雷公藤红素对胶原诱导性关节炎小鼠的免疫作用研究
Immune Effects of Tripterine on Collagen Induced Arthritis Model Mice
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摘要: 目的 研究雷公藤红素对牛Ⅱ型胶原诱导的关节炎小鼠抗炎作用及其对T细胞的作用机制。方法 选用DBA/1J小鼠，以牛Ⅱ型胶原诱导建立小鼠关节炎CIA模型。成模后，以雷公藤红素2 mg/kg给药治疗并设置对照组，记录小鼠关节评分；二次免疫70 d后摘眼球取血处死小鼠，ELISA检测小鼠血浆中IL-17、IL-6、TGF-β水平，HE染色法观察小鼠关节病理变化，流式细胞术检测小鼠脾脏细胞中Th17和Treg比例。结果 雷公藤红素组小鼠关节评分显著低于模型组（P<0.01）；HE染色结果显示，雷公藤红素有效改善关节滑膜炎性浸润情况（P<0.05）；ELISA检测小鼠血浆结果显示雷公藤红素使Th17相关细胞因子IL-6水平显著降低（P<0.01)；流式检测结果显示，雷公藤红素可显著降低小鼠脾脏中Th17细胞亚群比例（P<0.01）并增加Treg细胞亚群比例（P<0.05）。结论 雷公藤红素可以有效缓解CIA小鼠关节炎症症状，抑制关节滑膜炎性细胞浸润、血管翳生成和软骨破坏。雷公藤红素治疗CIA小鼠炎症反应可能是通过减少CIA小鼠体内IL-6等促炎症因子的分泌并伴随着抑炎因子参与的免疫平衡调节过程而实现。Abstract: OBJECTIVE To study the anti-inflammatory effect of tripterine on bovine type Ⅱ collagen-induced arthritis in mice and its mechanism of action on T cells. METHODS DBA/1J mice were used to induce CIA model induced by bovine type Ⅱ collagen. After the model was established， tripterine of 2 mg/kg was given to the mice and the joint score was recorded. The mice were sacrificed after 70 d of secondary immunization. The levels of IL-17， IL-6 and TGF-β in the plasma of mice were detected by ELISA. Pathological changes of the joints were observed by HE staining. Flow cytometry was used to detect the ratio of Th17 to Treg in mouse spleen cells. RESULTS The joint scores of tripterine in mice were significantly lower than that in model group (P<0.05). HE staining showed that tripterine improved the synovitis infiltration (P<0.05)； ELISA showed that tripterine significantly decreased the levels of Th17-associated cytokine IL-6 (P<0.01). Flow cytometry showed that tripterine increased the proportion of Treg cells (P<0.05) while significantly decreased the proportion of Th17 cells in the spleen of mice (P<0.01). CONCLUSION Tripthenin can effectively alleviate the symptoms of joint inflammation in CIA mice， inhibit synovitis inflammatory cell infiltration， angiogenesis and cartilage destruction. Tripterine treatment of CIA mice inflammation may be through the reduction of IL-6 secretion in CIA mice with anti-inflammatory cytokines involved in the immune balance regulation process.
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Key words:
- tripterine /
- rheumatoid arthritis /
- Th17 /
- IL-17 /
- IL-6 /
- Treg /
- TGF-β
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