生半夏姜制或煎煮对小鼠妊娠及胚胎发育的影响
Different Effects of Rhizoma Pinelliae Processed by Ginger or Decocting on Gestation and Embryonic Development of Mice
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摘要: 目的 研究生半夏姜制或煎煮对小鼠妊娠及胚胎发育的影响,初步探讨半夏减毒的影响因素。方法 采用一般急性毒性实验法及致畸敏感期药物生殖毒性实验方法,分别比较了生半夏粉混悬液、姜半夏粉混悬液,生半夏汤剂对小鼠的急性毒性及对小鼠妊娠与胚胎发育的影响。结果 急毒试验中,生半夏混悬液(36 g/kg)与生半夏汤剂(180 g/kg)给药后,小鼠死亡率分别为50%、22.2%;姜半夏混悬液组(36 g/kg)与正常组无显著差异。生殖毒性试验中,生半夏混悬液6 g/kg剂量母鼠死亡率达30%,孕期增重少,胎仔尾长短,与正常对照组差异显著(P<0.05);生半夏汤剂30 g/kg对母鼠无影响,胎仔身长短于正常对照组(P<0.05);姜半夏混悬液6 g/kg给药后,所有指标与正常对照组无显著差异。结论 生半夏混悬液急性毒性较高且有显著的母体毒性及胚胎毒性;煎煮后其汤剂毒性降低,对母体无毒性但影响胎仔的生长发育;姜半夏混悬液无任何母体或胚胎毒性。Abstract: OBJECTIVE To study the different effects of Rhizoma Pinelliae(RP) processed by ginger or decocting on the gestation and embryonic development of mice, thus investigating the influential factors on abating toxicity of RP. METHODS Four groups were designed in which mice were treated respectively with PR suspension, ginger processed PR (GPPR) suspension, PR decoction and drinking water. Acute toxicity testing and Teratogenic sensitive period reproductive toxicity testing were used to compare the acute toxicity and effects on gestation and embryonic development of mice. RESULTS In acute toxicity testing, the mortality in mice were respectively 50% and 22.2% in PR suspension group and PR decoction group. However, there was not any significant deviation between the control group and GPPR suspension group. In terms of reproductive toxicity testing, the maternal mortality of PR suspension group (6 g/kg) reached 30%, while gestational maternal weight gain was significantly lower and the tail length (TL) of embryos was significantly shorter than the control group. There were no any maternal effects but the crown-rump length (CRL) of embryos was significantly shorter than the control group after treated with PR decoction (30 g/kg). There were no significant differences between the GPPR suspension Group (6 g/kg) and the control group.CONCLUSION Acute toxicity, maternal toxicity and embryo toxicity were all identified within the PR suspension group while none maternal toxicity was detected within the PR decoction group, however, the growth and development of embryos were affected in the PR decoction group. There was not any maternal toxicity or embryo toxicity in the GPPR suspension group.
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