川芎水提部位化学成分的UPLC-ESI-Q-TOF-MS分析
Analysis on Chemical Components from Water Extract of Ligusticum Chuanxiong by UPLC-ESI-Q-TOF-MS
-
摘要: 目的 采用UPLC-ESI-Q-TOF-MS技术对川芎水提取部分中的化学成分进行定性分析。方法 采用LC-MS/MS,C18色谱柱(4.6 mm×250 mm,5 μm)以甲醇-0.1%甲酸溶液为流动相梯度洗脱;质谱使用ESI离子源,正离子与负离子模式下采集数据。结果 通过正、负离子质谱信息及元素组成,分析与相关文献数据对照,共鉴定出13个化合物。结论 通过超高效液相色谱分离,质谱测定相对分子质量及相关文献数据信息检索确定川芎水提取部分中的化学成分,为阐明川芎水提部分的药效物质基础提供有力的证据。
-
关键词:
- 川芎 /
- 化学成分 /
- UPLC-ESI-Q-TOF-MS
Abstract: OBJECTIVE To make a qualitative analysis on chemical components in water extract of Ligusticum chuanxiong by UPLC-ESI-Q-TOF-MS.METHODS LC-MS/MS, C18(4.6 mm×250 mm,5 μm) column was adopted,with methanol -0.1% formic acid as the mobile phase for gradient elution; ESI ion source was used for mass spectra,and data were collected in positive and negative mode.RESULTS By analyzing positive and negative ion mass spectra information, element composition as well as comparing controls with data from relevant literature, thirteen compounds from water extract of Ligusticum chuanxiong Hort were identified. CONCLUSION After the separation by ultra high performance liquid chromatography, relative molecular mass was determined by mass spectra, and chemical compounds in water extract of Ligusticum chuanxiong Hort were determined by information search through relevant literature data, which served as a powerful proof for pharmacodynamic material basis of water extract from Ligusticum chuanxiong. -
[1] 王英婷,韩勇.川芎药材HPLC法指纹图谱的研究[J].中国药事, 2011,25(10):1017-1021. [2] Wang YT, Han Y. Study on Fingerprint of ligusticun chuanxiong Hort by HPLC[J]. Chin Pharm Aff, 2011,25(10): 1017-1021. [3] 左爱华,王 莉,肖红斌.洋川芎内酯 A 和洋川芎内酯Ⅰ的降解产物研究[J].中草药,2012, 43(11):2127-2131. [4] Zuo AH, Wang L, Xiao HB. Study of degradation products of rhizoma ligustici wallichii lactone A and rhizoma ligustici wallichii lactone Ⅰ[J]. Chin Herb Med, 2012,43(11):2127-2131. [5] 杨丽红,谢秀琼,万丽,等.川芎化学成分研究[J].时珍国医国药,2007, 18(7):1576-1577. [6] Yang LH, Xie XQ, Wan L, et al. Chemical constituents from Ligusticum chuanxiong Hort[J]. Lishizhen Med Mater Med Res, 2007, 18(7): 1576-1577. [7] 石世学,潘勤,元英群,等.GC-MS法建立都江堰产川芎挥发油的指纹图谱[J].中草药,2007, 38(8):1177-1180. [8] Shi SX, Pan Q, Yuan YQ, et al. Fingerprint of essential oil in Rhizoma Chuanxiong from Dujiangyan by GC-MS[J]. Chin Herb Med, 2007, 38(8): 1177-1180. [9] 李艳辉,易进海,官艳丽,等.超临界萃取川芎精油的GC-MS分析[J].分析试验室,2007, 26(5):106-108. [10] Li YH, Yi JH, Guan YL, et al. GC-MS analysis of essential oil from the roots of ligusticum chuanxiong with supercritical fluid extraction[J]. Chin J Anal Lab, 2007, 26(5): 106-108. [11] 任东春,杨念云,钱士辉,等.川芎地上部分化学成分研究[J].中国中药杂志,2007, 32(14):1418-1420. [12] Ren DC, Yang NY, Qian SH, et al. Chemical study on aerial parts of Ligusticum chuanxiong[J]. Chin J Chin Mater Med, 2007, 32(14): 1418-1420. [13] 谢静,张浩,易涛,等.高效液相和气质联用色谱法检测不同方法提取川芎挥发油的化学成分[J].中国医院药学杂志,2008,28(6):418-421. [14] Xie J, Zhang H, Yi T, et al. HPLC and GC-MS detection of essential oil of Rhizoma Chuanxiong extracted using different methods[J]. Chin J Hosp Pharm, 2008, 28(6):418-421. [15] 夏宇,徐如彦,张峻松.川芎油挥发性成分分析及在卷烟中的应用[J].农产品加工:学刊,2011(12):73-75,78. [16] Xia Y, Xu RY, Zhang JS. Analysis of volatile constituents in ligusticum chuanxiong Hort oil and its application in cigarettes flavoring[J]. Acad Periodical Farm Prod Process, 2011(12): 73-75,78. [17] 郝淑娟,张振学,田洋,等.川芎化学成分研究[J].中国现代中药,2010, 12(3):22-25,38. [18] Hao SJ, Zhang ZX, Tian Y, et al. Study on chemical constituents of ligusticum chuanxiong[J]. Mod Chin Med, 2010, 12(3): 22-25,38.
点击查看大图
计量
- 文章访问数: 710
- HTML全文浏览量: 10
- PDF下载量: 943
- 被引次数: 0