通腑泄热法对脑膜炎发热大鼠退热及免疫调控作用研究
Antipyretic Effects and Immune Regulation Function of Tongfu Xiere Therapy in Fever Rats with Meningitis
-
摘要: 目的 基于脑-肠轴学说,探讨通腑泄热法对脑膜炎发热大鼠的退热效应及免疫调控作用。方法 采用埃希氏大肠杆菌小脑延髓池注射建立大鼠细菌性脑膜炎发热模型。实验动物随机分为6组:正常组、模型组、大承气汤组、醒脑静注射液组、大承气汤合醒脑静注射液组、扑热息痛组。观察各组用药后的退热效果,同时检测血浆中IFN-γ、IL-4的含量变化,及组织、血浆中P物质的含量。结果 大承气汤和醒脑静注射液及大承气汤合醒脑静注射液组均能有效治疗脑膜炎发热,与模型组比较有显著的解热效果(P<0.05),能降低血浆中IFN-γ(P<0.01)、IL-4(P<0.05)水平,下调IFN-γ/IL-4的比值(P<0.05),并且能降低发热大鼠P物质含量(P<0.05)。结论 通腑泄热法对脑膜炎发热有较好的解热效果,这可能与其通过脑肠轴元件生物介质调节机体免疫功能有密切关系。Abstract: OBJECTIVE Based on the brain-gut axis theory, to investigate the antipyretic effect and immune regulation function of Tongfu Xiere therapy in fever rats with meningitis. METHODS The rat fever model induced bacterial Meninggitis was established with injecting the Escherichia coli into cerebellomedullary cistern. The experimental animals were divided into six groups: the normal group, the model group, Dachengqi decoction group, Xingnaojing injection group, Dachengqi decoction+Xingnaojing injection group and paracetamol group. Antipyretic effect of each drug was observed by measuring body temperature after treatment. The levels of IFN-γ, IL-4 and SP in plasma were detected by enzyme-linked immunosorbent assay. RESULTS Compared to the model group, Dachengqi decoction group, Xingnaojing injection group and Dachengqi decoction+Xingnaojing injection group significantly reduced the temperature of the fever rats(P<0.05), the levels of IFN-γ(P<0.01), IL-4(P<0.05)and SP(P<0.05), and the ratio of IFN-γ/IL-4(P<0.05). CONCLUSION Tongfu Xiere therapy shows antipyretic effect on fever rats with Meningitis, which is related to immune regulation biologically mediated by brain-gut axis components.
-
Key words:
- Tongfu Xiere therapy /
- brain-gut axis /
- meningitis fever /
- antipyretic effect /
- immune regulation
-
[1] 岳少杰,李乔俊,罗自强,等.清开灵注射液辅助治疗对兔大肠杆菌脑膜炎脑损伤保护作用及机制[J].中国中西医结合杂志,2005, 25(7):633-636. [2] Yue SJ, Li QJ, Luo ZQ, et al. The neuroprotective effects and its mechanisms of qingkailing injection on bacterial meningitis induced by E. coli in rabbits[J]. Chin J Integr Tradit Western Med, 2005, 25(7): 633-636. [3] Pan WH, Kastin AJ. Urocortion and the brain[J]. Progress in Neurobiology, 2008,84:148-156. [4] Clark WG, Lipton JM. Brain and pituitary peptides in thermoregulation[J]. Pharmacol Ther, 1983, 22(2): 249-297. [5] 陈颖,冯甲棣.P物质对大鼠PO/AH温度-性敏感神经元放电活动的影响[J].中国医科大学学报,2001, 30(1):22-24. [6] Chen Y, Feng JD. Effect of SP on electroactivities of Thermo-Estradiol related sensitive neurons in PO/AH of rats[J]. J China Med Univ, 2001, 30(1): 22-24. [7] Payan DG, Brewster DR, Goetzl EJ. Specific stimulation of human T lymphocytes by substance P[J]. J Immunol, 1983, 131(4): 1613-1615. [8] 张世红,赵晏.P物质的免疫调节作用[J].生理科学进展,2002, 33(3):235-238. [9] Zhang SH, Zhao Y. The immune regulatory effects of substance P[J]. Progr Physiol Sci, 2002, 33(3): 235-238.
点击查看大图
计量
- 文章访问数: 977
- HTML全文浏览量: 27
- PDF下载量: 1114
- 被引次数: 0