当归补血微囊促血管新生中JAK2/STAT3通路的作用研究

陈海军; 潘立群

当归补血微囊促血管新生中JAK2/STAT3通路的作用研究

陈海军^1,2,
潘立群^1,

1.
南京中医药大学第一临床医学院，江苏南京 210023
2.
南京中医药大学附属昆山市中医医院，江苏昆山 215600

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出版历程
- 刊出日期: 2016-07-10

Study on Function of JAK2/STAT3 Pathway in Promoting Angiogenesis with Angelica Microcapsules for Replenishing Blood

CHENHai-jun^1,2,
PANLi-qun^1
,

1.
The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
Traditional Chinese Medicine Hospital of Kunshan Affiliated to Nanjing University of Chinese Medicine, Kunshan, 215600, China

摘要

摘要: 目的探讨JAK2/STAT3信号传导通路在当归补血微囊促血管新生中的作用。方法建立人脐静脉血管内皮细胞（HUVEC）模型，分别采用CCK-8和流式细胞术检测HUVEC细胞活力和细胞凋亡情况；采用Western blot检测各组中p-STAT3、VEGF的表达情况；采用荧光定量PCR，检测各组中VEGF基因和miRNA-21的表达情况。结果 20μg/mL当归补血微囊作用于HUVEC 24h，可有效促进HUVEC的增殖，并上调p-STAT3、VEGF及miRNA-21的表达；AG490能有效抑制当归补血微囊对HUVEC的促增殖作用，并减少p-STAT3、VEGF及miRNA-21的表达。结论当归补血微囊促进血管生成的作用确实与JAK2/STAT3信号途径有关。
- 当归补血微囊 /
- 血管新生 /
- JAK2/STAT3通路 /
- VEGF /
- miRNA-21
Abstract: OBJECTIVE To investigate the function of JAK2/STAT3 pathway in promoting angiogenesis with Angelica microcapsules for replenishing blood (Ang-Mc). METHODS Establish the human umbilical vein endothelial cell (HUVEC) model， and then cell counting kit (CCK-8) and flow cytometry were used to test cell viability and cell apoptosis. Western blot was used to detect expression of p-STAT3 and VEGF. RT-PCR was used to detect expression of vascular endothelial growth factor (VEGF) and miRNA-21. RESULTS When Ang-Mc with a concentration of 20μg/mL acted on HUVEC for 24h， it could effectively promote the HUVEC proliferation by up-regulating the expression of p-STAT3， VEGF and miRNA-21. AG490 can effectively inhibit Ang-Mc promoting HUVEC proliferation effect and reduce the expression of p-STAT3， VEGF and miRNA-21. CONCLUSION The effect of Ang-Mc in promoting angiogenesis is related to JAK2/STAT3 signaling pathways.
- Angelica microcapsules for replenishing blood /
- angiogenesis /
- JAK2/STAT3 pathway /
- VEGF /
- miRNA-21

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