采用体外肠道菌群实验研究防风对黄芪中毛蕊异黄酮葡萄糖苷代谢的影响

刘玲; 赵晓莉; 狄留庆; 李俊松

采用体外肠道菌群实验研究防风对黄芪中毛蕊异黄酮葡萄糖苷代谢的影响

刘玲¹,
赵晓莉^1,2,,
狄留庆^1,2,
李俊松¹

1.
南京中医药大学药学院，江苏南京 210023
2.
江苏省中药高效给药系统工程技术研究中心，江苏南京 210023

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出版历程
- 收稿日期: 2014-12-24
- 修回日期: 2015-01-13
- 刊出日期: 2015-03-10

Effects of Radix Saposhnikoviae on Metabolism of Calycosin-7-O-β-D-glucoside in Radix Astragali by Intestinal Flora Experiment in vitro

1.
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
Jiangsu Engineering Research Center for Efficient Delivery System of TCM, Nanjing, 210023, China

摘要

摘要: 目的研究防风对黄芪中主要活性成分毛蕊异黄酮葡萄糖苷在大鼠肠道菌群中代谢的影响，从代谢的角度探讨黄芪-防风药对配伍应用的科学内涵。方法通过肠道菌群实验比较不同时间点黄芪组和黄芪-防风组以及毛蕊异黄酮葡萄糖苷单体组和毛蕊异黄酮葡萄糖苷-防风组中毛蕊异黄酮葡萄糖苷、毛蕊异黄酮的浓度。结果与黄芪组相比，不同时间点黄芪-防风组毛蕊异黄酮葡萄糖苷的浓度均增高，毛蕊异黄酮的浓度均降低。与毛蕊异黄酮葡萄糖组相比，不同时间点毛蕊异黄酮葡萄糖苷-防风组毛蕊异黄酮葡萄糖苷的浓度均增高，毛蕊异黄酮的浓度均降低。结论防风能抑制黄芪中毛蕊异黄酮葡萄糖苷转化为毛蕊异黄酮的水解反应，减缓其代谢。
- 防风 /
- 黄芪 /
- 毛蕊异黄酮葡萄糖苷 /
- 毛蕊异黄酮 /
- 肠道菌群
Abstract: OBJECTIVE To study the effects of Radix Saposhnikoviae on the metabolism of calycosin-7-O-β-D-glucoside as the main active component in Radix Astragali in intestinal flora of rats， exploring the scientific connotation of compatible application of Radix Astragali-Radix Saposhnikovia from the perspective of metabolism. METHODS The concentrations of calycosin-7-O-β-D-glucoside and calycosin between Radix Astragali group and Radix Astragali-Radix Saposhnikoviae group， as well as calycosin-7-O-β-D-glucoside monomer group and calycosin-7-O-β-D-glucoside-Radix Saposhnikoviae group at different time points were compared， respectively， by intestinal flora experiments. RESULTS Compared to Radix Astragali group， the concentration of calycosin-7-O-β-D-glucoside significantly increased in Radix Astragali-Radix Saposhnikoviae group (P<0.01)， while the concentration of calycosin significantly decreased (P<0.01) at different time points. Compared to monomer group， the concentration of calycosin-7-O-β-D-glucoside was significantly higher than that in calycosin-7-O-β-D-glucoside-Radix Saposhnikoviae group (P<0.01) and the concentration of calycosin was significantly lower (P<0.01). CONCLUSION Radix Saposhnikoviae can inhibit the transformation from calycosin-7-O-β-D-glucoside to calycosin and slow its metabolism.
- Radix Saposhnikoviae /
- Radix Astragali /
- calycosin-7-O-β-D-glucoside /
- calycosin /
- intestinal flora

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参考文献(8)

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