“藻戟遂芫”与甘草合用对肠组织P糖蛋白转运功能的影响

麻智祥; 丁岩; 俞辰亚代; 樊宏伟; 华永庆

“藻戟遂芫”与甘草合用对肠组织P糖蛋白转运功能的影响

1.
南京医科大学附属南京医院，江苏南京 210006
2.
南京中医药大学江苏省方剂高技术研究重点实验室，江苏南京 210023

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出版历程
- 刊出日期: 2016-07-10

Impact of "Zao-ji-sui-yuan" Combined with Glycyrrhizae on Rat Intestinal P-glycoprotein Transport Function in Vitro

1.
Nanjing Hospital Affiliated to Nanjing Medical University, Nanjing, 210006, China
2.
Jiangsu Key Laboratory of High Technology Research, Nanjing University of Chinese Medicine, Nanjing, 210023, China

摘要

摘要: 目的从离体肠组织P糖蛋白（P-gp）活性的角度，探讨中药“十八反”中“藻戟遂芫”与甘草合用产生禁忌的可能机理。方法以罗丹明123（R123）作为指示剂，采用Ussing-chamber技术观察“藻戟遂芫”与甘草合用对大鼠离体回肠组织P-gp转运活性的影响。结果甘草能明显促进R123的分泌（P<0.01），该作用可被P-gp抑制剂维拉帕米抑制；海藻、甘遂与芫花显著促进R123的分泌（P<0.05）；海藻、大戟、芫花与甘草合用后R123分泌量与甘草组相比均明显下降（P<0.01）；而甘遂与甘草合用后差异不显著。结论甘草能明显诱导P-gp活性，与海藻、大戟、芫花合用后甘草对P-gp活性的诱导作用被拮抗，该作用可能导致药物毒性成分外排减少，肠组织损伤加剧，提示一些有毒中药与甘草合用后，肠组织P-gp活性的改变可能引起减效或增毒的相反效应。
- “十八反” /
- 配伍禁忌 /
- 甘草 /
- P糖蛋白
Abstract: OBJECTIVE The mechanism of Glycyrrhizae's incompatibility with "zao-ji-sui-yuan"(Sargassum， Euphorbia pekinensis， Euphorbia kansui and Daphne genkwa) was explored by assessing intestinal P-glycoprotein (P-gp) activity in vitro. METHODS Ussing-chamber technique was used to determine intestinal secretory function. Rhodamine123 (R123) was used as an indicator of P-gp activity. RESULTS Glycyrrhizae could significantly promote the secretion of R123. In the present of Sargassum， Euphorbia pekinensis and Daphne genkwa， the promotion effect of Glycyrrhizae was significantly suppressed. CONCLUSION P-gp activity can be significantly induced by Glycyrrhizae. The promotion effect of P-gp activity induced by Glycyrrhizae was antagonized by Sargassum， Euphorbia pekinensis and Daphne genkwa， which might lead to the reduction of toxic components pumping out and then aggravated intestinal tissue injury. These results indicated that when Glycyrrhizae combined with some toxic traditional Chinese medicine， change of the intestinal P-gp activity might cause the "contrary effects".
- Eighteen Incompatible Medicament /
- incompatibility /
- Glycyrrhizae /
- P-glycoprotein

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参考文献(18)

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