当归补血汤及拆方对大鼠免疫性肝损伤的治疗作用

孙丽霞; 方南元; 周玲玲; 柳璋璞

当归补血汤及拆方对大鼠免疫性肝损伤的治疗作用

1.
南京中医药大学附属医院，江苏南京 210029
2.
南京中医药大学药学院，江苏南京 210023

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出版历程
- 收稿日期: 2013-11-08
- 修回日期: 2013-12-19
- 刊出日期: 2014-03-10

Therapeutic Effects of Danggui Buxue Decoction and its Decomposition on Rats with Immunological Liver Injury

1.
The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China
2.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China

摘要

摘要: 目的观察当归补血汤及拆方对免疫性肝损伤大鼠的肝功能、肝纤维化的影响。方法采用猪血清腹腔给药方法，造成免疫性肝损伤模型，检测大鼠血清中丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、超氧化物歧化酶(SOD)、脂质过氧化物丙二醛(MDA)、透明质酸（HA）、IV型胶原、III型前胶原。结果黄芪/当归5∶1组可明显降低猪血清所致免疫性肝损伤大鼠的血清AST、ALT含量；黄芪组、当归组及黄芪当归不同配比组均能升高SOD水平，降低MDA的含量；黄芪组能明显降低HA、IV型胶原；当归补血汤5∶1组能明显降低III型前胶原水平，且优于10∶1组。结论当归补血汤对免疫性大鼠肝损伤模型具有一定保护作用，其作用机制可能与降低、抑制脂质过氧化反应以及抗自由基损伤有关。
- 当归补血汤 /
- 大鼠 /
- 免疫性肝损伤 /
- 药效学 /
- 模型
Abstract: OBJECTIVE To observe effects of Danggui Buxue Decoction and the composition on liver function and liver fibrosis in rats with immunological liver injury. METHODS The immunological liver injury models were established with intraperitoneal administration of pig serum， whose alanine aminotransferase (ALT)， aspartate aminotransferase(AST)， superoxide dismutase(SOD)， lipid peroxide malondialdehyde (MDA)， Hyaluronic acid (HA)， IV collagen and III procollagen were detected. RESULTS The group with ratio of Astragalus root to Chinese angelica 5:1 significantly decreased AST and ALT levels; Astragalus root group， Chinese angelica group and groups with different ratios of Astragalus root to Chinese angelica increased SOD levels and decreased MDA content; Astragalus root group obviously reduced HA and type-IV collagen; Danggui Buxue Decoction group with the ratio 5:1 significantly reduced type-III procollagen levels， superior to that in group with ratio10:1. CONCLUSION Danggui Buxue Decoction makes a certain effect on rats with immunological liver injury， whose mechanism may be related to reduction and inhibition of lipid peroxidation and anti-free radical damage.
- Danggui Buxue Decoction /
- rat /
- immunological liver injury /
- pharmacodynamics /
- model

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参考文献(19)

[1]	陶艳艳，陈园，陈高峰，等．当归补血汤不同配比组方的抗肝纤维化作用[J]．上海中医药大学学报，2008， 22（1）：40-44．
[2]	Tao YY， Chen Y， Chen GF， et al. Anti-hepatic Fibrosis Effect of "Danggui Buxue Decoction" with Different Proportions of Radix Astraga and Angelica Sinensis in Rats[J]. Acta Univ Tradit Med Sin Pharmacol Shanghai， 2008， 22(1): 40-44.
[3]	袁国红，顾立刚．当归补血汤对血细胞及相关免疫因子的作用[J]．中国组织工程研究与临床康复，2007， 11（28）：5606-5609．
[4]	Yuan GG， Gu LG. Effect of danggui buxue tang on blood cells and some relative active factors[J].Chin J Tis Engineer Res， 2007， 11(28): 5606-5609.
[5]	Islam MN， Choi RJ， Jin SE， et al. Mechanism of anti-inflammatory activity of umbelliferone 6-carboxylic acid isolated from Angelica decursiva[J]. J Ethnopharmacol， 2012， 144(1): 175-181.
[6]	Han C GJ， pairs activity of traditional Chinese herb anti-inflammatory. Angelica sinensis and Sophora flavescens[J]. Inflammation， 2012， 35(3): 913-919.
[7]	Lee HJ， Choi TW， Kim HJ， et al. Anti-Inflammatory activity of angelica keiskei through suppression of mitogen-activated protein kinases and nuclear factor-kappaB activation pathways[J]. J Med Food， 2010， 13(3): 691-699.
[8]	刘雅，张海港，张翼冠，等．当归补血汤对气虚血瘀大鼠免疫功能及相关基因的调控[J]．中药药理与临床，2009， 25（5）：10-13．
[9]	Liu Y， Zhang HG， Zhang YG， et al.The regulation effects of Danggui Buxue Decoction on immune function of rats with qi deficiency and blood stasis and related genes[J]. Pharmocol Clin Chin Mater Med， 2009， 25(5): 10-13.
[10]	苗明三，方晓艳．当归补血汤粗多糖对免疫抑制小鼠免疫功能的影响[J]．中药药理与临床，2003， 19（6）：8-9．
[11]	Miao MS， Fang XY. Effects of Dangguibuxuetang polysaccharide on immunological functions of immunosuppressional model mice[J]. Pharmocol Clin Chin Mater Med， 2003， 19(6): 8-9.
[12]	Chang MS， Kim do R， Ko EB， et al. Treatment with Astragali radix and Angelicae radix enhances erythropoietin gene expression in the cyclophosphamide-induced anemic rat[J]. J Med Food， 2009， 12(3): 637-642.
[13]	Hui MK， Wu WK， Shin VY， et al. Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice[J]. Int J Med Sci， 2006， 3(1): 1-6.
[14]	陈奇．中药药理研究方法学[M]．北京：人民卫生出版社，1993：851-853．
[15]	Chen Q．The research on traditional Chinese medicine pharmacology's methodology[M]. Beijing: people's medical publishing house， 1993:851-853.
[16]	Gossard AA， Lindor KD. Autoimmune hepatitis: a review[J]. J Gastroenterol， 2012， 47(5): 498-503.
[17]	Achenza MI， Meda F， Brunetta E， et al. Serum autoantibodies for the diagnosis and management of autoimmune liver diseases[J]. Expert Rev Gastroenterol Hepatol， 2012， 6(6): 717-729.
[18]	肖柳英，潘竞锵，饶卫农，等．补阳还五汤对小鼠免疫性肝炎及急性肝损伤的保护作用研究[J]．中医研究，2005， 18（6）：13-14．
[19]	Xiao LY， Pan JQ， Rao WN， et al. Protection of the wuyang huanwu decoction from immune hepatitis and acute liver trauma in mice[J]. Tradit Chin Med Res， 2005， 18(6): 13-14.