GC-MS分析不同舌苔胃癌患者的血清代谢组学差异

徐硕; 向春婕; 朱振华; 吴娟; 高雅楠; 王瑞平; 詹瑧; 张晓春; 张军峰

GC-MS分析不同舌苔胃癌患者的血清代谢组学差异

1.
南京中医药大学医学与生命科学学院，江苏南京 210023
2.
南京中医药大学药学院，江苏南京 210023
3.
南京中医药大学附属医院，江苏南京 210029
4.
扬州市中医院肿瘤科，江苏扬州 225009

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出版历程
- 刊出日期: 2019-03-10

Serum Metabolomic Difference of the Tongue Coatings in the Patients with Gastric Cancer by GC-MS

1.
School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China
3.
Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China
4.
Department of Oncology, Yangzhou Traditional Chinese Medicine Hospital, Yangzhou, 225009, China

摘要

摘要: 目的比较分析不同舌苔的胃癌患者血清代谢组学机制。方法 101例胃癌患者分为薄白苔组（42例）、白厚苔组（9例）、黄薄苔组（28例）和黄厚苔组（22例），22例薄白苔健康体检者作为对照，采用GC-MS技术检测血清代谢组，运用主成分分析、正交偏最小二乘法判别分析、单变量统计分析方法筛选代谢标志物，富集分析预测代谢通路。结果与对照组相比，薄白苔组有涉及31个代谢通路变化的13种代谢标志分子，白厚苔组有涉及26个代谢通路变化的15种代谢标志分子，黄薄苔组有涉及25个代谢通路变化的9种代谢标志分子，黄厚苔组有涉及12个代谢通路变化的8种代谢标志分子，4种舌苔胃癌患者血清均表现为4-氨基丁酸水平升高并涉及酪氨酸代谢通路。结论胃癌患者不同舌苔反映了不同的血清代谢组学差异。
- 胃癌 /
- 舌苔 /
- GC-MS /
- 代谢组学
Abstract: OBJECTIVE To explore the serum metabolomic mechanism of the common tongue coatings (TCs) in the patients with gastric cancer. METHODS The gastric cancer patients (n=101) were divided into four groups: white thin TC group (n=42)， white thick TC group (n=9)， yellow thin TC group (n=28) and yellow thick TC group (n=22). Twenty-two healthy volunteers with white thin TC were recruited as controls. GC-MS was used to detect the serum metabolomic. Multi-factors analysis methods were conducted to screen the metabolic markers of the common TCs in gastric cancer patients， and enrichment analysis was carried out to predict the metabolic pathways. RESULTS Compared with the control group， the white thin TC of gastric cancer patients occupied 13 special metabolic molecules involved in 31 metabolic pathways， the white thick TC of gastric cancer patients held 15 special metabolic molecules involved in 26 metabolic pathways， the yellow thin TC of gastric cancer patients held 9 special metabolic molecules involved in 15 metabolic pathways， and the yellow thick TC of gastric cancer patients had 8 metabolic molecules involved in 12 metabolic pathways. The level of 4-aminobutyric acid elevated in all the gastric cancer patients were observed involving with tyrosine metabolic pathway. CONCLUSION Different TCs reflect characteristic serum metabolomic machanism in gastric cancer patients.
- gastric cancer /
- tongue coating /
- GC-MS /
- metabolomics

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参考文献(12)

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