柿叶总黄酮对apoE-/-小鼠动脉粥样硬化病变中粘附分子表达的影响
Effect of Total Flavonoids in Persimmon Leaf on the Expression of Arteriosclerosis ApoE-/- mice's Adhesion Molecule
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摘要: 目的 探讨柿叶总黄酮对载脂蛋白E基因敲除小鼠胸腹主动脉粥样硬化（Atherosclerosis， AS）斑块中粘附分子表达的影响。方法 ApoE-/-小鼠随机分为模型组和黄酮组，C57BL/6小鼠作为对照，胸腹主动脉近心端连续冷冻切片，苏木素伊红(HE)染色观察组织形态学变化，血清检测观察血脂变化，免疫组化染色观察粘附分子在病变部位的表达分布。结果 HE染色结果显示，正常对照组血管内膜平滑，无AS病变，模型组大部分斑块面积较大，突入管腔致管腔明显狭窄，黄酮组大部分病变较轻，无明显管腔狭窄;血脂检测表明，模型组和黄酮组之间血脂水平无显著性差异;免疫组化结果显示，正常对照组小鼠主动脉无阳性染色颗粒表达，模型组斑块的肩部、纤维帽部有较多阳性染色颗粒表达，而黄酮组阳性染色颗粒较少，主要表达于血管内膜，且模型组显著低于黄酮组，差异有显著性（P<0.05）。结论 柿叶总黄酮可在一定程度上抑制P-Selectin、ICAM-1、VCAM-1等粘附分子表达，从而减缓AS病变，但并不依赖于其对血脂的降低作用。Abstract: OBJECTIVE To explore the effect of total flavnoids of persimmon leaves on the expression of adhesion molecule in ApoE-/- mice with thoracic and abdominal atherosclerosis.METHODS ApoE-/- mice were divided into model group and total treatment group randomly， putting c57BL/6 mice as control group， making the proximal part of the thoracic and abdominal aorta into continuous frozen section， which would be stained and observed about the tectology changes. The serum changes would also be tested. Immunohistochemistry stain method was used to observe the distribution of the adhesion molecule expression in the certain pathological location. RESULTS HE stained showed that the control group's enangium is smooth without AS changes， and the model group had large area plaques， extending into the lumen and caused stenosis. The treatment group alleviated without obvious lumen stenosis. The serum tests show there was no apparent lipdemia differences between the model group and treatment group. The immunohistochemisty tests show the control group mice aorta had no positive stained granule expression， and the model group had the positive expression in the shoulder and the fiber head of the plaque. The treatment group had a rare positive expression which mainly intra the vessel. The model group were lower than the treatment group apparently (P<0.05).CONCLUSION Total flavnoids of persimmon leaves can suppress the expression of adhesive molecule (P-Selection， ICAM-1， VCAM-1， et al). Furthermore to alleviate the pathogenic changes of AS， but it cannot lower the lipdemia.
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