慢性抑郁症小鼠巨噬细胞相关因子的变化及针刺调节作用
Alternation of Macrophage Cytokines in the Progress of Mice Depression and the Regulation of Acupuncture
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摘要: 目的 探讨巨噬细胞相关细胞因子在小鼠抑郁症发生发展过程中的变化，并研究针刺治疗抑郁症的免疫学机制。方法 采用慢性不可预见性中等强度应激（CUMS）造成小鼠抑郁症模型，以开场试验、糖水偏好试验作为行为学评价指标；同时在造模过程中不同时间点，取小鼠腹腔巨噬细胞，培养并以LPS刺激，用ELISA法测定上清液中TNF-α、IL-1β、IL-6的含量；从造模成功开始对模型小鼠进行针刺治疗，观察治疗不同时间后模型小鼠行为学的改变情况，并测定小鼠腹腔巨噬细胞分泌细胞因子的含量。结果 造模21 d后，小鼠糖水偏好、开场实验水平运动、垂直运动等明显下降，说明抑郁样症状已形成。在模型形成过程中，巨噬细胞分泌IL-1β、IL-6能力在造模的中后期显著提高，TNF-α的能力有提高趋势但未见显著差异。针刺治疗后，在改变行为学指标的同时，对巨噬细胞分泌TNF-α、IL-1β、IL-6有下调作用。结论 小鼠抑郁症形成过程中伴随着巨噬细胞分泌炎性细胞因子的升高，而针刺对此的调节可能是其改善行为学的作用机制之一。Abstract: OBJECTIVE To study the changes of macrophage-related cytokines in the development of depression， and to explore the immunological mechanism of acupuncture on treating depression.METHODS Chronic unpredictable mild stress (CUMS) was employed to induce depression in mice and open-field test and sucrose preference test were operated during the progress of depression. Simultaneously， the peritoneal macrophages were isolated from mice and were cultured under the stimulation with LPS， ELISA method were used to measure the contents of TNF-α， IL-1β and IL-6 in the supernatant of peritoneal macrophages. Depression mice were given treatment of acupuncture， and the effect of behavior change was observed and the content of macrophage cytokine production was measured respectively.RESULTS 21 days after making model， the model of chronic unpredictable mild depression was generally developing， the secretion ability of IL-1β and IL-6 in the middle or late stage increased significantly， while the ability of TNF-α did increase but not that obvious. After treatment of acupuncture， behavior indicator was improved and meanwhile the ability of secreting TNF-α， IL-1β， IL-6 decreased significantly. CONCLUSION The ability of modeling mice secreting inflammatory cytokines by peritoneal macrophages increased in the formation of CUMS depression， which acupuncture can significantly regulate might be one of the mechanisms in improving behavior change.
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Key words:
- depression /
- macrophage /
- interleukins /
- acupuncture
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[1] Smith RS. The macrophage theory of depression[J]. Med Hypotheses， 1991， 35(4): 298-306. [2] 王胜．抑郁症针灸治疗思路探析[J]．中医药学报，2007， 35（5）：44-45． [3] Wang S. Thoughts on depression treatment using acupuncture[J]. Acta Chin Med Pharmacol， 2007， 35(5): 44-45. [4] Willner P， Towell A， Sampson D， et al. Reduction of sucrose preference by chronic unpredictable mild stress， and its restoration by a tricyclic antidepressant[J]. Psychopharmacology (Berl)， 1987， 93(3): 358-364. [5] Kitayama I， Wang P， Yanashita K， et al. Noradrenergic function in depression model rats. In:Nomura J，ed.Neurobiology of depression and related disorders[M].New York:Acad Press，1998:236-247. [6] Owen BM， Eccleston D， Ferrier IN， et al. Raised levels of plasma interleukin-1beta in major and postviral depression[J]. Acta Psychiatr Scand， 2001， 103(3): 226-228. [7] Einvik GVM， Hrubos-Strom H， Randby A， et al. Circulating cytokine concentrations are not associated with major depressive disorder in a community-based cohort[J]. Gen Hosp Psychiatry， 2012， 34(3):262-267. -
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