常春藤皂苷元对结肠癌细胞LoVo增殖、粘附、侵袭和迁移能力的影响
The Effect of Hederagenin on the Proliferation， Adhesion， Invasion and Migration of Human Colon Cancer Cells LoVo
-
摘要: 目的 常春藤皂苷元体外对人结肠癌细胞LoVo增殖、粘附、侵袭和迁移能力的影响。方法 采用MTT法、粘附实验、Transwell小室侵袭实验、划痕实验，观察常春藤皂苷元对人结肠癌细胞LoVo增殖、粘附、侵袭和迁移能力的影响。结果 ①常春藤皂苷元有抑制人结肠癌细胞LoVo增殖的作用，与对照组比较，随着药物浓度的增大，其对细胞的抑制率也明显增大(P<0.01)，呈浓度依赖性。②常春藤皂苷元作用人结肠癌细胞LoVo后，细胞的粘附能力明显降低(P<0.01)，随着浓度的增大和时间的推移，呈现抑制率递增的结果。③常春藤皂苷元作用人结肠癌细胞LoVo后，侵袭的细胞数较对照组明显减少(P<0.01)，浓度越高，侵袭的细胞数量越少。④常春藤皂苷元作用人肠癌LoVo细胞后，细胞愈合的程度有明显的分组差别，浓度越高，愈合度越差，表明迁移能力受到很大影响。结论 常春藤皂苷元对人结肠癌细胞LoVo的增殖具有抑制作用；常春藤皂苷元能抑制人结肠癌细胞LoVo的粘附能力、侵袭能力和迁移能力。Abstract: OBJECTIVE To explore the effect of hederagenin on the proliferation， adhesion， invasion and migration of human colon cancer cell line LoVo in vitro.METHODS MTT assay， adhesion experiment， Transwell room invasion test， and erasion trace test were used to observe the effect of hederagenin on the proliferation， adhesion， invasion and migration.RESULTS ①Hederagnin inhibited the proliferation of LoVo cells. Compared with control group， the inhibition ratio had been significantly increasing with the increase of the drug concentration， which showed a concentration dependence (P<0.01). ②Hederagnin remarkably reduced the adhesion capability of LoVo cells (P<0.01)， and along with the increasing drug concentration and time， the inhibition ratio increased. ③Compared with control group， the number of invaded cells was significantly decreased (P<0. 01). The higher the drug concentration was， the less cells were invaded. ④The cell healing degree had an obvious group difference， and the higher the drug concentration was， the worse the healing degree was， which indicated that migration ability was greatly affected.CONCLUSION Hederagenin can remarkably inhibit the proliferation， adhesion， invasion and migration of LoVo cells.
-
Key words:
- hederagenin /
- LoVo /
- proliferation /
- adhesion /
- invasion /
- migration
-
[1] 李佳，刘继永. 中药威灵仙的化学成分及药理作用研究进展[J]．特产研究，2011(1)：69-75． [2] Li J， Liu JY. Research progress On chemical constituents and pharmacological effects of traditional Chinese medicine Clematis Chinensis[J]. Special Wild Economic Animal and Plant Res， 2011(1): 69-75. [3] 赵燕强，杨立新，张宪民，等．威灵仙的成分、药理活性和临床应用的研究进展[J]．中药材，2008， 31（3）：465-470． [4] Zhao YJ， Yang LX， Zhang XM， et al. Research progress On chemical constituents， pharmacological effects and clinical application of Clematis Chinensis[J]. J Chin Med Mater， 2008， 31(3): 465-470. [5] 黄志勤，李洪亮，孙立波，等．齐墩果酸抗大鼠移植性肝癌作用的研究[J]．安徽农业科学，2011， 39（18）：10807-10808，10851． [6] Huang ZQ， Li HL， Sun LB， et al. Study on inhibitive effect of oleanolic acid on transplanted hepatocarcinoma in mice[J]. J Anhui Agricult Sci， 2011， 39(18): 10807-10808，10851. [7] 王晶，张涛，商云飞．齐墩果酸对宫颈癌Hela细胞凋亡的影响[J]．黑龙江医药科学，2011， 34（1）：31-32． [8] Wang J， Zhang T， Shang YF. Effect of oleanolic acid on Hela cells:An experimental study[J]. Heilongjiang Med Pharm， 2011， 34(1): 31-32. [9] 邱光清，张敏，杨燕军．威灵仙总皂甙的抗肿瘤作用[J]．中药材，1999， 22（7）：353． [10] Qiu GQ， Zhang M， Yang YJ. The anti-cancer effect of clematis root total saponins[J]. J Chin Med Mater， 1999， 22(7): 353. [11] 赵英，余春粉．张桂英，等.威灵仙总皂苷抗肿瘤作用及其对癌细胞增殖周期的影响[J]．时珍国医国药，2010， 21（8）：1908-1909． [12] Zhao Y， Yu CF， Zhang GY， et al. The anti-cancer effect of Clematis root total saponins and its influence on proliferation cycle of oncocytes[J]. Lishizhen Med &Mat Med Res， 2010， 21(8): 1908-1909. [13] Wilkes G. Kevan hartshorn colon rectal and analcancers[J]. Semin Oncol Nurs， 2009， 25(1): 32-47. [14] Gassmann P， Enns A， Haier J. Role of tumor cell adhesion and migration in organ-specific metastasis formation[J]. Onkologie， 2004， 27(6): 577-582. -
点击查看大图
计量
- 文章访问数: 646
- HTML全文浏览量: 3
- PDF下载量: 604
- 被引次数: 0
下载:
