艾灸调控IL-17对实验性RA大鼠血清RANKL和TGF-β含量的影响

高梓珊; 甘君学; 郭江燕; 傅淑平; 甘可

艾灸调控IL-17对实验性RA大鼠血清RANKL和TGF-β含量的影响

1.
南京中医药大学第二临床医学院，江苏南京 210023
2.
南京中医药大学第一临床医学院，江苏南京 210023

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出版历程
- 刊出日期: 2016-11-10

Adjustment of Moxibustion on Serum Level of IL-17， RANKL and TGF-β in RA Rats

1.
The Second Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, 210023, China

摘要

摘要: 目的探索艾灸治疗类风湿性关节炎（RA）抗炎的效应及预防骨破坏的IL-17信号转导机制。方法雄性SD大鼠66只，随机分为空白组、模型组、空白艾灸组、艾灸组、IL-17过表达模型艾灸组、IL-17沉默模型艾灸组、IL-17过表达模型组、IL-17沉默模型组，前6组每组各9只，后2组每组各6只。大鼠右后足足垫部皮内注入弗氏完全佐剂0.15mL制作RA模型。造模成功后第2天进行慢病毒注射建立IL-17基因过表达、IL-17基因沉默模型。RA造模后第7天开始麦粒灸大鼠肾俞、足三里2穴各5壮，每日1次，治疗18d。观测计算大鼠足跖肿胀度、热痛域；采用ELISA法检测大鼠血清IL-17、RANKL、TGF-β含量。结果造模后大鼠右后足容积显著高于空白组(P<0.01)，热痛域值显著低于空白组（P<0.01）；艾灸后大鼠右后足容积显著低于模型组(P<0.01)，热痛域显著高于模型组（P<0.01）。模型组大鼠血清IL-17、RANKL显著高于空白组，血清TGF-β含量显著低于空白组(P<0.01)；艾灸后艾灸组大鼠血清IL-17、RANKL显著低于模型组，血清TGF-β含量显著高于模型组（P<0.01）。IL-17过表达模型艾灸组大鼠RANKL含量显著高于艾灸组（P<0.01）；IL-17过表达模型艾灸组大鼠TGF-β含量显著低于艾灸组(P<0.01)。IL-17沉默艾灸组大鼠RANKL含量显著低于艾灸组和空白组(P<0.01)。结论艾灸可能通过调控IL-17，从而调节RA大鼠血清骨破坏因子RANKL以及骨保护因子TGF-β含量，起到抑制RA滑膜炎症后期骨破坏、保护骨组织的作用。
- 艾灸 /
- RA /
- IL-17 /
- TGF-β /
- RANKL /
- 骨破坏
Abstract: OBJECTIVE To explore the IL-17 signal transduction mechanism of anti-inflammatory effect of moxibustion on RA rats. METHODS 66 SD rats were randomly assigned to control， model， control+moxa， moxa， IL-17 overexpression+moxa， IL-17 knockdown+moxa， IL-17 overexpression and IL-17 knockdown groups. Complete freund's adjuvant were injected into the right paw of SD rats to establish RA model， and siRNAs were injected for IL-17 overexpression/knockdown models. After 7d， moxibustion treatments were performed for 18d. RESULTS The swelling degree of RA rats and the serum level of IL-17 and RANKL were significantly higher than control group(P<0.01)， and the PWTL of RA rats，serum level of TGF-β were significantly lower than control group. After 18d of moxibustion treatment， the swelling degree of RA rats and the serum level of IL-17，RANKL were significantly lower than control group(P<0.01)，and the PWTL of RA rats and serum level of TGF-β were significantly higher than control group. In addition， the serum level of RANKL in IL-17 knockdown+moxa rats were significantly higher than moxa group(P<0.01); the serum level of RANKL in IL-17 knockdown+moxa rats were significantly lower than moxa group and control group（P<0.01）. CONCLUSION Moxibustion may adjust the serum level of RANKL and TGF-β by supressed the serum level of IL-17 therefore relieving RA in RA rats.
- moxibustion /
- RA /
- IL-17 /
- TGF-β /
- RANKL /
- bone destruction