川芎多糖对人肝癌细胞HepG2增殖及凋亡的影响
Influence of Chuanxiong Polysaccharide on Proliferation and Apoptosis of Human Hepatoma Cell HepG2
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摘要: 目的 探讨川芎多糖抑制人肝癌细胞株HepG2增殖，诱导细胞凋亡的作用机制。方法 体外培养人肝癌细胞株HepG2，加入不同浓度的川芎多糖，经MTT法检测HepG2细胞的活性，观察药物作用后的细胞形态，并采用流式细胞术检测HepG2细胞周期。结果 HepG2细胞的存活率随给药浓度的增大而降低，抑制作用呈现出明显的剂量依赖性(P<0.05)；和空白组比较，药物作用48 h后给药组G1期细胞百分含量明显升高(P<0.01)，S期细胞百分含量明显减少(P<0.01)。结论 川芎多糖对人肝癌HepG2细胞有明显的体外活性抑制作用，可能是通过将肿瘤细胞阻滞在G1期，从而诱导它的凋亡。Abstract: OBJECTIVE To investigate the mechanism of Chuanxiong polysaccharide inhibiting proliferation and inducing apoptosis of human hepatoma cell HepG2. METHODS Human hepatoma cell line HepG2 were cultured in vitro， added with different concentrations of Chuanxiong polysaccharide. HepG2 cell activity was detected in MTT method; cell morphology was observed and HepG2 cell cycle was detected in flow cytometry. RESULTS HepG2 cell viability reduced with increasing concentration and the inhibitory effect showed a significant dose-dependent (P<0.05); compared with blank group， after 48h， percentage of cells in drug-administered group in G1 phase significantly increased (P<0.01) while the percentage of cells in S phase significantly reduced(P<0.01). CONCLUSION Chuanxiong polysaccharide can significantly inhibit human hepatoma HepG2 cells in vitro and inducing apoptosis by arresting tumor cells in G1 phase.
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Key words:
- Chuanxiong polysaccharide /
- sevag /
- HepG2 /
- cell apoptosis /
- MTT
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