：模型组、越甘汤组和氯胺酮组，检测药物对小鼠的干预作用，并取小鼠前额叶检测BDNF、TrkB、糖原合酶激酶3β（GSK3β）和p-GSK3β蛋白的表达。结果产后3周，与空白组比较，模型组的糖水偏好率显著降低（P<0．01），强迫游泳不动时间显著延长（P<0．01），前额叶BDNF、TrkB及p-GSK3β/GSK3β的表达均显著下调（P<0．05）。单次给予越甘汤或氯胺酮24 h之后，小鼠的糖水偏好率显著升高（P<0．01），强迫游泳不动时间显著减少（P<0．01），前额叶BDNF、TrkB及p-GSK3β/GSK3β的蛋白表达显著上调(P<0.05）。结论慢性孕前应激诱导Balb/c小鼠表现出产后抑郁样症状，其发病机制可能与激活其前额叶BDNF-TrkB通路有关。越甘汤对PPD模型小鼠的快速抗抑郁作用可能与改善前额叶BDNF-TrkB信号通路有关。
- 产后抑郁 /
- 越鞠甘麦大枣汤 /
- BDNF /
- TrkB /
- GSK3β
Abstract: OBJECTIVE To study the rapid antidepressant effect of Yueju Ganmai Dazao Decoction (Yuegan Decoction， YG) on postpartum depression (PPD) mice， and to explore the pathogenesis of PPD and the mechanism of Chinese medicine from BDNF-TrkB pathway. METHODS 40 Balb/c female mice were randomly divided into two groups. The control group was not given any stimulation. The pre-pregnancy stressed group was subjected to chronic restraint stress. After 3 weeks， the mice in the pre-pregnancy stress group were mated. The mice were arranged for sucrose preference test (SPT) and forced swim test（FST） 3 weeks after delivery. The PPD mice induced by the pre-pregnancy stress were randomly divided into three groups: model group， YG group and ketamine group. The intervention effect of YG on the mice was detected， and the prefrontal lobes of the mice were taken to detect the expression of BDNF， TrkB， p-GSK3β and GSK3β protein. RESULTS After 3 weeks postpartum， the model mice showed depression-like behaviors. Reduced preference in drinking sucrose solution were found in SPT (P<0.01)， and immobility in FST was significantly increased (P<0.01). And the expressions of BDNF， TrkB and p-GSK3β/GSK3β in the prefrontal lobe of the PPD model group were significantly reduced (P<0.05)， compared to control group. Acute YG improved performance in the SPT and FST (P<0.01)， which was similar to ketamine. A single dose of YG or ketamine normalized BDNF-TrkB signaling in the PPD model mice (P<0.05). CONCLUSION Chronic pre-pregnancy stress induces postpartum depression-like symptoms in Balb/c mice. The pathogenesis may be related to activation of the prefrontal BDNF-TrkB pathway. The rapid antidepressant effect of YG on PPD model mice may be related to the improvement of the prefrontal BDNF-TrkB signaling pathway.
- postpartum depression /
- Yueju Ganmai Dazao Decoction /
- BDNF /
- TrkB /
- GSK3β

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参考文献(15)

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