5-氟尿嘧啶对正常小鼠脾虚证候的影响研究

郭锦瑞; 葛飞; 康安; 王小龙

5-氟尿嘧啶对正常小鼠脾虚证候的影响研究

郭锦瑞¹,
葛飞^2,3,
康安¹,
王小龙^1,

1.
南京中医药大学药学院，江苏南京 210023
2.
南京中医药大学第一临床医学院，江苏南京 210023
3.
海安县中医院，江苏海安 226600

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出版历程
- 收稿日期: 2013-09-30
- 修回日期: 2013-12-29
- 刊出日期: 2014-03-10

Effects of 5-Fu on Mice with Spleen Deficiency Syndrome

1.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
The First Medical College, Nanjing University of Chinese Medicine, Nanjing, 210023, China
3.
Hai'an Hospital of Traditional Chinese Medicine, Hai'an, 226600, China

摘要

摘要: 目的研究化疗药物5-Fu对小鼠脾虚证候及肠内容物中短链脂肪酸含量的影响。方法正常小鼠腹腔注射5-Fu后统计小鼠摄食量、体质量、腹泻等数据，观察其脾虚证候表现，同时通过做HE染色，肠黏膜β-D-半乳糖苷酶活性等评价其胃肠道毒性，并检测肠内容物中短链脂肪酸的含量，探讨5-Fu对肠道菌群的影响。结果与正常组相比5-Fu引起小鼠摄食减少、体质量下降、腹泻指数增加，病理切片结果表明小肠绒毛变短，病变程度增加，血浆中TNF-α和IL-6含量增加，肠黏膜β-D-半乳糖苷酶活性降低，肠内容物中乙酸、丁酸、戊酸浓度显著降低。结论 5-Fu可致小鼠脾虚证候，对肠黏膜及肠道菌群损伤明显，提示在应用化疗药物5-Fu时，可联合应用健脾中药，减低其胃肠道毒副作用。
- 5-Fu /
- 脾虚证候 /
- 短链脂肪酸
Abstract: OBJECTIVE To study the effects of 5-Fu on mice with spleen deficiency syndrome and the content of short chain fatty acid in intestine. METHODS After the injection of 5-Fu in abdomen， data such as food intake， body weight changes， diarrhea and other information were collected to observe symptoms due to spleen deficiency. Endo-intestinal toxicity of 5-Fu were evaluated by HE staining and detecting beta-D-galactose glucoside enzyme activity in intestinal mucosa at the same time. The content of short chain fatty acids in intestinal contents were also determined to investigate the influences of 5-Fu on the intestinal flora. RESULTS Compared with control group， the spleen deficiency syndrome may induced by 5-Fu， the mice appeared the symptoms， such as poor appetite， weight loss and diarrhea， and it was shown in the physiological section that the intestine villis significantly shortened， the pathological changes further developed， the contents of TNF-α and IL-6 in plasma increased and the beta-D-galactose glucoside enzyme activity of intestinal mucosa decreased. Contents of acetic acid， butyric acid and pentanoic acid in intestine significantly reduced at the same time. CONCLUSION Fu may induce spleen deficiency syndrome in mice and significantly damage the intestinal mucosa and intestinal flora， which indicate that when 5-Fu is applied， some spleen-strengthening medicine may be used to reduce induced intestinal side effects.
- 5-Fu /
- spleen deficiency syndrome /
- short-chain fatty acids

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参考文献(13)

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