通塞脉制剂对过氧化氢致PC12细胞损伤的保护作用

李锦吉; 狄留庆; 李俊松; 毕肖琳

通塞脉制剂对过氧化氢致PC12细胞损伤的保护作用

李锦吉^1,2,
狄留庆^1,2,,
李俊松^1,2,
毕肖琳^1,2

1.
南京中医药大学药学院，江苏南京，210023
2.
江苏省中药高效给药系统工程技术研究中心，江苏南京 210023

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出版历程
- 收稿日期: 2013-11-19
- 修回日期: 2014-02-10
- 刊出日期: 2014-03-10

The Protective Effect of Tongsaimai Decoction on PC 12 Cells Induced by H₂O₂

1.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
Jiangsu Province Engineering Research Center for Chinese Medicine High Efficiency Drug Delivery System, Nanjing, 210023, China

摘要

摘要: 目的观察通塞脉制剂对过氧化氢（H₂O₂）作用下PC12细胞的保护作用。方法体外培养PC12细胞，将细胞分为正常对照组，过氧化氢组，通塞脉高剂量组(5 mg/mL)，通塞脉中剂量组(0.5 mg/mL)，通塞脉低剂量组(0.05 mg/mL)。200 μmol/L过氧化氢被加入作为刺激物孵化4 h后，再加入终浓度5、0.5、0.05 mg/mL的药物，继续培养24 h，用MTT法测定并计算药物对H₂O₂致PC12细胞损伤的保护率；取各组细胞上清液，按照试剂盒的操作方式，测定超氧化物歧化酶(SOD)，丙二醛(MDA)，一氧化氮(NO)，乳酸脱氢酶(LDH)及钙离子含量(Ca²⁺)。结果通塞脉制剂(5、0.5、0.05 mg/mL)对过氧化氢致PC12细胞损伤均有保护作用。过氧化氢组细胞上清液中SOD活性，SOD抑制率降低(P<0.05~0.01)，MDA，LDH，NO含量与Ca²⁺含量增加(P<0.05~0.01)。而各给药组能提高SOD活性及抑制率，降低LDH，MDA，NO以及Ca²⁺含量，与过氧化氢组比较有显著性差异(P<0.05~0.01)。结论通塞脉制剂对PC12细胞损伤有显著性保护作用，其机制与钙超载，氧化应激等因素有关。
- 通塞脉 /
- 过氧化氢 /
- PC12细胞 /
- 氧化应激
Abstract: OBJECTIVE To observe the protective effects of Tongsaimai decoction preparation on the PC12 cells induced by H₂O₂. METHODS The in-vitro cultured PC12 cells were randomly divided into eight groups such as normal group， hydrogen peroxide group， Tongsaimai decoction with high dose group(5 mg/mL)， Tongsaimai decoction with middle dose group(0.5 mg/mL)，Tongsaimai decoction with low dose group(0.05 mg/mL). After the 200 μmol/L H₂O₂was added to stimulate the PC12 cells and incubated for 4 h， then the final concentration of drugs (5 mg/mL， 0.5 mg/mL， 0.05 mg/mL) were added and continuously incubated and cultured for 24 h. The MTT assay was facilitated to measure and calculate the cell viability the protection rate of the drugs on PC 12 cells induced by hydrogen peroxide. Finally， the corresponding biochemical indicators like SOD， MDA，NO， LDH and Ca²⁺ were measured. RESULTS The Tongsaimai decoction(5 mg/mL， 0.5 mg/mL， 0.05 mg/mL) possess protective effects on H₂O₂-cellular injury model group induced to PC12 cells injury. The hydrogen peroxide model group revealed that could reduce the SOD activity and SOD inhibition rate(P<0.05~0.01)， conversely could increase the MDA， NO content， LDH activity and Ca²⁺ levels(P<0.05~0.01).Each dose of drugs groups could increase the SOD activity and SOD inhibition rate， conversely could reduce the LDH activity， NO content， MDA and Ca²⁺ levels when compared with the hydrogen peroxide model group showing the significant differences(P<0.05~0.01). CONCLUSION Tongsaimai decoction has revealed the significant protective effects towards the PC12 cells injury， its mechanisms are closely-related to the calcium overload， the oxidative stress and others.
- Tongsaimai /
- H₂O₂ /
- PC12 /
- oxidative stress

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