复方辛夷平喘液对实验性哮喘模型及人胚肺细胞抗氧化损伤的实验研究
Effect of Compound Xinyi Pingchuan Liquid in Preventing Oxidative Damage of Experimental Asthma Model and Human Embryonic Lung Cell
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摘要: 目的 观察中药复方辛夷平喘液抗氧化性损伤的作用。方法 36只雌雄各半豚鼠随机分为模型组、阳性对照组、空白组和 3个受试药剂量组 ,共 6组。卵清蛋白 (OVA)制成哮喘模型 ,观察各组SOD、MDA水平的变化。将受试药 3个剂量按比例分别加入对数生长期的人胚肺细胞中 ,设阳性对照组和空白对照组 ,药物作用 2 4h后 ,消化细胞制成细胞悬液 ,观察对SOD水平的影响。结果 哮喘组豚鼠SOD水平低于用药组 ,MDA高于用药组 (P <0 .0 5 )。人胚肺细胞用药各组SOD水平与对照组相比明显上升 (P <0 .0 1)。结论 复方辛夷平喘液具有抗氧化性损伤的作用Abstract: OBJECTIVE To observe the effect of Compound Xinyi Pichuan Liquid in preventing oxidative damage. METHOD 36 male and female guinea pigs were randomly divided into a model group, a positive control group, a blank control group and three treatment groups with different dosages of medication. Egg albumen was used to make asthma models to observe the changes of SOD and MDA level. Three different dosages of drugs at different ratio were added to human embryonic lung cells to be compared with the positive control group and the blank control group. 24 hours after the use of drugs, the digestion cells were made into cell suspension to observe the changes in SOD level. RESULT The guinea pig SOD level in the asthma group was lower than the medication groups and the MDA level was higher than the medication groups, with marked significance as compared with the medication group in which large and medium dosages were used (P<0.05). The SOD level in the medication groups in which human embryonic lung cells were used increased as compared with the control group (P<0.01). CONCLUSION Compound Xinyi Pingchuan Liquid has the effect of preventing oxidative damage.
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