儿童病毒性肺炎风寒郁肺证代谢组学研究

任靖; 汪受传; 张秋月; 翟文生; 赖子娟; 单进军

儿童病毒性肺炎风寒郁肺证代谢组学研究

任靖^1,2,
汪受传^1,2,,
张秋月³,
翟文生³,
赖子娟⁴,
单进军^1,2,

1.
江苏省儿童呼吸疾病中医药重点实验室，南京中医药大学中医儿科学研究所，江苏南京 210023
2.
南京中医药大学医学代谢组学中心，江苏南京 210023
3.
河南中医药大学第一附属医院，河南郑州 450008
4.
美国加州大学戴维斯分校基因组中心，NIH西海岸代谢组学研究中心，美国戴维斯 95616

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出版历程
- 收稿日期: 2016-11-15
- 修回日期: 2017-01-04
- 刊出日期: 2017-03-10

A Metabolomics Study in Pediatric Viral Pneumonia with Syndrome of Wind-Cold

1.
Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing, 210023, China
3.
The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, 450008, China
4.
Genome Center of UC Davis, NIH West Coast Metabolomics Center, Davis, 95616, USA

摘要

摘要: 目的分析病毒性肺炎风寒郁肺证患儿的血清代谢指纹图谱，探讨中医证候的组学代谢物研究方法。方法 30例风寒郁肺证患儿、30例健康儿童血清进行气相色谱-质谱检测，通过在线分析平台XCMS Online预处理、NIST数据库和已有标准品进行验证、MetaboAnalyst确定差异性代谢物及代谢通路。结果有机酸、氨基酸、脂类等差异性代谢物及代谢通路的确定，能够良好区分风寒郁肺证与健康儿童组。与健康儿童相比，风寒郁肺证患儿血清中丙氨酸、天冬氨酸、谷氨酸、甘氨酸、丝氨酸、苏氨酸、牛磺酸、赖氨酸等多种有机酸、氨基酸上调；氨基丁酸、乙醇酸、甘油、1-棕榈酸单甘油酯、胆固醇、葡萄糖等下调，主要涉及丙氨酸、甘氨酸、牛磺酸代谢、氨酰tRNA合成和赖氨酸降解等5条代谢通路异常。结论病毒性肺炎风寒郁肺证存在差异代谢物及代谢通路，这可能与病毒感染导致的机体的多个能量代谢通路相关；代谢组学可为类似中医证候客观化研究提供借鉴。
- 代谢组学 /
- 儿童病毒性肺炎 /
- 风寒郁肺证 /
- 气相色谱-质谱联用
Abstract: OBJECTIVE Discussion on the application of GC-MS to study the metabolic fingerprinting of serum in pneumonia group with syndrome of wind-cold in order to provide data support for the quantitative study of syndromes in TCM. METHODS In the study of patients with wind-cold(WC) in 30 cases，the normal healthy (NH)in 30 cases，Serum samples were collected and serum metabolites profiles were analyzed by GC-MS coupled with a couple of statistical analyses. RESULTS Clear separations between WC and NH existed. Differential metabolites and several metabolic pathways related organic acids，amino acids，fat were identified. Compared with NH group， the levels of organic acids，amino acids，fat were upregulated，such as L-Alanine， L-Aspartic acid， L-Glutamic acid， Glycine， Serine， L-Threonine， Taurine， L-Lysine，and D-2-Aminobutyric acid， Glycolic acid， Glycerol， 1-Monopalmitin， cholesterol， d-Glucose were down in serum with WC group.And， five abnormal metabolic pathways， such as Alanine metabolism，Glycine metabolism， Taurine metabolism，aa-tRNA biosynthesis and Lysine degradation were identified. CONCLUSION This study detected abnormal metabolites and pathways in WC，which may be due to multiple energy metabolism pathways-virus infected;taking advantage of metablomic techniques，we may establish Characterized Spectrum Database in pediatric viral pneumonia and syndrome differentiation pattern standardization of TCM so as to provide the useful methods in study of syndrome standardization.
- Metabolomics /
- viral pneumonia in children /
- syndrome of wind-cold /
- GC-MS

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