灵巴菌质碱性醇提物体外抑制A549细胞增殖的作用及机制
Proliferative inhibition effects of Lingba Junzhi alkaline constituents on human non-small cell lung cancer A549 cells and its mechanism
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摘要: 目的 通过体外细胞培养探讨灵巴菌质的碱性醇提物对人肺癌细胞A549增殖的抑制作用及相关机制。方法 对灵巴菌质的甲醇提取部位采用酸溶碱沉-溶剂萃取等方法处理,得到其碱性醇提物,HPLC法定性鉴定巴豆苷的存在。进而通过MTT比色法、软琼脂克隆形成法、PI单染法和免疫荧光染色法观察其对A549细胞的增殖抑制、成瘤能力、生长周期分布及细胞骨架损伤程度的影响。结果 灵巴菌质的碱性醇提物含有抗癌活性成分巴豆苷,它能够显著抑制A549细胞的增殖,降低细胞克隆形成的能力,能将这种细胞的生长阻滞在G2期,并对其细胞骨架造成一定的损伤。结论 灵巴菌质的碱性醇提物具有体外抑制A549细胞增殖的效应,其作用机制可能与降低细胞克隆形成能力及损伤细胞骨架有关。Abstract: OBJECTIVE To explore the proliferative inhibition effects of Lingba Junzhi alkaline constituents on human non-small cell lung cancer A549 cells and its mechanism via in vitro cell culture.METHODS The alkaline constituents were obtained by alkali-solution and acid-isolation extraction, and solvent extraction of Lingba Junzhi methyl alcohol extract parts; HPLC was applied to verify the existence of Crontonside. MTT assay, soft agaragar assay, PT single-stain and immunofluorescent staining were later used to observe its effects on inhibiting A549 cell proliferation, tumor formation, growth cycle distribution and cytoskeleton destruction.RESULTS Crontonside did exist in Lingba Junzhi alkaline constituents, which significantly inhibited A549 proliferation and reduced cell clone formation, delayed the cell circle in G2 period and caused certain damage to the cytoskeleton.CONCLUSION Lingba Junzhi alkaline constituents can inhibit in vitro A549 cell proliferation and its mechanism may be related to its decreasing clone formation ability and damaging cytoskeleton.
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Key words:
- Croton tiglium /
- fermentation /
- alkaline constituents /
- A549 cells /
- anti-tumor /
- mechanism
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