碱性成纤维细胞生长因子壳聚糖微球的研制

南京医科大学第一附属医院口腔科江苏南京210029(卜寿山, 曹晓健), 华中科技大学同济医学院湖北武汉430000(方华丰), 南京中医药大学附属南京市中医院口腔科江苏南京210001(许金菊), 江苏省药物研究所江苏南京210009(徐兵), 南京医科大学第一附属医院口腔科江苏南京210029(尹林)

基金项目: 江苏省教育厅自然科学基金 (0 1KJB3 2 0 0 0 4)

Research and Development of Chitosan Microballoons of Alkaline Collagenoblast Growth Factor

摘要: 目的　探讨制备壳聚糖微球包裹碱性成纤维细胞生长因子 (bFGF)的方法。方法　采用Berthold的沉淀 /凝聚法制备壳聚糖微球 ,用此微球包裹bFGF ,对bFGF壳聚糖微球的大小、形态、含量和体外释药进行研究。结果　空白壳聚糖微球表面光滑 ,粒径范围 1.2～ 3.8μm。载药量约为 4 .6 8× 10 4U/mg ,包封率为 93.7%。体外释放试验显示 ,壳聚糖微球释放bFGF初期释药较快 ,尤其d 1有突释现象 (累计释药度约占 18.6 % ) ,随后bFGF释放速度逐渐变缓 ,d 10累计释放度约5 2 % ,d 2 0约 6 1.5 %。结论　bFGF壳聚糖微球具有较高的包封率和显著的缓释bFGF作用。
- 壳聚糖 /
- 微球研制 /
- 成纤维细胞生长因子 /
- 碱性

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