乐脉颗粒对心肌缺血的保护作用及其机制
Protective Effects of Lemai Granules on Myocardial Ischemia and Its Mechanisms
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摘要: 目的 探讨乐脉颗粒对大鼠心肌缺血的作用及其机制。方法 48只SD雄性大鼠随机分为正常组、模型组、阳性(稳心颗粒)组、乐脉颗粒高、中、低剂量组(0.84、0.42、0.21 g/kg)。腹腔注射盐酸异丙肾上腺素(ISO)复制大鼠ISO模型,连续灌胃乐脉颗粒7 d后,记录心电图Ⅱ导联ST段,并取血测定血清中CK、LDH、SOD活性及MAD、NO、ET、IL-1β、TNF-α、CRP含量,同时检测血液流变学指标。结果 与模型组比较,乐脉颗粒各组ST段抬高值及CK、LDH活性均降低(P<0.01);乐脉颗粒高、中剂量组SOD的活性及NO含量均有不同程度的升高,而ET、IL-1β、TNF-α、CRP含量均有不同程度降低(P<0.05~0.01),血液流变学各项指标显著改善。结论 乐脉颗粒改善心肌缺血的作用可能与抗氧化应激损伤、调节内皮细胞的功能、抗炎及改善血流变有关。Abstract: OBJECTIVE To observe the effect of Lemai granules on model rats with myocardial ischemia and explore its mechanism. METHODS 48 male SD rats were randomly divided into six groups: normal group, model group, positive control group (Wenxin granules group)and high, middle and low dose group (0.84, 0.42, 0.21 g/kg) of Lemai granules. Myocardial ischemia rats model was established by intraperitoneal injection of isoproterenol (ISO), and then different doses of Lemai granules were administered intragastrically to the myocardial ischemia rats for 7 d. The changes of the ST-segment of Ⅱlead electrocardiogram (ECG) was observed. The activities of CK, LDH and SOD, the content of MDA, NO, ET, IL-1β,TNF-α and CRP in serum were measured, and changes of blood rheology were tested. RESULTS ST-segment and the activities of CK and LDH of Lemai granules groups(0.84 g/kg, 0.42 g/kg, 0.21 g/kg) were lower obviously than that of model group (P<0.01). Compared with the model group, the activities of SOD and NO in Lemai granule groups of the high and middle doses (0.84 g/kg and 0.42 g/kg) increased in various degrees, while the levels of MDA, ET, IL-1β, TNF-α and CRP were decreased (P<0.05~0.01). The blood rheology were improved significantly. CONCLUSION Lemai granules shows promising protective effects on myocardial ischemia and its mechanism may be related to antioxidant action, regulation of endothelial cells, anti-inflammatory effects and blood rheology improvement.
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Key words:
- Lemai granules /
- myocardial ischemia /
- antioxidation /
- endothelial functions /
- anti-inflammation /
- blood rheology
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