骨质疏松症患者破骨细胞相关细胞因子与中医证型的关系
Relationship Between Osteoclast-related Cytokines in Patients with Osteoporosis and TCM Syndromes
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摘要: 目的 探讨骨质疏松症患者破骨细胞相关细胞因子巨噬细胞集落刺激因子(M-CSF)、核因子κB受体活化因子(RANKL)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1α(IL-1α)的表达与中医证型的关联性。方法 将骨质疏松症患者按照中医证型进行辨证分类,随机收集肾阳虚组30例、脾胃虚弱组30例、肝肾阴虚组30例、气滞血瘀组30例,共120例。清晨空腹抽取血液样本,采用酶联免疫法检测M-CSF、RANKL、TNF-α、IL-1α水平。结果 在M-CSF和RANKL的蛋白水平表达上,肾阳虚组患者明显低于其余3组,P<0.01,4组患者在TNF-α和IL-1α蛋白表达水平无统计学差异。结论 可考虑将M-CSF、RANKL的蛋白表达水平作为区别骨质疏松症肾阳虚证与其它3组证型的鉴别方法之一。骨质疏松症在中医辨证分型上具有可被量化的客观依据。Abstract: OBJECTIVE To explore the correlation between the expression of osteoclast-related cytokines like MCSF, RANKL, TNFα IL1αin patients with osteoporosis and TCM syndromes. METHODS 120 cases with osteoporosis were divided into the kidney yang deficiency group(30 cases), deficiency of spleen and stomach group(30 cases), liver-kidney yin deficiency group(30 cases) and qi stagnation and blood stasis group(30 cases) based on syndrome differentiation. Fasting blood samples early in morning were collected and the expression of four items including MCSF, RANKL, TNF and IL1αwere tested by applying euzymelinked immunosorbent assay method. RESULTS Kidney yang deficiency group experienced evident lower expression of MCSF and RANKL than the other three groups, with significantly statistical differences, while no differences were noticed in the expression of TNFαand IL1αamong the four groups. CONCLUSION Detection of MCSF and RANKL expression can be considered as an effective way to distinguish the kidney yang deficiency syndrome from the other three types. Hence, we believe that there exists quantified objective evidence for osteoporosis syndrome differentiation in TCM.
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Key words:
- osteoporosis /
- osteoclast cell /
- TCM syndrome
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[1] COOPER C.Epidemiology and public health impact of osteoporosis[J]. Baillieres Clin Rheumatol, 1993, 7(3): 459-472. [2] DELAET CE, POLS HA. Fractures in the elderly: epidemiology and demography [J]. Best Pract Res Clin Endocrinol Metab, 2000, 14(2): 171-179. [3] 中国老年学学会骨质疏松委员会骨质疏松诊断标准学科组.中国人原发性骨质疏松症诊断标准[J].浙江中西医结合杂志,2007, 17(4):220, 227. [4] Research Group for Diagnostic Criteria of Osteoporosis, Osteoporosis Committee of China Gerontological Society. Diagnostic criteria for Chinese primary osteoporosis[J]. Zhejiang J Integr Tradit Chin West Med, 2007, 17(4): 220, 227. [5] 中药新药临床研究指导原则[M].北京:中国医药科技出版社,2002:385-388. [6] Guiding Principle of Clinical Research on New Drugs of Traditional Chinese Medicine[M]. Beijing: China medical science and technology press, 2002: 385-388. [7] TEITELBAUM SL. Bone resorption by osteoclasts[J]. Science, 2000,289: 1504-1508. [8] BOYLE WJ, SIMONET WS, LACEY DL. Osteoclast differentiation and activation[J]. Nature, 2003, 423(3): 337-342. [9] 董伟,于静,戚孟春,等.M-CSF、RANKL浓度及M-CSF预诱导对破骨细胞生成影响的研究[J].生物医学工程学杂志,2010,27(6):1336-1340. [10] DONG W, YU J, QI MC, et al. Influence of MCSF, RANKL content and MCSF pre-inducing on osteoclastogenesis[J]. J Bio Med Eng, 2010, 27(6): 1336-1340. [11] KANEMATSU M, SATO T, Takai H, et al.Prostaglandin E2 induces expression of receptor activator of nuclear factor-kappa B ligand/osteoprotegrin ligand on pre-B cells: implications for accelerated osteoclastogenesis in estrogen deficiency[J]. J Bone Miner Res, 2000, 15: 1321-1329. [12] 娄志杰,韩向莉,孙勤,等.骨质疏松症中医证型实质的研究思路[J].时珍国医国药,2007, 18(12):3093-3094. [13] LOU ZJ, HAN XL, SUN Q, et al. Research route of TCM syndrome of osteoporosis[J]. Lishizhen Med Mater Med Res, 2007, 18 (12): 3093-3094. [14] 黄宏兴,柴生颋,黄红,等.骨质疏松症中医证型的聚类分析[J].广州中医药大学学报,2007, 24(3):180-183, 187. [15] HUANG HX, CHAI ST, HUANG H, et al. Cluster analysis of TCM syndrome of osteoporosis[J]. J Guangzhou Univ Tradit Chin Med, 2007, 24 (3): 180-183, 187. [16] 张亚军,毕力夫,王琦.绝经后骨质疏松症体质因素的病例对照研究[J].中国骨质疏松杂志,2009, 15(4):296-298. [17] ZHANG YJ, BI LF, WANG Q. Constitution factors for postmenopausal osteoporosis: A case control study[J]. Chin J Osteoporosis, 2009, 15(4): 296-298.
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